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The map label for this gene is lysS [H]

Identifier: 46447164

GI number: 46447164

Start: 1834013

End: 1834915

Strand: Direct

Name: lysS [H]

Synonym: pc1530

Alternate gene names: 46447164

Gene position: 1834013-1834915 (Clockwise)

Preceding gene: 46447163

Following gene: 46447166

Centisome position: 75.96

GC content: 38.54

Gene sequence:

>903_bases
ATGCTCGGTCAATCCCGTCAATTTTTTGCAGCCCGCCAAATTTTGGAAGTTGATTGCCCCTGTCTCAGTTTAAGAGCATC
TATCGATGCCCATATTGACTTAATTCCCGCACTTTATCATCAACAAGAAACCTGCTATCTCCATTCTTCTCCCGAATATG
GAATGAAACGTCTTTTGGCTGAGGGCATGGGTGATTGTTATCAGCTTTCTCACGTTTTTCGCGATGGAGAATTAAGTTCT
AAGCATAATCCTGAATTCATGATGGCTGAATGGTATCGTTTAGGATTTGATTTAGAACAAATGATTGAAGAAACAGTTGC
TTTTATTCGTTTGTTCTTAGGTAACCTCTCCTATCAAACCATTAGTTATCGAGATCTTTTTCTTAAATATACAGGGATTG
ATTACTGTTTAGCAACGAATCAAGAATTATTTGCTTATATTCAAGCCCATCAAATTCCATTTTATACTTCCATTCTTGAA
GAAGGACGGGATGCCCTCCTTAATCTTATTTTAGGAGCTCAAATTGAGTCGCAATTAGGACAACAAGAATTGTGTGTGTT
AGCTTATTATCCAGCCACACAAGCTGCTCTTGCTTGTAAACGCAAGCATGGCGCAGAAGAGGTTGCAGAAAGATTTGAGA
TTTATTACAAAGGAGTTGAATTAGCAAATGGTTACCACGAACTTACAGATGCCAAAGAACAAGAACAAAGATTTTTAGCC
AGCAACAAACAAAGAATCGATCTTGGCAAAAAATCACTCCCTTTAGACTTTTTATTTTTAGAAGCTTTAGAAAAACTTCC
TGATTGTTGTGGCGTTGCTGTTGGATTTGATCGCTTGATGATGTTGCGACACCAATCGACAACTATAGCAGACGTCATTG
CTTGGGGATGGGTGGAAGCATAA

Upstream 100 bases:

>100_bases
AAAAATTAAAGTAGACACTCGTACTGGAGAATATGTTTCGCGAGTTTCGAGCTAATCAGTGCAACTGTCTAAAATTTCAA
GATTACATGATCGAGCTACC

Downstream 100 bases:

>100_bases
AATCCAAAACTAATGGCAACAATAAAGAGCTTTCATTAAATTTATTTTGAAAAGAATTAAAGATTCCATCCCAATGTAAG
AGTATTGCCTAATAACCCCG

Product: putative lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLGQSRQFFAARQILEVDCPCLSLRASIDAHIDLIPALYHQQETCYLHSSPEYGMKRLLAEGMGDCYQLSHVFRDGELSS
KHNPEFMMAEWYRLGFDLEQMIEETVAFIRLFLGNLSYQTISYRDLFLKYTGIDYCLATNQELFAYIQAHQIPFYTSILE
EGRDALLNLILGAQIESQLGQQELCVLAYYPATQAALACKRKHGAEEVAERFEIYYKGVELANGYHELTDAKEQEQRFLA
SNKQRIDLGKKSLPLDFLFLEALEKLPDCCGVAVGFDRLMMLRHQSTTIADVIAWGWVEA

Sequences:

>Translated_300_residues
MLGQSRQFFAARQILEVDCPCLSLRASIDAHIDLIPALYHQQETCYLHSSPEYGMKRLLAEGMGDCYQLSHVFRDGELSS
KHNPEFMMAEWYRLGFDLEQMIEETVAFIRLFLGNLSYQTISYRDLFLKYTGIDYCLATNQELFAYIQAHQIPFYTSILE
EGRDALLNLILGAQIESQLGQQELCVLAYYPATQAALACKRKHGAEEVAERFEIYYKGVELANGYHELTDAKEQEQRFLA
SNKQRIDLGKKSLPLDFLFLEALEKLPDCCGVAVGFDRLMMLRHQSTTIADVIAWGWVEA
>Mature_300_residues
MLGQSRQFFAARQILEVDCPCLSLRASIDAHIDLIPALYHQQETCYLHSSPEYGMKRLLAEGMGDCYQLSHVFRDGELSS
KHNPEFMMAEWYRLGFDLEQMIEETVAFIRLFLGNLSYQTISYRDLFLKYTGIDYCLATNQELFAYIQAHQIPFYTSILE
EGRDALLNLILGAQIESQLGQQELCVLAYYPATQAALACKRKHGAEEVAERFEIYYKGVELANGYHELTDAKEQEQRFLA
SNKQRIDLGKKSLPLDFLFLEALEKLPDCCGVAVGFDRLMMLRHQSTTIADVIAWGWVEA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87082379, Length=302, Percent_Identity=39.4039735099338, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789256, Length=316, Percent_Identity=29.4303797468354, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790571, Length=319, Percent_Identity=28.2131661442006, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 34357; Mature: 34357

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGQSRQFFAARQILEVDCPCLSLRASIDAHIDLIPALYHQQETCYLHSSPEYGMKRLLA
CCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
EGMGDCYQLSHVFRDGELSSKHNPEFMMAEWYRLGFDLEQMIEETVAFIRLFLGNLSYQT
HCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEE
ISYRDLFLKYTGIDYCLATNQELFAYIQAHQIPFYTSILEEGRDALLNLILGAQIESQLG
EHHHHHHHEECCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QQELCVLAYYPATQAALACKRKHGAEEVAERFEIYYKGVELANGYHELTDAKEQEQRFLA
CCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHH
SNKQRIDLGKKSLPLDFLFLEALEKLPDCCGVAVGFDRLMMLRHQSTTIADVIAWGWVEA
CCCCEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLGQSRQFFAARQILEVDCPCLSLRASIDAHIDLIPALYHQQETCYLHSSPEYGMKRLLA
CCCCCHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
EGMGDCYQLSHVFRDGELSSKHNPEFMMAEWYRLGFDLEQMIEETVAFIRLFLGNLSYQT
HCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEE
ISYRDLFLKYTGIDYCLATNQELFAYIQAHQIPFYTSILEEGRDALLNLILGAQIESQLG
EHHHHHHHEECCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QQELCVLAYYPATQAALACKRKHGAEEVAERFEIYYKGVELANGYHELTDAKEQEQRFLA
CCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHH
SNKQRIDLGKKSLPLDFLFLEALEKLPDCCGVAVGFDRLMMLRHQSTTIADVIAWGWVEA
CCCCEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA