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The map label for this gene is ytsJ [H]

Identifier: 49183574

GI number: 49183574

Start: 588527

End: 589726

Strand: Direct

Name: ytsJ [H]

Synonym: BAS0548

Alternate gene names: 49183574

Gene position: 588527-589726 (Clockwise)

Preceding gene: 49183573

Following gene: 49183577

Centisome position: 11.26

GC content: 39.08

Gene sequence:

>1200_bases
ATGTTAGCAAATCAAATTAATGAAAGATCATTGTTATTGCATAAAGAATTAGTAGGGAAAATTGAAATTACAAGTAAAGT
GGAGGTAAATTCAGCGGATGATTTAAGCCTGACGTATACACCAGGTGTAGCGGAGTCGTGTAAAGCGATTGCGGCAGATG
AAGAAACAGTGTATGACTATACAGCACGTGGGAATATGGTGGCAGTTGTCTCAGATGGAACAGCGGTACTTGGTTTAGGG
AACATTGGACCAAAAGCAGCTATGCCTGTTATGGAAGGAAAAAGTATATTATTTAAAAAATTTGCGAATGTAGATGCTTT
CCCGTTATGTTTAGGGACGACGGATGTGGATGAAATCGTTACCCTTGTAAAAAATTTAGAGCCTACATTTGCAGGGATTA
ATTTAGAAGATATTGCAGCACCGCGTTGTTTTGAAATTGAAAAACGATTAAAAGAAGAAACGAATATTCCTGTATTTCAT
GATGATCAACATGGAACAGCTATCGTCGTCTTAGCAGCTGTTATTAATGCATTAAAAGTTGTTAGCAAACAAATGGATCA
TGTGAAAATTGTTATTAACGGTGCGGGTTCGGCAGGAATTGCAATTGGAAAATTATTATTAAAGGCTGGTGCACAGCACA
TTACATTAGTTAGCTTAGAAGGTATTGTTTGTGAAGGTGAAGCATGGATGAACGAAGCACAAATTGAAGTTTCAAAAAAG
ACAAATCGAGAACATGTGCGTGGAACATTAAAAGAAGCGATTCATCAGGCTGATATCTTTATCGGTGTATCTGCCCCTAA
CGTATTAACGAAAGAACTTGTACAGATGATGAATGAGAAAGCAATTGTGTTTGCAATGGCGAATCCAATTCCAGAAATCT
TCCCTGAGGATGCATTAGAGGCTGGGGCAGCTGTAGTAGGAACCGGGCGCTCGGATTATGCGAATCAAGTAAATAATGTA
TTGGCGTTCCCTGGAATATTCCGAGGTGCGTTAGATGTGCGCGCGACTGATATTACAGAAGAAATGAAATTAGCGGCAGC
ATATGGGATCGCTAACATCATTACGGACGAAGAACGTAATGCGAATTATGTAATCCCGAATCCATTAGATAAAAGAGTTG
TTCCAAGTGTTGCGGAAGCTGTAGCGAAAGCAGCCATTGATTCAGGTGTAGCGCAAATTATGAAAATACCAAGTTATTAA

Upstream 100 bases:

>100_bases
TGGTTGTAACGGCGACAATATTATTAAAAATGTTTTCATGATCAAACAAGCTAGCTATATTCGTATAGCTAGCTTGTCAA
ATTAAGGGGAGATTATAAGT

Downstream 100 bases:

>100_bases
TAGCTAAAAGCAGTGCCTAAAAGGCACTGCTTTTTTGAGATAAGAAATCAAATAATGAAGAAGTGTAAAATTATTTATTT
ATGTTTCTTCACTATTTATT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MLANQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDYTARGNMVAVVSDGTAVLGLG
NIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVSKQMDHVKIVINGAGSAGIAIGKLLLKAGAQHITLVSLEGIVCEGEAWMNEAQIEVSKK
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQMMNEKAIVFAMANPIPEIFPEDALEAGAAVVGTGRSDYANQVNNV
LAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERNANYVIPNPLDKRVVPSVAEAVAKAAIDSGVAQIMKIPSY

Sequences:

>Translated_399_residues
MLANQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDYTARGNMVAVVSDGTAVLGLG
NIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVSKQMDHVKIVINGAGSAGIAIGKLLLKAGAQHITLVSLEGIVCEGEAWMNEAQIEVSKK
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQMMNEKAIVFAMANPIPEIFPEDALEAGAAVVGTGRSDYANQVNNV
LAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERNANYVIPNPLDKRVVPSVAEAVAKAAIDSGVAQIMKIPSY
>Mature_399_residues
MLANQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDYTARGNMVAVVSDGTAVLGLG
NIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVSKQMDHVKIVINGAGSAGIAIGKLLLKAGAQHITLVSLEGIVCEGEAWMNEAQIEVSKK
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQMMNEKAIVFAMANPIPEIFPEDALEAGAAVVGTGRSDYANQVNNV
LAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERNANYVIPNPLDKRVVPSVAEAVAKAAIDSGVAQIMKIPSY

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=449, Percent_Identity=24.0534521158129, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4505145, Length=359, Percent_Identity=24.7910863509749, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI62420882, Length=422, Percent_Identity=22.7488151658768, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI62420880, Length=422, Percent_Identity=22.7488151658768, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI239049447, Length=422, Percent_Identity=22.7488151658768, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788806, Length=408, Percent_Identity=48.2843137254902, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI87081919, Length=446, Percent_Identity=26.2331838565022, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322823, Length=312, Percent_Identity=27.8846153846154, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI78707240, Length=446, Percent_Identity=25.5605381165919, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI78707242, Length=439, Percent_Identity=25.7403189066059, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI78707238, Length=439, Percent_Identity=25.7403189066059, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281362672, Length=450, Percent_Identity=25.1111111111111, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356279, Length=450, Percent_Identity=25.1111111111111, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281362674, Length=450, Percent_Identity=25.1111111111111, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24646388, Length=448, Percent_Identity=23.6607142857143, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24646386, Length=453, Percent_Identity=23.6203090507726, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42654; Mature: 42654

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLANQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDY
CCCCCCCCHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCEEEE
TARGNMVAVVSDGTAVLGLGNIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIV
ECCCCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCCEEEEECCCCHHHHH
TLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFHDDQHGTAIVVLAAVINALKV
HHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
VSKQMDHVKIVINGAGSAGIAIGKLLLKAGAQHITLVSLEGIVCEGEAWMNEAQIEVSKK
HHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCEEECCHHHCCCCEEEEEHH
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQMMNEKAIVFAMANPIPEIFPEDALE
CCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHH
AGAAVVGTGRSDYANQVNNVLAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERN
CCCEEEECCCHHHHHHHHCEEECCHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCCC
ANYVIPNPLDKRVVPSVAEAVAKAAIDSGVAQIMKIPSY
CCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLANQINERSLLLHKELVGKIEITSKVEVNSADDLSLTYTPGVAESCKAIAADEETVYDY
CCCCCCCCHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCEEEE
TARGNMVAVVSDGTAVLGLGNIGPKAAMPVMEGKSILFKKFANVDAFPLCLGTTDVDEIV
ECCCCEEEEEECCEEEEEECCCCCHHCCCHHCCCHHHHHHHCCCCCEEEEECCCCHHHHH
TLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFHDDQHGTAIVVLAAVINALKV
HHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
VSKQMDHVKIVINGAGSAGIAIGKLLLKAGAQHITLVSLEGIVCEGEAWMNEAQIEVSKK
HHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCEEECCHHHCCCCEEEEEHH
TNREHVRGTLKEAIHQADIFIGVSAPNVLTKELVQMMNEKAIVFAMANPIPEIFPEDALE
CCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHH
AGAAVVGTGRSDYANQVNNVLAFPGIFRGALDVRATDITEEMKLAAAYGIANIITDEERN
CCCEEEECCCHHHHHHHHCEEECCHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCCC
ANYVIPNPLDKRVVPSVAEAVAKAAIDSGVAQIMKIPSY
CCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]