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The map label for this gene is prsA

Identifier: 49183995

GI number: 49183995

Start: 1030131

End: 1030994

Strand: Reverse

Name: prsA

Synonym: BAS0974

Alternate gene names: 49183995

Gene position: 1030994-1030131 (Counterclockwise)

Preceding gene: 49183997

Following gene: 49183994

Centisome position: 19.72

GC content: 34.72

Gene sequence:

>864_bases
ATGAAGAAAGCTATGCTTGCCTTAGCCGCAACAAGTGTAATCGCATTATCAGCATGTGGAACATCATCATCAGACAAAAT
TGTTACATCTAAAGCTGGTGACATTACAAAAGACGAATTCTACGAGCAAATGAAGACACAAGCTGGTAAACAAGTGTTAA
ACAACATGGTTATGGAAAAAGTACTTATTAAAAACTACAAAGTTGAAGACAAAGAAGTAGACAAAAAGTACGATGAAATG
AAAAAACAATACGGTGATCAATTCGATACACTATTAAAACAACAAGGTATTAAAGAAGAAACGTTAAAAACTGGTGTGCG
TGCTCAATTAGCTCAAGAAAAAGCTATCGAGAAAACAATCACTGATAAAGAATTGAAAGACAACTACAAGCCTGAAATTA
AAGCAAGCCACATTCTTGTAAAAGATGAAGCGACTGCGAAAAAGGTGAAAGAAGAACTTGGACAAGGCAAATCTTTCGAA
GAGTTAGCGAAACAATACTCTGAAGATACTGGTTCAAAAGAAAAAGGTGGCGACTTAGGATTCTTCGGAGCTGGTAAAAT
GGTTAAAGAATTCGAAGATGCTGCTTATAAACTGAAAAAAGATGAAGTAAGCGAGCCTGTGAAATCACAATTCGGTTACC
ACATCATTAAAGTTACAGACATTAAAGAACCAGAAAAATCATTCGAGCAATCTAAAGCTGACATCAAAAAAGAATTAGTT
GCGAAGAAATCACAAGACGGCGAATTCATGAACGACCTTATGATGAAAGAAATCAAAAAAGCTGACGTAAAAGTTGACGA
TAAAGATTTGAAAGATCTTTTCGAAGAGAAAAAAGCTGACGCTAAAAAAGAAGAAAAGAAATAA

Upstream 100 bases:

>100_bases
AAAAGCAAAAGTATCAGTGAGACTTAATCGGGCTCTAATGAACTAAAGTTCATTGGTATAGGATAAATTAAAAATACATA
ATAGTAGGAGTGTTTATCGA

Downstream 100 bases:

>100_bases
TGAAATAAAAAGCTCGGGCTTAGGCCCGAGCTTTTTTTGTATACCCTCTTCTCTCGGTAGTTATGTCCGATTCGGTAAAT
GAAATTATATAGTTGGTCCT

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEKVLIKNYKVEDKEVDKKYDEM
KKQYGDQFDTLLKQQGIKEETLKTGVRAQLAQEKAIEKTITDKELKDNYKPEIKASHILVKDEATAKKVKEELGQGKSFE
ELAKQYSEDTGSKEKGGDLGFFGAGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTDIKEPEKSFEQSKADIKKELV
AKKSQDGEFMNDLMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK

Sequences:

>Translated_287_residues
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEKVLIKNYKVEDKEVDKKYDEM
KKQYGDQFDTLLKQQGIKEETLKTGVRAQLAQEKAIEKTITDKELKDNYKPEIKASHILVKDEATAKKVKEELGQGKSFE
ELAKQYSEDTGSKEKGGDLGFFGAGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTDIKEPEKSFEQSKADIKKELV
AKKSQDGEFMNDLMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK
>Mature_287_residues
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEKVLIKNYKVEDKEVDKKYDEM
KKQYGDQFDTLLKQQGIKEETLKTGVRAQLAQEKAIEKTITDKELKDNYKPEIKASHILVKDEATAKKVKEELGQGKSFE
ELAKQYSEDTGSKEKGGDLGFFGAGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTDIKEPEKSFEQSKADIKKELV
AKKSQDGEFMNDLMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK

Specific function: Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Important for the secretion of the protective antigen. The three psrA proteins in this organism show different but overlapping

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain

Homologues:

Organism=Homo sapiens, GI38679892, Length=92, Percent_Identity=41.304347826087, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1786238, Length=106, Percent_Identity=34.9056603773585, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790211, Length=84, Percent_Identity=44.047619047619, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17537235, Length=92, Percent_Identity=41.304347826087, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322478, Length=60, Percent_Identity=56.6666666666667, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356303, Length=92, Percent_Identity=41.304347826087, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PRSA1_BACAN (Q81U45)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_843541.1
- RefSeq:   YP_017668.1
- RefSeq:   YP_027247.1
- ProteinModelPortal:   Q81U45
- SMR:   Q81U45
- EnsemblBacteria:   EBBACT00000009017
- EnsemblBacteria:   EBBACT00000013942
- EnsemblBacteria:   EBBACT00000024035
- GeneID:   1088973
- GeneID:   2815617
- GeneID:   2850920
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_1041
- KEGG:   bar:GBAA_1041
- KEGG:   bat:BAS0974
- TIGR:   BA_1041
- TIGR:   GBAA_1041
- GeneTree:   EBGT00050000000187
- HOGENOM:   HBG712792
- OMA:   MLVEYAV
- ProtClustDB:   PRK03095
- BioCyc:   BANT260799:BAS0974-MONOMER
- BioCyc:   BANT261594:GBAA1041-MONOMER
- BRENDA:   5.2.1.8
- HAMAP:   MF_01145
- InterPro:   IPR023059
- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880

Pfam domain/function: PF00639 Rotamase; SSF109998 Trigger_fac_C_bac

EC number: =5.2.1.8

Molecular weight: Translated: 32456; Mature: 32456

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS01096 PPIC_PPIASE_1; PS50198 PPIC_PPIASE_2; PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEK
CCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLIKNYKVEDKEVDKKYDEMKKQYGDQFDTLLKQQGIKEETLKTGVRAQLAQEKAIEKTI
HHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TDKELKDNYKPEIKASHILVKDEATAKKVKEELGQGKSFEELAKQYSEDTGSKEKGGDLG
HHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
FFGAGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTDIKEPEKSFEQSKADIKKELV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
AKKSQDGEFMNDLMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK
HHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEK
CCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLIKNYKVEDKEVDKKYDEMKKQYGDQFDTLLKQQGIKEETLKTGVRAQLAQEKAIEKTI
HHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
TDKELKDNYKPEIKASHILVKDEATAKKVKEELGQGKSFEELAKQYSEDTGSKEKGGDLG
HHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
FFGAGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTDIKEPEKSFEQSKADIKKELV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
AKKSQDGEFMNDLMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK
HHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12721629; 12606539