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The map label for this gene is adaA [H]

Identifier: 49186586

GI number: 49186586

Start: 3541529

End: 3542125

Strand: Direct

Name: adaA [H]

Synonym: BAS3585

Alternate gene names: 49186586

Gene position: 3541529-3542125 (Clockwise)

Preceding gene: 49186575

Following gene: 49186587

Centisome position: 67.73

GC content: 34.84

Gene sequence:

>597_bases
TTGCACAATGAAGGAGTTACGTTAACAAATGAGCATTGGCAAGCAATTATTCATAATGATTCTTCCTATGATAGCAAATT
CTTTTATGCCGTGAAATCAACCGGAGTTTTTTGCCGACCGTCATGCAAATCAAGAATACCGAATAAAAATAATGTACGAA
TTTTTCATCATGCAGAGCAAGCATTAAGTGAAAACTTTCGCCCGTGCAAACGTTGTAAACCAAATGGGCTCACTTTACCT
AATGAAGAGTGGGTAGAACAAATTAAAGAGTATATCGAAAAACACTTTGATGAAGCATTAACTTTAGACATACTTGCGGA
AATGTGCCATGGTAGCCCCTTTCATTTACAACGCACTTTCAAAAAAATGACAGCAATAAGTCCGATAGAATATATACAGC
AGTTCAGAATTGTTAAAGCAGCAGAACACCTTTTACACACAAATCAACCCATTAAAGAAATTAGTACTGCCGTCGGGATA
GAAAACCCAGAATATTTCGCAACTTTATTTAAAAAGAAAACTGGGTTTACCCCTACTGAATATCGAAAAAAGAATGAAAT
GAAAGAGAGATACAATAATGAATTCCTACAAAAATAA

Upstream 100 bases:

>100_bases
AAAACAATCATATTTTCACCTTTGTAATCTTGCTCTTACATTTATAAATACATACATACGCACTTTATTCAGGTTATACT
AATTTCAGAGGTGATGTTCT

Downstream 100 bases:

>100_bases
ATTTATATATTGGACGCTACTTACACATGCAAATTGGAAGTTTCATATTGCTGCAACTGAAAGTGGACTATGTTTCATTG
GATCACAAGACGAACAATTT

Product: Ada regulatory protein/6-O-methylguanine-DNA methyltransferase

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQALSENFRPCKRCKPNGLTLP
NEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTFKKMTAISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGI
ENPEYFATLFKKKTGFTPTEYRKKNEMKERYNNEFLQK

Sequences:

>Translated_198_residues
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQALSENFRPCKRCKPNGLTLP
NEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTFKKMTAISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGI
ENPEYFATLFKKKTGFTPTEYRKKNEMKERYNNEFLQK
>Mature_198_residues
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQALSENFRPCKRCKPNGLTLP
NEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTFKKMTAISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGI
ENPEYFATLFKKKTGFTPTEYRKKNEMKERYNNEFLQK

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=184, Percent_Identity=31.5217391304348, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790497, Length=106, Percent_Identity=28.3018867924528, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1790391, Length=99, Percent_Identity=34.3434343434343, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787987, Length=121, Percent_Identity=31.404958677686, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63

Molecular weight: Translated: 23281; Mature: 23281

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQ
CCCCCCEEEHHHHEEEEECCCCCCCCEEEEEECCCEEECCCHHHCCCCCCCEEEEHHHHH
ALSENFRPCKRCKPNGLTLPNEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTF
HHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KKMTAISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGIENPEYFATLFKKKTGFTPTE
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
YRKKNEMKERYNNEFLQK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHNEGVTLTNEHWQAIIHNDSSYDSKFFYAVKSTGVFCRPSCKSRIPNKNNVRIFHHAEQ
CCCCCCEEEHHHHEEEEECCCCCCCCEEEEEECCCEEECCCHHHCCCCCCCEEEEHHHHH
ALSENFRPCKRCKPNGLTLPNEEWVEQIKEYIEKHFDEALTLDILAEMCHGSPFHLQRTF
HHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KKMTAISPIEYIQQFRIVKAAEHLLHTNQPIKEISTAVGIENPEYFATLFKKKTGFTPTE
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHH
YRKKNEMKERYNNEFLQK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]