Definition Bartonella henselae str. Houston-1, complete genome.
Accession NC_005956
Length 1,931,047

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The map label for this gene is pnp

Identifier: 49475022

GI number: 49475022

Start: 283004

End: 285205

Strand: Reverse

Name: pnp

Synonym: BH02100

Alternate gene names: 49475022

Gene position: 285205-283004 (Counterclockwise)

Preceding gene: 49475023

Following gene: 49475021

Centisome position: 14.77

GC content: 41.46

Gene sequence:

>2202_bases
ATGTTCAAAACACACAAGATAGAAATTGAATGGGCAGGCAGGCCACTAACCCTTGAGACAGGGAAAATAGCGCGTCAAGC
TGATGGCGCAGTCATTGCTACCTACGGTGAAACTATTGTCTTAGCAACCGTGGTATCAGCAAAAAGCCCCAAACCAGACC
AAGACTTTTTTCCACTCACCGTTAATTATCAAGAAAAATCCTATGCTGTTGGTAGAATCCCCGGTGGTTATTTAAAACGT
GAAAGCCGACCAAGTGAAAATGAAACTTTAATTTCACGTTTAATTGATCGTCCGATTCGCCCCCTCTTCGCTGACGGTTA
TAAAAATGATACACAAGTCATTGTATCTGTTATACAGCATGATCTTGAAAATAACCCAGATATTCTCGCAATGATTGCAT
CTTCTGCTGCATTAACTCTGTCAGGTGTTCCTTTCATGGGCCCCATTGCCGGAGCTCGTGTTGGCTATTGTAACGGACAA
TATATTCTCAATCCCCATATCGATGAAATGTCCGAATCAAAACTCGATCTCGTTGTTGCCGGAACAGAAAATGCTGTATT
GATGGTTGAATCAGAAGCTCAAGAATTGCCTGAAGACATCATGTTGGGTGCCGTTATGTTTGGACACAAAGGTCTTCAAC
CTATTCTTGATGCTATTATCAAACTTGCCGAAGTTGCTGCAAAAGATCCACGCGATTTTGTTCCCGAAGATCTCTCTGAT
CTCGAAACAGCTATGCTGGAAATGGCCGAAAAGGATATACGAAAAGCCTATACAATTACCGATAAACAGGAACGTTACGC
TGCACTTGATGCACTTAAAACAGAAATCATCAATAAATTTATGCCAGAAACAGAAGAAGACTGTAAATTTAGTGTAGATC
AAATTGCAACCGTTTTCAAACAGTTGCAAGCAAAAATTGTTCGCTCAAATATTCTTGATACGAAAAAGCGTATTGATGGA
CGCGACCTTTCAACAGTACGTCCCATCCAATCGGAGGTTGGTATTTTACCCCGAACACACGGATCGGCACTCTTTACCCG
TGGAGAAACACAAGCAATTGTCGTTGCAACACTAGGAACCGGTGAAGATGAACAATATATCGATTCTTTAACGGGTATGT
ATAAGGAAACATTCCTCCTTCATTATAATTTTCCACCATTTTCAGTGGGTGAAACAGGACGTCTTGGCTCTCCTGGTCGC
CGCGAAATCGGGCATGGTAAACTGGCGTGGCGTGCCATTCATCCTATGTTGCCAAGTAAGGAATCCTTTCCTTACACCAT
TCGTGCTGTTTCAGAAATCACTGAATCCAATGGATCTTCTTCCATGGCAACAGTTTGCGGAACTTCCCTTGCTCTCATGG
ATGCCGGTGTTCCTCTGGCACGTCCCGTTGCAGGTATTGCCATGGGCTTGATTAAAGAAGGTGAACGTTTTGCTGTGCTC
TCTGATATCTTAGGAGATGAAGATCATCTTGGAGACATGGATTTTAAAGTAGCCGGAACAGCAAATGGTATTACCGCCTT
GCAAATGGATATCAAAATTGATGGTATTACCGAAGAGATTATGAAAATCGCGCTTGAACAAGCCAAAGGTGGTCGAATCC
ATATCCTTAATGAAATGGCCAAAGCTTTAACCAGTGCACGTGCTGAACTCAGTGAATTTTCTCCACGCATCGAAGTTATG
AACATTGCTGTTGATAAAATTCGCGATGTTATCGGTACTGGCGGTAAAGTTATTCGAGAAATCGTTGAACAAACTGGAGC
AAAAATCAATATCGAAGATGATGGAACCATTAGAATTGCTTCTGCCGATGCCAAAACTATTGAAGCAGCAAAACGCTGGA
TTCATTCGATTGTTGATGAACCTGAAGTTGGCGTTATTTATCAAGGAACAGTTGTAAAAACTGCAGAATTCGGTGCATTT
GTAAACTTTTTTGGCTCACGTGATGGGCTTGTGCATATCTCTCAACTTACAACAGAACGTGTCACAAAAACAACAGATGT
TGTTAAAGAAGGCGATAAAGTTTGGGTTAAATTAATGGGTTTTGATGAGCGTGGTAAAGTTCGCTTATCAATGAAAATCG
TCGACCAAAAGACCGGTAAAGAAATTATTGGTGAGGATTCGATAAAAGCAGAACAAGAAAAATACACAGAGGAAACACAT
AAATCTGAAAACAAGCGCCGTCGTAAGAAAAAAGAAGAGTAA

