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The map label for this gene is ksgA

Identifier: 49475334

GI number: 49475334

Start: 626742

End: 627572

Strand: Reverse

Name: ksgA

Synonym: BH05400

Alternate gene names: 49475334

Gene position: 627572-626742 (Counterclockwise)

Preceding gene: 49475335

Following gene: 49475326

Centisome position: 32.5

GC content: 38.87

Gene sequence:

>831_bases
ATGCCAATAGATAGTCTCCCTCCTCTTCGTGAGGTCATTAATACATATGGATTGCAAGCGCATAAATCCTTAGGCCAAAA
TTTTCTTTTTGATCTTAATTTAACATCTAAAATTGCTCATCAAGCAGGAAATATAGAAGGAAAACCCGTTATTGAAGTGG
GCCCAGGTCCTGGAGGTCTGACACGCGCATTGCTTGCAAAAGGTGCCATTGTAACAGCAATAGAGCGTGATGAGCGTTGT
ATACCAGCTCTCTTAGAGATAGAAAAACATTATCCACAAAAACTAAAAATCATTTGTAATGATGCTTTAAAACAAGATTT
TTCAAAACTGTTTGAAATATCTCCAGAAAAACCACGTATTATTGCAAATCTTCCTTATAATATTGGAACACAACTCTTGT
TAAATTGGCTGTTGGCTGAACCATGGCCTCCTTTTTATGAATCAATGACATTGATGTTTCAACGTGAAGTTGCGCAACGT
ATTACGGCAAAACCTCAATCCGCCCATTATGGACGTTTGAGTGTCTTAACCGGATGGCGTACCATTGCTAAAATTGCTTT
TGACGTACCTCCTCAGGCCTTCATACCAGCACCCAAAATAACTTCTTCTGTTGTTCATATCATTCCGCGAACACAACCTC
TCACCTGCTCCGCACAAAAATTAAGCTTTGTAACAAAAACTGCTTTTGGACAAAGACGAAAAATGCTTCGCCAAAATCTT
AAAACACTTGGAGGTGAAGTACTTTTAGAAAAAGCTGGAATCGATGAAACACGCCGTGCGGAAACGCTTGAAATTTCCGA
ATTTGTGACTTTAGCTAATTTGGTGATCTAA

Upstream 100 bases:

>100_bases
GAACTGCTTTTGATATTGCTGCTAAAGGGATTGCCTCTCCTGAAAGCTTTATTTCTGCTCTTAAACTTGCACATCAACTG
GCGGAAAATAGTTTTACACC

Downstream 100 bases:

>100_bases
AAAGCGTAATTTTATTCTTTTTCAAAACGATAAGGAGATTAATCAATCTTTTCAGCAATAAGCCCATTAATAAAAGGCTC
AAGAAAGAAACATCGTTCGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MPIDSLPPLREVINTYGLQAHKSLGQNFLFDLNLTSKIAHQAGNIEGKPVIEVGPGPGGLTRALLAKGAIVTAIERDERC
IPALLEIEKHYPQKLKIICNDALKQDFSKLFEISPEKPRIIANLPYNIGTQLLLNWLLAEPWPPFYESMTLMFQREVAQR
ITAKPQSAHYGRLSVLTGWRTIAKIAFDVPPQAFIPAPKITSSVVHIIPRTQPLTCSAQKLSFVTKTAFGQRRKMLRQNL
KTLGGEVLLEKAGIDETRRAETLEISEFVTLANLVI

Sequences:

>Translated_276_residues
MPIDSLPPLREVINTYGLQAHKSLGQNFLFDLNLTSKIAHQAGNIEGKPVIEVGPGPGGLTRALLAKGAIVTAIERDERC
IPALLEIEKHYPQKLKIICNDALKQDFSKLFEISPEKPRIIANLPYNIGTQLLLNWLLAEPWPPFYESMTLMFQREVAQR
ITAKPQSAHYGRLSVLTGWRTIAKIAFDVPPQAFIPAPKITSSVVHIIPRTQPLTCSAQKLSFVTKTAFGQRRKMLRQNL
KTLGGEVLLEKAGIDETRRAETLEISEFVTLANLVI
>Mature_275_residues
PIDSLPPLREVINTYGLQAHKSLGQNFLFDLNLTSKIAHQAGNIEGKPVIEVGPGPGGLTRALLAKGAIVTAIERDERCI
PALLEIEKHYPQKLKIICNDALKQDFSKLFEISPEKPRIIANLPYNIGTQLLLNWLLAEPWPPFYESMTLMFQREVAQRI
TAKPQSAHYGRLSVLTGWRTIAKIAFDVPPQAFIPAPKITSSVVHIIPRTQPLTCSAQKLSFVTKTAFGQRRKMLRQNLK
TLGGEVLLEKAGIDETRRAETLEISEFVTLANLVI

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=292, Percent_Identity=39.3835616438356, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI7657198, Length=237, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1786236, Length=259, Percent_Identity=35.1351351351351, Blast_Score=141, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=33.8983050847458, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25146882, Length=202, Percent_Identity=31.1881188118812, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=188, Percent_Identity=34.0425531914894, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21357273, Length=297, Percent_Identity=34.006734006734, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI21358017, Length=241, Percent_Identity=30.2904564315353, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_BARHE (Q6G438)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033375.1
- ProteinModelPortal:   Q6G438
- SMR:   Q6G438
- GeneID:   2865576
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH05400
- NMPDR:   fig|283166.1.peg.503
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q6G438
- ProtClustDB:   PRK00274
- BioCyc:   BHEN283166:BH05400-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30750; Mature: 30619

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 101-101 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIDSLPPLREVINTYGLQAHKSLGQNFLFDLNLTSKIAHQAGNIEGKPVIEVGPGPGGL
CCCCCCCHHHHHHHHHCCHHHHHCCCCEEEECCHHHHHHHHCCCCCCCEEEEECCCCCHH
TRALLAKGAIVTAIERDERCIPALLEIEKHYPQKLKIICNDALKQDFSKLFEISPEKPRI
HHHHHHCCHHEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IANLPYNIGTQLLLNWLLAEPWPPFYESMTLMFQREVAQRITAKPQSAHYGRLSVLTGWR
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TIAKIAFDVPPQAFIPAPKITSSVVHIIPRTQPLTCSAQKLSFVTKTAFGQRRKMLRQNL
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KTLGGEVLLEKAGIDETRRAETLEISEFVTLANLVI
HHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIDSLPPLREVINTYGLQAHKSLGQNFLFDLNLTSKIAHQAGNIEGKPVIEVGPGPGGL
CCCCCCHHHHHHHHHCCHHHHHCCCCEEEECCHHHHHHHHCCCCCCCEEEEECCCCCHH
TRALLAKGAIVTAIERDERCIPALLEIEKHYPQKLKIICNDALKQDFSKLFEISPEKPRI
HHHHHHCCHHEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IANLPYNIGTQLLLNWLLAEPWPPFYESMTLMFQREVAQRITAKPQSAHYGRLSVLTGWR
EEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TIAKIAFDVPPQAFIPAPKITSSVVHIIPRTQPLTCSAQKLSFVTKTAFGQRRKMLRQNL
HHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KTLGGEVLLEKAGIDETRRAETLEISEFVTLANLVI
HHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA