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The map label for this gene is pbpF [H]

Identifier: 49184019

GI number: 49184019

Start: 1048770

End: 1050887

Strand: Direct

Name: pbpF [H]

Synonym: BAS0998

Alternate gene names: 49184019

Gene position: 1048770-1050887 (Clockwise)

Preceding gene: 49184017

Following gene: 49184020

Centisome position: 20.06

GC content: 39.75

Gene sequence:

>2118_bases
ATGAAGAAAGTAAAGTGGACTTTATTAGGTGGGTTAGCAACGCTTGTATTAGCGATTGTACTTTATAAACTTATCGTGTT
AGCTGGTGGCTATATGATGGATGAAAAACAGCTCGTTTTCCACTCTTCATCACGTATCGTTGACCAGAAAGGGAAAGAAA
TTACGAAATTATACGTAGAAAATAGAGACCTCGTACCAATCGAACAAATTCCAAAATATGTGCAGCAGGCGTTTGTTGCG
GTAGAGGATTCTCGTTTTTATGAGCATCAAGGAATCGATTATCCGTCTATATTCCGCGCTCTGTATAAAGACACGTTAGC
TGGAGAGAAGGTCGAAGGCGGCAGTACGATTACGCAGCAACTTGCTAAAAACGTCTTTTTAACTCGTGAAAAAACATTTA
CGCGCAAGTTGAAGGAAGTCGCAATTTCCCTTCAATTAGAGCAAAAATATACGAAGCAGCAAATTCTTGAGATGTATATG
AATCATATTTATTTTGGTCATGGAGCATACGGTATTCAAGCGGCAGCAAAGTTGTATTTTAATAAAAATGTAGAAGATTT
AACAGTAGAAGAAGGGGCAATGCTTGCAGGTCTTCCGAAGTCGCCAAACGGATATTCACCTTATTTCTCTCCAGAGAAAA
GTAAGGAGCGTCGCGATCTCGTATTGTCACTTATGCATAAACAAGGCTATTTAACTGCCGAAGAAAGCGTGCGTTATCAA
GGAAAAACAATTGCTCTTTATAAAAACTTAGATGAGAATGAGCTCGCATATATGCCGTATATTGATATGGTCATAGATGA
AGCGGCTCGTTTATACGGGTTGTCTCATCAAGAAGTACTCCGCGGAGGATATACGTTTGTCGTACCGATGGATGAAAAAA
TTCAAAAGGTAGCGTATAACCAGTTTCAAGATGCAAGGAATTTCCCTGGGAAAGAAGAGGGGGCGCAAGGTGCATTTTTA
TTAATGGATAATCGTACGGGCGGGATTAAAGCGGCGATTGGCGGAAGAAAGTACGTCCCGAGAGGATTTAATCGTGTTTT
TGCGAAAAGACAACCTGGTTCTGTACTAAAACCACTGATCGTCTATGCACCAGCACTCGAAACGAAAAAGTATAATCCGT
ATTCTTTATTGACGAATGAAAGACATTCTTTTGAAGGGTATGAACCTCGAAACTATAACCGTGAATATTCAAAAGAAATA
ACGATGTACGATGCGATTTTAGAGTCAGCAAATGTACCGGCAGTTTCTTTATTAAACGAGTTGGGGGTAGAAGAAGGAAA
GCAATATTTAGAGAAAGGAAATGTTCACATTGCCGATGCTGGTTTAAGCACAGCGCTTGGTGGATTGAAAAATGGTGTTT
CAACATTTGATCTTGTGAAAATGTATCGTGCGTTTTTAGCAAATGGTGATATTGTTGAGCCGCACGTTATTGATAAAGTG
TTAAATAGACACGGCGCAGTCATTGGAGAATCTCCAAAAGTAGAAACGAAAATTTTCTCGAAACAAACGGCATGGTATAT
GACGAAAATGTTAGAAGGAGTTGTAAAGGAAGGGACAGCGAAAGCCGGTGTATATAGCGGAGCGTTAGCTGGGAAAACAG
GTACAACTTCCTTACCAAATGACGATAATGGGGCGAGAGATATGTGGTTCGTTGGCTATACGCCAAATTTAGTAGGCGCT
GTTTGGATTGGTTATGACCGCATAGATAAAGAGCATAAGTTACAAGGAGAAAGTGCGTCAGCAACAAAATTGTTTAAGAA
AATTTTAACGAAAGCAAATGTAGAACAGAAAGAGCAGTTTAGGAAACCAGAAGGTGTGGAAACAATCGGTGCTCCAATTC
GGCTGCGCAAGATTGAAGATGTGAAAATGAAATTAGCGTTTAGCCCTTTCGGTTTATTTAAAGCGAAATTAAGCTGGACA
CCACTTCCAGATGAAAGAATTATGTATCGTATTTATAGAGTGGAAAACGGAATTCATACGCACGTAGGTACTGTAAACGG
TGCTGGGGAATATGAGGAAAAGTTCGTTAACATCTTTTCAAAACCGAGTTTTTACGTTGTGCCATATAACACACAAACGA
ATCGCGAAGGAGAAAAGTCAAAAGTAGCTAAACCATAG

Upstream 100 bases:

>100_bases
TATTATATCAACTCTCTTAAAAAACTTCGAAGATTATATACATAAAGATACTTCTTTTTCAAATAATAAATGTACCATCA
TTTGGAAAGAAGGCCGATGT

Downstream 100 bases:

>100_bases
TTTTTCTCTGTGTTATAATAAGAATGTTGCGAAAATAGTAAGCGTTTTTACGAATGTTTGTGTCAATTTAATGAACAACG
TACATAATATTATGCATTTT

Product: penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRDLVPIEQIPKYVQQAFVA
VEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEI
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIVEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGA
VWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWT
PLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP

Sequences:

>Translated_705_residues
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRDLVPIEQIPKYVQQAFVA
VEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEI
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIVEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGA
VWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWT
PLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
>Mature_705_residues
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRDLVPIEQIPKYVQQAFVA
VEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEI
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIVEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGA
VWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWT
PLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=304, Percent_Identity=38.8157894736842, Blast_Score=195, Evalue=8e-51,
Organism=Escherichia coli, GI1786343, Length=566, Percent_Identity=28.7985865724382, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1788867, Length=538, Percent_Identity=27.1375464684015, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789601, Length=180, Percent_Identity=36.6666666666667, Blast_Score=116, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 79430; Mature: 79430

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVE
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEECCCHHHHHCCCCEEHHEEC
NRDLVPIEQIPKYVQQAFVAVEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQ
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
LAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAAKLYF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
NKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
CCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCC
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYN
CCEEEEEECCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECHHHHHHHHHH
QFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLI
HHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHH
VYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNE
HCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIVEPHVIDKV
HCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPN
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DDNGARDMWFVGYTPNLVGAVWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQF
CCCCCCEEEEEECCCCCCEEEEECHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHC
RKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHT
CCCCCCHHCCCCEEEEEECCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEE
HVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
ECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVE
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEECCCHHHHHCCCCEEHHEEC
NRDLVPIEQIPKYVQQAFVAVEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQ
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
LAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAAKLYF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
NKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
CCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCC
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYN
CCEEEEEECCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECHHHHHHHHHH
QFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLI
HHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHH
VYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNE
HCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIVEPHVIDKV
HCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPN
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DDNGARDMWFVGYTPNLVGAVWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQF
CCCCCCEEEEEECCCCCCEEEEECHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHC
RKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHT
CCCCCCHHCCCCEEEEEECCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEE
HVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
ECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]