Upstream 100 bases:

>100_bases
ACAGAAGTGTCTTGTTATCCTGCCCATAGCTTTAAAATATGCTACTCAAAACCAGAATGTGACCACTAAAAATGTGAACA
CTGGTCATTAAGGATAAAAA

Downstream 100 bases:

>100_bases
ACTTCTCCTTTTTTCATGAAATTAAATGCCATCCCAAATTGGAATGGCATTTTTCTCTAAAAGAGCCACATGTGTTATTA
TTTTTACCTTTTGAAAAGTA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 733; Mature: 733

Protein sequence:

>733_residues
MFKTHKIEIEWAGRPLTLETGKIARQADGAVIATYGETIVLATVVSAKSPKPDQDFFPLTVNYQEKSYAVGRIPGGYLKR
ESRPSENETLISRLIDRPIRPLFADGYKNDTQVIVSVIQHDLENNPDILAMIASSAALTLSGVPFMGPIAGARVGYCNGQ
YILNPHIDEMSESKLDLVVAGTENAVLMVESEAQELPEDIMLGAVMFGHKGLQPILDAIIKLAEVAAKDPRDFVPEDLSD
LETAMLEMAEKDIRKAYTITDKQERYAALDALKTEIINKFMPETEEDCKFSVDQIATVFKQLQAKIVRSNILDTKKRIDG
RDLSTVRPIQSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQYIDSLTGMYKETFLLHYNFPPFSVGETGRLGSPGR
REIGHGKLAWRAIHPMLPSKESFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPLARPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTANGITALQMDIKIDGITEEIMKIALEQAKGGRIHILNEMAKALTSARAELSEFSPRIEVM
NIAVDKIRDVIGTGGKVIREIVEQTGAKINIEDDGTIRIASADAKTIEAAKRWIHSIVDEPEVGVIYQGTVVKTAEFGAF
VNFFGSRDGLVHISQLTTERVTKTTDVVKEGDKVWVKLMGFDERGKVRLSMKIVDQKTGKEIIGEDSIKAEQEKYTEETH
KSENKRRRKKKEE

Sequences:

