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The map label for this gene is recA [H]

Identifier: 46447629

GI number: 46447629

Start: 2376732

End: 2377817

Strand: Direct

Name: recA [H]

Synonym: pc1995

Alternate gene names: 46447629

Gene position: 2376732-2377817 (Clockwise)

Preceding gene: 46447628

Following gene: 46447632

Centisome position: 98.44

GC content: 38.86

Gene sequence:

>1086_bases
ATGTCTCAATCAGCCCCTCCAGATTCTGAACGTAAAAAAGCACTTGGCCTGGCCGTTTCTCAAATTAAAAAACAATTTGG
TGAAGGTGCTATTATGTCTTTTGGCAAGCACTCTTCTGAAAAAGAAATGAGTGTGATTAAAACAGGTGCTCTTGCTCTGG
ATATCGCCTTAGGAATTGGCGGTGTTCCACGTGGACGCGTTGTAGAAATTTATGGACCTGAATCATCTGGTAAGTCAACT
TTAGCTCTTCATATCGTAGCTAATGCTCAAAAAAATGGGGGGTTAGCTGCTTATATTGATGCGGAACATGCCCTTGACCC
TAGTTATGCTGGTAAAATTGGTATTAATATAGATGATTTGATGATTTCACAACCTGATAGTGGGGAAGAAGCTTTAAACA
TTGCAGAGATGCTTGCACGTTCTAACGCTGTAGACGTGATTGTCATTGACTCTGTTGCCGCTCTCGTTCCTAAATCTGAA
CTTGAGGGAGAAATTGGAGATCAATTCATGGGTCTTCAAGCTCGCATGATGTCACAAGCATTAAGAAAATTAACGGCCTC
TCTCTCAAAAAGCAATACATGTGCTATTTTTATTAACCAAATTCGAGATAAAATAGGGATCGTTTATGGAAATCCAGAAA
CGACAACAGGGGGACGTGCCTTAAAATTTTACTCTTCGGTACGATTAGATATTCGTCGTACAGGAGGAATTAAAGGACCG
GACAATACTGAAATTGGTAATCGGGTAAAAGTAAAAGTTAGTAAAAATAAAATGGCTCCTCCTTTTCAAATTGCTGAATT
TGATATCTTATTTAATGAAGGTATTTCTCGTACAGGTTCTGCGATTGATATGGCAACAGAGTATAATATTGTTGATAAAA
AAGGGGCCTGGTTTAGCTACAAAGGGCAACGCTTAGGTCAAGGAAGAGAAGCGGTCAGAGAAGAGTTTAAAAATAATCCA
ACGCTTTTAGAAGAAATAGAAGCGCTAATTTTACAACAGTATAAAGAAAATCGTTCCAAGCCCGCATCCAAAAAAACTTC
TATCGGTGCTGCAATAGAAGAAATTGAAGGGGCATTGAACGAATAA

Upstream 100 bases:

>100_bases
TTTTGATGTGTCATATAATGAAGTTACTTTTACAAAAGATGTTAGTAATCTCAACTTTCGTTAATAGTTAACTCAAAAAA
GTTTATTCAAAGGAGTCTTT

Downstream 100 bases:

>100_bases
TTCGTTCTATCGATGTTGGATAAAGCTAATTCTTTATTCAGCATCGATTTAAAGGCTATACTTTGCGTACCTCATGTATA
CACAGCCTTTTCTATCATTT

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSQSAPPDSERKKALGLAVSQIKKQFGEGAIMSFGKHSSEKEMSVIKTGALALDIALGIGGVPRGRVVEIYGPESSGKST
LALHIVANAQKNGGLAAYIDAEHALDPSYAGKIGINIDDLMISQPDSGEEALNIAEMLARSNAVDVIVIDSVAALVPKSE
LEGEIGDQFMGLQARMMSQALRKLTASLSKSNTCAIFINQIRDKIGIVYGNPETTTGGRALKFYSSVRLDIRRTGGIKGP
DNTEIGNRVKVKVSKNKMAPPFQIAEFDILFNEGISRTGSAIDMATEYNIVDKKGAWFSYKGQRLGQGREAVREEFKNNP
TLLEEIEALILQQYKENRSKPASKKTSIGAAIEEIEGALNE

Sequences:

>Translated_361_residues
MSQSAPPDSERKKALGLAVSQIKKQFGEGAIMSFGKHSSEKEMSVIKTGALALDIALGIGGVPRGRVVEIYGPESSGKST
LALHIVANAQKNGGLAAYIDAEHALDPSYAGKIGINIDDLMISQPDSGEEALNIAEMLARSNAVDVIVIDSVAALVPKSE
LEGEIGDQFMGLQARMMSQALRKLTASLSKSNTCAIFINQIRDKIGIVYGNPETTTGGRALKFYSSVRLDIRRTGGIKGP
DNTEIGNRVKVKVSKNKMAPPFQIAEFDILFNEGISRTGSAIDMATEYNIVDKKGAWFSYKGQRLGQGREAVREEFKNNP
TLLEEIEALILQQYKENRSKPASKKTSIGAAIEEIEGALNE
>Mature_360_residues
SQSAPPDSERKKALGLAVSQIKKQFGEGAIMSFGKHSSEKEMSVIKTGALALDIALGIGGVPRGRVVEIYGPESSGKSTL
ALHIVANAQKNGGLAAYIDAEHALDPSYAGKIGINIDDLMISQPDSGEEALNIAEMLARSNAVDVIVIDSVAALVPKSEL
EGEIGDQFMGLQARMMSQALRKLTASLSKSNTCAIFINQIRDKIGIVYGNPETTTGGRALKFYSSVRLDIRRTGGIKGPD
NTEIGNRVKVKVSKNKMAPPFQIAEFDILFNEGISRTGSAIDMATEYNIVDKKGAWFSYKGQRLGQGREAVREEFKNNPT
LLEEIEALILQQYKENRSKPASKKTSIGAAIEEIEGALNE

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=320, Percent_Identity=59.0625, Blast_Score=393, Evalue=1e-110,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 38875; Mature: 38744

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSAPPDSERKKALGLAVSQIKKQFGEGAIMSFGKHSSEKEMSVIKTGALALDIALGIG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCEEEEEECCCC
GVPRGRVVEIYGPESSGKSTLALHIVANAQKNGGLAAYIDAEHALDPSYAGKIGINIDDL
CCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEEHHHCCCCCCCCEECCCHHHE
MISQPDSGEEALNIAEMLARSNAVDVIVIDSVAALVPKSELEGEIGDQFMGLQARMMSQA
EEECCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHCCHHHCCCCHHHHHHHHHHHHHHHH
LRKLTASLSKSNTCAIFINQIRDKIGIVYGNPETTTGGRALKFYSSVRLDIRRTGGIKGP
HHHHHHHHCCCCEEEEEHHHHHHHEEEEECCCCCCCCCCEEEEHHHHEEEEEECCCCCCC
DNTEIGNRVKVKVSKNKMAPPFQIAEFDILFNEGISRTGSAIDMATEYNIVDKKGAWFSY
CCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCEEEEHCCCCEECCCCCEEEE
KGQRLGQGREAVREEFKNNPTLLEEIEALILQQYKENRSKPASKKTSIGAAIEEIEGALN
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
E
C
>Mature Secondary Structure 
SQSAPPDSERKKALGLAVSQIKKQFGEGAIMSFGKHSSEKEMSVIKTGALALDIALGIG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCEEEEEECCCC
GVPRGRVVEIYGPESSGKSTLALHIVANAQKNGGLAAYIDAEHALDPSYAGKIGINIDDL
CCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEEHHHCCCCCCCCEECCCHHHE
MISQPDSGEEALNIAEMLARSNAVDVIVIDSVAALVPKSELEGEIGDQFMGLQARMMSQA
EEECCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHCCHHHCCCCHHHHHHHHHHHHHHHH
LRKLTASLSKSNTCAIFINQIRDKIGIVYGNPETTTGGRALKFYSSVRLDIRRTGGIKGP
HHHHHHHHCCCCEEEEEHHHHHHHEEEEECCCCCCCCCCEEEEHHHHEEEEEECCCCCCC
DNTEIGNRVKVKVSKNKMAPPFQIAEFDILFNEGISRTGSAIDMATEYNIVDKKGAWFSY
CCCCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCEEEEHCCCCEECCCCCEEEE
KGQRLGQGREAVREEFKNNPTLLEEIEALILQQYKENRSKPASKKTSIGAAIEEIEGALN
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]