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The map label for this gene is ilvB [H]

Identifier: 49184325

GI number: 49184325

Start: 1340335

End: 1342071

Strand: Direct

Name: ilvB [H]

Synonym: BAS1308

Alternate gene names: 49184325

Gene position: 1340335-1342071 (Clockwise)

Preceding gene: 49184324

Following gene: 49184326

Centisome position: 25.63

GC content: 39.78

Gene sequence:

>1737_bases
TTGATAAAAGTTTTTAAAAGGGGGCTTATGAGAACAATGTCTTCAAAAACGGAAGAGAAACTGGCAACCGGTGCACAGCT
ATTGTTAGAAGCGCTAGAAAAGGAAGGAGTAGAAGTGATTTTTGGTTATCCTGGTGGTGCAGTTTTACCTCTTTATGATG
CGCTCTATGACTGTGAAATTCCGCATATTTTAACAAGGCATGAACAAGGAGCTATTCACGCCGCGGAAGGGTATGCCAGA
ATTACTGGCAATCCAGGGGTTGTCATTGCAACGAGTGGACCAGGTGCTACGAATGTAATTACTGGTCTAGCAGATGCGAT
GATTGATTCATTGCCACTTGTTGTATTTACAGGGCAAGTAGCAACAACGTTAATTGGAAGCGATGCTTTCCAAGAAGCGG
ATATTATGGGGCTTACGATGCCAGTGACAAAACATAACTATCAAGTGCGAAAGGCTTCAGATTTACCTCGAATTATTAAA
GAAGCATTTCATATTGCTAGAACAGGAAGACCAGGCCCAGTCGTCATTGACCTTCCGAAAGATATGGTAGTAGAGCAAGG
CGAACGATGTAGTGATGTGCAAATGGATTTACCAGGATACAACCCTAATTATGAACCGAATCTACTGCAAATAAACAAAT
TATTACAAGCAATTCAGGTTGCAAACAAGCCATTAATTTTAGCTGGAGCAGGTGTATTGCATGCGAAAGCTTCAAAAGAA
TTAACAAGTTTTGCTCGTAAATATGAAATTCCTGTTGTGCATACATTACTTGGTCTTGGTGGGTTTCCACCAGATGATGA
ACTATTTCTAGGGATGGGAGGAATGCATGGTTCTTACACAGCTAATATGGCGTTATATGAATGCAACTTACTCATTAATA
TAGGCGCTAGATTCGATGATCGTCTTACTGGAAATTTAGCTTATTTTGCGAAAGAGGCGACTGTCGCGCATATCGATATT
GATCCAGCAGAAATCGGAAAAAATGTACCGACTGAAATTCCTATTGTTGCAAGTGCTAAGCAAGCGCTCCAAGTATTGCT
TCAACCAGAAGGAAGGAAAGAAAATCATCATGAGTGGATCTCTTTATTAAAGAATAGAAAAGAAAAGTATCCATTCTCTT
ATAAACGAAATTCTGAAAGTATTAAGCCTCAATATGCAATTGATATGTTATATGAAATTACGAAGGGAGAAGCGATTGTA
ACAACAGATGTGGGGCAACATCAAATGTGGGCAGCCCAATATTATCCGCTCAAAACTCCAGATAAATGGGTAACTTCAGG
AGGACTAGGAACGATGGGATTTGGATTTCCGGCAGCAATTGGTGCACAAATTGCAAAGCCTGATGAACTAGTTATTGCAA
TTGTCGGTGATGCTGGATTTCAAATGACTCTGCAAGAATTAAGTGTGTTAAAAGAACACTCTTTACCTGTTAAAGTATTT
ATTTTAAATAATGAAGCTTTAGGAATGGTAAGACAATGGCAAGATGAATTTTATAACCAAAGATATTCGCACTCTTTACT
ATCGTGTCAACCAGATTTCGTTGCTCTTGCGAATGCGTATGGCATAAAAGGGGTTCGCATAGATGATCCACTTCTTGCGA
AGAAACAATTGCAGCATGCAATTGAATTACAAGAACCAGTCGTAATTGATTGCCGTGTACTTCAATCAGAAAAGGTAATG
CCGATGGTTGCGCCAGGGAAAGGTGTTCACCAAATGGAGGGAGTGGAAAAAAGGTGA

Upstream 100 bases:

>100_bases
TGGGTCACTGAGGCAAGAGTATTCTTGCAAAGAAGGGTGGTACCGCGAAATCTTTCGTCCCTTCAGCACATAGCTGGAGT
GTGGACGTAGGATTTTTTTA

Downstream 100 bases:

