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The map label for this gene is truA

Identifier: 49474896

GI number: 49474896

Start: 100329

End: 101072

Strand: Reverse

Name: truA

Synonym: BH00740

Alternate gene names: 49474896

Gene position: 101072-100329 (Counterclockwise)

Preceding gene: 49474897

Following gene: 49474894

Centisome position: 5.23

GC content: 44.22

Gene sequence:

>744_bases
ATGCCACGTTTTAAACTCACTCTTGAATATGATGGCTCAAATTATGCCGGCTGGCAGCGCCAAGCAGAACTTCGTACTAT
ACAGAGTGCTCTTGAACAGGCACTTTTTCATTTTAGTGGGCAACAATTGACCATTACGACAGCTGGTAGAACTGATGCTG
GTGTACATGCTACCGGACAAGTTGCACATGTTGATTTTGAAAAAAATTGGCGTACACATACTGTACGCGATGCGCTTAAT
GCGCATTTGCAAAAACAGGGAGACAATATTGCAATCTTGCATGTACAAAACGTCCCCGATAGTTTTGATGCACGATTTTC
CGCAATCAAACGCCATTATCTTTTTAAAATTCTCAATCGCCGCTCTCCACCAGCCCTAAACACTAAACGCGTATGGTGGA
TACCAAAACCCCTTAATGCGCAAGCCATGCATGAAGCTGCCCAAAAACTTGTAGGGAAACATGATTTTACCACCTTTCGT
TCAGCACATTGTCAAGCAAAAAGTCCTATTCGCACCCTTGAACGTCTCGATGTTCAAAGAGAGGGAGAAGAGATCTTCCT
CTATGCACAAGCGCGCTCCTTTCTTCATCATCAAATTCGTTCATTTGCAGGAAGCCTCATGGAAGTTGGTATTGGACGCT
GGACAACACAAGATCTTGAAGCAGCTCTTCATGCCAAAGATCGCACACGTTGTGGTATGGTCGCGCCACCTTCAGGACTT
TATTTGACCAAGGTGGAATATTAA

Upstream 100 bases:

>100_bases
CAAGGGTGTCTTGAAGTAACCTATTTGCAAAGATCTGGTGGTAAAGTTCTTGAGTGTGCGACCTTTTTGCAAGGTGCTCA
TATTTCTGCTGTTTTTTAAT

Downstream 100 bases:

>100_bases
AAACTGTTTAAAAATTACATCCTTCAAAACGATTAAGGAAGCTTATAAAAGCAACGAATTTCTTCAAAAGCTTGTGCTGC
TGCGTCAACAAATTTCACCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MPRFKLTLEYDGSNYAGWQRQAELRTIQSALEQALFHFSGQQLTITTAGRTDAGVHATGQVAHVDFEKNWRTHTVRDALN
AHLQKQGDNIAILHVQNVPDSFDARFSAIKRHYLFKILNRRSPPALNTKRVWWIPKPLNAQAMHEAAQKLVGKHDFTTFR
SAHCQAKSPIRTLERLDVQREGEEIFLYAQARSFLHHQIRSFAGSLMEVGIGRWTTQDLEAALHAKDRTRCGMVAPPSGL
YLTKVEY

Sequences:

>Translated_247_residues
MPRFKLTLEYDGSNYAGWQRQAELRTIQSALEQALFHFSGQQLTITTAGRTDAGVHATGQVAHVDFEKNWRTHTVRDALN
AHLQKQGDNIAILHVQNVPDSFDARFSAIKRHYLFKILNRRSPPALNTKRVWWIPKPLNAQAMHEAAQKLVGKHDFTTFR
SAHCQAKSPIRTLERLDVQREGEEIFLYAQARSFLHHQIRSFAGSLMEVGIGRWTTQDLEAALHAKDRTRCGMVAPPSGL
YLTKVEY
>Mature_246_residues
PRFKLTLEYDGSNYAGWQRQAELRTIQSALEQALFHFSGQQLTITTAGRTDAGVHATGQVAHVDFEKNWRTHTVRDALNA
HLQKQGDNIAILHVQNVPDSFDARFSAIKRHYLFKILNRRSPPALNTKRVWWIPKPLNAQAMHEAAQKLVGKHDFTTFRS
AHCQAKSPIRTLERLDVQREGEEIFLYAQARSFLHHQIRSFAGSLMEVGIGRWTTQDLEAALHAKDRTRCGMVAPPSGLY
LTKVEY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=280, Percent_Identity=31.7857142857143, Blast_Score=111, Evalue=7e-25,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=43.6734693877551, Blast_Score=199, Evalue=2e-52,
Organism=Drosophila melanogaster, GI116007752, Length=302, Percent_Identity=28.476821192053, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_BARHE (Q6G547)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_032937.1
- ProteinModelPortal:   Q6G547
- SMR:   Q6G547
- GeneID:   2865243
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH00740
- NMPDR:   fig|283166.1.peg.65
- HOGENOM:   HBG621020
- OMA:   AIKRHYL
- PhylomeDB:   Q6G547
- ProtClustDB:   PRK00021
- BioCyc:   BHEN283166:BH00740-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28169; Mature: 28038

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFKLTLEYDGSNYAGWQRQAELRTIQSALEQALFHFSGQQLTITTAGRTDAGVHATGQ
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
VAHVDFEKNWRTHTVRDALNAHLQKQGDNIAILHVQNVPDSFDARFSAIKRHYLFKILNR
EEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
RSPPALNTKRVWWIPKPLNAQAMHEAAQKLVGKHDFTTFRSAHCQAKSPIRTLERLDVQR
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHCC
EGEEIFLYAQARSFLHHQIRSFAGSLMEVGIGRWTTQDLEAALHAKDRTRCGMVAPPSGL
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCE
YLTKVEY
EEEEECC
>Mature Secondary Structure 
PRFKLTLEYDGSNYAGWQRQAELRTIQSALEQALFHFSGQQLTITTAGRTDAGVHATGQ
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
VAHVDFEKNWRTHTVRDALNAHLQKQGDNIAILHVQNVPDSFDARFSAIKRHYLFKILNR
EEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
RSPPALNTKRVWWIPKPLNAQAMHEAAQKLVGKHDFTTFRSAHCQAKSPIRTLERLDVQR
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHCC
EGEEIFLYAQARSFLHHQIRSFAGSLMEVGIGRWTTQDLEAALHAKDRTRCGMVAPPSGL
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCE
YLTKVEY
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA