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The map label for this gene is thiF [H]

Identifier: 49183723

GI number: 49183723

Start: 758236

End: 759255

Strand: Direct

Name: thiF [H]

Synonym: BAS0699

Alternate gene names: 49183723

Gene position: 758236-759255 (Clockwise)

Preceding gene: 49183722

Following gene: 49183724

Centisome position: 14.5

GC content: 37.25

Gene sequence:

>1020_bases
TTGAATAATCGATATTCTCGCCAAGAATTATTTTCTCCGATTGGGGAGGAAGGCCAGCAAAAGATAAGAAAAAAGCATGT
ACTCATTATCGGTGCAGGCGCACTAGGTAGTGCAAATGCAGAAATGTTTGTAAGAGCAGGTGTTGGCACAGTAACGATTG
TTGATCGTGATTATGTCGATTGGAGTAATTTACAAAGGCAGCAATTGTATGTAGAGAGTGATGTGGAAAATAATCTTCCG
AAGGCTGTAGCAGCAAAGAAGCGTCTAGAAGAGATTAATAGTGAAGTAAGAGTAAAAGCGCTCGTTCAAGATGTAACAGC
TGAGGAATTAGAAGAGCTTGTTACAAACGTTAATGTAATGATTGATGCAACTGATAATTTCGAAACGCGTTTCATTGTGA
ATGATATAGCACAAAAATATTCTATTCCATGGATTTACGGAGCATGTGTAGGGAGTTACGGCCTTTCTTACACAATCCTT
CCTAGTAAAACGCCATGTTTATCTTGTTTATTACAATCGATTCCGCTTGGCGGGGCGACATGTGATACAGCGGGGATTAT
ATCGCCTGCTGTATCTCTCGTCGTTTCTCATCAAGTAACGGAAGCTCTTAAACTATTAGTGGAAGATTACGAATCACTTC
GAGATGGACTTGTATCGTTTGATGTATGGAAGAATGAATATTCATGTATGAATGTGCAAAAGCTTCGTAAACATAATTGC
CCTTCGTGCGGAGAGAATGCATTATATCCTTATTTAAACAAAGAAAATACATCGAAAACAGCAGTTTTATGCGGGAGAAA
TACAGTTCAAATTAGACCACCTTATAAAGAGGAAATGGATTTTGAACGATACAAAGAGCTGCTGAATGATCGTGTAAATG
ATTTAAATGTAAATCCATATTTATTATCATTTTCTGTGGAAGAAAAGAGATTAGTTGCTTTTAAAGATGGTCGCGTACTT
GTACATGGAACGAAAGATATAAGTGAAGCAAAAACAGTTTATCATCGTTATTTTGGATAG

Upstream 100 bases:

>100_bases
AAATTAAGTATTGAGGCAGGACGTTTAGGCTTTGAAGCAGGTCGTATTGCACGTAAACGTTGTGCAACAGCAAGTAGTCC
TTTAGAAGGAATGAGCGTAG

Downstream 100 bases:

>100_bases
AAAAGGATGAGTGGGATGAAAGTAAATAAAGCTTTAACAATTGCAGGATCTGATAGCGGTGGCGGTGCCGGCATTCAAGC
AGATTTAAAAACATTTCAAG

Product: thiamine/molybdopterin biosynthesis ThiF/MoeB-like protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNNRYSRQELFSPIGEEGQQKIRKKHVLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVDWSNLQRQQLYVESDVENNLP
KAVAAKKRLEEINSEVRVKALVQDVTAEELEELVTNVNVMIDATDNFETRFIVNDIAQKYSIPWIYGACVGSYGLSYTIL
PSKTPCLSCLLQSIPLGGATCDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPYKEEMDFERYKELLNDRVNDLNVNPYLLSFSVEEKRLVAFKDGRVL
VHGTKDISEAKTVYHRYFG

Sequences:

>Translated_339_residues
MNNRYSRQELFSPIGEEGQQKIRKKHVLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVDWSNLQRQQLYVESDVENNLP
KAVAAKKRLEEINSEVRVKALVQDVTAEELEELVTNVNVMIDATDNFETRFIVNDIAQKYSIPWIYGACVGSYGLSYTIL
PSKTPCLSCLLQSIPLGGATCDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPYKEEMDFERYKELLNDRVNDLNVNPYLLSFSVEEKRLVAFKDGRVL
VHGTKDISEAKTVYHRYFG
>Mature_339_residues
MNNRYSRQELFSPIGEEGQQKIRKKHVLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVDWSNLQRQQLYVESDVENNLP
KAVAAKKRLEEINSEVRVKALVQDVTAEELEELVTNVNVMIDATDNFETRFIVNDIAQKYSIPWIYGACVGSYGLSYTIL
PSKTPCLSCLLQSIPLGGATCDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPYKEEMDFERYKELLNDRVNDLNVNPYLLSFSVEEKRLVAFKDGRVL
VHGTKDISEAKTVYHRYFG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=289, Percent_Identity=28.0276816608997, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI38045948, Length=172, Percent_Identity=29.6511627906977, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI150417996, Length=161, Percent_Identity=29.8136645962733, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=35.3413654618474, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1787048, Length=246, Percent_Identity=30.8943089430894, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17540406, Length=246, Percent_Identity=32.520325203252, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17557754, Length=146, Percent_Identity=31.5068493150685, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=297, Percent_Identity=26.9360269360269, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320598, Length=188, Percent_Identity=30.3191489361702, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24582879, Length=247, Percent_Identity=27.5303643724696, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 38163; Mature: 38163

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRYSRQELFSPIGEEGQQKIRKKHVLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVD
CCCCCCHHHHHHHCCHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCC
WSNLQRQQLYVESDVENNLPKAVAAKKRLEEINSEVRVKALVQDVTAEELEELVTNVNVM
HHHCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IDATDNFETRFIVNDIAQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGAT
EECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCC
CDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCCCCHHHHHHCCC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPYKEEMDFERYKELLNDRVNDLNVNPY
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCHHHCCHHHHHHHHHCCCCCCCCCCE
LLSFSVEEKRLVAFKDGRVLVHGTKDISEAKTVYHRYFG
EEEEECCCCEEEEEECCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNNRYSRQELFSPIGEEGQQKIRKKHVLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVD
CCCCCCHHHHHHHCCHHHHHHHHHCEEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCC
WSNLQRQQLYVESDVENNLPKAVAAKKRLEEINSEVRVKALVQDVTAEELEELVTNVNVM
HHHCHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IDATDNFETRFIVNDIAQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGAT
EECCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCC
CDTAGIISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHCCCCCCCCHHHHHHCCC
PSCGENALYPYLNKENTSKTAVLCGRNTVQIRPPYKEEMDFERYKELLNDRVNDLNVNPY
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCHHHCCHHHHHHHHHCCCCCCCCCCE
LLSFSVEEKRLVAFKDGRVLVHGTKDISEAKTVYHRYFG
EEEEECCCCEEEEEECCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]