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The map label for this gene is adaB [H]

Identifier: 49186587

GI number: 49186587

Start: 3542106

End: 3542636

Strand: Direct

Name: adaB [H]

Synonym: BAS3586

Alternate gene names: 49186587

Gene position: 3542106-3542636 (Clockwise)

Preceding gene: 49186586

Following gene: 49186588

Centisome position: 67.74

GC content: 33.71

Gene sequence:

>531_bases
ATGAATTCCTACAAAAATAAATTTATATATTGGACGCTACTTACACATGCAAATTGGAAGTTTCATATTGCTGCAACTGA
AAGTGGACTATGTTTCATTGGATCACAAGACGAACAATTTGAGGAATTAAATATATGGGCTAGAAAAAAACTGCCACAAT
ATATATTGATTCATAGTCCAGATTACTTGCAAGTATATACAAAAGAAATTATCGAGTATTTAAAAAACAAGAGAGAAACT
TTTACATTCCCTATCGATGCTTACGGAACTGCATTTCAATTATCTGTTTGGAATACGGTACGTGAAATTCCTTATGGGAA
AACATATTCTTATACAGAAATTGCAGATAGAATTCAAAAACCTACAGCGGTACGTGCCGTTGCTTCTGCTATTGCTGCCA
ACCCAATTCTCATTACGATTCCTTGCCATCGTGTTATTGGAAAGAATGGCAAACTAACTGGTTTTAGAGGGGGATTAGAA
ATGAAGAAAGAATTGCTTGTATTAGAAAAATTACAGGTGGAATTCATATGA

Upstream 100 bases:

>100_bases
ATAGAAAACCCAGAATATTTCGCAACTTTATTTAAAAAGAAAACTGGGTTTACCCCTACTGAATATCGAAAAAAGAATGA
AATGAAAGAGAGATACAATA

Downstream 100 bases:

>100_bases
CAGCCGAATATAGTAACGCATTAATTTTAACAGTTCCAACAGAATTTAGTTTTCAAGAAAATTTACGCTATCTATCCCGT
TCTAACAATGAATGTATGTT

Product: methylated-DNA--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MNSYKNKFIYWTLLTHANWKFHIAATESGLCFIGSQDEQFEELNIWARKKLPQYILIHSPDYLQVYTKEIIEYLKNKRET
FTFPIDAYGTAFQLSVWNTVREIPYGKTYSYTEIADRIQKPTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLE
MKKELLVLEKLQVEFI

Sequences:

>Translated_176_residues
MNSYKNKFIYWTLLTHANWKFHIAATESGLCFIGSQDEQFEELNIWARKKLPQYILIHSPDYLQVYTKEIIEYLKNKRET
FTFPIDAYGTAFQLSVWNTVREIPYGKTYSYTEIADRIQKPTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLE
MKKELLVLEKLQVEFI
>Mature_176_residues
MNSYKNKFIYWTLLTHANWKFHIAATESGLCFIGSQDEQFEELNIWARKKLPQYILIHSPDYLQVYTKEIIEYLKNKRET
FTFPIDAYGTAFQLSVWNTVREIPYGKTYSYTEIADRIQKPTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLE
MKKELLVLEKLQVEFI

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=112, Percent_Identity=29.4642857142857, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1788542, Length=150, Percent_Identity=41.3333333333333, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787596, Length=124, Percent_Identity=40.3225806451613, Blast_Score=96, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=86, Percent_Identity=47.6744186046512, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=86, Percent_Identity=47.6744186046512, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=43.3734939759036, Blast_Score=85, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17137554, Length=123, Percent_Identity=39.0243902439024, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20383; Mature: 20383

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSYKNKFIYWTLLTHANWKFHIAATESGLCFIGSQDEQFEELNIWARKKLPQYILIHSP
CCCCCCEEEEEEEEECCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECC
DYLQVYTKEIIEYLKNKRETFTFPIDAYGTAFQLSVWNTVREIPYGKTYSYTEIADRIQK
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHCC
PTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLEMKKELLVLEKLQVEFI
CHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MNSYKNKFIYWTLLTHANWKFHIAATESGLCFIGSQDEQFEELNIWARKKLPQYILIHSP
CCCCCCEEEEEEEEECCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECC
DYLQVYTKEIIEYLKNKRETFTFPIDAYGTAFQLSVWNTVREIPYGKTYSYTEIADRIQK
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHHCCCCCCCCHHHHHHHHCC
PTAVRAVASAIAANPILITIPCHRVIGKNGKLTGFRGGLEMKKELLVLEKLQVEFI
CHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]