>Translated_733_residues
MFKTHKIEIEWAGRPLTLETGKIARQADGAVIATYGETIVLATVVSAKSPKPDQDFFPLTVNYQEKSYAVGRIPGGYLKR
ESRPSENETLISRLIDRPIRPLFADGYKNDTQVIVSVIQHDLENNPDILAMIASSAALTLSGVPFMGPIAGARVGYCNGQ
YILNPHIDEMSESKLDLVVAGTENAVLMVESEAQELPEDIMLGAVMFGHKGLQPILDAIIKLAEVAAKDPRDFVPEDLSD
LETAMLEMAEKDIRKAYTITDKQERYAALDALKTEIINKFMPETEEDCKFSVDQIATVFKQLQAKIVRSNILDTKKRIDG
RDLSTVRPIQSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQYIDSLTGMYKETFLLHYNFPPFSVGETGRLGSPGR
REIGHGKLAWRAIHPMLPSKESFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPLARPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTANGITALQMDIKIDGITEEIMKIALEQAKGGRIHILNEMAKALTSARAELSEFSPRIEVM
NIAVDKIRDVIGTGGKVIREIVEQTGAKINIEDDGTIRIASADAKTIEAAKRWIHSIVDEPEVGVIYQGTVVKTAEFGAF
VNFFGSRDGLVHISQLTTERVTKTTDVVKEGDKVWVKLMGFDERGKVRLSMKIVDQKTGKEIIGEDSIKAEQEKYTEETH
KSENKRRRKKKEE
>Mature_733_residues
MFKTHKIEIEWAGRPLTLETGKIARQADGAVIATYGETIVLATVVSAKSPKPDQDFFPLTVNYQEKSYAVGRIPGGYLKR
ESRPSENETLISRLIDRPIRPLFADGYKNDTQVIVSVIQHDLENNPDILAMIASSAALTLSGVPFMGPIAGARVGYCNGQ
YILNPHIDEMSESKLDLVVAGTENAVLMVESEAQELPEDIMLGAVMFGHKGLQPILDAIIKLAEVAAKDPRDFVPEDLSD
LETAMLEMAEKDIRKAYTITDKQERYAALDALKTEIINKFMPETEEDCKFSVDQIATVFKQLQAKIVRSNILDTKKRIDG
RDLSTVRPIQSEVGILPRTHGSALFTRGETQAIVVATLGTGEDEQYIDSLTGMYKETFLLHYNFPPFSVGETGRLGSPGR
REIGHGKLAWRAIHPMLPSKESFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPLARPVAGIAMGLIKEGERFAVL
SDILGDEDHLGDMDFKVAGTANGITALQMDIKIDGITEEIMKIALEQAKGGRIHILNEMAKALTSARAELSEFSPRIEVM
NIAVDKIRDVIGTGGKVIREIVEQTGAKINIEDDGTIRIASADAKTIEAAKRWIHSIVDEPEVGVIYQGTVVKTAEFGAF
VNFFGSRDGLVHISQLTTERVTKTTDVVKEGDKVWVKLMGFDERGKVRLSMKIVDQKTGKEIIGEDSIKAEQEKYTEETH
KSENKRRRKKKEE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=720, Percent_Identity=36.8055555555556, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI21361576, Length=98, Percent_Identity=41.8367346938776, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI4826690, Length=100, Percent_Identity=45, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI145693187, Length=686, Percent_Identity=56.8513119533528, Blast_Score=790, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=716, Percent_Identity=34.3575418994413, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535281, Length=84, Percent_Identity=47.6190476190476, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320850, Length=136, Percent_Identity=34.5588235294118, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=36.4902506963788, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=36.4902506963788, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=36.4902506963788, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=662, Percent_Identity=36.1027190332326, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=48.3516483516484, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_BARHE (Q6G5F8)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_033063.1
- HSSP:   P05055
- ProteinModelPortal:   Q6G5F8
- SMR:   Q6G5F8
- GeneID:   2865516
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH02100
- NMPDR:   fig|283166.1.peg.191
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q6G5F8
- ProtClustDB:   PRK11824
- BioCyc:   BHEN283166:BH02100-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 80653; Mature: 80653

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MFKTHKIEIEWAGRPLTLETGKIARQADGAVIATYGETIVLATVVSAKSPKPDQDFFPLT
CCCCEEEEEEECCCEEEEECCCHHHHCCCCEEEECCCEEEEEEEHHCCCCCCCCCCEEEE
VNYQEKSYAVGRIPGGYLKRESRPSENETLISRLIDRPIRPLFADGYKNDTQVIVSVIQH
EEECCCCEEECCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHH
DLENNPDILAMIASSAALTLSGVPFMGPIAGARVGYCNGQYILNPHIDEMSESKLDLVVA
HHCCCCCEEEEEECCCEEEECCCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCEEEEEE
GTENAVLMVESEAQELPEDIMLGAVMFGHKGLQPILDAIIKLAEVAAKDPRDFVPEDLSD
CCCCEEEEEECCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LETAMLEMAEKDIRKAYTITDKQERYAALDALKTEIINKFMPETEEDCKFSVDQIATVFK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHH
QLQAKIVRSNILDTKKRIDGRDLSTVRPIQSEVGILPRTHGSALFTRGETQAIVVATLGT
HHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHCCCCCCCCCCEEEECCCCCEEEEEEECC
GEDEQYIDSLTGMYKETFLLHYNFPPFSVGETGRLGSPGRREIGHGKLAWRAIHPMLPSK
CCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
ESFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPLARPVAGIAMGLIKEGERFAVL
CCCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHH
SDILGDEDHLGDMDFKVAGTANGITALQMDIKIDGITEEIMKIALEQAKGGRIHILNEMA
HHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHH
KALTSARAELSEFSPRIEVMNIAVDKIRDVIGTGGKVIREIVEQTGAKINIEDDGTIRIA
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEE
SADAKTIEAAKRWIHSIVDEPEVGVIYQGTVVKTAEFGAFVNFFGSRDGLVHISQLTTER
CCCHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHHCCCCCEEEHHHHHHHH
VTKTTDVVKEGDKVWVKLMGFDERGKVRLSMKIVDQKTGKEIIGEDSIKAEQEKYTEETH
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEECCCCCCHHHCCCCCHHHHHHHHHHHH
KSENKRRRKKKEE
HHHHHHHHHCCCC
>Mature Secondary Structure
MFKTHKIEIEWAGRPLTLETGKIARQADGAVIATYGETIVLATVVSAKSPKPDQDFFPLT
CCCCEEEEEEECCCEEEEECCCHHHHCCCCEEEECCCEEEEEEEHHCCCCCCCCCCEEEE
VNYQEKSYAVGRIPGGYLKRESRPSENETLISRLIDRPIRPLFADGYKNDTQVIVSVIQH
EEECCCCEEECCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHH
DLENNPDILAMIASSAALTLSGVPFMGPIAGARVGYCNGQYILNPHIDEMSESKLDLVVA
HHCCCCCEEEEEECCCEEEECCCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCEEEEEE
GTENAVLMVESEAQELPEDIMLGAVMFGHKGLQPILDAIIKLAEVAAKDPRDFVPEDLSD
CCCCEEEEEECCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
LETAMLEMAEKDIRKAYTITDKQERYAALDALKTEIINKFMPETEEDCKFSVDQIATVFK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHH
QLQAKIVRSNILDTKKRIDGRDLSTVRPIQSEVGILPRTHGSALFTRGETQAIVVATLGT
HHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHCCCCCCCCCCEEEECCCCCEEEEEEECC
GEDEQYIDSLTGMYKETFLLHYNFPPFSVGETGRLGSPGRREIGHGKLAWRAIHPMLPSK
CCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
ESFPYTIRAVSEITESNGSSSMATVCGTSLALMDAGVPLARPVAGIAMGLIKEGERFAVL
CCCCCHHHHHHHHHHCCCCCCHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHH
SDILGDEDHLGDMDFKVAGTANGITALQMDIKIDGITEEIMKIALEQAKGGRIHILNEMA
HHHCCCCCCCCCCCEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHH
KALTSARAELSEFSPRIEVMNIAVDKIRDVIGTGGKVIREIVEQTGAKINIEDDGTIRIA
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEE
SADAKTIEAAKRWIHSIVDEPEVGVIYQGTVVKTAEFGAFVNFFGSRDGLVHISQLTTER
CCCHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHHCCCCCEEEHHHHHHHH
VTKTTDVVKEGDKVWVKLMGFDERGKVRLSMKIVDQKTGKEIIGEDSIKAEQEKYTEETH
HHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEEECCCCCCHHHCCCCCHHHHHHHHHHHH
KSENKRRRKKKEE
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA