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The map label for this gene is rumA [C]

Identifier: 46447632

GI number: 46447632

Start: 2380126

End: 2381277

Strand: Direct

Name: rumA [C]

Synonym: pc1998

Alternate gene names: 46447632

Gene position: 2380126-2381277 (Clockwise)

Preceding gene: 46447629

Following gene: 46447633

Centisome position: 98.58

GC content: 36.72

Gene sequence:

>1152_bases
ATGTTATTTCCCATTTCCAAACAAGCACTTTCTTGCCCTCATTTTGATTCGTGTGCAGGGTGCTCTCATCAGCTTTTTGT
TGAACCACCCATTTGGCGTGAAGTGATTGAAAATTTTAAAGAAACATTAAATCCTATTTTGCATCAAGGAACATTAACAG
GTTGGAGATGTCGGGCGAAATTAGCTGTGAGAGGAACAAAACAAAATCCTATCATAGGATTATTTAAAAAAGGGTCTCAC
GAAGCGCTAAATATCCCATTTTGTTTAGTTCATCACCCTCGCATTAATCAAGCGGTTACTATGATTCAAAACTGGATGGA
AAGACATCAATTAAATCCTTATCAAGAGCATTCACAAGCCGGAGATTTGCGCTACTTACAATTTGTTGTAGAGCGAGCAA
GTGGAAAAGTTCAAATAGTGTTTGTTTTAAATTTTAAAGATTTTTCTTCGCCAGAATCCCAAATATGGCGCAAGCTTCTC
CTAGAATTGGCTCAGGAAACGACAAATGCGTTTTGGCATTCTATTTGGGTAAATTTTAATCCCCATGCTACGAACACAAT
TTTTACAGACAAATGGGAGAAAGTGTGGGGTGAGCCTTATTTGTGGGAAAGTTTTGATGGTGTCAAAATTTGTTTTCAAC
CCTCTAATTTCGCTCAAGCTAACCTTGATCTTTTTGAAAAGCTGTTAAAAAAAGTAAAATCATGGGTTCGGGAGAAGGCT
AAAGTTGTCGAGTTTTTTGCAGGAGTGGGAACAATTGGGTTGTCTGTTGCCGCAAAATGTTCTTGGATTAAATGTGAAGA
AATCAATCCTCACTCCAAAGAGTGCTTTTACTACGCAAAGCAACAATTATCTAGTGAAATTAGCCAAAAAATTATGTTTT
ATACAGGATCTGCGGATGAAAATTTAAATCTACTACAAGGTGCTGATACTGTGATTGTAGACCCTCCAAGAAAAGGATTG
AGTCGAAAGTTTATCGAAGGTTTAACGCAATCTTCTGTCGATCAATTGATCTATGTAAGTTGTGGATGGGATTCTTTTAA
AAAAAATAAGGAGACACTTATTTCGCAAGGATGGAATCTAAAAAAATTAGAGGGGTATAGCCTTTTTCCTGGTAGCGAAC
ATATTGAATTATTGACTCTTTTTGAGAGATGA

Upstream 100 bases:

>100_bases
TATTTTTTCATTCCTCCTTTCGTAGATAAGGTCGGATGATAATGAAAGTTTTTAGTGTTTCTAATCATCAGTATAACTAT
TTATTTTAAAGAAAAAATTC

Downstream 100 bases:

>100_bases
GTTTTTTGAGATAAGCAAACATGGATTCAGTGAAATTTTAAAAAATTTATTAAATGTTGATTGAAATGACAACCAAAGTT
TGATACAAATCGATAAAACG

Product: putative 23S rRNA (Uracil-5-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MLFPISKQALSCPHFDSCAGCSHQLFVEPPIWREVIENFKETLNPILHQGTLTGWRCRAKLAVRGTKQNPIIGLFKKGSH
EALNIPFCLVHHPRINQAVTMIQNWMERHQLNPYQEHSQAGDLRYLQFVVERASGKVQIVFVLNFKDFSSPESQIWRKLL
LELAQETTNAFWHSIWVNFNPHATNTIFTDKWEKVWGEPYLWESFDGVKICFQPSNFAQANLDLFEKLLKKVKSWVREKA
KVVEFFAGVGTIGLSVAAKCSWIKCEEINPHSKECFYYAKQQLSSEISQKIMFYTGSADENLNLLQGADTVIVDPPRKGL
SRKFIEGLTQSSVDQLIYVSCGWDSFKKNKETLISQGWNLKKLEGYSLFPGSEHIELLTLFER

Sequences:

>Translated_383_residues
MLFPISKQALSCPHFDSCAGCSHQLFVEPPIWREVIENFKETLNPILHQGTLTGWRCRAKLAVRGTKQNPIIGLFKKGSH
EALNIPFCLVHHPRINQAVTMIQNWMERHQLNPYQEHSQAGDLRYLQFVVERASGKVQIVFVLNFKDFSSPESQIWRKLL
LELAQETTNAFWHSIWVNFNPHATNTIFTDKWEKVWGEPYLWESFDGVKICFQPSNFAQANLDLFEKLLKKVKSWVREKA
KVVEFFAGVGTIGLSVAAKCSWIKCEEINPHSKECFYYAKQQLSSEISQKIMFYTGSADENLNLLQGADTVIVDPPRKGL
SRKFIEGLTQSSVDQLIYVSCGWDSFKKNKETLISQGWNLKKLEGYSLFPGSEHIELLTLFER
>Mature_383_residues
MLFPISKQALSCPHFDSCAGCSHQLFVEPPIWREVIENFKETLNPILHQGTLTGWRCRAKLAVRGTKQNPIIGLFKKGSH
EALNIPFCLVHHPRINQAVTMIQNWMERHQLNPYQEHSQAGDLRYLQFVVERASGKVQIVFVLNFKDFSSPESQIWRKLL
LELAQETTNAFWHSIWVNFNPHATNTIFTDKWEKVWGEPYLWESFDGVKICFQPSNFAQANLDLFEKLLKKVKSWVREKA
KVVEFFAGVGTIGLSVAAKCSWIKCEEINPHSKECFYYAKQQLSSEISQKIMFYTGSADENLNLLQGADTVIVDPPRKGL
SRKFIEGLTQSSVDQLIYVSCGWDSFKKNKETLISQGWNLKKLEGYSLFPGSEHIELLTLFER

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family

Homologues:

Organism=Escherichia coli, GI1787083, Length=337, Percent_Identity=23.7388724035608, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789148, Length=390, Percent_Identity=22.3076923076923, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24666579, Length=370, Percent_Identity=21.6216216216216, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1998_PARUW (Q6M9M7)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008997.1
- ProteinModelPortal:   Q6M9M7
- STRING:   Q6M9M7
- GeneID:   2779575
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1998
- NMPDR:   fig|264201.1.peg.1998
- eggNOG:   COG2265
- OMA:   QANTRAF
- PhylomeDB:   Q6M9M7
- BioCyc:   CPRO264201:PC1998-MONOMER
- InterPro:   IPR010280

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: 2.1.1.- [C]

Molecular weight: Translated: 44079; Mature: 44079

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 341-341 BINDING 219-219 BINDING 246-246 BINDING 267-267 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFPISKQALSCPHFDSCAGCSHQLFVEPPIWREVIENFKETLNPILHQGTLTGWRCRAK
CCCCCCHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LAVRGTKQNPIIGLFKKGSHEALNIPFCLVHHPRINQAVTMIQNWMERHQLNPYQEHSQA
EEEECCCCCCEEEEEECCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCC
GDLRYLQFVVERASGKVQIVFVLNFKDFSSPESQIWRKLLLELAQETTNAFWHSIWVNFN
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEC
PHATNTIFTDKWEKVWGEPYLWESFDGVKICFQPSNFAQANLDLFEKLLKKVKSWVREKA
CCCCCCEECCCHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
KVVEFFAGVGTIGLSVAAKCSWIKCEEINPHSKECFYYAKQQLSSEISQKIMFYTGSADE
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCC
NLNLLQGADTVIVDPPRKGLSRKFIEGLTQSSVDQLIYVSCGWDSFKKNKETLISQGWNL
CCEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHCHHHHHHHCCCCE
KKLEGYSLFPGSEHIELLTLFER
EEECCCEECCCCCCEEEEEEECC
>Mature Secondary Structure
MLFPISKQALSCPHFDSCAGCSHQLFVEPPIWREVIENFKETLNPILHQGTLTGWRCRAK
CCCCCCHHHCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
LAVRGTKQNPIIGLFKKGSHEALNIPFCLVHHPRINQAVTMIQNWMERHQLNPYQEHSQA
EEEECCCCCCEEEEEECCCCCEECCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCC
GDLRYLQFVVERASGKVQIVFVLNFKDFSSPESQIWRKLLLELAQETTNAFWHSIWVNFN
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEC
PHATNTIFTDKWEKVWGEPYLWESFDGVKICFQPSNFAQANLDLFEKLLKKVKSWVREKA
CCCCCCEECCCHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
KVVEFFAGVGTIGLSVAAKCSWIKCEEINPHSKECFYYAKQQLSSEISQKIMFYTGSADE
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCC
NLNLLQGADTVIVDPPRKGLSRKFIEGLTQSSVDQLIYVSCGWDSFKKNKETLISQGWNL
CCEEECCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHCHHHHHHHCCCCE
KKLEGYSLFPGSEHIELLTLFER
EEECCCEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA