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The map label for this gene is gidA1

Identifier: 49475384

GI number: 49475384

Start: 695879

End: 697285

Strand: Reverse

Name: gidA1

Synonym: BH05920

Alternate gene names: 49475384

Gene position: 697285-695879 (Counterclockwise)

Preceding gene: 49475390

Following gene: 49475376

Centisome position: 36.11

GC content: 41.29

Gene sequence:

>1407_bases
ATGTTCAATAAACTCAGCACTCCAATTCATATCATCGGCGGCGGTCTTGCTGGATGTGAAGCAAGCTGGCAAATCGCTCA
ATCAGGAATTCCCGTTATCTTACATGAAATGCGGCCCCAAAAAAAATCTGAGGCCCATAAAACCGGCCAGCTTGCCGAAC
TAGTATGTTCAAACTCATTTCGTTCTGATGATTCCTCAACAAATGCTGTAGGCCTTCTCCATACTGAAATGCGATTGGCA
AAGTCCTTAATTATGAAGGCTGCAGATGCTAATAAGGTACCAGCAGGAAGTGCGCTTGCCGTTGATCGTGATGGATTTTC
AAAAACCGTTACGGCAGCGCTCGAAAATCATCCTCTTATAACAATAAAGCGTGAAGAAATTCAAGAGATTCCTGAGAATT
GGCCTCACATTATCATTGCAACAGGCCCCCTTACCTCACCAAAACTCGCTAAAGCAATCCAAACAATAACCGGAACAGAA
GCTCTCTCTTTTTTTGATGCTATCGCACCTATTATCTATACCGATAGTATTAATATGGATATTTGTTGGTATCAATCGCG
CTATGATAAAATCGGTCCTGAGGGAACAGGAAAGGATTATCTTAATTGTCCCCTTAATAAAGAACAATATGAAGCATTTG
TTCAAGCGTTGAAAAATGCGGAAAAAACAGAATTCCGCGACTTTGAAAAAACACCCTATTTTGATGGATGCTTACCAATC
GAAGTTATGGCGGAACGCGGACTTGAAACGCTCAGACATGGTCCCATGAAGCCTATGGGATTAACTAATGCTCATAATCC
CACAGTTAAAGCTTATGCCGTCGTCCAATTACGCCAAGACAATAAGCTTGGAACCCTTTATAATATGGTAGGCTTTCAAA
CAAAATTGAAATATGGCGAACAAGTTCGCATTTTTAGAATGATTCCCGGTCTTGAGAACGCAGAGTTCGCTCGCCTTGGT
GGACTTCACCGTAATACCTATCTCAACTCACCAATCATTCTTGATCAAACTCTTCGTTTAAAACAAAGGCCTGGACTGCG
CTTTGCAGGGCAAATTACCGGATGTGAGGGGTATGTAGAATCATCAGCAATAGGGCTTCTTGCTGGACGTTTTGCCGCTG
CTGAATATCACTATAATTGCCCTTGCCTACCACCATTAACCACAGCATTGGGAGCTCTCTTGAACCATATTACCGGTGGA
CATATCGTAGATGAAAAAACAGGAAGAAAATCTTTTCAACCAATGAACATCAATTTTGGACTTTTCCCTCCTATTGCTTC
TGCTTCTTATTCAGGAAAACGTTTGCCAAGTAAAGAAAAAAAATTAGCAAAAAAACAAGCAATAACTGCCAGAGCTCTTA
ACACTTGCATTCAGTGGCTAATCAACGAAGAAAAAAATAAACTCTAG

Upstream 100 bases:

>100_bases
AAACAAATATATTCAAAAAATCAAATACATATATTCTCACAAATTACAAAAACAAAAATCTATACTTTTCAGATAAGACA
GCTTCCGCTAAAAGTAAATT

Downstream 100 bases:

>100_bases
ATCAATCACCATTAACCAATTGTTGTCTTTGATTTTCATGATACAGATGATATCTTTCGCCCTTCGATCTATTTAGATCG
ACGTCATTTACCCTCAATAA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MFNKLSTPIHIIGGGLAGCEASWQIAQSGIPVILHEMRPQKKSEAHKTGQLAELVCSNSFRSDDSSTNAVGLLHTEMRLA
KSLIMKAADANKVPAGSALAVDRDGFSKTVTAALENHPLITIKREEIQEIPENWPHIIIATGPLTSPKLAKAIQTITGTE
ALSFFDAIAPIIYTDSINMDICWYQSRYDKIGPEGTGKDYLNCPLNKEQYEAFVQALKNAEKTEFRDFEKTPYFDGCLPI
EVMAERGLETLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNKLGTLYNMVGFQTKLKYGEQVRIFRMIPGLENAEFARLG
GLHRNTYLNSPIILDQTLRLKQRPGLRFAGQITGCEGYVESSAIGLLAGRFAAAEYHYNCPCLPPLTTALGALLNHITGG
HIVDEKTGRKSFQPMNINFGLFPPIASASYSGKRLPSKEKKLAKKQAITARALNTCIQWLINEEKNKL

