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The map label for this gene is vacB [H]

Identifier: 46446682

GI number: 46446682

Start: 1247563

End: 1249890

Strand: Direct

Name: vacB [H]

Synonym: pc1048

Alternate gene names: 46446682

Gene position: 1247563-1249890 (Clockwise)

Preceding gene: 46446680

Following gene: 46446683

Centisome position: 51.67

GC content: 36.08

Gene sequence:

>2328_bases
TTGATTAATTCTATGGTAAAAACCAGCAAAAAGTCAAAACAAAAGGCTCCCACAATGCGGAGTGCAAAAGAAGAAAAACT
ATTCAATAATTTATTAAAAATTACAGAACAGTTTATGCGCGGTAAAAGCTTTATGCCTTTATCCCTAGAAGTTTTGATGC
AACGTCTCGCCCTTCCTCCTCAACACAAGGAAATTTTTCAAGAAGTTCTTAATAAATTACAGCAACAGGGATTAATTGAA
TCGAAAGAAGAGAAATATGAATGGAAAAAATCCCAAGCAGATGTGGTGACGGGAATTTTAAAAATGCATCCCCGAGGATT
TGGTTTCGTTCAAGTTACGATTCCTAAAAATTTTGAACAAGACATTTTTATTCCCAAACATTTAACAAAAAATGGTGTAG
ACGGAGACGTTGTCGAAGTTATCCTTAACCAAGAAGTTATCTCTGATAAGGGACCTGAAGGGAAAATTGTTGCTATTCTT
TCTCGTGGAAGAACTCATATGGCTGGAATCATCCGCCAGCTTGATAAAGATAGGATGTTCATTGCTCATGTTCCGCTACT
TGGTACTCAACAACGAGTTGTCGTTCATCCAACCGAAGAACATCAATTGCAAATTGGTGATCGCATTGTCATGGAAGTCA
TCGATTGGGGGTCAAAAGAAACAGAAACAATCTGTCGCTTTTCTCATTATTTAGGTCACATTTCAGATCCTTCATGTGAT
GTCTATGCAGCAATTGAAGAATTTGAGTTAAGGTCTGATTTTCAAACAGCTGCGATTGAAGAAGCTAATAACCTGGGAAA
GAGTGTTTCTAAAAAAGATATTCAAAATAGAGAAGATTTAAGAGAACTAATTTCTCTGACTATTGACCCAGATACAGCAA
AAGACTTTGATGATGCTCTTAGTTTAACACGTGACGCAGAAGGTATCTATAATTTAGCCGTTCACATTGCCGATGTTTCT
CATTATGTGCAGCCTGGAACTGCTCTCGATGAAGAAGCTAAAGCACGATGTAACTCTACTTATTTTCCAGGATATTGCGT
CCCCATGCTTCCTGGGGCATTATCTGAAAATTTATGTAGCTTAAGACCAAATGTAAACCGGTTAGCTGTTTCTGTTTTAA
TGCGCATTGACAGTTCGGGAAATTTACTTGATTATCGTTTTACTCGCTCTGTGATTAAAAGCGCGAAACGCTTTACCTAT
AAAGAAGCCAAAAAAGTCTTAGATGGGCAAAAAAACAGCCCTTATAAACCGCATTTAGAACTAATGGTTGAATTATGTCG
TTTATTAAAACAAAAACGTTACGAACGCGGTAGTATTGAATTTTCTCTACCGGAACTCGTTATTTTAGTAGATGAAAAAG
GAAAGCCTTATTCAACCGAATATATTACTTATGATATTACACATCAAATGGTTGAAGAATTCATGTTGAAGGCTAATGAA
ATCGTTGCGTGGGATTTAAGCCAAAAAGGGAAAAATTTGACTTATCGTATTCACGATGAACCTTCAGAAGAAAATCTTCG
TGATTTTTCCACGTTAGCTGCCGCTTTTGGATTCAAATTACCTGATCTTCCAACTCCTCAAGATCTTCAAAAATTATTTG
AAAAAGCTGCAGAGACAGCTTACAGTAATTATTTAGCATCGAGCTACATTAGGCGAATGCGTTTAGCTGCTTATTCAGCA
GAAAATATTGGTCATTATGGATTAAGCTTAACCCATTACTGCCATTTTACCAGCCCCATTAGACGCTACGTTGATTTAGT
TGTTCACCGTATTTTATTTGGCGAAAGTGATCGTTTTGATGATCTTCAAGAAATTGCTAATTATTGTTCGGAGCAAGAAA
GAATCAGCGCAAAAGCTGAAAATAGCGTTGTGATATTAAAAAAACTTCGACTCCTAGAAGAAAGACATCAAAAGGCCCCG
GAACATGAGTATCAAGCTATCATTACTCGAGTCAAAAATTTCGGCATTTATTTTGAGATCTTTGATCTTCTTTTGGAAGG
ATTTATTCATATTTCCGATTTGGGTGAAGATTATTTTGTCTATGAAGAAGAAAAAATGCTTTTGAGAGGTGCTAAACACG
GAGCAAGATATTCTCCTGGCGATAATCTCGTTGTTATGTTAGAAAAAATCGATTTTATTTCTCAGGAAACAAAATGGTAT
GTTTTACCATCCGTTTCTACACCTAAGAAATCCATTGCACGTAAAAGATCTGCGTCCAAAGAATATCAAAAACATTTAAA
TAAGAAATGCTCAACCGGCAAATATAATCTCAAAAAAAAACAAGCGGCTTCTATGACTAAACTTGGGAAGAAAAAACGGA
AAAGATAA

Upstream 100 bases:

>100_bases
TTAAAACATTTAGTTTTTATCATGAGGATATGTTTAAGAAAGAGTTTAAAAGCTCAGAATCTATATTTTCTCGCGTTTTT
TAAATCACATCGTAAGGTAT

Downstream 100 bases:

>100_bases
AAAATTAAAAGAGAAGCAAGATGATGAAAGCTCAAACATTTGATAACAAATGTTTTAAACTATTCGATTATCTCATTTTT
GTTTACAATGATTTTATCCA

Product: putative ribonuclease R

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 775; Mature: 775

Protein sequence:

>775_residues
MINSMVKTSKKSKQKAPTMRSAKEEKLFNNLLKITEQFMRGKSFMPLSLEVLMQRLALPPQHKEIFQEVLNKLQQQGLIE
SKEEKYEWKKSQADVVTGILKMHPRGFGFVQVTIPKNFEQDIFIPKHLTKNGVDGDVVEVILNQEVISDKGPEGKIVAIL
SRGRTHMAGIIRQLDKDRMFIAHVPLLGTQQRVVVHPTEEHQLQIGDRIVMEVIDWGSKETETICRFSHYLGHISDPSCD
VYAAIEEFELRSDFQTAAIEEANNLGKSVSKKDIQNREDLRELISLTIDPDTAKDFDDALSLTRDAEGIYNLAVHIADVS
HYVQPGTALDEEAKARCNSTYFPGYCVPMLPGALSENLCSLRPNVNRLAVSVLMRIDSSGNLLDYRFTRSVIKSAKRFTY
KEAKKVLDGQKNSPYKPHLELMVELCRLLKQKRYERGSIEFSLPELVILVDEKGKPYSTEYITYDITHQMVEEFMLKANE
IVAWDLSQKGKNLTYRIHDEPSEENLRDFSTLAAAFGFKLPDLPTPQDLQKLFEKAAETAYSNYLASSYIRRMRLAAYSA
ENIGHYGLSLTHYCHFTSPIRRYVDLVVHRILFGESDRFDDLQEIANYCSEQERISAKAENSVVILKKLRLLEERHQKAP
EHEYQAIITRVKNFGIYFEIFDLLLEGFIHISDLGEDYFVYEEEKMLLRGAKHGARYSPGDNLVVMLEKIDFISQETKWY
VLPSVSTPKKSIARKRSASKEYQKHLNKKCSTGKYNLKKKQAASMTKLGKKKRKR

Sequences:

>Translated_775_residues
MINSMVKTSKKSKQKAPTMRSAKEEKLFNNLLKITEQFMRGKSFMPLSLEVLMQRLALPPQHKEIFQEVLNKLQQQGLIE
SKEEKYEWKKSQADVVTGILKMHPRGFGFVQVTIPKNFEQDIFIPKHLTKNGVDGDVVEVILNQEVISDKGPEGKIVAIL
SRGRTHMAGIIRQLDKDRMFIAHVPLLGTQQRVVVHPTEEHQLQIGDRIVMEVIDWGSKETETICRFSHYLGHISDPSCD
VYAAIEEFELRSDFQTAAIEEANNLGKSVSKKDIQNREDLRELISLTIDPDTAKDFDDALSLTRDAEGIYNLAVHIADVS
HYVQPGTALDEEAKARCNSTYFPGYCVPMLPGALSENLCSLRPNVNRLAVSVLMRIDSSGNLLDYRFTRSVIKSAKRFTY
KEAKKVLDGQKNSPYKPHLELMVELCRLLKQKRYERGSIEFSLPELVILVDEKGKPYSTEYITYDITHQMVEEFMLKANE
IVAWDLSQKGKNLTYRIHDEPSEENLRDFSTLAAAFGFKLPDLPTPQDLQKLFEKAAETAYSNYLASSYIRRMRLAAYSA
ENIGHYGLSLTHYCHFTSPIRRYVDLVVHRILFGESDRFDDLQEIANYCSEQERISAKAENSVVILKKLRLLEERHQKAP
EHEYQAIITRVKNFGIYFEIFDLLLEGFIHISDLGEDYFVYEEEKMLLRGAKHGARYSPGDNLVVMLEKIDFISQETKWY
VLPSVSTPKKSIARKRSASKEYQKHLNKKCSTGKYNLKKKQAASMTKLGKKKRKR
>Mature_775_residues
MINSMVKTSKKSKQKAPTMRSAKEEKLFNNLLKITEQFMRGKSFMPLSLEVLMQRLALPPQHKEIFQEVLNKLQQQGLIE
SKEEKYEWKKSQADVVTGILKMHPRGFGFVQVTIPKNFEQDIFIPKHLTKNGVDGDVVEVILNQEVISDKGPEGKIVAIL
SRGRTHMAGIIRQLDKDRMFIAHVPLLGTQQRVVVHPTEEHQLQIGDRIVMEVIDWGSKETETICRFSHYLGHISDPSCD
VYAAIEEFELRSDFQTAAIEEANNLGKSVSKKDIQNREDLRELISLTIDPDTAKDFDDALSLTRDAEGIYNLAVHIADVS
HYVQPGTALDEEAKARCNSTYFPGYCVPMLPGALSENLCSLRPNVNRLAVSVLMRIDSSGNLLDYRFTRSVIKSAKRFTY
KEAKKVLDGQKNSPYKPHLELMVELCRLLKQKRYERGSIEFSLPELVILVDEKGKPYSTEYITYDITHQMVEEFMLKANE
IVAWDLSQKGKNLTYRIHDEPSEENLRDFSTLAAAFGFKLPDLPTPQDLQKLFEKAAETAYSNYLASSYIRRMRLAAYSA
ENIGHYGLSLTHYCHFTSPIRRYVDLVVHRILFGESDRFDDLQEIANYCSEQERISAKAENSVVILKKLRLLEERHQKAP
EHEYQAIITRVKNFGIYFEIFDLLLEGFIHISDLGEDYFVYEEEKMLLRGAKHGARYSPGDNLVVMLEKIDFISQETKWY
VLPSVSTPKKSIARKRSASKEYQKHLNKKCSTGKYNLKKKQAASMTKLGKKKRKR

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=453, Percent_Identity=30.6843267108168, Blast_Score=199, Evalue=1e-50,
Organism=Homo sapiens, GI190014625, Length=453, Percent_Identity=30.6843267108168, Blast_Score=199, Evalue=1e-50,
Organism=Homo sapiens, GI134288890, Length=522, Percent_Identity=28.3524904214559, Blast_Score=185, Evalue=2e-46,
Organism=Homo sapiens, GI19115966, Length=534, Percent_Identity=28.0898876404494, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI219521928, Length=534, Percent_Identity=28.0898876404494, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI156105695, Length=364, Percent_Identity=27.7472527472527, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI156105693, Length=364, Percent_Identity=27.7472527472527, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI87082383, Length=668, Percent_Identity=29.3413173652695, Blast_Score=295, Evalue=1e-80,
Organism=Escherichia coli, GI1787542, Length=589, Percent_Identity=25.8064516129032, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17553506, Length=437, Percent_Identity=31.5789473684211, Blast_Score=186, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI212645896, Length=339, Percent_Identity=33.3333333333333, Blast_Score=162, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6324552, Length=508, Percent_Identity=29.7244094488189, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320499, Length=386, Percent_Identity=22.279792746114, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323943, Length=427, Percent_Identity=22.9508196721311, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24649634, Length=460, Percent_Identity=32.1739130434783, Blast_Score=206, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24654592, Length=508, Percent_Identity=26.7716535433071, Blast_Score=161, Evalue=2e-39,
Organism=Drosophila melanogaster, GI19922976, Length=508, Percent_Identity=26.7716535433071, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24654597, Length=508, Percent_Identity=26.7716535433071, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805 [H]

Pfam domain/function: PF08206 OB_RNB; PF00773 RNB [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 88987; Mature: 88987

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSMVKTSKKSKQKAPTMRSAKEEKLFNNLLKITEQFMRGKSFMPLSLEVLMQRLALPP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
QHKEIFQEVLNKLQQQGLIESKEEKYEWKKSQADVVTGILKMHPRGFGFVQVTIPKNFEQ
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
DIFIPKHLTKNGVDGDVVEVILNQEVISDKGPEGKIVAILSRGRTHMAGIIRQLDKDRMF
CCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEE
IAHVPLLGTQQRVVVHPTEEHQLQIGDRIVMEVIDWGSKETETICRFSHYLGHISDPSCD
EEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCH
VYAAIEEFELRSDFQTAAIEEANNLGKSVSKKDIQNREDLRELISLTIDPDTAKDFDDAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCHHHHH
SLTRDAEGIYNLAVHIADVSHYVQPGTALDEEAKARCNSTYFPGYCVPMLPGALSENLCS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHCCCHHHHHHHH
LRPNVNRLAVSVLMRIDSSGNLLDYRFTRSVIKSAKRFTYKEAKKVLDGQKNSPYKPHLE
CCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LMVELCRLLKQKRYERGSIEFSLPELVILVDEKGKPYSTEYITYDITHQMVEEFMLKANE
HHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
IVAWDLSQKGKNLTYRIHDEPSEENLRDFSTLAAAFGFKLPDLPTPQDLQKLFEKAAETA
EEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
YSNYLASSYIRRMRLAAYSAENIGHYGLSLTHYCHFTSPIRRYVDLVVHRILFGESDRFD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DLQEIANYCSEQERISAKAENSVVILKKLRLLEERHQKAPEHEYQAIITRVKNFGIYFEI
HHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHH
FDLLLEGFIHISDLGEDYFVYEEEKMLLRGAKHGARYSPGDNLVVMLEKIDFISQETKWY
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHCCCCCEE
VLPSVSTPKKSIARKRSASKEYQKHLNKKCSTGKYNLKKKQAASMTKLGKKKRKR
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINSMVKTSKKSKQKAPTMRSAKEEKLFNNLLKITEQFMRGKSFMPLSLEVLMQRLALPP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
QHKEIFQEVLNKLQQQGLIESKEEKYEWKKSQADVVTGILKMHPRGFGFVQVTIPKNFEQ
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
DIFIPKHLTKNGVDGDVVEVILNQEVISDKGPEGKIVAILSRGRTHMAGIIRQLDKDRMF
CCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEE
IAHVPLLGTQQRVVVHPTEEHQLQIGDRIVMEVIDWGSKETETICRFSHYLGHISDPSCD
EEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCH
VYAAIEEFELRSDFQTAAIEEANNLGKSVSKKDIQNREDLRELISLTIDPDTAKDFDDAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCHHHHH
SLTRDAEGIYNLAVHIADVSHYVQPGTALDEEAKARCNSTYFPGYCVPMLPGALSENLCS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHCCCHHHHHHHH
LRPNVNRLAVSVLMRIDSSGNLLDYRFTRSVIKSAKRFTYKEAKKVLDGQKNSPYKPHLE
CCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LMVELCRLLKQKRYERGSIEFSLPELVILVDEKGKPYSTEYITYDITHQMVEEFMLKANE
HHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
IVAWDLSQKGKNLTYRIHDEPSEENLRDFSTLAAAFGFKLPDLPTPQDLQKLFEKAAETA
EEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
YSNYLASSYIRRMRLAAYSAENIGHYGLSLTHYCHFTSPIRRYVDLVVHRILFGESDRFD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DLQEIANYCSEQERISAKAENSVVILKKLRLLEERHQKAPEHEYQAIITRVKNFGIYFEI
HHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHH
FDLLLEGFIHISDLGEDYFVYEEEKMLLRGAKHGARYSPGDNLVVMLEKIDFISQETKWY
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHCCCCCEE
VLPSVSTPKKSIARKRSASKEYQKHLNKKCSTGKYNLKKKQAASMTKLGKKKRKR
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]