Click here to switch to the map view.

The map label for this gene is asnO [H]

Identifier: 49184094

GI number: 49184094

Start: 1126065

End: 1127843

Strand: Direct

Name: asnO [H]

Synonym: BAS1074

Alternate gene names: 49184094

Gene position: 1126065-1127843 (Clockwise)

Preceding gene: 49184092

Following gene: 49184095

Centisome position: 21.54

GC content: 37.77

Gene sequence:

>1779_bases
ATGGCTGAGACGTTAGCGAAGCGTGGTCCAGATGATAATAAAGTTTGGATTAAAGGCAATGTCGCATTTGGGCATAAACG
GTTAATCGTTGTTGATCCTGAAGGTGGTAAACAGCCGATGACTTGTTTAAAGGATGAAGCAAATTATGCAATTTGCTATA
ACGGCGAACTGTATAACACGGAAGACATTCGAAAGGAACTATTAAGAAGAGGATATACGTTCAAAGGTCACTCTGATACG
GAAGTATTGTTAGCTTCTTATATCGAATGGAAAGAAGAATGTGTCGATCATTTAAACGGTATATATGCGTTTGCTGTATG
GGATGAACAGAAAGAACAAGTGTTTATTGCGCGCGATCGATTAGGGGTAAAACCACTATTTTATAAATATGACAGTGGGC
GATTACTATTTGGCTCAGAGTTAAAAGCGATATTGGCGCATCCAGATGTGAAGGCGGAAGTGACGTTAGAAGGATTATCT
GAAATATTTGGCCTTGGACCATCTAGAACGCCTGGTCACGGTATTTATACTGGCATAAAAGAATTACGTCCAGGTCATGC
GATGACATTTTCGAAGAACGGTTTATGTATATGGAGATATTGGAATGTAGAAAGTAAAAAGCATGAAGATTCTTTTGAAG
AAACAGTAGAAAAAACACGCTTTTTATTACAAGATGCGATTACAAGGCAGCTCGTTTCTGATGTACCATTATGCACTTTT
TTATCAGGCGGTGTAGATTCAAGCGCTATAACAGCAATTGCTGCGAAAGAATACGAAAAATCGGGAAAAGGTCAATTACA
CACGTATTCGGTTGATTACGAAGATAATGACAAATACTTTAAAGCAAATGCGTTTCAGCCAAATTCAGATGCACCATTTA
TTCATTTGATGACTGAGACGTTTCAAACGATCCATCATCGTTGCGTCATTTCAAATGAACAATTAGCGCAGTATTTAACA
GAAGCAGTACTCGTTCGTGATTTGCCTGGTATGGCAGATATTGATTCGTCATTATTATGGTTTTGTCGTGAAATTAAACA
AGATTTTGTCGTTGGTTTATCTGGGGAATGTGCAGATGAAATATTTGGTGGTTATCCATGGTTTTATAGAGAAGATGATT
TACAATCGAGTGCATTTCCGTGGATGCGTTCTACAGAAGCGCGTGAACAATTGCTAAAGAAAGAATGGAGAAATAAATTA
AATTTACAACAATATGTACAGCAGCGCTATGAAGAATCCATTCAAGAAGTTCCTATTTTAGATGGGGAAAGTCCGCTAGA
AGCAAAAAGACGCCAATTATTTTATTTAAATATGGTATGGTTTATGACAACATTATTAGACAGAAAAGACCGCATGAGTA
TGGGGGCAAGCTTAGAAGTGCGCGTTCCATTTGCAGATCACCGACTTGTCGAATATGCGTGGAATATTCCTTGGGAAATG
AAAATGTATAAAAACCGCGAAAAAGGTCTATTGCGTAAAGCGTTAGAAGGGTTACTTCCAAATGACATCTTATATAGAAA
GAAGAGCCCGTATCCAAAAACACATAATCCGCACTATACGAAAGCAGTAACAGTATGGATACAAGATTTATTAACGGATA
AAGGCTCAATTTTACACGAGCTATTTGATAAAGAACAGCTGAGTGGATTAATTGAGTCAGGCGGCAGTGCATTCCAAACG
CCATGGTATGGTCAATTAATGACTGGGCCACAGCTTTTAGCTCATTTAGCACAAATTCATGTTTGGTTTAAAGAATATGG
AGTAAATATTAAAGAATAG

Upstream 100 bases:

>100_bases
AGAAAAGAAATAAGATGGAAGGATGATTTATATGTGCGGGATTACAGGATGGGTGGATTATAAACGCTCATTAGAAGGAG
AACGGGACGTCGTTACGAAG

Downstream 100 bases:

>100_bases
GAGAAAAGCGACTTACTTTATAAGTAAGTCGCTTTTTATTAAATAAATTTTCATATAAAATTCAAAATCATATTAGAAAA
AATACTTATTTAGAATAATT

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDT
EVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLS
EIFGLGPSRTPGHGIYTGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
LSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLT
EAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKL
NLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
KMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHELFDKEQLSGLIESGGSAFQT
PWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Sequences:

>Translated_592_residues
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDT
EVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLS
EIFGLGPSRTPGHGIYTGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
LSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLT
EAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKL
NLQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
KMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHELFDKEQLSGLIESGGSAFQT
PWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
>Mature_591_residues
AETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNTEDIRKELLRRGYTFKGHSDTE
VLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDRLGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSE
IFGLGPSRTPGHGIYTGIKELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTFL
SGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTETFQTIHHRCVISNEQLAQYLTE
AVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADEIFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLN
LQQYVQQRYEESIQEVPILDGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEMK
MYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHELFDKEQLSGLIESGGSAFQTP
WYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=543, Percent_Identity=25.7826887661142, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI168229252, Length=543, Percent_Identity=25.7826887661142, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI168229250, Length=543, Percent_Identity=25.7826887661142, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI296010848, Length=540, Percent_Identity=25.7407407407407, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI296010852, Length=470, Percent_Identity=24.0425531914894, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI296010850, Length=470, Percent_Identity=24.0425531914894, Blast_Score=105, Evalue=2e-22,
Organism=Escherichia coli, GI1786889, Length=534, Percent_Identity=25.8426966292135, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17560178, Length=583, Percent_Identity=25.7289879931389, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325403, Length=547, Percent_Identity=25.0457038391225, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321563, Length=546, Percent_Identity=25.2747252747253, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45553209, Length=524, Percent_Identity=25.763358778626, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 68315; Mature: 68183

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNT
CCCCHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCEECH
EDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDR
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCC
LGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYTGIK
CCCCEEEEEECCCCEEECCHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHH
ELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
HCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTET
HCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEHHHHH
FQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADE
HHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHH
IFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPIL
HHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCHHHHHHHCCCEEE
KMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHE
HHHHCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
LFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AETLAKRGPDDNKVWIKGNVAFGHKRLIVVDPEGGKQPMTCLKDEANYAICYNGELYNT
CCCHHHCCCCCCEEEEEEEEEECCEEEEEECCCCCCCCHHHHHCCCCEEEEECCCEECH
EDIRKELLRRGYTFKGHSDTEVLLASYIEWKEECVDHLNGIYAFAVWDEQKEQVFIARDR
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCC
LGVKPLFYKYDSGRLLFGSELKAILAHPDVKAEVTLEGLSEIFGLGPSRTPGHGIYTGIK
CCCCEEEEEECCCCEEECCHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHH
ELRPGHAMTFSKNGLCIWRYWNVESKKHEDSFEETVEKTRFLLQDAITRQLVSDVPLCTF
HCCCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LSGGVDSSAITAIAAKEYEKSGKGQLHTYSVDYEDNDKYFKANAFQPNSDAPFIHLMTET
HCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCEEEHHHHH
FQTIHHRCVISNEQLAQYLTEAVLVRDLPGMADIDSSLLWFCREIKQDFVVGLSGECADE
HHHHHHHHEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHH
IFGGYPWFYREDDLQSSAFPWMRSTEAREQLLKKEWRNKLNLQQYVQQRYEESIQEVPIL
HHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
DGESPLEAKRRQLFYLNMVWFMTTLLDRKDRMSMGASLEVRVPFADHRLVEYAWNIPWEM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCCCHHHHHHHCCCEEE
KMYKNREKGLLRKALEGLLPNDILYRKKSPYPKTHNPHYTKAVTVWIQDLLTDKGSILHE
HHHHCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHH
LFDKEQLSGLIESGGSAFQTPWYGQLMTGPQLLAHLAQIHVWFKEYGVNIKE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]