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The map label for this gene is purF [H]

Identifier: 49475319

GI number: 49475319

Start: 610981

End: 612507

Strand: Reverse

Name: purF [H]

Synonym: BH05240

Alternate gene names: 49475319

Gene position: 612507-610981 (Counterclockwise)

Preceding gene: 49475320

Following gene: 49475318

Centisome position: 31.72

GC content: 39.88

Gene sequence:

>1527_bases
ATGAACCGTTTCTCACATCATAATCGAAAGAATACGATGACAAAAATCGACATTCCATTTCAGGAGTTTTCATTGAATGA
CGATACCCTTCATGAAGAATGCGGAGTCTTTGGTATTCTTGGCCATGAGGATGCAGCGACTTTAACAGCTCTTGGACTTC
ACGCGCTTCAACATCGTGGACAAGAAGCAGCTGGGATTGTTTCTTACCATAATAAAATGTTTCATCAAGAAAAGCATCTA
GGTCTTGTTGGTGATCATTATACAAACCCCGCAACACTTGCGCGTTTACCGGGAAATCGCGCTATCGGGCATACCCGTTA
TTCAACAACTGGAGAAATAGCATTACGCAATGTGCAACCCCTTTTTGCTGAGTTAAAAGCTGGAGGTATTGCCATTGCCC
ATAATGGCAATCTTACTAACGGTCTTACATTACGCCGAGAACTCATTGCTTCCGGTGCTATCTGCCAATCAACATCAGAT
TCTGAAGTTTTTCTTCATCTTATTGCTCGTTCACGTTATGAATCGTCGTCAGATCGCTTTGTTGATGCTATTCGAAAAGT
GGAAGGTGGTTATGCCATGTTAGCGCTCACACGCACCAAACTGATTGCTGCGCGTGATCCAATAGGTATTCGACCTCTGG
TGATGGGTGAACTTCATGGTAAACCAATATTTTGTTCCGAAACCTGTGCCCTTGATATTATTGGAGCAAAATATATTCGC
GATGTTAAAAATGGAGAAGTCATCGTATGTGAAATACAAAAAAATGGGGAAATCACCACAAAAATTATAAAACCAGAAAA
TAAGAAACCAGAAAAACTTTGCCTTTTTGAATATGTCTATTTTGCGCGTCCTGATTCAATCGTCGGAGGACGAAGTGTTT
ATATGACACGTAAAAATATGGGTATCCGTTTAGCTCAAGAAGCCCCCTGTGAAGGTGATATTGTGGTTCCTGTTCCTGAC
GGGGGAACACCCGCTGCAATTGGTTATGCCCAAGAAATTGGAATTCCCTTTGAGCTTGGTATTATTCGTAATCATTACGT
CGGACGCACATTTATCGAACCAACACAACAAATTCGCGCTTTTGGGGTTAAATTAAAGCATTCTGCAAATCGTCCTGTTA
TTGAAGGAAAACGTGTCATTCTGGTCGATGACTCCATTGTACGAGGAACAACATCCGTTAAAATTGTGCAAATGCTCCGC
GATGCAGGAGCAAAAGAAGTTCATATGCGCATCTCTAGTCCTATGATTTTCTATCCCGATTTCTATGGTATTGATACACC
TAAAGTTGAAAGTTTGTTGGCTAATCAATATCCAGATTTAAAAGCTATGTGCAATTTTGTTGGAGCTGACTCATTAGAAT
TTCTTTCAACAAATGGTCTATATCTTGCTGTGATAGGAGAAAAACGAAATAATGCTGATCCACAATTTACTGACCATTAT
TTTACTGGAGATTATCCTACACATCTAGTCGATCAAGAAAGTATTCCTAAAATTTATCAATCATCTGTCCTAAAAACAAG
AGATTAA

Upstream 100 bases:

>100_bases
TGCAAACACAAATGATCAACATTCTCATGAAAATATGTCCCAACAAAATGGAAAATAATTTGTCATTGAGGAAAAAATTC
AGATAATACTAATGCATGAT

Downstream 100 bases:

>100_bases
GGATGAAAAAACTTGATTTTAGTCTCTTTGGTCGTGTTGCACTCGTTACCGGTGCTTCAAGAGGAATTGGATATCATTTA
GCATTAGAGCTTGCAGAACG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNRFSHHNRKNTMTKIDIPFQEFSLNDDTLHEECGVFGILGHEDAATLTALGLHALQHRGQEAAGIVSYHNKMFHQEKHL
GLVGDHYTNPATLARLPGNRAIGHTRYSTTGEIALRNVQPLFAELKAGGIAIAHNGNLTNGLTLRRELIASGAICQSTSD
SEVFLHLIARSRYESSSDRFVDAIRKVEGGYAMLALTRTKLIAARDPIGIRPLVMGELHGKPIFCSETCALDIIGAKYIR
DVKNGEVIVCEIQKNGEITTKIIKPENKKPEKLCLFEYVYFARPDSIVGGRSVYMTRKNMGIRLAQEAPCEGDIVVPVPD
GGTPAAIGYAQEIGIPFELGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRPVIEGKRVILVDDSIVRGTTSVKIVQMLR
DAGAKEVHMRISSPMIFYPDFYGIDTPKVESLLANQYPDLKAMCNFVGADSLEFLSTNGLYLAVIGEKRNNADPQFTDHY
FTGDYPTHLVDQESIPKIYQSSVLKTRD

Sequences:

>Translated_508_residues
MNRFSHHNRKNTMTKIDIPFQEFSLNDDTLHEECGVFGILGHEDAATLTALGLHALQHRGQEAAGIVSYHNKMFHQEKHL
GLVGDHYTNPATLARLPGNRAIGHTRYSTTGEIALRNVQPLFAELKAGGIAIAHNGNLTNGLTLRRELIASGAICQSTSD
SEVFLHLIARSRYESSSDRFVDAIRKVEGGYAMLALTRTKLIAARDPIGIRPLVMGELHGKPIFCSETCALDIIGAKYIR
DVKNGEVIVCEIQKNGEITTKIIKPENKKPEKLCLFEYVYFARPDSIVGGRSVYMTRKNMGIRLAQEAPCEGDIVVPVPD
GGTPAAIGYAQEIGIPFELGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRPVIEGKRVILVDDSIVRGTTSVKIVQMLR
DAGAKEVHMRISSPMIFYPDFYGIDTPKVESLLANQYPDLKAMCNFVGADSLEFLSTNGLYLAVIGEKRNNADPQFTDHY
FTGDYPTHLVDQESIPKIYQSSVLKTRD
>Mature_508_residues
MNRFSHHNRKNTMTKIDIPFQEFSLNDDTLHEECGVFGILGHEDAATLTALGLHALQHRGQEAAGIVSYHNKMFHQEKHL
GLVGDHYTNPATLARLPGNRAIGHTRYSTTGEIALRNVQPLFAELKAGGIAIAHNGNLTNGLTLRRELIASGAICQSTSD
SEVFLHLIARSRYESSSDRFVDAIRKVEGGYAMLALTRTKLIAARDPIGIRPLVMGELHGKPIFCSETCALDIIGAKYIR
DVKNGEVIVCEIQKNGEITTKIIKPENKKPEKLCLFEYVYFARPDSIVGGRSVYMTRKNMGIRLAQEAPCEGDIVVPVPD
GGTPAAIGYAQEIGIPFELGIIRNHYVGRTFIEPTQQIRAFGVKLKHSANRPVIEGKRVILVDDSIVRGTTSVKIVQMLR
DAGAKEVHMRISSPMIFYPDFYGIDTPKVESLLANQYPDLKAMCNFVGADSLEFLSTNGLYLAVIGEKRNNADPQFTDHY
FTGDYPTHLVDQESIPKIYQSSVLKTRD

