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The map label for this gene is mltB [H]

Identifier: 49474987

GI number: 49474987

Start: 238803

End: 240059

Strand: Reverse

Name: mltB [H]

Synonym: BH01740

Alternate gene names: 49474987

Gene position: 240059-238803 (Counterclockwise)

Preceding gene: 49474990

Following gene: 49474986

Centisome position: 12.43

GC content: 39.62

Gene sequence:

>1257_bases
ATGCAAAAAACAGAATCTAAGACCTTCTTTTATTCAGAGAAACCGATAAATTCTAGAGCTCTTATCATTGGTTTAGCGGT
TCTTTTCTCTGTTTTTTTAATGTTTTTTGCACAACTTTCATACGCTAATGTTGGTTTTCAACAGTGGATTAAAGAATTTA
AAAAAGTAGCTCTCACCAATAATATTTTATCCTCTACATTTGATATGGCTTTTAAAGGAATCAACGCAATTGATCCAGAA
GTTTTAAAAAAAGCCGCATACCAACCGGAATTTGTTGATCCTTCATGGAACTATTTCGACAATCGTGTTCATGACATTGC
AATTATGGAAGGACAGAGCCAAACTAAAAAATGGCAAAAGTGGCTTTCTCAAATTGAAAAACGTTTTGGTGTTGATCGTA
ATATCCTCCTGGCGATTTGGTCAATGGAAAGCAATTATGGAAAAGTTTTAGCAAATAAAGATGTGATGCGTGATACCATT
CGCTCATTAGCAACCTTAGCCTATGCTGATCAAAAACGCGCAAAATATGCACAAGCGCAATTGATTGCTGCCATGACAAT
TCTCCAAAACGGTGAAATTACACGTGCACAACTTACTGGCTCTTGGGCTGGTGCAATGGGACATACACAATTTATTCCAA
GCAGTTATCTCGCTTATGCCATTGACATGGATGGGGATGGACGACGTGATATTTGGACTTCTGTTCCAGATGCCCTCGCA
ACCGCTGCTAATCTTCTCTACATGAATGGTTGGCAGTCTAAACTTCCTTGGGGAGTGGAAGTTAAATTGCCGCAAGGAAA
AAATCTTCCCGAGGATTGGCATTCATTTGAACAATGGGCAAAGCTAGGTGTAAAACAGGCGAATGGAAAACATTTGCCTT
CATTATCTGAACGAGCAATATTGAAATACCCAGATGGGCCAGAAGGACCTATATTTTTAGTAACAAAAAATTTCTTTGTT
CTTAAACGTTATAATAATGCAGATCGCTACGCTTTCGCTGTTGCACTTCTTGCTAACCGCATTGCTGGACATTCTGGATT
GGTTCAAGATTGGCAGCGACCATTTCCGCCTCTTACTTTTTACGAACGCAAAGAACTGCAATCTCGCTTAGCAGCACTTG
GCTATTATAAAGGAGAAATGGATGGCATAATCGGTACAGCCTCTCGAAAAGCACTTAAAGCTTTTCAGCTGCGCCACGGC
TTAACAGCAAATGGATATCCAACCCCTGAAGTTCTCTCCCTTCTTCGAAAACAATAA

Upstream 100 bases:

>100_bases
TCAGCCTTTTTTATTTACTTGATAATATGATTAATAAAACCAAATACATAAATGAAATCCTTGCACATAAAAATATTTGA
ATATTGAAAGGAATTTTAAA

Downstream 100 bases:

>100_bases
TCTGGAGTAAAGTGTTTGTTCCTTTTCCGTCCGATACTCCTGATTTTTCTGCTTTTCTCTCTCCTTGTCGTGAGTGTCAT
ACAACCATCTCCAAGTCAAG

Product: peptidoglycan-binding protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MQKTESKTFFYSEKPINSRALIIGLAVLFSVFLMFFAQLSYANVGFQQWIKEFKKVALTNNILSSTFDMAFKGINAIDPE
VLKKAAYQPEFVDPSWNYFDNRVHDIAIMEGQSQTKKWQKWLSQIEKRFGVDRNILLAIWSMESNYGKVLANKDVMRDTI
RSLATLAYADQKRAKYAQAQLIAAMTILQNGEITRAQLTGSWAGAMGHTQFIPSSYLAYAIDMDGDGRRDIWTSVPDALA
TAANLLYMNGWQSKLPWGVEVKLPQGKNLPEDWHSFEQWAKLGVKQANGKHLPSLSERAILKYPDGPEGPIFLVTKNFFV
LKRYNNADRYAFAVALLANRIAGHSGLVQDWQRPFPPLTFYERKELQSRLAALGYYKGEMDGIIGTASRKALKAFQLRHG
LTANGYPTPEVLSLLRKQ

Sequences:

>Translated_418_residues
MQKTESKTFFYSEKPINSRALIIGLAVLFSVFLMFFAQLSYANVGFQQWIKEFKKVALTNNILSSTFDMAFKGINAIDPE
VLKKAAYQPEFVDPSWNYFDNRVHDIAIMEGQSQTKKWQKWLSQIEKRFGVDRNILLAIWSMESNYGKVLANKDVMRDTI
RSLATLAYADQKRAKYAQAQLIAAMTILQNGEITRAQLTGSWAGAMGHTQFIPSSYLAYAIDMDGDGRRDIWTSVPDALA
TAANLLYMNGWQSKLPWGVEVKLPQGKNLPEDWHSFEQWAKLGVKQANGKHLPSLSERAILKYPDGPEGPIFLVTKNFFV
LKRYNNADRYAFAVALLANRIAGHSGLVQDWQRPFPPLTFYERKELQSRLAALGYYKGEMDGIIGTASRKALKAFQLRHG
LTANGYPTPEVLSLLRKQ
>Mature_418_residues
MQKTESKTFFYSEKPINSRALIIGLAVLFSVFLMFFAQLSYANVGFQQWIKEFKKVALTNNILSSTFDMAFKGINAIDPE
VLKKAAYQPEFVDPSWNYFDNRVHDIAIMEGQSQTKKWQKWLSQIEKRFGVDRNILLAIWSMESNYGKVLANKDVMRDTI
RSLATLAYADQKRAKYAQAQLIAAMTILQNGEITRAQLTGSWAGAMGHTQFIPSSYLAYAIDMDGDGRRDIWTSVPDALA
TAANLLYMNGWQSKLPWGVEVKLPQGKNLPEDWHSFEQWAKLGVKQANGKHLPSLSERAILKYPDGPEGPIFLVTKNFFV
LKRYNNADRYAFAVALLANRIAGHSGLVQDWQRPFPPLTFYERKELQSRLAALGYYKGEMDGIIGTASRKALKAFQLRHG
LTANGYPTPEVLSLLRKQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=235, Percent_Identity=29.3617021276596, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 47374; Mature: 47374

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTESKTFFYSEKPINSRALIIGLAVLFSVFLMFFAQLSYANVGFQQWIKEFKKVALTN
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NILSSTFDMAFKGINAIDPEVLKKAAYQPEFVDPSWNYFDNRVHDIAIMEGQSQTKKWQK
HHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHCCCEEEEEEECCCHHHHHHHH
WLSQIEKRFGVDRNILLAIWSMESNYGKVLANKDVMRDTIRSLATLAYADQKRAKYAQAQ
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAAMTILQNGEITRAQLTGSWAGAMGHTQFIPSSYLAYAIDMDGDGRRDIWTSVPDALA
HHHHHHHHCCCCEEEEEECCCHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCHHHHH
TAANLLYMNGWQSKLPWGVEVKLPQGKNLPEDWHSFEQWAKLGVKQANGKHLPSLSERAI
HHHHHHEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LKYPDGPEGPIFLVTKNFFVLKRYNNADRYAFAVALLANRIAGHSGLVQDWQRPFPPLTF
EECCCCCCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCH
YERKELQSRLAALGYYKGEMDGIIGTASRKALKAFQLRHGLTANGYPTPEVLSLLRKQ
HHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MQKTESKTFFYSEKPINSRALIIGLAVLFSVFLMFFAQLSYANVGFQQWIKEFKKVALTN
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NILSSTFDMAFKGINAIDPEVLKKAAYQPEFVDPSWNYFDNRVHDIAIMEGQSQTKKWQK
HHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCHHCCCEEEEEEECCCHHHHHHHH
WLSQIEKRFGVDRNILLAIWSMESNYGKVLANKDVMRDTIRSLATLAYADQKRAKYAQAQ
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAAMTILQNGEITRAQLTGSWAGAMGHTQFIPSSYLAYAIDMDGDGRRDIWTSVPDALA
HHHHHHHHCCCCEEEEEECCCHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHCCHHHHH
TAANLLYMNGWQSKLPWGVEVKLPQGKNLPEDWHSFEQWAKLGVKQANGKHLPSLSERAI
HHHHHHEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LKYPDGPEGPIFLVTKNFFVLKRYNNADRYAFAVALLANRIAGHSGLVQDWQRPFPPLTF
EECCCCCCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCH
YERKELQSRLAALGYYKGEMDGIIGTASRKALKAFQLRHGLTANGYPTPEVLSLLRKQ
HHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]