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The map label for this gene is nusA [H]

Identifier: 49475029

GI number: 49475029

Start: 292854

End: 294440

Strand: Reverse

Name: nusA [H]

Synonym: BH02170

Alternate gene names: 49475029

Gene position: 294440-292854 (Counterclockwise)

Preceding gene: 49475030

Following gene: 49475028

Centisome position: 15.25

GC content: 41.84

Gene sequence:

>1587_bases
ATGGCTACAAGCGCTAACAGGCTTGAAATTCTGCAAATTGCAGATGCTGTTGCACGTGAAAAGTCGATTGACCGTGAAAT
TGTCATTTCTGCGATGGCCGATGCTATTCAGAAAGCGGCGCGTTCTCGTTATGGTCAAGAAACCAACATCCGTGCTGAGA
TCAATTCAAAAACAGGTGAAATTAAATTACAGCGTCTCCTTAAAATTGTTGATATCATTGAAGACTATACAAGTGAAATT
ACTTTATCTGATGCACTCAAACGTCAAGCGGATGCTAAAGTGGGTGATTTTTTCGCCGATCTTTTACCCCCCATGGATTT
TGGACGCATCGCTGCGCAATCTGCTAAACAAGTTATTGTACAAAAAGTACGCGATGCAGAGCGTGAACAACAATATGAAG
AATTTAAAAATAAAATTGGTGAAATTATTTCCGGTACGGTAAAACGTGTCGAATATGGCAATGTTATCGTTGATCTAGGA
CGCGGTGAAGCTATTGTGCGTCGTGATGAATTAATTCCACGTGAATCTTTCCGCTATGGAGATCGTATTCGCGCCTTTGT
TCACGATGTGCATCGCGAACCACGCGGTCCGCAAATTTTCCTTTCACGCACACATCCCCAGTTTATGGTAAAACTTTTTA
CCATGGAAGTGCCAGAAATTTATGATGGTATTATAGAAATCAAATCCGTTGCCCGCGATCCAGGTTCACGTGCTAAAATT
GCGGTTGTCTCACGCGATGGCTCGATTGATCCCGTTGGAGCATGCGTTGGAATGCGAGGAAGTCGTGTACAAGCTGTTGT
TGCAGAATTACAAGGCGAAAAAATTGATATTATTCCTTGGTCACCTGATGCTGCAACATTTATCGTCAATGCACTACAAC
CCGCCGAAGTTTCTAAAGTCGTCCTCGATGAAGATGCTGAACGTATTGAAGTTATTGTGCCTGATGACCAGCTTAGCCTT
GCTATTGGTCGGCGTGGACAAAATGTCCGTTTAGCTTCCCAACTAACAGGATGGAATATTGATATCTTAACGGAGCAAGA
AGAATCTGAAAACCGTCAAAAAGAATTTACTGAACGCAGCAAACTGTTTATGGAATCTTTAGATGTCGACGAAATGATCG
GGCAGGTTATGGCATCAGAAGGCTTTTCTTCCATTGAGGAAATCGCTTATATTGATCTTGAAGAAATCGCTTCTATTGAT
GGTTTTGATCATGAAACTGCTGCTGAAATCCAAAACCGTGCACAGGAATATTTAGAACGCAAAGAAAAAGAACTTGATGA
AAAACGTAAAAAACTCGGTGTTTGTGATGAACTGCGAATGCTTCCTGGAATGACAAGTGCTATGTTGGTTGCTATTGGTG
AAGATGGTGTCAAAACAATGGAAGATTTTGCAGGCTATGCAGTCGATGATTTGGCTGGCTGGAGAGAACGTAAGGAAGGT
CAAACACAGAATTTTTCTGGTATCCTGACTCCATTCGATATTACACGCGCTGATGCTGAAGCTATGGTTTTAGCTGCACG
TGTGCAAGCAGGCTGGATAAATCAAGCTGACCTTGTTACAGAAAATTCTGCAGAAAATGAAAAATAA

Upstream 100 bases:

>100_bases
TCAGAAGATAATCAAAAACTCTCAAAACAGGAACGAAATTACAAGAATTAATATCACTGGGATCAATGCCCATCGTGTGG
TACAAAGAAGGAGAAAATCG

Downstream 100 bases:

>100_bases
ATAATTTCAATGTGGATGCATTTTAAATGAATGAACGGACCTGTATTGTGACAAGAAAAAATGCTTCAGCAAAAGCGCTG
ATCCGTTTTGTTATTGGTCC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MATSANRLEILQIADAVAREKSIDREIVISAMADAIQKAARSRYGQETNIRAEINSKTGEIKLQRLLKIVDIIEDYTSEI
TLSDALKRQADAKVGDFFADLLPPMDFGRIAAQSAKQVIVQKVRDAEREQQYEEFKNKIGEIISGTVKRVEYGNVIVDLG
RGEAIVRRDELIPRESFRYGDRIRAFVHDVHREPRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
AVVSRDGSIDPVGACVGMRGSRVQAVVAELQGEKIDIIPWSPDAATFIVNALQPAEVSKVVLDEDAERIEVIVPDDQLSL
AIGRRGQNVRLASQLTGWNIDILTEQEESENRQKEFTERSKLFMESLDVDEMIGQVMASEGFSSIEEIAYIDLEEIASID
GFDHETAAEIQNRAQEYLERKEKELDEKRKKLGVCDELRMLPGMTSAMLVAIGEDGVKTMEDFAGYAVDDLAGWRERKEG
QTQNFSGILTPFDITRADAEAMVLAARVQAGWINQADLVTENSAENEK

Sequences:

>Translated_528_residues
MATSANRLEILQIADAVAREKSIDREIVISAMADAIQKAARSRYGQETNIRAEINSKTGEIKLQRLLKIVDIIEDYTSEI
TLSDALKRQADAKVGDFFADLLPPMDFGRIAAQSAKQVIVQKVRDAEREQQYEEFKNKIGEIISGTVKRVEYGNVIVDLG
RGEAIVRRDELIPRESFRYGDRIRAFVHDVHREPRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
AVVSRDGSIDPVGACVGMRGSRVQAVVAELQGEKIDIIPWSPDAATFIVNALQPAEVSKVVLDEDAERIEVIVPDDQLSL
AIGRRGQNVRLASQLTGWNIDILTEQEESENRQKEFTERSKLFMESLDVDEMIGQVMASEGFSSIEEIAYIDLEEIASID
GFDHETAAEIQNRAQEYLERKEKELDEKRKKLGVCDELRMLPGMTSAMLVAIGEDGVKTMEDFAGYAVDDLAGWRERKEG
QTQNFSGILTPFDITRADAEAMVLAARVQAGWINQADLVTENSAENEK
>Mature_527_residues
ATSANRLEILQIADAVAREKSIDREIVISAMADAIQKAARSRYGQETNIRAEINSKTGEIKLQRLLKIVDIIEDYTSEIT
LSDALKRQADAKVGDFFADLLPPMDFGRIAAQSAKQVIVQKVRDAEREQQYEEFKNKIGEIISGTVKRVEYGNVIVDLGR
GEAIVRRDELIPRESFRYGDRIRAFVHDVHREPRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIEIKSVARDPGSRAKIA
VVSRDGSIDPVGACVGMRGSRVQAVVAELQGEKIDIIPWSPDAATFIVNALQPAEVSKVVLDEDAERIEVIVPDDQLSLA
IGRRGQNVRLASQLTGWNIDILTEQEESENRQKEFTERSKLFMESLDVDEMIGQVMASEGFSSIEEIAYIDLEEIASIDG
FDHETAAEIQNRAQEYLERKEKELDEKRKKLGVCDELRMLPGMTSAMLVAIGEDGVKTMEDFAGYAVDDLAGWRERKEGQ
TQNFSGILTPFDITRADAEAMVLAARVQAGWINQADLVTENSAENEK

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=482, Percent_Identity=42.3236514522822, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59079; Mature: 58947

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSANRLEILQIADAVAREKSIDREIVISAMADAIQKAARSRYGQETNIRAEINSKTGE
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCH
IKLQRLLKIVDIIEDYTSEITLSDALKRQADAKVGDFFADLLPPMDFGRIAAQSAKQVIV
HHHHHHHHHHHHHHHHHHCEEHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QKVRDAEREQQYEEFKNKIGEIISGTVKRVEYGNVIVDLGRGEAIVRRDELIPRESFRYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCEEEEHHHCCCHHHHHHH
DRIRAFVHDVHREPRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEE
AVVSRDGSIDPVGACVGMRGSRVQAVVAELQGEKIDIIPWSPDAATFIVNALQPAEVSKV
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCHHHHHH
VLDEDAERIEVIVPDDQLSLAIGRRGQNVRLASQLTGWNIDILTEQEESENRQKEFTERS
HHCCCCCEEEEEECCCCEEEEECCCCCCEEEHHHCCCCCEEEEECCHHHHHHHHHHHHHH
KLFMESLDVDEMIGQVMASEGFSSIEEIAYIDLEEIASIDGFDHETAAEIQNRAQEYLER
HHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHH
KEKELDEKRKKLGVCDELRMLPGMTSAMLVAIGEDGVKTMEDFAGYAVDDLAGWRERKEG
HHHHHHHHHHHCCCHHHHHHCCCCCHHEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCC
QTQNFSGILTPFDITRADAEAMVLAARVQAGWINQADLVTENSAENEK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEECCCCCCCC
>Mature Secondary Structure 
ATSANRLEILQIADAVAREKSIDREIVISAMADAIQKAARSRYGQETNIRAEINSKTGE
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCH
IKLQRLLKIVDIIEDYTSEITLSDALKRQADAKVGDFFADLLPPMDFGRIAAQSAKQVIV
HHHHHHHHHHHHHHHHHHCEEHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QKVRDAEREQQYEEFKNKIGEIISGTVKRVEYGNVIVDLGRGEAIVRRDELIPRESFRYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCCEEEEHHHCCCHHHHHHH
DRIRAFVHDVHREPRGPQIFLSRTHPQFMVKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEE
AVVSRDGSIDPVGACVGMRGSRVQAVVAELQGEKIDIIPWSPDAATFIVNALQPAEVSKV
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCHHHHHH
VLDEDAERIEVIVPDDQLSLAIGRRGQNVRLASQLTGWNIDILTEQEESENRQKEFTERS
HHCCCCCEEEEEECCCCEEEEECCCCCCEEEHHHCCCCCEEEEECCHHHHHHHHHHHHHH
KLFMESLDVDEMIGQVMASEGFSSIEEIAYIDLEEIASIDGFDHETAAEIQNRAQEYLER
HHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHH
KEKELDEKRKKLGVCDELRMLPGMTSAMLVAIGEDGVKTMEDFAGYAVDDLAGWRERKEG
HHHHHHHHHHHCCCHHHHHHCCCCCHHEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHCC
QTQNFSGILTPFDITRADAEAMVLAARVQAGWINQADLVTENSAENEK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]