Click here to switch to the map view.

The map label for this gene is ppnK

Identifier: 49184140

GI number: 49184140

Start: 1174207

End: 1175004

Strand: Direct

Name: ppnK

Synonym: BAS1120

Alternate gene names: 49184140

Gene position: 1174207-1175004 (Clockwise)

Preceding gene: 49184139

Following gene: 49184141

Centisome position: 22.46

GC content: 39.97

Gene sequence:

>798_bases
ATGAAATTTACAATTATGTCAAAGGGAGATCAATCATCAGATACACTGGCAAGCACGATGAAAGAATACTTGCTAGATTT
TGGATTTATAATGGATGAACAGGAACCCGATATTGTAATTTCTGTTGGGGGAGATGGAACGCTTTTATATGCGTTTCATC
GTTATTATAATCGATTGGATGAAACAGCATTTGTTGGTGTACATACAGGGCATTTAGGTTTCTACGCAGATTGGTTACCG
ACAGAAGTAGAAAAATTAGTAATTGCAATTGCTAAAACACCATTCCAAGTGGTGGAATATCCACTTTTAGAAGTGATTAT
TCGCTATATGAATGGGAGTAAAGAGTCACAGTATTTAGCGATGAATGAAGCGACTGTGAAAAGTGCAGAAGGTACATTAG
TAACAGAAGTAGAAATACGCGGAGAGTACTTTGAAACGTTCCGCGGGGATGGCCTTTGTATTTCTACTCCTTCGGGAAGT
ACTGCGTATAATAAGGCGCTTGGCGGAGCAATTATTCACCCTTCTATTGAAGCGATTCAAATTGCAGAAATGGCATCGAT
TAATAACCGTGTGTTTCGTACGGTAGGTTCGCCACTCGTATTGCCGAAACATCATACATGTGTGTTGAAGCCGACTGCAG
GAATGAACTTACAAATTACGGTGGACCATTTAACGATGGTTCATCAAGATGTGAAATCAATTCAGTATCGCGTGGCAAAC
GAGAAAGTACGATTTGTTCGTTTCCGTCCATTTCCGTTCTGGAAACGAGTTCGTGACTCGTTTGTTGCAGATAAATAG

Upstream 100 bases:

>100_bases
AATGTCTTCTATTCGTCTTGAAATTCAAGAAGCACAGAAAGCTTTTTCAAGAAAGCAAGAAACGAAAGATTCTGAATCAT
AAACTAAAGGGTGTTGGGCG

Downstream 100 bases:

>100_bases
AAAGGGTTTATATATTTGAACAGATTTACATTGAAATGGGATATAGAATCGGCTGAAGAAGGAACTTTAGTTAGAGAATT
TTTAAAAACAAAAGGGATCT

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKFTIMSKGDQSSDTLASTMKEYLLDFGFIMDEQEPDIVISVGGDGTLLYAFHRYYNRLDETAFVGVHTGHLGFYADWLP
TEVEKLVIAIAKTPFQVVEYPLLEVIIRYMNGSKESQYLAMNEATVKSAEGTLVTEVEIRGEYFETFRGDGLCISTPSGS
TAYNKALGGAIIHPSIEAIQIAEMASINNRVFRTVGSPLVLPKHHTCVLKPTAGMNLQITVDHLTMVHQDVKSIQYRVAN
EKVRFVRFRPFPFWKRVRDSFVADK

Sequences:

>Translated_265_residues
MKFTIMSKGDQSSDTLASTMKEYLLDFGFIMDEQEPDIVISVGGDGTLLYAFHRYYNRLDETAFVGVHTGHLGFYADWLP
TEVEKLVIAIAKTPFQVVEYPLLEVIIRYMNGSKESQYLAMNEATVKSAEGTLVTEVEIRGEYFETFRGDGLCISTPSGS
TAYNKALGGAIIHPSIEAIQIAEMASINNRVFRTVGSPLVLPKHHTCVLKPTAGMNLQITVDHLTMVHQDVKSIQYRVAN
EKVRFVRFRPFPFWKRVRDSFVADK
>Mature_265_residues
MKFTIMSKGDQSSDTLASTMKEYLLDFGFIMDEQEPDIVISVGGDGTLLYAFHRYYNRLDETAFVGVHTGHLGFYADWLP
TEVEKLVIAIAKTPFQVVEYPLLEVIIRYMNGSKESQYLAMNEATVKSAEGTLVTEVEIRGEYFETFRGDGLCISTPSGS
TAYNKALGGAIIHPSIEAIQIAEMASINNRVFRTVGSPLVLPKHHTCVLKPTAGMNLQITVDHLTMVHQDVKSIQYRVAN
EKVRFVRFRPFPFWKRVRDSFVADK

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=153, Percent_Identity=32.6797385620915, Blast_Score=71, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325068, Length=238, Percent_Identity=22.6890756302521, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK1_BACAN (Q81TQ3)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_843688.1
- RefSeq:   YP_017824.1
- RefSeq:   YP_027392.1
- ProteinModelPortal:   Q81TQ3
- SMR:   Q81TQ3
- EnsemblBacteria:   EBBACT00000008461
- EnsemblBacteria:   EBBACT00000018651
- EnsemblBacteria:   EBBACT00000022243
- GeneID:   1088735
- GeneID:   2815246
- GeneID:   2848724
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_1213
- KEGG:   bar:GBAA_1213
- KEGG:   bat:BAS1120
- TIGR:   BA_1213
- TIGR:   GBAA_1213
- GeneTree:   EBGT00050000002705
- HOGENOM:   HBG314020
- OMA:   TISFANC
- ProtClustDB:   PRK04885
- BioCyc:   BANT260799:BAS1120-MONOMER
- BioCyc:   BANT261594:GBAA1213-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 29829; Mature: 29829

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIMSKGDQSSDTLASTMKEYLLDFGFIMDEQEPDIVISVGGDGTLLYAFHRYYNRLD
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHCC
ETAFVGVHTGHLGFYADWLPTEVEKLVIAIAKTPFQVVEYPLLEVIIRYMNGSKESQYLA
CCEEEEEEECCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
MNEATVKSAEGTLVTEVEIRGEYFETFRGDGLCISTPSGSTAYNKALGGAIIHPSIEAIQ
ECCHHHCCCCCCEEEEEEECCHHHHHHCCCCEEEECCCCCCHHHHHCCCEEECCCCHHHH
IAEMASINNRVFRTVGSPLVLPKHHTCVLKPTAGMNLQITVDHLTMVHQDVKSIQYRVAN
HHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
EKVRFVRFRPFPFWKRVRDSFVADK
CEEEEEEECCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKFTIMSKGDQSSDTLASTMKEYLLDFGFIMDEQEPDIVISVGGDGTLLYAFHRYYNRLD
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEHHHHHHHHHCC
ETAFVGVHTGHLGFYADWLPTEVEKLVIAIAKTPFQVVEYPLLEVIIRYMNGSKESQYLA
CCEEEEEEECCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
MNEATVKSAEGTLVTEVEIRGEYFETFRGDGLCISTPSGSTAYNKALGGAIIHPSIEAIQ
ECCHHHCCCCCCEEEEEEECCHHHHHHCCCCEEEECCCCCCHHHHHCCCEEECCCCHHHH
IAEMASINNRVFRTVGSPLVLPKHHTCVLKPTAGMNLQITVDHLTMVHQDVKSIQYRVAN
HHHHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
EKVRFVRFRPFPFWKRVRDSFVADK
CEEEEEEECCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629