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=472, Percent_Identity=39.1949152542373, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI1788651, Length=481, Percent_Identity=39.9168399168399, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790167, Length=213, Percent_Identity=30.5164319248826, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=38.6363636363636, Blast_Score=287, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6323958, Length=487, Percent_Identity=35.9342915811088, Blast_Score=236, Evalue=8e-63,
Organism=Drosophila melanogaster, GI28573187, Length=505, Percent_Identity=41.1881188118812, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24659598, Length=498, Percent_Identity=40.1606425702811, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24659604, Length=477, Percent_Identity=40.461215932914, Blast_Score=319, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56289; Mature: 56289

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFSHHNRKNTMTKIDIPFQEFSLNDDTLHEECGVFGILGHEDAATLTALGLHALQHRG
CCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCC
QEAAGIVSYHNKMFHQEKHLGLVGDHYTNPATLARLPGNRAIGHTRYSTTGEIALRNVQP
CCHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEECCHH
LFAELKAGGIAIAHNGNLTNGLTLRRELIASGAICQSTSDSEVFLHLIARSRYESSSDRF
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCHHHH
VDAIRKVEGGYAMLALTRTKLIAARDPIGIRPLVMGELHGKPIFCSETCALDIIGAKYIR
HHHHHHHCCCEEEEEEEEEEEEEECCCCCCCCEEEECCCCCCEEECCCCEEEEHHHHHHH
DVKNGEVIVCEIQKNGEITTKIIKPENKKPEKLCLFEYVYFARPDSIVGGRSVYMTRKNM
HCCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEEEHHHHCCCCCCCCCCEEEEEECCC
GIRLAQEAPCEGDIVVPVPDGGTPAAIGYAQEIGIPFELGIIRNHYVGRTFIEPTQQIRA
CEEEECCCCCCCCEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCHHCCHHHHHHH
FGVKLKHSANRPVIEGKRVILVDDSIVRGTTSVKIVQMLRDAGAKEVHMRISSPMIFYPD
HCEEEECCCCCCEECCCEEEEECCCEECCCHHHHHHHHHHHCCCCEEEEEECCCEEECCC
FYGIDTPKVESLLANQYPDLKAMCNFVGADSLEFLSTNGLYLAVIGEKRNNADPQFTDHY
CCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCE
FTGDYPTHLVDQESIPKIYQSSVLKTRD
ECCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRFSHHNRKNTMTKIDIPFQEFSLNDDTLHEECGVFGILGHEDAATLTALGLHALQHRG
CCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCC
QEAAGIVSYHNKMFHQEKHLGLVGDHYTNPATLARLPGNRAIGHTRYSTTGEIALRNVQP
CCHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEECCHH
LFAELKAGGIAIAHNGNLTNGLTLRRELIASGAICQSTSDSEVFLHLIARSRYESSSDRF
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHCCCCCHHHH
VDAIRKVEGGYAMLALTRTKLIAARDPIGIRPLVMGELHGKPIFCSETCALDIIGAKYIR
HHHHHHHCCCEEEEEEEEEEEEEECCCCCCCCEEEECCCCCCEEECCCCEEEEHHHHHHH
DVKNGEVIVCEIQKNGEITTKIIKPENKKPEKLCLFEYVYFARPDSIVGGRSVYMTRKNM
HCCCCCEEEEEEECCCCEEEEEECCCCCCCCCEEEEEHHHHCCCCCCCCCCEEEEEECCC
GIRLAQEAPCEGDIVVPVPDGGTPAAIGYAQEIGIPFELGIIRNHYVGRTFIEPTQQIRA
CEEEECCCCCCCCEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCCCCCHHCCHHHHHHH
FGVKLKHSANRPVIEGKRVILVDDSIVRGTTSVKIVQMLRDAGAKEVHMRISSPMIFYPD
HCEEEECCCCCCEECCCEEEEECCCEECCCHHHHHHHHHHHCCCCEEEEEECCCEEECCC
FYGIDTPKVESLLANQYPDLKAMCNFVGADSLEFLSTNGLYLAVIGEKRNNADPQFTDHY
CCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCE
FTGDYPTHLVDQESIPKIYQSSVLKTRD
ECCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA