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The map label for this gene is glgX [H]

Identifier: 46446740

GI number: 46446740

Start: 1325660

End: 1327672

Strand: Direct

Name: glgX [H]

Synonym: pc1106

Alternate gene names: 46446740

Gene position: 1325660-1327672 (Clockwise)

Preceding gene: 46446732

Following gene: 46446743

Centisome position: 54.9

GC content: 36.56

Gene sequence:

>2013_bases
ATGAATATACAAATAACACCTGGATCCCCCTTTCCTTTTGGTGCAAATATACAAGAAGGAAAGGTTAATTTTGCTCTTTA
TGCTAAAAATATTGAGAAAATTTCTCTCTGTTTATTCAACGAAAATGATCCTTTAAATCCTTTTAAAGAAATAGAATTGG
AGCCTTCATTAAATAAAACAGGAAACGTTTGGCATATAGCAATTGAATCGCTTCCTCCTTACACATTGTATGCTTTTAGA
GTACCTGTAGAAAACTCAAATTATTTATTAATAGACCCTTACGCAAAATCTATTTATAGCTCTCCTGATTGGGGAAATGC
GAAACCGTACTCCCCTCTTGGTAGAATTATTCCGTTAACAACTTTTGATTGGGAAGGCATACCATCTCCTAAACTTCCTT
CAAAAGATTTAATTATTTATGAAATGCATATACGGGGGCTAACACAGGATCAGTCGAGTCAAGTCTCCCACCCAGGAACT
TATTTGGGAGTGATTGAGAAAATCCCTTATCTTAAAGAGTTAGGAATAAATGCTGTTGAATTAATGCCTATTTACGAATT
TAACGAATCAGAAGCCTTACAAATTAATCCAAAAACTCAGCAAAAATTAGTGAATTACTTTGGATATTCTACAGTAAATT
TTTTTTCTCCGATGAATAGGTATGCAAGTGAAATCCAAGAAAACAAAACGTTAGTTGAATTTAAAACAATGGTTAAAGAA
CTTCACCGTCATGGAATTGAAGTCATTTTAGATGTAGTTTATAACCATACATTTGAAGGAAATCAAATGGGACCCATCCA
ATCCTTTCGAGGGTTAGATAAACATGCTTATTATATGATCGATGAACAAGGAAATTATTTAAACTTTTCAGGTTGTGGAA
ATACTTTTAATGCCAATCATCCGATCGTCAAAGAATTTATTATCCAATCGCTTCGCTACTGGGTGACAGAAATGCGAGTG
GATGGATTTCGTTTTGATTTAGCTTCCATCTTATGCCGGTCTGAAAATGGCACGCCACTCAATCCATCCCCACTCATCGA
AGCAATTTCACACGATCCCATTTTATCACAAACCAAATTGATCGCTGAAGCCTGGGACGCAGGTGGCTTATACCAAGTCG
GTGGATTTTATCCCGGCCAACGTTGGGCAGAGTGGAACGGTCATTATCGAGATATCGTGAGGCGTTTTATTAAAGGAACT
TCAGGCCATAAAACAGCTTTTGCGACAGCATTAAGCGGATCACAAGATCTTTATGGTTGGCGAGGAACTCCTTGTTGTAG
CATTAATTTCATCACAGCTCATGATGGATTTAGCCTGGCAGATTTAGTAACATACAATGACAAGCACAATTTAGATAATG
GCGAAGAGAATAGAGACGGATTTGATCACAATGATAGCTGGAACTGTGGAATAGAGGGGCATTCTAATAATAAAAAAATT
GTCGCTTTACGAGAAAGACAAATTCGTAATTTCTTATTAGCCTTATTGGTTTCGCAAGGAATCCCCATGATATTAATGGG
TGATGAATACGCGCATACAAGAGATGGAAATAATAACACCTGGTGTCAAGACAATAAATTGAATTGGTTTTTATGGGATA
AACTTCTGGAGAAACAAAGTGTTTTCCGATTTTTTAAATCATTAATCACGTTTAGAAAAAATTATCCTCTCCTTAAACGT
GATACATTTTTAGAAGAAACGGATGTCACTTGGCATGGGCAAGTGCCTTTCAACCCCGATTGGGAAAATGACAATAAATT
CATTGCTTTTTCACTTAATATTCCACATGAAGGCCCTGATCTCTATGTTGCTTTCAATGCCTCTCACGTTGTTTTAACAG
TTACTATTCCTCCTGCAAGGCAAGGAATGCATTGGGTTTGGTTAGTCAATACCCATAACGTGGCTCCAGGAGATTTTTTT
GAAGAAAGTTGTCGTAAACGTGTCAATTCAAACACACTTAAAATTCCTTCATACACTTCTATTGCGCTAAAAGCAGAAGT
AGATAGGTCGTAA

Upstream 100 bases:

>100_bases
AGCTTAAATTCATCATCTTTAATCTGTTGGAATAAATTTTTTGATCCTGTATGAAAAGGATAGTCAATAAATATTTTAAT
TCTTTCCCCCCAAAACTCAG

Downstream 100 bases:

>100_bases
ACGTGTCAATTCAAACACACTTAAAATTCCTTCATATACTTCTATTGCGCTAAAAGAAGAAGTAGATAGATAGAAAGGAA
AGTCGATGCTGGTTACCAAA

Product: isoamylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MNIQITPGSPFPFGANIQEGKVNFALYAKNIEKISLCLFNENDPLNPFKEIELEPSLNKTGNVWHIAIESLPPYTLYAFR
VPVENSNYLLIDPYAKSIYSSPDWGNAKPYSPLGRIIPLTTFDWEGIPSPKLPSKDLIIYEMHIRGLTQDQSSQVSHPGT
YLGVIEKIPYLKELGINAVELMPIYEFNESEALQINPKTQQKLVNYFGYSTVNFFSPMNRYASEIQENKTLVEFKTMVKE
LHRHGIEVILDVVYNHTFEGNQMGPIQSFRGLDKHAYYMIDEQGNYLNFSGCGNTFNANHPIVKEFIIQSLRYWVTEMRV
DGFRFDLASILCRSENGTPLNPSPLIEAISHDPILSQTKLIAEAWDAGGLYQVGGFYPGQRWAEWNGHYRDIVRRFIKGT
SGHKTAFATALSGSQDLYGWRGTPCCSINFITAHDGFSLADLVTYNDKHNLDNGEENRDGFDHNDSWNCGIEGHSNNKKI
VALRERQIRNFLLALLVSQGIPMILMGDEYAHTRDGNNNTWCQDNKLNWFLWDKLLEKQSVFRFFKSLITFRKNYPLLKR
DTFLEETDVTWHGQVPFNPDWENDNKFIAFSLNIPHEGPDLYVAFNASHVVLTVTIPPARQGMHWVWLVNTHNVAPGDFF
EESCRKRVNSNTLKIPSYTSIALKAEVDRS

Sequences:

>Translated_670_residues
MNIQITPGSPFPFGANIQEGKVNFALYAKNIEKISLCLFNENDPLNPFKEIELEPSLNKTGNVWHIAIESLPPYTLYAFR
VPVENSNYLLIDPYAKSIYSSPDWGNAKPYSPLGRIIPLTTFDWEGIPSPKLPSKDLIIYEMHIRGLTQDQSSQVSHPGT
YLGVIEKIPYLKELGINAVELMPIYEFNESEALQINPKTQQKLVNYFGYSTVNFFSPMNRYASEIQENKTLVEFKTMVKE
LHRHGIEVILDVVYNHTFEGNQMGPIQSFRGLDKHAYYMIDEQGNYLNFSGCGNTFNANHPIVKEFIIQSLRYWVTEMRV
DGFRFDLASILCRSENGTPLNPSPLIEAISHDPILSQTKLIAEAWDAGGLYQVGGFYPGQRWAEWNGHYRDIVRRFIKGT
SGHKTAFATALSGSQDLYGWRGTPCCSINFITAHDGFSLADLVTYNDKHNLDNGEENRDGFDHNDSWNCGIEGHSNNKKI
VALRERQIRNFLLALLVSQGIPMILMGDEYAHTRDGNNNTWCQDNKLNWFLWDKLLEKQSVFRFFKSLITFRKNYPLLKR
DTFLEETDVTWHGQVPFNPDWENDNKFIAFSLNIPHEGPDLYVAFNASHVVLTVTIPPARQGMHWVWLVNTHNVAPGDFF
EESCRKRVNSNTLKIPSYTSIALKAEVDRS
>Mature_670_residues
MNIQITPGSPFPFGANIQEGKVNFALYAKNIEKISLCLFNENDPLNPFKEIELEPSLNKTGNVWHIAIESLPPYTLYAFR
VPVENSNYLLIDPYAKSIYSSPDWGNAKPYSPLGRIIPLTTFDWEGIPSPKLPSKDLIIYEMHIRGLTQDQSSQVSHPGT
YLGVIEKIPYLKELGINAVELMPIYEFNESEALQINPKTQQKLVNYFGYSTVNFFSPMNRYASEIQENKTLVEFKTMVKE
LHRHGIEVILDVVYNHTFEGNQMGPIQSFRGLDKHAYYMIDEQGNYLNFSGCGNTFNANHPIVKEFIIQSLRYWVTEMRV
DGFRFDLASILCRSENGTPLNPSPLIEAISHDPILSQTKLIAEAWDAGGLYQVGGFYPGQRWAEWNGHYRDIVRRFIKGT
SGHKTAFATALSGSQDLYGWRGTPCCSINFITAHDGFSLADLVTYNDKHNLDNGEENRDGFDHNDSWNCGIEGHSNNKKI
VALRERQIRNFLLALLVSQGIPMILMGDEYAHTRDGNNNTWCQDNKLNWFLWDKLLEKQSVFRFFKSLITFRKNYPLLKR
DTFLEETDVTWHGQVPFNPDWENDNKFIAFSLNIPHEGPDLYVAFNASHVVLTVTIPPARQGMHWVWLVNTHNVAPGDFF
EESCRKRVNSNTLKIPSYTSIALKAEVDRS

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=209, Percent_Identity=28.2296650717703, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI2367229, Length=646, Percent_Identity=38.390092879257, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6320826, Length=214, Percent_Identity=29.4392523364486, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28573410, Length=210, Percent_Identity=29.0476190476191, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 76625; Mature: 76625

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQITPGSPFPFGANIQEGKVNFALYAKNIEKISLCLFNENDPLNPFKEIELEPSLNKT
CEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHEEECCCCCCC
GNVWHIAIESLPPYTLYAFRVPVENSNYLLIDPYAKSIYSSPDWGNAKPYSPLGRIIPLT
CCEEEEEECCCCCEEEEEEEEEECCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCEEEEE
TFDWEGIPSPKLPSKDLIIYEMHIRGLTQDQSSQVSHPGTYLGVIEKIPYLKELGINAVE
EECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCEEE
LMPIYEFNESEALQINPKTQQKLVNYFGYSTVNFFSPMNRYASEIQENKTLVEFKTMVKE
EEEEEECCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LHRHGIEVILDVVYNHTFEGNQMGPIQSFRGLDKHAYYMIDEQGNYLNFSGCGNTFNANH
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCCCC
PIVKEFIIQSLRYWVTEMRVDGFRFDLASILCRSENGTPLNPSPLIEAISHDPILSQTKL
CHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHEECCCCCCCCHHHHHHHHCCCCCHHHHHH
IAEAWDAGGLYQVGGFYPGQRWAEWNGHYRDIVRRFIKGTSGHKTAFATALSGSQDLYGW
HHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCC
RGTPCCSINFITAHDGFSLADLVTYNDKHNLDNGEENRDGFDHNDSWNCGIEGHSNNKKI
CCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEE
VALRERQIRNFLLALLVSQGIPMILMGDEYAHTRDGNNNTWCQDNKLNWFLWDKLLEKQS
EEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEECCCCEEHHHHHHHHHHH
VFRFFKSLITFRKNYPLLKRDTFLEETDVTWHGQVPFNPDWENDNKFIAFSLNIPHEGPD
HHHHHHHHHHHHHCCCCEECCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEEECCCCCCC
LYVAFNASHVVLTVTIPPARQGMHWVWLVNTHNVAPGDFFEESCRKRVNSNTLKIPSYTS
EEEEEECCEEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCCE
IALKAEVDRS
EEEEEECCCC
>Mature Secondary Structure
MNIQITPGSPFPFGANIQEGKVNFALYAKNIEKISLCLFNENDPLNPFKEIELEPSLNKT
CEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHEEECCCCCCC
GNVWHIAIESLPPYTLYAFRVPVENSNYLLIDPYAKSIYSSPDWGNAKPYSPLGRIIPLT
CCEEEEEECCCCCEEEEEEEEEECCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCEEEEE
TFDWEGIPSPKLPSKDLIIYEMHIRGLTQDQSSQVSHPGTYLGVIEKIPYLKELGINAVE
EECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCEEE
LMPIYEFNESEALQINPKTQQKLVNYFGYSTVNFFSPMNRYASEIQENKTLVEFKTMVKE
EEEEEECCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LHRHGIEVILDVVYNHTFEGNQMGPIQSFRGLDKHAYYMIDEQGNYLNFSGCGNTFNANH
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCCCC
PIVKEFIIQSLRYWVTEMRVDGFRFDLASILCRSENGTPLNPSPLIEAISHDPILSQTKL
CHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHEECCCCCCCCHHHHHHHHCCCCCHHHHHH
IAEAWDAGGLYQVGGFYPGQRWAEWNGHYRDIVRRFIKGTSGHKTAFATALSGSQDLYGW
HHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCC
RGTPCCSINFITAHDGFSLADLVTYNDKHNLDNGEENRDGFDHNDSWNCGIEGHSNNKKI
CCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEE
VALRERQIRNFLLALLVSQGIPMILMGDEYAHTRDGNNNTWCQDNKLNWFLWDKLLEKQS
EEEHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEECCCCEEHHHHHHHHHHH
VFRFFKSLITFRKNYPLLKRDTFLEETDVTWHGQVPFNPDWENDNKFIAFSLNIPHEGPD
HHHHHHHHHHHHHCCCCEECCCCCCCCCCEEECCCCCCCCCCCCCEEEEEEEECCCCCCC
LYVAFNASHVVLTVTIPPARQGMHWVWLVNTHNVAPGDFFEESCRKRVNSNTLKIPSYTS
EEEEEECCEEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCCCE
IALKAEVDRS
EEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]