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The map label for this gene is ipgD [H]

Identifier: 46446890

GI number: 46446890

Start: 1497846

End: 1499663

Strand: Direct

Name: ipgD [H]

Synonym: pc1256

Alternate gene names: 46446890

Gene position: 1497846-1499663 (Clockwise)

Preceding gene: 46446889

Following gene: 46446891

Centisome position: 62.04

GC content: 36.74

Gene sequence:

>1818_bases
ATGGGGCATGCCTATCAACTATTAAAACAGTTAGAAAATCAAAATGGTCATAATCCGCAAGTAGCTGAAATTTATGAAAA
AGCTTCTACTGAATTATTTCAAGTTCATGAAAATCTTGCTAACGCTTTAAATCCTGCAAACGGAAACACCTTATCGAAAA
AAGAGATCAAAGATTTAAATAAAGCCATCAAAACATCTGAAAAACAAATCGCTCAAATTATTCAAAAAGCTCACATCGAA
GCTGTTGTTCAATTAGGTGGCGAAAAATTTAGATCTGAATTAACAGCCCTTTCAAAAGGAGGGTTTGAAGCCAAATTCAT
TCGTTTTTTTGCCTTGACTTCTTCAACGAAAAATGCGGCTGTCGAGGCTTTTAAACAAGCGACAAGTGCGATGGATCAAG
CAAGTCGAACAATGCCAACAGAGCTTCAAGATATAAAAACAACAGATTTATCCTATGTTCAAAAAGATCAGCTAGAACAA
ATGAAAGCTTTTACAGATGGGATAAGCGACGTTAAGCGTCGAGCAGGTGTGGTTGGACTCAATGGAATTTATCGAGTGAA
ATTGAATTTTTCAGAGGAAAAGATCCAAAAACAAGTCAAATCTTTCTCAGCTCAAAATATAAGCCAACAAGTCAGAACGA
CAATGACTCGTACTGTTTCTTTTGAAAAAGAAGGGCACAGTGTTCCATTTACTAGTACCCAAACACCACTGAATGCAGAA
TTTGATAAGGCTTTAGAAACGAATGGCTCAGCGCGCGTGTTCGAAAAAATTTTTGGTAAAACTGGGGGGATCTCAAGTGC
GAATCGACAAGAAGCGCATTTGATTAATGGATGGGAATCTAACTTAAAAAATTCGGAGGGCCAAATCGTTTATCAGGCAT
TGAGACACGCTATTACTTCTGACAAATATGAAAGCAATGCAACAATTAGAAAAGAAAACTCCAAGCAAGCAGCTGGCGAA
CTCTTAAAAGCCGCTGTGTTACAACATTTATCAGATGTAGGAATGTCTTTAGAAGATGCCCAAGCACAGGGAATTAATCT
TAATTTCAATTCGGTTAGTCTTGTCACACCAGATGATATACGTGCCATTGGTTCAAAAGGCGCTAATGAGAAAAACATGT
TGAATGATCAAATTGCTGCTTTACAAAGTTATGCGGGTCAAAATAAATCTATTGATATCGATGGGTATGCAATTGGTGTT
AACTTGAACATCAATACATTTAATTTTGGAGTGAACGCAGGAGCTGTTAAAATAGGTTTTGGAACAATTAACCAATCCTT
AGAAAATAAAAAAGCTTTTCAAGGTTTAAAAGCCCAAGCAGAAGCTTTGATCAACAATGAAAAAATTGGATTGCCCGAAG
AACGAGCTAAATTACAGAGTCTTTTAGATGACATCACTTTTCTCATGGAATCCCCCAAAGCTTATTTAGATGGAGATAAT
CAATATGAGATTGGCGCAAAAATTATCAATCTCAGTAATGGTATGGATAACATTCAAAAAGGAACTAAATGTGCTTTTAA
CTGTATGAGTGGAAAAGACCGCACAGGAATGATGGATGGTGTTGCAAAAGCCTTTGCCATTATGAATGAAATCAATGGAA
AATTTCCGTCTCACGAAGAGCTAAAATCTGATCCAGAAGTCAGAAAACAATTTCGAGAGATTTTTGTTCCTATCATGATG
GAGATGGGAGGCTTAGATATTACTCGAATCAATACAGGAGCCACTGGTTACAAAGTAGGAAAAGAAGCGAAATTGGCAGA
CTTGCCTGAAGATCAATTTCTGGAAATGATGGGTCTTTCTAAAACCACTTCTAGTTAA

Upstream 100 bases:

>100_bases
TATGAATCTAAATACTGAATTTAATAAAAGTATTCATTCTGTTAGTTTTAATCCATCAACGCAAGATATTAATCATGCTA
GTAGCAGAATTAATGTTCAT

Downstream 100 bases:

>100_bases
CCAAAAGTTTCGGATTTGAAAATTTTTATTTTTCAAAACCGAATCTTTCAAAAACAACCACTTAGAAAGCATTTTTTACT
TTTTATAAAAACGCACTTGA

Product: virulence protein IpgD

Products: NA

Alternate protein names: Effector protein ipgD; Phosphatidylinositol-4,5-bisphosphate 4-phosphatase [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MGHAYQLLKQLENQNGHNPQVAEIYEKASTELFQVHENLANALNPANGNTLSKKEIKDLNKAIKTSEKQIAQIIQKAHIE
AVVQLGGEKFRSELTALSKGGFEAKFIRFFALTSSTKNAAVEAFKQATSAMDQASRTMPTELQDIKTTDLSYVQKDQLEQ
MKAFTDGISDVKRRAGVVGLNGIYRVKLNFSEEKIQKQVKSFSAQNISQQVRTTMTRTVSFEKEGHSVPFTSTQTPLNAE
FDKALETNGSARVFEKIFGKTGGISSANRQEAHLINGWESNLKNSEGQIVYQALRHAITSDKYESNATIRKENSKQAAGE
LLKAAVLQHLSDVGMSLEDAQAQGINLNFNSVSLVTPDDIRAIGSKGANEKNMLNDQIAALQSYAGQNKSIDIDGYAIGV
NLNINTFNFGVNAGAVKIGFGTINQSLENKKAFQGLKAQAEALINNEKIGLPEERAKLQSLLDDITFLMESPKAYLDGDN
QYEIGAKIINLSNGMDNIQKGTKCAFNCMSGKDRTGMMDGVAKAFAIMNEINGKFPSHEELKSDPEVRKQFREIFVPIMM
EMGGLDITRINTGATGYKVGKEAKLADLPEDQFLEMMGLSKTTSS

Sequences:

>Translated_605_residues
MGHAYQLLKQLENQNGHNPQVAEIYEKASTELFQVHENLANALNPANGNTLSKKEIKDLNKAIKTSEKQIAQIIQKAHIE
AVVQLGGEKFRSELTALSKGGFEAKFIRFFALTSSTKNAAVEAFKQATSAMDQASRTMPTELQDIKTTDLSYVQKDQLEQ
MKAFTDGISDVKRRAGVVGLNGIYRVKLNFSEEKIQKQVKSFSAQNISQQVRTTMTRTVSFEKEGHSVPFTSTQTPLNAE
FDKALETNGSARVFEKIFGKTGGISSANRQEAHLINGWESNLKNSEGQIVYQALRHAITSDKYESNATIRKENSKQAAGE
LLKAAVLQHLSDVGMSLEDAQAQGINLNFNSVSLVTPDDIRAIGSKGANEKNMLNDQIAALQSYAGQNKSIDIDGYAIGV
NLNINTFNFGVNAGAVKIGFGTINQSLENKKAFQGLKAQAEALINNEKIGLPEERAKLQSLLDDITFLMESPKAYLDGDN
QYEIGAKIINLSNGMDNIQKGTKCAFNCMSGKDRTGMMDGVAKAFAIMNEINGKFPSHEELKSDPEVRKQFREIFVPIMM
EMGGLDITRINTGATGYKVGKEAKLADLPEDQFLEMMGLSKTTSS
>Mature_604_residues
GHAYQLLKQLENQNGHNPQVAEIYEKASTELFQVHENLANALNPANGNTLSKKEIKDLNKAIKTSEKQIAQIIQKAHIEA
VVQLGGEKFRSELTALSKGGFEAKFIRFFALTSSTKNAAVEAFKQATSAMDQASRTMPTELQDIKTTDLSYVQKDQLEQM
KAFTDGISDVKRRAGVVGLNGIYRVKLNFSEEKIQKQVKSFSAQNISQQVRTTMTRTVSFEKEGHSVPFTSTQTPLNAEF
DKALETNGSARVFEKIFGKTGGISSANRQEAHLINGWESNLKNSEGQIVYQALRHAITSDKYESNATIRKENSKQAAGEL
LKAAVLQHLSDVGMSLEDAQAQGINLNFNSVSLVTPDDIRAIGSKGANEKNMLNDQIAALQSYAGQNKSIDIDGYAIGVN
LNINTFNFGVNAGAVKIGFGTINQSLENKKAFQGLKAQAEALINNEKIGLPEERAKLQSLLDDITFLMESPKAYLDGDNQ
YEIGAKIINLSNGMDNIQKGTKCAFNCMSGKDRTGMMDGVAKAFAIMNEINGKFPSHEELKSDPEVRKQFREIFVPIMME
MGGLDITRINTGATGYKVGKEAKLADLPEDQFLEMMGLSKTTSS

Specific function: Converts phosphatidylinositol-4,5-biphosphate (PtdIns 4,5-P2) to PtdIns 5-P. IpgD is injected by Shigella into the host cell and is required for invasion. The accumulation of PtdIns 5-P causes membrane ruffling and actin cytoskeleton rearrangements at the

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the Mxi-Spa type III secretion system. It is stored in the bacterial cytoplasm associated with the chaperone IpgE before being secreted in response to activation of the type III secretion system [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphatase ipgD/sopB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008108 [H]

Pfam domain/function: PF05925 IpgD [H]

EC number: =3.1.3.78 [H]

Molecular weight: Translated: 66385; Mature: 66254

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHAYQLLKQLENQNGHNPQVAEIYEKASTELFQVHENLANALNPANGNTLSKKEIKDLN
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KAIKTSEKQIAQIIQKAHIEAVVQLGGEKFRSELTALSKGGFEAKFIRFFALTSSTKNAA
HHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHH
VEAFKQATSAMDQASRTMPTELQDIKTTDLSYVQKDQLEQMKAFTDGISDVKRRAGVVGL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
NGIYRVKLNFSEEKIQKQVKSFSAQNISQQVRTTMTRTVSFEKEGHSVPFTSTQTPLNAE
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCH
FDKALETNGSARVFEKIFGKTGGISSANRQEAHLINGWESNLKNSEGQIVYQALRHAITS
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHC
DKYESNATIRKENSKQAAGELLKAAVLQHLSDVGMSLEDAQAQGINLNFNSVSLVTPDDI
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEECCCCEEEECCHHH
RAIGSKGANEKNMLNDQIAALQSYAGQNKSIDIDGYAIGVNLNINTFNFGVNAGAVKIGF
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEEEEEEEEECCCCCEEEEEH
GTINQSLENKKAFQGLKAQAEALINNEKIGLPEERAKLQSLLDDITFLMESPKAYLDGDN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCC
QYEIGAKIINLSNGMDNIQKGTKCAFNCMSGKDRTGMMDGVAKAFAIMNEINGKFPSHEE
CEEECEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
LKSDPEVRKQFREIFVPIMMEMGGLDITRINTGATGYKVGKEAKLADLPEDQFLEMMGLS
HCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
KTTSS
CCCCC
>Mature Secondary Structure 
GHAYQLLKQLENQNGHNPQVAEIYEKASTELFQVHENLANALNPANGNTLSKKEIKDLN
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KAIKTSEKQIAQIIQKAHIEAVVQLGGEKFRSELTALSKGGFEAKFIRFFALTSSTKNAA
HHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHH
VEAFKQATSAMDQASRTMPTELQDIKTTDLSYVQKDQLEQMKAFTDGISDVKRRAGVVGL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
NGIYRVKLNFSEEKIQKQVKSFSAQNISQQVRTTMTRTVSFEKEGHSVPFTSTQTPLNAE
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCH
FDKALETNGSARVFEKIFGKTGGISSANRQEAHLINGWESNLKNSEGQIVYQALRHAITS
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHC
DKYESNATIRKENSKQAAGELLKAAVLQHLSDVGMSLEDAQAQGINLNFNSVSLVTPDDI
CCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEECCCCEEEECCHHH
RAIGSKGANEKNMLNDQIAALQSYAGQNKSIDIDGYAIGVNLNINTFNFGVNAGAVKIGF
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEEEEEEEEECCCCCEEEEEH
GTINQSLENKKAFQGLKAQAEALINNEKIGLPEERAKLQSLLDDITFLMESPKAYLDGDN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCC
QYEIGAKIINLSNGMDNIQKGTKCAFNCMSGKDRTGMMDGVAKAFAIMNEINGKFPSHEE
CEEECEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
LKSDPEVRKQFREIFVPIMMEMGGLDITRINTGATGYKVGKEAKLADLPEDQFLEMMGLS
HCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
KTTSS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8478058; 11115111; 11292750; 12384590; 14573649 [H]