>100_bases
AGAGAATTGTTACAGCAACAGTTCGAAATCAAAGTGGTGTGTTAAACCGAATTACAGGTGTTATGACACGAAGACATTTT
AATATTGAAAGTATTTCAGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYAR
ITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIK
EAFHIARTGRPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKE
LTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECNLLINIGARFDDRLTGNLAYFAKEATVAHIDI
DPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIV
TTDVGQHQMWAAQYYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVF
ILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIELQEPVVIDCRVLQSEKVM
PMVAPGKGVHQMEGVEKR

Sequences:

>Translated_578_residues
MIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYAR
ITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIK
EAFHIARTGRPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKE
LTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECNLLINIGARFDDRLTGNLAYFAKEATVAHIDI
DPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIV
TTDVGQHQMWAAQYYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVF
ILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIELQEPVVIDCRVLQSEKVM
PMVAPGKGVHQMEGVEKR
>Mature_578_residues
MIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEIPHILTRHEQGAIHAAEGYAR
ITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQVATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIK
EAFHIARTGRPGPVVIDLPKDMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKE
LTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECNLLINIGARFDDRLTGNLAYFAKEATVAHIDI
DPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWISLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIV
TTDVGQHQMWAAQYYPLKTPDKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVF
ILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHAIELQEPVVIDCRVLQSEKVM
PMVAPGKGVHQMEGVEKR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.0282685512367, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI21361361, Length=513, Percent_Identity=23.9766081871345, Blast_Score=139, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=45.9363957597173, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=40.8127208480565, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=517, Percent_Identity=35.5899419729207, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=27.3897058823529, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788716, Length=484, Percent_Identity=24.1735537190083, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=485, Percent_Identity=28.8659793814433, Blast_Score=165, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=485, Percent_Identity=28.8659793814433, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=558, Percent_Identity=24.0143369175627, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=44.5026178010471, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=24.0246406570842, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=24.2857142857143, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922626, Length=486, Percent_Identity=26.3374485596708, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63400; Mature: 63400

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCC
PHILTRHEQGAIHAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQV
CHHHHCCCCCCEEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHH
ATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGPVVIDLPK
HHHHHCCCHHCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCH
DMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKE
HHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHH
LTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECNLLINIGARFDD
HHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEEEEEEEEEECCCCCC
RLTGNLAYFAKEATVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWI
CCCCCEEEEEECCEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCCCHHHHHH
SLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQYYPLKTP
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCC
DKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVF
CHHCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEE
ILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHA
EECCCHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHH
IELQEPVVIDCRVLQSEKVMPMVAPGKGVHQMEGVEKR
HHCCCCEEEEEEEECCCCCCEEECCCCCCHHCCCCCCC
>Mature Secondary Structure
MIKVFKRGLMRTMSSKTEEKLATGAQLLLEALEKEGVEVIFGYPGGAVLPLYDALYDCEI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCC
PHILTRHEQGAIHAAEGYARITGNPGVVIATSGPGATNVITGLADAMIDSLPLVVFTGQV
CHHHHCCCCCCEEECCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHH
ATTLIGSDAFQEADIMGLTMPVTKHNYQVRKASDLPRIIKEAFHIARTGRPGPVVIDLPK
HHHHHCCCHHCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCH
DMVVEQGERCSDVQMDLPGYNPNYEPNLLQINKLLQAIQVANKPLILAGAGVLHAKASKE
HHHHHCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHH
LTSFARKYEIPVVHTLLGLGGFPPDDELFLGMGGMHGSYTANMALYECNLLINIGARFDD
HHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEEEEEEEEEECCCCCC
RLTGNLAYFAKEATVAHIDIDPAEIGKNVPTEIPIVASAKQALQVLLQPEGRKENHHEWI
CCCCCEEEEEECCEEEEEECCHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCCCHHHHHH
SLLKNRKEKYPFSYKRNSESIKPQYAIDMLYEITKGEAIVTTDVGQHQMWAAQYYPLKTP
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCC
DKWVTSGGLGTMGFGFPAAIGAQIAKPDELVIAIVGDAGFQMTLQELSVLKEHSLPVKVF
CHHCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEE
ILNNEALGMVRQWQDEFYNQRYSHSLLSCQPDFVALANAYGIKGVRIDDPLLAKKQLQHA
EECCCHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHCCCCCEEECCHHHHHHHHHHH
IELQEPVVIDCRVLQSEKVMPMVAPGKGVHQMEGVEKR
HHCCCCEEEEEEEECCCCCCEEECCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]