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The map label for this gene is dnaB [H]

Identifier: 46447444

GI number: 46447444

Start: 2162991

End: 2164436

Strand: Direct

Name: dnaB [H]

Synonym: pc1810

Alternate gene names: 46447444

Gene position: 2162991-2164436 (Clockwise)

Preceding gene: 46447441

Following gene: 46447445

Centisome position: 89.58

GC content: 37.21

Gene sequence:

>1446_bases
ATGACAGAAGCTAACGTTAAAGTTAAAATTGCTCCACACTCTAAAGAATCAGAAATGATGGTTATTGGCTGCATGCTGAC
AAGCATCAATAGTCTGAATATTGCATCTGATTCTCTTGATGACTCTGACTTTTATTTTAATGAACACAAAATCATTTTTC
AAATTCTTAAAACTGCTTATAAAAAAGATAAGCCGGCCGATATTCATCTTGTCGCCGAGGAACTCAAACGTGTAGAAAAA
CTAAGTGCTGTCGGTGGCGTTTCTTATTTAGCTACTTTAGCTCAATATGCCGGAACGTCGGCTTTTATTGAAGAGTACGT
TGAACTTGTCAAAAATAAATCGATTCTCCGACGCATGATTCATGCTGCTCAGATAATTGAAAAAAGTGCTTTAGAAGAAT
CACAAGATGTTCAAGTTGCTTTAGATGAAGCTCAACAACTTTTTTTTCAAATTGGCCAAACCTCTAATCCTACATCAGGA
CTCATTCTCCAAGATATTTTTTCCGGATTAAAATCTGAATCGGGACTTCCTTATTTAAAAGAGTTACAAGAAAAACAAGA
GAGGTACGCTCAACGAGGGCCAGAAGATTCGGGAATTAGTGGAATCCCCTCTCATTTTATTGATTTAGATAAAATGATTA
ATGGTTTAAATAACTCAAATTTAATGATTTTAGCCGCCCGCCCAGCAATGGGAAAAACTTCTTTTGCCATCAATATTGCA
GAAAATGTGTGTTTTAAAAATAAAATTCCTGTCGGTATCTTCTCCTTAGAAATGTCAGCTGAACAATTAGTTCATCGCAT
TGTTTGTTCACAAGCAGAAGTCCAATCCGATAAAATTAAAACAGGTGCTTTAGATGGAGTGGAATTTCAACGCATTGTTA
GCTGCGTCAATGACATGCAAAACCATGTGATGATCATTGATGATCAAGCAGGTTTAAAAATTACCGACTTAAGAGCTCGT
GCCCGAAGAATGAAAGAAAGTCACGGTATTGGGCTTCTGATTATTGATTACCTTCAACTGCTTTCGGGATCGGGTTCTAA
TCGCACCTCAGAGAATCGACAAAACGAAATTTCAGAAATTTCCCGTATGCTCAAGAACTTAGCACGCGAATTGAACATCC
CTATTATTTGTTTGTCTCAATTATCAAGAAAAGTGGAAGAACGTCCTGGACACCGCCCCATGATGAGTGACTTAAGAGAA
AGTGGAAGTATCGAGCAAGATTCTGATATTGTGATGTTTCTCTTAAGACGCGAATACTACGATCCTAACGACAAACCAGG
AATGGCCGAGTTGATTGTCGCTAAAAATAGACATGGAAGTGTAGGTAGTGTCAATTTAACATTTCGAAAAGAATTTGCAC
AATTTGCAAATTACTCTCCTGTTCGCTACGATCCTTCTCAAATTGATTCAGAAACTGAACAAGCATTTAAACATTTTTCA
CCCTAA

Upstream 100 bases:

>100_bases
AACAAAAATTAACTTTCAGAAAAGAAACTTCTTGTCATCATAGATGAAAGAAATTTCTGCTCATACCTATTTATTTTAAA
GATTAACATTAAAAATTGCC

Downstream 100 bases:

>100_bases
ATTTATAAAAGAAATTTACCTGATAAAAGCTTGCATCATAAATCATCTTATTTTAATCTTTTCGCTTCTTTGGAGGGGTG
TCCGAGTGGCTTAAGGAGCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTEANVKVKIAPHSKESEMMVIGCMLTSINSLNIASDSLDDSDFYFNEHKIIFQILKTAYKKDKPADIHLVAEELKRVEK
LSAVGGVSYLATLAQYAGTSAFIEEYVELVKNKSILRRMIHAAQIIEKSALEESQDVQVALDEAQQLFFQIGQTSNPTSG
LILQDIFSGLKSESGLPYLKELQEKQERYAQRGPEDSGISGIPSHFIDLDKMINGLNNSNLMILAARPAMGKTSFAINIA
ENVCFKNKIPVGIFSLEMSAEQLVHRIVCSQAEVQSDKIKTGALDGVEFQRIVSCVNDMQNHVMIIDDQAGLKITDLRAR
ARRMKESHGIGLLIIDYLQLLSGSGSNRTSENRQNEISEISRMLKNLARELNIPIICLSQLSRKVEERPGHRPMMSDLRE
SGSIEQDSDIVMFLLRREYYDPNDKPGMAELIVAKNRHGSVGSVNLTFRKEFAQFANYSPVRYDPSQIDSETEQAFKHFS
P

Sequences:

>Translated_481_residues
MTEANVKVKIAPHSKESEMMVIGCMLTSINSLNIASDSLDDSDFYFNEHKIIFQILKTAYKKDKPADIHLVAEELKRVEK
LSAVGGVSYLATLAQYAGTSAFIEEYVELVKNKSILRRMIHAAQIIEKSALEESQDVQVALDEAQQLFFQIGQTSNPTSG
LILQDIFSGLKSESGLPYLKELQEKQERYAQRGPEDSGISGIPSHFIDLDKMINGLNNSNLMILAARPAMGKTSFAINIA
ENVCFKNKIPVGIFSLEMSAEQLVHRIVCSQAEVQSDKIKTGALDGVEFQRIVSCVNDMQNHVMIIDDQAGLKITDLRAR
ARRMKESHGIGLLIIDYLQLLSGSGSNRTSENRQNEISEISRMLKNLARELNIPIICLSQLSRKVEERPGHRPMMSDLRE
SGSIEQDSDIVMFLLRREYYDPNDKPGMAELIVAKNRHGSVGSVNLTFRKEFAQFANYSPVRYDPSQIDSETEQAFKHFS
P
>Mature_480_residues
TEANVKVKIAPHSKESEMMVIGCMLTSINSLNIASDSLDDSDFYFNEHKIIFQILKTAYKKDKPADIHLVAEELKRVEKL
SAVGGVSYLATLAQYAGTSAFIEEYVELVKNKSILRRMIHAAQIIEKSALEESQDVQVALDEAQQLFFQIGQTSNPTSGL
ILQDIFSGLKSESGLPYLKELQEKQERYAQRGPEDSGISGIPSHFIDLDKMINGLNNSNLMILAARPAMGKTSFAINIAE
NVCFKNKIPVGIFSLEMSAEQLVHRIVCSQAEVQSDKIKTGALDGVEFQRIVSCVNDMQNHVMIIDDQAGLKITDLRARA
RRMKESHGIGLLIIDYLQLLSGSGSNRTSENRQNEISEISRMLKNLARELNIPIICLSQLSRKVEERPGHRPMMSDLRES
GSIEQDSDIVMFLLRREYYDPNDKPGMAELIVAKNRHGSVGSVNLTFRKEFAQFANYSPVRYDPSQIDSETEQAFKHFSP

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=463, Percent_Identity=38.2289416846652, Blast_Score=310, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53882; Mature: 53751

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEANVKVKIAPHSKESEMMVIGCMLTSINSLNIASDSLDDSDFYFNEHKIIFQILKTAY
CCCCCEEEEECCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHH
KKDKPADIHLVAEELKRVEKLSAVGGVSYLATLAQYAGTSAFIEEYVELVKNKSILRRMI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHH
HAAQIIEKSALEESQDVQVALDEAQQLFFQIGQTSNPTSGLILQDIFSGLKSESGLPYLK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
ELQEKQERYAQRGPEDSGISGIPSHFIDLDKMINGLNNSNLMILAARPAMGKTSFAINIA
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEHH
ENVCFKNKIPVGIFSLEMSAEQLVHRIVCSQAEVQSDKIKTGALDGVEFQRIVSCVNDMQ
HHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NHVMIIDDQAGLKITDLRARARRMKESHGIGLLIIDYLQLLSGSGSNRTSENRQNEISEI
CCEEEEECCCCCEEHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SRMLKNLARELNIPIICLSQLSRKVEERPGHRPMMSDLRESGSIEQDSDIVMFLLRREYY
HHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHC
DPNDKPGMAELIVAKNRHGSVGSVNLTFRKEFAQFANYSPVRYDPSQIDSETEQAFKHFS
CCCCCCCHHHHEEECCCCCCCCEEEEHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCC
P
C
>Mature Secondary Structure 
TEANVKVKIAPHSKESEMMVIGCMLTSINSLNIASDSLDDSDFYFNEHKIIFQILKTAY
CCCCEEEEECCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHH
KKDKPADIHLVAEELKRVEKLSAVGGVSYLATLAQYAGTSAFIEEYVELVKNKSILRRMI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHH
HAAQIIEKSALEESQDVQVALDEAQQLFFQIGQTSNPTSGLILQDIFSGLKSESGLPYLK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
ELQEKQERYAQRGPEDSGISGIPSHFIDLDKMINGLNNSNLMILAARPAMGKTSFAINIA
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEHH
ENVCFKNKIPVGIFSLEMSAEQLVHRIVCSQAEVQSDKIKTGALDGVEFQRIVSCVNDMQ
HHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NHVMIIDDQAGLKITDLRARARRMKESHGIGLLIIDYLQLLSGSGSNRTSENRQNEISEI
CCEEEEECCCCCEEHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SRMLKNLARELNIPIICLSQLSRKVEERPGHRPMMSDLRESGSIEQDSDIVMFLLRREYY
HHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHC
DPNDKPGMAELIVAKNRHGSVGSVNLTFRKEFAQFANYSPVRYDPSQIDSETEQAFKHFS
CCCCCCCHHHHEEECCCCCCCCEEEEHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]