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The map label for this gene is dhbE [H]

Identifier: 49185215

GI number: 49185215

Start: 2204049

End: 2205665

Strand: Direct

Name: dhbE [H]

Synonym: BAS2206

Alternate gene names: 49185215

Gene position: 2204049-2205665 (Clockwise)

Preceding gene: 49185214

Following gene: 49185216

Centisome position: 42.15

GC content: 38.1

Gene sequence:

>1617_bases
ATGTTAACAGGTTATACAAAATGGCCAAAAGAATTTGCCGATCGCTATCGAGAGGAAGGGTGTTGGCTTGGGGAAACATT
TGGCGGAGTGTTAAGAGAGCGTGCTGAAAAATATGGAGATCAAATTGCGGTTGTAAGTGGTAATACGTATATAACATATA
GTGAGCTTGATAAAAAAGTAGATCGCTTAGCGTCAGGTTTACTGAATTTAGGAATAAAGCCAGAAGATCGAGTTGTAATC
CAACTACCTAATGTTATCGAGTTTTTTGAAATGTGTTTTGCACTATTTCGAATTGGAGCACTTCCTGTTTTTGCATTACC
TTCACATCGCAGTAGTGAAATTAGTTATTTTTGTGAGTTTGGTGAGGCAAGTGCGTACGTTATTTCAGATAAGGCTCTTG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGAAAGAGAAAGTACCAACTTTACAACATGTAATTGTAGTGGGAGAAAAA
GAAGAGTTTGTGAACATAAGTGATTTATATATAGATCCCGTTCTATTGCCAGAAGTTCAGCCAAGTGATGTTGCGTTTCT
CCAATTATCAGGAGGGACAACAGGACTTTCTAAATTAATTCCTAGAACGCATGATGACTATATTTATAGTTTACGTGTTA
GCGCTGAAATTTGTAATTTAAGTGCAGAGAGCGTCTATATGGCAGTTCTTCCAGTAGCACATAATTACCCAATGAGTTCT
CCTGGAACATTTGGAACCTTTTATGCGGGTGGAAAAGTTGTGCTGGCAACTGGGGGTAGTCCGGATGAGGCATTTGCACT
TATCGAAAAAGAAAAAGTTACGATTACTGCTCTCGTACCGCCATTAGCAATGATATGGCTTGACGCTGCATCTTCACGTA
ATAACGATTTATCAAGCCTAGAAGTGATTCAAGTAGGTGGTGCAAAATTTAGTGCTGAAGTTGCAAAACGGATACGTCCT
ACATTTGGATGTACATTACAGCAAGTGTTCGGTATGGCAGAAGGATTAGTGAACTATACAAGATTAGATGATCCGGAAGA
AATTATAATTCATACACAAGGTAGACCAATGTCTGCACTTGATGAAGTACGAGTTGTTGACGAATATGATAACGATGTAA
AACCTGGTGAAGTAGGTAGTTTATTAACACGGGGTCCATATACAATTCGTGGTTATTATAAAGCAGAAGAGCATAATGCA
CGATCATTTACAAATGATGGATTTTATCGCACAGGAGATCTTGTAAAAGTAAATGAACAAGGTTACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAATCGTGGTGGTGAGAAAGTCGCTGCGGAAGAAGTTGAAAATCATCTATTAGCACATGATG
CAGTACATGATGTAGCGATTGTATCTATGCCTGACGATTATTTAGGAGAGCGTACTTGTGCATTTGTAATAGCTCGCGGA
CAAGCTCCGGCAGTAAGTGAATTAAAAAGGTTCTTAAGAGAACGCGGTATAGCAGCTTATAAGATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGTGTTGGAAAGGTAAGCAAAAAAGAATTACGTAAAGTCATTGCGGAAAAACTTG
TTACGGTAAAAAGATAA

Upstream 100 bases:

>100_bases
AGAAGATGAATTAGCAGAAACATCAGCTAAGTTTCAAACAATGTTGAAAGCTTTAGGGTTAAATGATAGTTCACTTAATG
AAAAGTAGGGGGCAGAAAGA

Downstream 100 bases:

>100_bases
AAAAATATAAAACTTGGAAGGAAGTGATTAGATGGCTATCCCATCTATTTCAGTATACAAAATGCCAATTGAATCAGAAT
TACCAAAAAATAAAGTGAAT

Product: 2,3-dihydroxybenzoate-AMP ligase

Products: diphosphate; (2,3-dihydroxybenzoyl)adenylate

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEK
EEFVNISDLYIDPVLLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTNDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR

Sequences:

>Translated_538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEK
EEFVNISDLYIDPVLLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTNDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR
>Mature_538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEK
EEFVNISDLYIDPVLLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTNDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=551, Percent_Identity=25.0453720508167, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI187761345, Length=541, Percent_Identity=26.2476894639556, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI187761343, Length=541, Percent_Identity=26.2476894639556, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI28416953, Length=522, Percent_Identity=25.4789272030651, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI38505220, Length=499, Percent_Identity=23.6472945891784, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI42544132, Length=477, Percent_Identity=23.8993710691824, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI157311624, Length=512, Percent_Identity=25.1953125, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI157311622, Length=512, Percent_Identity=25.1953125, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=24.2125984251968, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI58082049, Length=512, Percent_Identity=24.609375, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI122937307, Length=498, Percent_Identity=23.4939759036145, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI8923896, Length=567, Percent_Identity=24.6913580246914, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI253970489, Length=580, Percent_Identity=24.8275862068966, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786810, Length=542, Percent_Identity=49.0774907749078, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI145693145, Length=533, Percent_Identity=31.5196998123827, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=27.6437847866419, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=24.4741873804971, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790505, Length=546, Percent_Identity=23.4432234432234, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789201, Length=356, Percent_Identity=25, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1786529, Length=522, Percent_Identity=23.3716475095785, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786801, Length=449, Percent_Identity=21.1581291759465, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=552, Percent_Identity=27.3550724637681, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71994694, Length=552, Percent_Identity=27.3550724637681, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71994690, Length=552, Percent_Identity=27.3550724637681, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=27.9026217228464, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17557194, Length=532, Percent_Identity=26.6917293233083, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=527, Percent_Identity=25.8064516129032, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=559, Percent_Identity=26.654740608229, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17559526, Length=530, Percent_Identity=25.0943396226415, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=517, Percent_Identity=25.3384912959381, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=490, Percent_Identity=21.2244897959184, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=535, Percent_Identity=23.7383177570093, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=482, Percent_Identity=21.9917012448133, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71982997, Length=565, Percent_Identity=24.9557522123894, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71983001, Length=565, Percent_Identity=24.9557522123894, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=24.5179063360882, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193209603, Length=475, Percent_Identity=21.4736842105263, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17556356, Length=355, Percent_Identity=24.5070422535211, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=385, Percent_Identity=27.5324675324675, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323182, Length=545, Percent_Identity=24.7706422018349, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319264, Length=527, Percent_Identity=22.011385199241, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=547, Percent_Identity=25.5941499085923, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18859661, Length=362, Percent_Identity=24.8618784530387, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355181, Length=531, Percent_Identity=23.1638418079096, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24667955, Length=554, Percent_Identity=25.2707581227437, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19922652, Length=535, Percent_Identity=22.2429906542056, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI62472339, Length=535, Percent_Identity=25.2336448598131, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24656500, Length=533, Percent_Identity=23.2645403377111, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355225, Length=567, Percent_Identity=22.0458553791887, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24667959, Length=382, Percent_Identity=24.869109947644, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648260, Length=534, Percent_Identity=21.7228464419476, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356947, Length=505, Percent_Identity=20.1980198019802, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356441, Length=498, Percent_Identity=22.4899598393574, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281365686, Length=475, Percent_Identity=22.9473684210526, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648253, Length=524, Percent_Identity=21.9465648854962, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648255, Length=524, Percent_Identity=21.9465648854962, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921316, Length=239, Percent_Identity=27.1966527196653, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653035, Length=305, Percent_Identity=21.3114754098361, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=20.8416833667335, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58

Molecular weight: Translated: 59754; Mature: 59754

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKV
CCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCEEEEEECCEEEEHHHHHHHH
DRLASGLLNLGIKPEDRVVIQLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEF
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEC
GEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEKEEFVNISDLYIDPVLLPEVQ
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECHHHEEEHHHEEECCEECCCCC
PSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
CCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEEEHHHHCCCHHHEEEEEEEHHCCCCCCC
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSL
CCCCEEEECCCEEEEEECCCCCHHEEEEECCCEEEEEEHHHHHHHHHHCCCCCCCCCCCE
EVIQVGGAKFSAEVAKRIRPTFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSAL
EEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
DEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNARSFTNDGFYRTGDLVKVNEQ
HHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEECCCEEEECCC
GYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCEEEEEEECC
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR
CCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKV
CCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCEEEEEECCEEEEHHHHHHHH
DRLASGLLNLGIKPEDRVVIQLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEF
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEC
GEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEKEEFVNISDLYIDPVLLPEVQ
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECHHHEEEHHHEEECCEECCCCC
PSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
CCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEEEHHHHCCCHHHEEEEEEEHHCCCCCCC
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSL
CCCCEEEECCCEEEEEECCCCCHHEEEEECCCEEEEEEHHHHHHHHHHCCCCCCCCCCCE
EVIQVGGAKFSAEVAKRIRPTFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSAL
EEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
DEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNARSFTNDGFYRTGDLVKVNEQ
HHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEECCCEEEECCC
GYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCEEEEEEECC
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLVTVKR
CCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 2,3-dihydroxybenzoate

Specific reaction: ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]