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The map label for this gene is rlmH

Identifier: 46447342

GI number: 46447342

Start: 2046649

End: 2047071

Strand: Direct

Name: rlmH

Synonym: pc1708

Alternate gene names: 46447342

Gene position: 2046649-2047071 (Clockwise)

Preceding gene: 46447341

Following gene: 46447344

Centisome position: 84.77

GC content: 33.57

Gene sequence:

>423_bases
ATGCTCAAACTACGAATTTTATCCGTAGGAAAAACAAAAGAAAAATGGCTAGAAGATGCTTTTAATGAATATCAAAAAAG
ACTGAAAGCAAATTTACAAATCGAATGTCTGTGGGCTAAAGATTCTTACCAACTGCTTGAATGGACTCAAAAAGAATCTC
TGATCATTTGTTTAGATCCTACTGGACGTTTATTGACAAGCGAAGCATTTGCTACTTTTTTCAGTAAATGTTGGGAACAA
GGAGGATCACGATTGACAATTGTGATAGGCGGTGCAGAGGGTTTACCTTTAGAGTTGAAACAACATTCCATACTCATTAG
TCTATCTCTTTTAACATTTACCCATCAAATTACTCGCCTTATTTTAATTGAGCAAATTTATCGAGCCACAGAGATTCTTA
AAAATTCACAATATCATAAATAA

Upstream 100 bases:

>100_bases
AACAGTACTAAACTAAATTTGGTTTGTATTTATCTGGAATTTTTTTTTATTTTTTAGGAAATATAGGAAAAATGAATTCA
ACGCCAAACAATGGAAGTTT

Downstream 100 bases:

>100_bases
TTATAAAGAAATTGAATCATTTATAATGATTCTTTAGTTTTTATTAAAATTTAAAACTTTTGATGTTAAATTTATTTTTA
AAAATCAATTAAAATAATTA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MLKLRILSVGKTKEKWLEDAFNEYQKRLKANLQIECLWAKDSYQLLEWTQKESLIICLDPTGRLLTSEAFATFFSKCWEQ
GGSRLTIVIGGAEGLPLELKQHSILISLSLLTFTHQITRLILIEQIYRATEILKNSQYHK

Sequences:

>Translated_140_residues
MLKLRILSVGKTKEKWLEDAFNEYQKRLKANLQIECLWAKDSYQLLEWTQKESLIICLDPTGRLLTSEAFATFFSKCWEQ
GGSRLTIVIGGAEGLPLELKQHSILISLSLLTFTHQITRLILIEQIYRATEILKNSQYHK
>Mature_140_residues
MLKLRILSVGKTKEKWLEDAFNEYQKRLKANLQIECLWAKDSYQLLEWTQKESLIICLDPTGRLLTSEAFATFFSKCWEQ
GGSRLTIVIGGAEGLPLELKQHSILISLSLLTFTHQITRLILIEQIYRATEILKNSQYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=27.5641025641026, Blast_Score=63, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_PARUW (Q6MAG7)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008707.1
- ProteinModelPortal:   Q6MAG7
- SMR:   Q6MAG7
- STRING:   Q6MAG7
- GeneID:   2780066
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1708
- NMPDR:   fig|264201.1.peg.1708
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   NISHYKI
- PhylomeDB:   Q6MAG7
- ProtClustDB:   CLSK2762466
- BioCyc:   CPRO264201:PC1708-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 16227; Mature: 16227

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLRILSVGKTKEKWLEDAFNEYQKRLKANLQIECLWAKDSYQLLEWTQKESLIICLDP
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCEEEEECC
TGRLLTSEAFATFFSKCWEQGGSRLTIVIGGAEGLPLELKQHSILISLSLLTFTHQITRL
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCEEEEEHHHHHHHHHHHHH
ILIEQIYRATEILKNSQYHK
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLKLRILSVGKTKEKWLEDAFNEYQKRLKANLQIECLWAKDSYQLLEWTQKESLIICLDP
CEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCEEEEECC
TGRLLTSEAFATFFSKCWEQGGSRLTIVIGGAEGLPLELKQHSILISLSLLTFTHQITRL
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEECCCEEEEEHHHHHHHHHHHHH
ILIEQIYRATEILKNSQYHK
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA