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The map label for this gene is aarF [H]

Identifier: 49474865

GI number: 49474865

Start: 50964

End: 52550

Strand: Reverse

Name: aarF [H]

Synonym: BH00390

Alternate gene names: 49474865

Gene position: 52550-50964 (Counterclockwise)

Preceding gene: 49474866

Following gene: 49474864

Centisome position: 2.72

GC content: 40.14

Gene sequence:

>1587_bases
ATGGTGCAAATTTCCACTTACTTTCAATTAATACGTACTGGATGGGTTTTAGCACGTGAAGGCGTTTTGAGTGCTTTGCC
TCACGATGATCTTCAAGGATTTCCAGCATTATGCCACCGTATCGCAAGTATGTTAGCGCGGCGTAAAACGAAAAAAAAGC
GACGATCTGAAAAACTTTCCTATGCCATCAATAGGCTCGGCCCTTCTTACATAAAACTTGGTCAATTTCTTGCTACCAGA
CCCGATATTGTAGGACGTGATATTGCGGAAGATTTAGCACAACTACAAGATCGTGTTCAAACATTTTCCTGTACGGCTGC
CATTGATCAAATTGAAAGTTCGCTTGGTCGCTCTATCAATGATTTATTCGCAAACTTTTATCCCCCTATTGCAGCCGCTT
CTATTGCTCAAGTTCATCCAGCTGAATACTACGATGAAGTAGGCCATAAAAAAAAATGTGCTGTTAAGGTCATTCGCCCT
AATATCAGAACGCGTTTTGCAAAAGACCTTAGAAGCTTTTACCTTATTGCCCATTTACAGGAACGTTATATACCAGCTGC
TCGACGTCTGCGTCCTGTTTGTGTCGTTGATACTTTGGCACAAACCACACGAATTGAAATGGACTTGCGATTAGAAGCAG
CGGCCATATCAGAAATGGCTGAAAATATTCAACATGATACAGGCTTTCGGGTACCAAAAATTGATTGGGAACGAACAGGA
CGTAATGTTCTCACAATGGAATGGATTGATGGTATAAGAATATCCGAAATTTCTGCTCTCAAAGAAGCAGGGTTTGATTT
GCAAGCACTTGCAATTACACTTATTCAATCTTTCCTTCGTCATACATTACGTGATGGTTTTTTTCACGCTGATATGCATC
CTGGCAATTTATTTGTAGACCCACAAGGATGTATTGTTGCTGTCGATTTAGGAATTACAGGACGACTTGGTAAAAAAGAA
AAGCATTTCCTTGCTGAAATTCTCTATGGCTTTATTACTCGCGATTACCATCGGGTAGCACGTGCTCATTTTGAAGCAGG
ATATGTACCTCCACACCACAATATTGAGAGTTTTGCTCAAGCCAATCGCGCAATTGGTGAACCAATTCACGGACAATCAG
CGCAAAGCATTTCTATGGCTAAGTTACTTACACTGTTATTTGAAGTCACTGAATTATTTGACATGCAAACGCGACCTGAA
CTTTTATTACTCCAAAAAACTATGGTTGTCGTGGAAGGTGTTGCTCGCAACTTAGATCCCAATTTTAATATGTGGAAAGC
TTCTGAACCCGTTGTTAAAGAATGGATTAGCAAAAATTTAGGGCCCGTGGGAATAGCAAAAGACTTTAGTGAAGGAGCGC
AGGCACTTTTTTCGCTAGTGCGTAACACACCACAGTTGATACAAAATTTCCAACGTATGGAAGAAGACCTCAATCAAATG
ATAAAAACAGGATTTAATCTTTCTCCCACTACCCTCAAAAAACTTGCGGTCGAACAAAGTCGCTCTAATCGTTATGGCCG
TTATAGCCTTTTCATCATTGCGCTTTGTTGTATTTGTCTGACTTTTTACCTTTTTCATTTTTTTTAA

Upstream 100 bases:

>100_bases
TTTTGCCCATATGATCAATCATGTAGGTTTTTCGCGAGTATCATACCGCAACCTCACGGGTGCAATCGCAGCGCTGCATT
CAGCCTGGAAGATTTAAAAA

Downstream 100 bases:

>100_bases
TCTGTTGAATATACTCAAAAAATATTTATAATGAAATCATTGCAATCATTTTGTTTTTTGAGTGTATTTTATGGCCAGTC
TTACCATCCGCAATCTTCCA

Product: ubiquinone biosynthesis protein aarF

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MVQISTYFQLIRTGWVLAREGVLSALPHDDLQGFPALCHRIASMLARRKTKKKRRSEKLSYAINRLGPSYIKLGQFLATR
PDIVGRDIAEDLAQLQDRVQTFSCTAAIDQIESSLGRSINDLFANFYPPIAAASIAQVHPAEYYDEVGHKKKCAVKVIRP
NIRTRFAKDLRSFYLIAHLQERYIPAARRLRPVCVVDTLAQTTRIEMDLRLEAAAISEMAENIQHDTGFRVPKIDWERTG
RNVLTMEWIDGIRISEISALKEAGFDLQALAITLIQSFLRHTLRDGFFHADMHPGNLFVDPQGCIVAVDLGITGRLGKKE
KHFLAEILYGFITRDYHRVARAHFEAGYVPPHHNIESFAQANRAIGEPIHGQSAQSISMAKLLTLLFEVTELFDMQTRPE
LLLLQKTMVVVEGVARNLDPNFNMWKASEPVVKEWISKNLGPVGIAKDFSEGAQALFSLVRNTPQLIQNFQRMEEDLNQM
IKTGFNLSPTTLKKLAVEQSRSNRYGRYSLFIIALCCICLTFYLFHFF

Sequences:

>Translated_528_residues
MVQISTYFQLIRTGWVLAREGVLSALPHDDLQGFPALCHRIASMLARRKTKKKRRSEKLSYAINRLGPSYIKLGQFLATR
PDIVGRDIAEDLAQLQDRVQTFSCTAAIDQIESSLGRSINDLFANFYPPIAAASIAQVHPAEYYDEVGHKKKCAVKVIRP
NIRTRFAKDLRSFYLIAHLQERYIPAARRLRPVCVVDTLAQTTRIEMDLRLEAAAISEMAENIQHDTGFRVPKIDWERTG
RNVLTMEWIDGIRISEISALKEAGFDLQALAITLIQSFLRHTLRDGFFHADMHPGNLFVDPQGCIVAVDLGITGRLGKKE
KHFLAEILYGFITRDYHRVARAHFEAGYVPPHHNIESFAQANRAIGEPIHGQSAQSISMAKLLTLLFEVTELFDMQTRPE
LLLLQKTMVVVEGVARNLDPNFNMWKASEPVVKEWISKNLGPVGIAKDFSEGAQALFSLVRNTPQLIQNFQRMEEDLNQM
IKTGFNLSPTTLKKLAVEQSRSNRYGRYSLFIIALCCICLTFYLFHFF
>Mature_528_residues
MVQISTYFQLIRTGWVLAREGVLSALPHDDLQGFPALCHRIASMLARRKTKKKRRSEKLSYAINRLGPSYIKLGQFLATR
PDIVGRDIAEDLAQLQDRVQTFSCTAAIDQIESSLGRSINDLFANFYPPIAAASIAQVHPAEYYDEVGHKKKCAVKVIRP
NIRTRFAKDLRSFYLIAHLQERYIPAARRLRPVCVVDTLAQTTRIEMDLRLEAAAISEMAENIQHDTGFRVPKIDWERTG
RNVLTMEWIDGIRISEISALKEAGFDLQALAITLIQSFLRHTLRDGFFHADMHPGNLFVDPQGCIVAVDLGITGRLGKKE
KHFLAEILYGFITRDYHRVARAHFEAGYVPPHHNIESFAQANRAIGEPIHGQSAQSISMAKLLTLLFEVTELFDMQTRPE
LLLLQKTMVVVEGVARNLDPNFNMWKASEPVVKEWISKNLGPVGIAKDFSEGAQALFSLVRNTPQLIQNFQRMEEDLNQM
IKTGFNLSPTTLKKLAVEQSRSNRYGRYSLFIIALCCICLTFYLFHFF

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI41393593, Length=274, Percent_Identity=28.8321167883212, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI40254938, Length=342, Percent_Identity=25.7309941520468, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI217035081, Length=280, Percent_Identity=26.0714285714286, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI2367309, Length=500, Percent_Identity=35.4, Blast_Score=287, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17559152, Length=375, Percent_Identity=23.2, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325148, Length=438, Percent_Identity=23.5159817351598, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323282, Length=278, Percent_Identity=24.8201438848921, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24662575, Length=331, Percent_Identity=26.5861027190332, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI22024280, Length=281, Percent_Identity=24.5551601423488, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 59966; Mature: 59966

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQISTYFQLIRTGWVLAREGVLSALPHDDLQGFPALCHRIASMLARRKTKKKRRSEKLS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAINRLGPSYIKLGQFLATRPDIVGRDIAEDLAQLQDRVQTFSCTAAIDQIESSLGRSIN
HHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DLFANFYPPIAAASIAQVHPAEYYDEVGHKKKCAVKVIRPNIRTRFAKDLRSFYLIAHLQ
HHHHHHCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERYIPAARRLRPVCVVDTLAQTTRIEMDLRLEAAAISEMAENIQHDTGFRVPKIDWERTG
HHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
RNVLTMEWIDGIRISEISALKEAGFDLQALAITLIQSFLRHTLRDGFFHADMHPGNLFVD
CCEEEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEC
PQGCIVAVDLGITGRLGKKEKHFLAEILYGFITRDYHRVARAHFEAGYVPPHHNIESFAQ
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ANRAIGEPIHGQSAQSISMAKLLTLLFEVTELFDMQTRPELLLLQKTMVVVEGVARNLDP
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
NFNMWKASEPVVKEWISKNLGPVGIAKDFSEGAQALFSLVRNTPQLIQNFQRMEEDLNQM
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IKTGFNLSPTTLKKLAVEQSRSNRYGRYSLFIIALCCICLTFYLFHFF
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MVQISTYFQLIRTGWVLAREGVLSALPHDDLQGFPALCHRIASMLARRKTKKKRRSEKLS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAINRLGPSYIKLGQFLATRPDIVGRDIAEDLAQLQDRVQTFSCTAAIDQIESSLGRSIN
HHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
DLFANFYPPIAAASIAQVHPAEYYDEVGHKKKCAVKVIRPNIRTRFAKDLRSFYLIAHLQ
HHHHHHCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ERYIPAARRLRPVCVVDTLAQTTRIEMDLRLEAAAISEMAENIQHDTGFRVPKIDWERTG
HHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
RNVLTMEWIDGIRISEISALKEAGFDLQALAITLIQSFLRHTLRDGFFHADMHPGNLFVD
CCEEEEHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEC
PQGCIVAVDLGITGRLGKKEKHFLAEILYGFITRDYHRVARAHFEAGYVPPHHNIESFAQ
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ANRAIGEPIHGQSAQSISMAKLLTLLFEVTELFDMQTRPELLLLQKTMVVVEGVARNLDP
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
NFNMWKASEPVVKEWISKNLGPVGIAKDFSEGAQALFSLVRNTPQLIQNFQRMEEDLNQM
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IKTGFNLSPTTLKKLAVEQSRSNRYGRYSLFIIALCCICLTFYLFHFF
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA