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The map label for this gene is thiD [H]

Identifier: 49183724

GI number: 49183724

Start: 759271

End: 760083

Strand: Direct

Name: thiD [H]

Synonym: BAS0700

Alternate gene names: 49183724

Gene position: 759271-760083 (Clockwise)

Preceding gene: 49183723

Following gene: 49183725

Centisome position: 14.52

GC content: 38.38

Gene sequence:

>813_bases
ATGAAAGTAAATAAAGCTTTAACAATTGCAGGATCTGATAGCGGTGGCGGTGCCGGCATTCAAGCAGATTTAAAAACATT
TCAAGAGCTTGGCGTGTATGGGATGACGGCTATTACGGCAATTACTGCTCAAAATACGCTTGGCGTTCAAGGGGTATATC
CAGTTTCGTTAGAAGGTATTACGGAACAGTTGAATTCAATTGGTACGGATTTAACACCAGATGCTGTAAAAATAGGTATG
TTATTTAGCAGCGAAATTATTCAAATTGTAGCAGAATATATTAATAAATTTGGCTGGAATAATATTGTACTAGACCCTGT
TATGATTGCTAAGGGCGGAGCATCATTATTACAACAAGAAGCAGTACAAGCATTAAAAGAATATTTATTACCGGTAGCAA
CTGTTATCACACCGAATGTTCCAGAAGCAGAAGTGTTAACGGGGCTGGAAATTCATAACATTGAAGATAGTAAAGAAGCT
GCAAAAGTACTGCATGAATTAGGTGCGAAATATGTACTTATGAAGGGCGGACATGCAGAATATCAAGGTAATGAAGTAAT
TGATTTACTCTTTGATGGAGAAAAGTTTATTGAATTTAGAAGTGAGCGAATTCCTTCAAAGCAAACACACGGAAGCGGCT
GTACATTTGCTTCAGCAGTTACAGCAGGGCTTGCGAAAGGCTACTCGATGGAAGAGGCAGTTCAAGAGGCAAAACGATTT
ATTAGTATAGCAATTGAAGAGCCGTTGCATATTGGAAGTGGTCATGGACCGACGAATCATTTTGCATACAAAATGAATAA
AGCACAAGTGTAA

Upstream 100 bases:

>100_bases
TGCTTTTAAAGATGGTCGCGTACTTGTACATGGAACGAAAGATATAAGTGAAGCAAAAACAGTTTATCATCGTTATTTTG
GATAGAAAAGGATGAGTGGG

Downstream 100 bases:

>100_bases
AGAAAAGAAAGTCAGTTTTATAACTGGCTTTTTTCTGTTTACTTTTTCCAAATACAGGAGTAATATATCAGCCAGAATAT
ATTTTTTGTAATACGCTGAG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKVNKALTIAGSDSGGGAGIQADLKTFQELGVYGMTAITAITAQNTLGVQGVYPVSLEGITEQLNSIGTDLTPDAVKIGM
LFSSEIIQIVAEYINKFGWNNIVLDPVMIAKGGASLLQQEAVQALKEYLLPVATVITPNVPEAEVLTGLEIHNIEDSKEA
AKVLHELGAKYVLMKGGHAEYQGNEVIDLLFDGEKFIEFRSERIPSKQTHGSGCTFASAVTAGLAKGYSMEEAVQEAKRF
ISIAIEEPLHIGSGHGPTNHFAYKMNKAQV

Sequences:

>Translated_270_residues
MKVNKALTIAGSDSGGGAGIQADLKTFQELGVYGMTAITAITAQNTLGVQGVYPVSLEGITEQLNSIGTDLTPDAVKIGM
LFSSEIIQIVAEYINKFGWNNIVLDPVMIAKGGASLLQQEAVQALKEYLLPVATVITPNVPEAEVLTGLEIHNIEDSKEA
AKVLHELGAKYVLMKGGHAEYQGNEVIDLLFDGEKFIEFRSERIPSKQTHGSGCTFASAVTAGLAKGYSMEEAVQEAKRF
ISIAIEEPLHIGSGHGPTNHFAYKMNKAQV
>Mature_270_residues
MKVNKALTIAGSDSGGGAGIQADLKTFQELGVYGMTAITAITAQNTLGVQGVYPVSLEGITEQLNSIGTDLTPDAVKIGM
LFSSEIIQIVAEYINKFGWNNIVLDPVMIAKGGASLLQQEAVQALKEYLLPVATVITPNVPEAEVLTGLEIHNIEDSKEA
AKVLHELGAKYVLMKGGHAEYQGNEVIDLLFDGEKFIEFRSERIPSKQTHGSGCTFASAVTAGLAKGYSMEEAVQEAKRF
ISIAIEEPLHIGSGHGPTNHFAYKMNKAQV

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=264, Percent_Identity=43.9393939393939, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788758, Length=249, Percent_Identity=27.710843373494, Blast_Score=68, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6325378, Length=272, Percent_Identity=33.0882352941176, Blast_Score=145, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324997, Length=273, Percent_Identity=33.6996336996337, Blast_Score=133, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324517, Length=273, Percent_Identity=34.0659340659341, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28848; Mature: 28848

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNKALTIAGSDSGGGAGIQADLKTFQELGVYGMTAITAITAQNTLGVQGVYPVSLEGI
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHH
TEQLNSIGTDLTPDAVKIGMLFSSEIIQIVAEYINKFGWNNIVLDPVMIAKGGASLLQQE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCHHHHHHH
AVQALKEYLLPVATVITPNVPEAEVLTGLEIHNIEDSKEAAKVLHELGAKYVLMKGGHAE
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEECCCCHHHHHHHHHHCCCCEEEEECCCCC
YQGNEVIDLLFDGEKFIEFRSERIPSKQTHGSGCTFASAVTAGLAKGYSMEEAVQEAKRF
CCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
ISIAIEEPLHIGSGHGPTNHFAYKMNKAQV
HHHHHCCCEECCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MKVNKALTIAGSDSGGGAGIQADLKTFQELGVYGMTAITAITAQNTLGVQGVYPVSLEGI
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHH
TEQLNSIGTDLTPDAVKIGMLFSSEIIQIVAEYINKFGWNNIVLDPVMIAKGGASLLQQE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCHHHHHHH
AVQALKEYLLPVATVITPNVPEAEVLTGLEIHNIEDSKEAAKVLHELGAKYVLMKGGHAE
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEECCCCHHHHHHHHHHCCCCEEEEECCCCC
YQGNEVIDLLFDGEKFIEFRSERIPSKQTHGSGCTFASAVTAGLAKGYSMEEAVQEAKRF
CCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
ISIAIEEPLHIGSGHGPTNHFAYKMNKAQV
HHHHHCCCEECCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]