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The map label for this gene is murB

Identifier: 46447258

GI number: 46447258

Start: 1943549

End: 1944448

Strand: Direct

Name: murB

Synonym: pc1624

Alternate gene names: 46447258

Gene position: 1943549-1944448 (Clockwise)

Preceding gene: 46447257

Following gene: 46447259

Centisome position: 80.5

GC content: 38.22

Gene sequence:

>900_bases
ATGAAATACTCGATTCCCATGAAAATACCTAATCAATATCAAACGAACTGTCTATTAAAAGAGATCACAACATTTGGAAT
TGGGGGCCCCGCTAAGTATTTTGTTGAAGTTCGCACAATTCCCGATATGCAAAAAACCCTTCTATTTTGTTATCAAAATG
AAATTCCGTATTTTATTTTAGGAAAAGGATCAAACTCTCTTTTCGATGACCGAGGATTTAACGGACTTGTCATTGCTAAT
CGAATTGATTGTCTGGAAAAAAATGAAAAAGGTTGTTGGCATGTAGGAGCTGGCTATAGCTTTTCATTATTGGGATCTCA
AACAGCAAGGCAAGGTTGGGAAGGACTGGAGTTTGCGTCTGGTATCCCAGGTAGTGTGGGAGGAGCTATTTTCATGAATG
CTGGTGCGAATGGTCGAGAAACTGCAGACAATTTGATTAGTGTAGATTTTGTAGATGAGCAAGGAAAACTCATTCATTTT
AAACGCTCTAACTTAAACTTTCAGTATCGCACATCACCTTTTCAAAATATTAAAGGCGCTATTGTTTCAGCCACATTTCA
ATTAAACGCCTCTCAAGAAGCTCGTCAAAAACAACTTTCTATTATTGATTATCGAAAAAAAACTCAACCTTACAAAGCAA
AATCTGCAGGGTGTGTATTTCGAAATCCCAACTGTGGTCATGCAGGAGCTTTAATTGAACAAGCAGGTTTAAAAGAAACA
AAAATTGGAGGAGCCGCAGTCTCTTCTGTGCACGCTAATTTTATTATCAATAGTGGTCTGGCAACATCTCAAGACGTCTT
AGCTCTCATTCGCCTCATCCAAGAAACAGTAAAAGCAAAGACTGGTGCGGAACTCGAAAGTGAAATACGCTATGTCCCCT
ATGATGTTAATCAAGGTTAA

Upstream 100 bases:

>100_bases
AATTCAAATCTGCTTGTCGAAAGTTCTCAAATCCTTGAACAAAGCGAACAAGTCGCTGCTGATTTTTCTTTAGAAAGTCC
TCTTAGCAAAGAAAAACATA

Downstream 100 bases:

>100_bases
ACTATGAACAATTTTCGAGCAGATTTACATTGCCACACAACTTGTTCAGATGGAACTGTTGATCCTCTCGAGATCATTAA
TTTAGCAATTAACTCTGGAT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKYSIPMKIPNQYQTNCLLKEITTFGIGGPAKYFVEVRTIPDMQKTLLFCYQNEIPYFILGKGSNSLFDDRGFNGLVIAN
RIDCLEKNEKGCWHVGAGYSFSLLGSQTARQGWEGLEFASGIPGSVGGAIFMNAGANGRETADNLISVDFVDEQGKLIHF
KRSNLNFQYRTSPFQNIKGAIVSATFQLNASQEARQKQLSIIDYRKKTQPYKAKSAGCVFRNPNCGHAGALIEQAGLKET
KIGGAAVSSVHANFIINSGLATSQDVLALIRLIQETVKAKTGAELESEIRYVPYDVNQG

Sequences:

>Translated_299_residues
MKYSIPMKIPNQYQTNCLLKEITTFGIGGPAKYFVEVRTIPDMQKTLLFCYQNEIPYFILGKGSNSLFDDRGFNGLVIAN
RIDCLEKNEKGCWHVGAGYSFSLLGSQTARQGWEGLEFASGIPGSVGGAIFMNAGANGRETADNLISVDFVDEQGKLIHF
KRSNLNFQYRTSPFQNIKGAIVSATFQLNASQEARQKQLSIIDYRKKTQPYKAKSAGCVFRNPNCGHAGALIEQAGLKET
KIGGAAVSSVHANFIINSGLATSQDVLALIRLIQETVKAKTGAELESEIRYVPYDVNQG
>Mature_299_residues
MKYSIPMKIPNQYQTNCLLKEITTFGIGGPAKYFVEVRTIPDMQKTLLFCYQNEIPYFILGKGSNSLFDDRGFNGLVIAN
RIDCLEKNEKGCWHVGAGYSFSLLGSQTARQGWEGLEFASGIPGSVGGAIFMNAGANGRETADNLISVDFVDEQGKLIHF
KRSNLNFQYRTSPFQNIKGAIVSATFQLNASQEARQKQLSIIDYRKKTQPYKAKSAGCVFRNPNCGHAGALIEQAGLKET
KIGGAAVSSVHANFIINSGLATSQDVLALIRLIQETVKAKTGAELESEIRYVPYDVNQG

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=329, Percent_Identity=29.483282674772, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_PARUW (Q6MAQ1)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008623.1
- ProteinModelPortal:   Q6MAQ1
- STRING:   Q6MAQ1
- GeneID:   2781163
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1624
- NMPDR:   fig|264201.1.peg.1624
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   LESSKIP
- PhylomeDB:   Q6MAQ1
- ProtClustDB:   PRK13905
- BioCyc:   CPRO264201:PC1624-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 32784; Mature: 32784

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 170-170 ACT_SITE 218-218 ACT_SITE 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSIPMKIPNQYQTNCLLKEITTFGIGGPAKYFVEVRTIPDMQKTLLFCYQNEIPYFIL
CCEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHEEEEEECCCCEEEE
GKGSNSLFDDRGFNGLVIANRIDCLEKNEKGCWHVGAGYSFSLLGSQTARQGWEGLEFAS
ECCCCCCCCCCCCCCEEEECCHHHHHCCCCCCEEECCCCEEEECCCHHHHCCCCCCHHHC
GIPGSVGGAIFMNAGANGRETADNLISVDFVDEQGKLIHFKRSNLNFQYRTSPFQNIKGA
CCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEECCCHHHCCCE
IVSATFQLNASQEARQKQLSIIDYRKKTQPYKAKSAGCVFRNPNCGHAGALIEQAGLKET
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCCC
KIGGAAVSSVHANFIINSGLATSQDVLALIRLIQETVKAKTGAELESEIRYVPYDVNQG
CCCCHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCC
>Mature Secondary Structure
MKYSIPMKIPNQYQTNCLLKEITTFGIGGPAKYFVEVRTIPDMQKTLLFCYQNEIPYFIL
CCEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHEEEEEECCCCEEEE
GKGSNSLFDDRGFNGLVIANRIDCLEKNEKGCWHVGAGYSFSLLGSQTARQGWEGLEFAS
ECCCCCCCCCCCCCCEEEECCHHHHHCCCCCCEEECCCCEEEECCCHHHHCCCCCCHHHC
GIPGSVGGAIFMNAGANGRETADNLISVDFVDEQGKLIHFKRSNLNFQYRTSPFQNIKGA
CCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEECCCHHHCCCE
IVSATFQLNASQEARQKQLSIIDYRKKTQPYKAKSAGCVFRNPNCGHAGALIEQAGLKET
EEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCCC
KIGGAAVSSVHANFIINSGLATSQDVLALIRLIQETVKAKTGAELESEIRYVPYDVNQG
CCCCHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA