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The map label for this gene is grpE

Identifier: 46447132

GI number: 46447132

Start: 1785572

End: 1786207

Strand: Direct

Name: grpE

Synonym: pc1498

Alternate gene names: 46447132

Gene position: 1785572-1786207 (Clockwise)

Preceding gene: 46447131

Following gene: 46447133

Centisome position: 73.95

GC content: 35.69

Gene sequence:

>636_bases
ATGGTTGACAAAGACGAAGAGCAAATAAAACAAAACGTAGAGGAAGATTTATCTTCGACAGTAGAACAAACAGGGGAAGA
AAATATAGAATTCCCATCTGCCCCTAATCATCCCAAACAAGTTCTTGTTACAGATGAAGAATTAAAAGCTTTAAAAAAAG
AAGCGACAGAATACAAAGATAAGTATTTACGACTTTTGGCCGATTCTGAAAATGCACGGAAACGATTACAAAAAGAGCGG
CAGGAAATTAGTCGTTATGCCTTGGAAAATATGGTGGTTGATTTTTTAAAACCTTTAGACAATTTAGAAAATGCGTTAAA
ATTTGCGCAGGGCATGTCTGATGAAGTTAAAAATTGGGCTTTTGGGTTTCAAATGATTTTAACTCAATTTAAAGATGTTT
TAGCAAGTAATGGTATTACAGCTTTAGAATCACAAGGAACTTTCTTTGACCCTCATTTACACGAGGCTATTGAAATGGTT
GAAACGGATTCATATGCTCCTGGCATTATCGTAGAAGAAAATGTTCGCGGATATAAAATGGGGGATCGAATGATTCGCCC
TGCACGTGTCAAAGTTGCAAAAGCAATCTCTGCAATTGATCCGCAAGATAAATCAGAGCTTAATGAAAACAATTAA

Upstream 100 bases:

>100_bases
AAAAAAGAAGAAAGTTTACTGATTGGTCAATCACGTTTCCTCATCGAAGATAAGCGACCATGAAGTTATATAGACCAGAA
TGATAAAGGAGAAAAAAGAA

Downstream 100 bases:

>100_bases
ATAATTAGAGGAGATTTGACTATGAGCCAAAGTCAAACAAAAAAAGGGCGTATAATTGGAATTGACTTAGGAACCACTAA
TTCTTGTGTTGCTGTGATGG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MVDKDEEQIKQNVEEDLSSTVEQTGEENIEFPSAPNHPKQVLVTDEELKALKKEATEYKDKYLRLLADSENARKRLQKER
QEISRYALENMVVDFLKPLDNLENALKFAQGMSDEVKNWAFGFQMILTQFKDVLASNGITALESQGTFFDPHLHEAIEMV
ETDSYAPGIIVEENVRGYKMGDRMIRPARVKVAKAISAIDPQDKSELNENN

Sequences:

>Translated_211_residues
MVDKDEEQIKQNVEEDLSSTVEQTGEENIEFPSAPNHPKQVLVTDEELKALKKEATEYKDKYLRLLADSENARKRLQKER
QEISRYALENMVVDFLKPLDNLENALKFAQGMSDEVKNWAFGFQMILTQFKDVLASNGITALESQGTFFDPHLHEAIEMV
ETDSYAPGIIVEENVRGYKMGDRMIRPARVKVAKAISAIDPQDKSELNENN
>Mature_211_residues
MVDKDEEQIKQNVEEDLSSTVEQTGEENIEFPSAPNHPKQVLVTDEELKALKKEATEYKDKYLRLLADSENARKRLQKER
QEISRYALENMVVDFLKPLDNLENALKFAQGMSDEVKNWAFGFQMILTQFKDVLASNGITALESQGTFFDPHLHEAIEMV
ETDSYAPGIIVEENVRGYKMGDRMIRPARVKVAKAISAIDPQDKSELNENN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=189, Percent_Identity=32.8042328042328, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1788967, Length=145, Percent_Identity=32.4137931034483, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17552458, Length=149, Percent_Identity=30.2013422818792, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=155, Percent_Identity=32.258064516129, Blast_Score=82, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24653432, Length=155, Percent_Identity=34.8387096774194, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PARUW (Q6MB27)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008497.1
- ProteinModelPortal:   Q6MB27
- SMR:   Q6MB27
- STRING:   Q6MB27
- GeneID:   2780420
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1498
- NMPDR:   fig|264201.1.peg.1498
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   Q6MB27
- ProtClustDB:   CLSK871354
- BioCyc:   CPRO264201:PC1498-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24094; Mature: 24094

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKDEEQIKQNVEEDLSSTVEQTGEENIEFPSAPNHPKQVLVTDEELKALKKEATEYKD
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHH
KYLRLLADSENARKRLQKERQEISRYALENMVVDFLKPLDNLENALKFAQGMSDEVKNWA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FGFQMILTQFKDVLASNGITALESQGTFFDPHLHEAIEMVETDSYAPGIIVEENVRGYKM
HHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCHH
GDRMIRPARVKVAKAISAIDPQDKSELNENN
HHHHHCHHHHHHHHHHHHCCCCCHHHCCCCC
>Mature Secondary Structure
MVDKDEEQIKQNVEEDLSSTVEQTGEENIEFPSAPNHPKQVLVTDEELKALKKEATEYKD
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHH
KYLRLLADSENARKRLQKERQEISRYALENMVVDFLKPLDNLENALKFAQGMSDEVKNWA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FGFQMILTQFKDVLASNGITALESQGTFFDPHLHEAIEMVETDSYAPGIIVEENVRGYKM
HHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCHH
GDRMIRPARVKVAKAISAIDPQDKSELNENN
HHHHHCHHHHHHHHHHHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA