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The map label for this gene is mhpC [C]

Identifier: 49183975

GI number: 49183975

Start: 1010691

End: 1011566

Strand: Direct

Name: mhpC [C]

Synonym: BAS0953

Alternate gene names: 49183975

Gene position: 1010691-1011566 (Clockwise)

Preceding gene: 49183974

Following gene: 49183976

Centisome position: 19.33

GC content: 40.75

Gene sequence:

>876_bases
ATGTTGAAACGGTTTAAGTTTTATATGATTAGCGGTATTGTGCTCGTTTTACTTATCGTATGTAACTTCGTTGATAAGCC
AATCGCTAAGGTTGGAGAAGTGAAAGATACTGTTATGATGAAGCTGAATACGAAAGAGAGTATGGCACAAATCGACGGGC
AGACGATTTATTTTAAGAAAATTGGTGAGGGGAAGCCTCCTTTACTTATGCTGCACGGGTTTGGTGGTTCTTCTGATGGA
TTTAGTGATATTTATCCGGAACTTGCGCGAGATCACACGATTATTGCAGTGGATATTTTAGGGTTTGGGCGTTCTTCAAA
GCCAATTGATTTTGAGTATTCGTTTCCTGCGCAAGTGAATTTATATTATAAGTTAATGAAGAAGCTTGGATACGATCAGT
TTGCGGTGCTCGGTCATTCGATGGGCGGAGAGATGTCCCTCAATTTAGCGTATTTATATCCGGACGCAGTGACGCATCTC
ATTTTAGCAGACTCTACAGGAATTGAATCTTTCCAGCAAAAAGAAAGTTATGAAGTGCCGCCACTATCGACAGACTTACA
AACGGTAACAGAGATTACGGATTACAATAAAAATGAAGTGAAAAATAGTCGCGATGATAAAGGGCATTATGATCAGCTGA
CGAAAATGAGAGAGCGCCGCATTGCGATGGAAGCCGATAAAATTAAGGTGCCGACTTTAATCATATGGGGACGACACGAT
AAGAGCGTTTCTTGGAAAAATGGTGAACTGTATCATGGGCTATTTGCGAATAGCACGTTCCATATTATTGAAAAAGGATA
CCATGCACCGTTTCGCCAGGAACCAATCGAGTTTATGGAATATGTACAAGCGTTCTTCGCGAAGAATCCACAATGA

Upstream 100 bases:

>100_bases
TTTAATATAATTGTAACCGTTGTGGTTACAATTAAAATTAATTATAAAACAACCAAAAAATGTAAAAACTTTAAATAACA
ATTTGAGAAAGGAGACGGGA

Downstream 100 bases:

>100_bases
TAAAATTTTAAGCATAAATGTACTTCTTTCCTCATACAGTGAAACTAATAAGGCTTGTCTAAGGAAAGGAATGAAGTAGA
GTGGAAACTTTACCATGGTG

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLKRFKFYMISGIVLVLLIVCNFVDKPIAKVGEVKDTVMMKLNTKESMAQIDGQTIYFKKIGEGKPPLLMLHGFGGSSDG
FSDIYPELARDHTIIAVDILGFGRSSKPIDFEYSFPAQVNLYYKLMKKLGYDQFAVLGHSMGGEMSLNLAYLYPDAVTHL
ILADSTGIESFQQKESYEVPPLSTDLQTVTEITDYNKNEVKNSRDDKGHYDQLTKMRERRIAMEADKIKVPTLIIWGRHD
KSVSWKNGELYHGLFANSTFHIIEKGYHAPFRQEPIEFMEYVQAFFAKNPQ

Sequences:

>Translated_291_residues
MLKRFKFYMISGIVLVLLIVCNFVDKPIAKVGEVKDTVMMKLNTKESMAQIDGQTIYFKKIGEGKPPLLMLHGFGGSSDG
FSDIYPELARDHTIIAVDILGFGRSSKPIDFEYSFPAQVNLYYKLMKKLGYDQFAVLGHSMGGEMSLNLAYLYPDAVTHL
ILADSTGIESFQQKESYEVPPLSTDLQTVTEITDYNKNEVKNSRDDKGHYDQLTKMRERRIAMEADKIKVPTLIIWGRHD
KSVSWKNGELYHGLFANSTFHIIEKGYHAPFRQEPIEFMEYVQAFFAKNPQ
>Mature_291_residues
MLKRFKFYMISGIVLVLLIVCNFVDKPIAKVGEVKDTVMMKLNTKESMAQIDGQTIYFKKIGEGKPPLLMLHGFGGSSDG
FSDIYPELARDHTIIAVDILGFGRSSKPIDFEYSFPAQVNLYYKLMKKLGYDQFAVLGHSMGGEMSLNLAYLYPDAVTHL
ILADSTGIESFQQKESYEVPPLSTDLQTVTEITDYNKNEVKNSRDDKGHYDQLTKMRERRIAMEADKIKVPTLIIWGRHD
KSVSWKNGELYHGLFANSTFHIIEKGYHAPFRQEPIEFMEYVQAFFAKNPQ

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI50658087, Length=106, Percent_Identity=36.7924528301887, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI87081721, Length=278, Percent_Identity=23.021582733813, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558492, Length=112, Percent_Identity=33.0357142857143, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32566936, Length=128, Percent_Identity=31.25, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324392, Length=119, Percent_Identity=30.2521008403361, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33221; Mature: 33221

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRFKFYMISGIVLVLLIVCNFVDKPIAKVGEVKDTVMMKLNTKESMAQIDGQTIYFKK
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEECCHHHHHHCCCCEEEEEE
IGEGKPPLLMLHGFGGSSDGFSDIYPELARDHTIIAVDILGFGRSSKPIDFEYSFPAQVN
CCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEECCCCHHH
LYYKLMKKLGYDQFAVLGHSMGGEMSLNLAYLYPDAVTHLILADSTGIESFQQKESYEVP
HHHHHHHHCCCCCEEHHHHCCCCCEEEEEEEECHHHHHHEEEECCCCHHHHHHHCCCCCC
PLSTDLQTVTEITDYNKNEVKNSRDDKGHYDQLTKMRERRIAMEADKIKVPTLIIWGRHD
CCCCHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCEEEEECCCC
KSVSWKNGELYHGLFANSTFHIIEKGYHAPFRQEPIEFMEYVQAFFAKNPQ
CCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKRFKFYMISGIVLVLLIVCNFVDKPIAKVGEVKDTVMMKLNTKESMAQIDGQTIYFKK
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEEECCHHHHHHCCCCEEEEEE
IGEGKPPLLMLHGFGGSSDGFSDIYPELARDHTIIAVDILGFGRSSKPIDFEYSFPAQVN
CCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEECCCCHHH
LYYKLMKKLGYDQFAVLGHSMGGEMSLNLAYLYPDAVTHLILADSTGIESFQQKESYEVP
HHHHHHHHCCCCCEEHHHHCCCCCEEEEEEEECHHHHHHEEEECCCCHHHHHHHCCCCCC
PLSTDLQTVTEITDYNKNEVKNSRDDKGHYDQLTKMRERRIAMEADKIKVPTLIIWGRHD
CCCCHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCEEEEECCCC
KSVSWKNGELYHGLFANSTFHIIEKGYHAPFRQEPIEFMEYVQAFFAKNPQ
CCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]