Sequences:

>Translated_468_residues
MFNKLSTPIHIIGGGLAGCEASWQIAQSGIPVILHEMRPQKKSEAHKTGQLAELVCSNSFRSDDSSTNAVGLLHTEMRLA
KSLIMKAADANKVPAGSALAVDRDGFSKTVTAALENHPLITIKREEIQEIPENWPHIIIATGPLTSPKLAKAIQTITGTE
ALSFFDAIAPIIYTDSINMDICWYQSRYDKIGPEGTGKDYLNCPLNKEQYEAFVQALKNAEKTEFRDFEKTPYFDGCLPI
EVMAERGLETLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNKLGTLYNMVGFQTKLKYGEQVRIFRMIPGLENAEFARLG
GLHRNTYLNSPIILDQTLRLKQRPGLRFAGQITGCEGYVESSAIGLLAGRFAAAEYHYNCPCLPPLTTALGALLNHITGG
HIVDEKTGRKSFQPMNINFGLFPPIASASYSGKRLPSKEKKLAKKQAITARALNTCIQWLINEEKNKL
>Mature_468_residues
MFNKLSTPIHIIGGGLAGCEASWQIAQSGIPVILHEMRPQKKSEAHKTGQLAELVCSNSFRSDDSSTNAVGLLHTEMRLA
KSLIMKAADANKVPAGSALAVDRDGFSKTVTAALENHPLITIKREEIQEIPENWPHIIIATGPLTSPKLAKAIQTITGTE
ALSFFDAIAPIIYTDSINMDICWYQSRYDKIGPEGTGKDYLNCPLNKEQYEAFVQALKNAEKTEFRDFEKTPYFDGCLPI
EVMAERGLETLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNKLGTLYNMVGFQTKLKYGEQVRIFRMIPGLENAEFARLG
GLHRNTYLNSPIILDQTLRLKQRPGLRFAGQITGCEGYVESSAIGLLAGRFAAAEYHYNCPCLPPLTTALGALLNHITGG
HIVDEKTGRKSFQPMNINFGLFPPIASASYSGKRLPSKEKKLAKKQAITARALNTCIQWLINEEKNKL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_BARHE (Q6G3Y9)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033425.1
- ProteinModelPortal:   Q6G3Y9
- GeneID:   2864737
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH05920
- NMPDR:   fig|283166.1.peg.553
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   BHEN283166:BH05920-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 51655; Mature: 51655

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKLSTPIHIIGGGLAGCEASWQIAQSGIPVILHEMRPQKKSEAHKTGQLAELVCSNSF
CCCCCCCCEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
RSDDSSTNAVGLLHTEMRLAKSLIMKAADANKVPAGSALAVDRDGFSKTVTAALENHPLI
CCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEE
TIKREEIQEIPENWPHIIIATGPLTSPKLAKAIQTITGTEALSFFDAIAPIIYTDSINMD
EEEHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEE
ICWYQSRYDKIGPEGTGKDYLNCPLNKEQYEAFVQALKNAEKTEFRDFEKTPYFDGCLPI
EEEECHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHH
EVMAERGLETLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNKLGTLYNMVGFQTKLKYGE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
QVRIFRMIPGLENAEFARLGGLHRNTYLNSPIILDQTLRLKQRPGLRFAGQITGCEGYVE
EEEEEEECCCCCCCCHHHHCCCHHCCCCCCCEEECHHHHHHCCCCCEEEEEECCCCCHHH
SSAIGLLAGRFAAAEYHYNCPCLPPLTTALGALLNHITGGHIVDEKTGRKSFQPMNINFG
HHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECCCC
LFPPIASASYSGKRLPSKEKKLAKKQAITARALNTCIQWLINEEKNKL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFNKLSTPIHIIGGGLAGCEASWQIAQSGIPVILHEMRPQKKSEAHKTGQLAELVCSNSF
CCCCCCCCEEEEECCCCCCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
RSDDSSTNAVGLLHTEMRLAKSLIMKAADANKVPAGSALAVDRDGFSKTVTAALENHPLI
CCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEE
TIKREEIQEIPENWPHIIIATGPLTSPKLAKAIQTITGTEALSFFDAIAPIIYTDSINMD
EEEHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEE
ICWYQSRYDKIGPEGTGKDYLNCPLNKEQYEAFVQALKNAEKTEFRDFEKTPYFDGCLPI
EEEECHHHHCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHH
EVMAERGLETLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNKLGTLYNMVGFQTKLKYGE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
QVRIFRMIPGLENAEFARLGGLHRNTYLNSPIILDQTLRLKQRPGLRFAGQITGCEGYVE
EEEEEEECCCCCCCCHHHHCCCHHCCCCCCCEEECHHHHHHCCCCCEEEEEECCCCCHHH
SSAIGLLAGRFAAAEYHYNCPCLPPLTTALGALLNHITGGHIVDEKTGRKSFQPMNINFG
HHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECCCC
LFPPIASASYSGKRLPSKEKKLAKKQAITARALNTCIQWLINEEKNKL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA