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The map label for this gene is potD [H]

Identifier: 46446508

GI number: 46446508

Start: 1056397

End: 1057434

Strand: Direct

Name: potD [H]

Synonym: pc0874

Alternate gene names: 46446508

Gene position: 1056397-1057434 (Clockwise)

Preceding gene: 46446507

Following gene: 46446511

Centisome position: 43.75

GC content: 34.78

Gene sequence:

>1038_bases
ATGAAAAAATACTTTCTCTTATTACTTTCCCTGTTCGCTAGCCTTAGTATTTTTACGGCATGCCAAAATACGCTTGTTCC
AGAGCTGCACATCATGATTTGGAGTGATTACATCAAACCTGAAATCATAGAAAAATTTCAGGAAAGTCACCATTGTCGTG
TTATTATTGATACCTTTGATTCTAATGAATCGATGTATGCTAAGGTAAAGTTAGGAGCCGGTGGTTATGATATCATTTTT
CCAAGCAACTATTTCATGCAAATCATGAATCAACAAAATATGCTCGAGCCTTTAAATAAAGAATTAATTCCCAATCTTAA
AAATTTAGATCCTGCGTATACTAACAAAGTCGATCGCTCATTGCTCAATTTAGGTGTTCCTTATATGGTTTCCAGTGCTG
GAATCGTTTATCGTCAAGATAAAGTTGAAAATTTAGAAGTTTCTTGGGGGGTATTTGGCAAATCGAATTATAAAGGTCGC
ATGACGATGCTTAACGACGTGCGGGAGACTTTAGGCGCTGCCTTGAAATTTTTAGGCTATAGTGCCAATACAACTCAATT
GCAACAAATCGATCAAGCAGCGCAAGTGCTCATCGATTGGAAAACTAATTTAGCCAAATTTGAAAGCGAACAATATAAAG
CGGGGATTTCTAGTGCTGAGTATCTCATTGTCAATGGTTATAATGGGGATGCATTGCAAGTTATGAAAGAAAACAAAAAT
GTGAATTTTTTCTATCCAAAAGAAGGCATTAGTTTTTCTATGGATTTTATGGTGATTCCTAAAGAAGCTATTCAAAAAGA
ATTGGCTCATGCATTTATTAATTTCCTTTTAGATGGTAAAGTAGCTGCTGAAAACATCGAGCATTCTCTCTTTTTAAGTC
CAAATCTAGCTGCTTATGAGTATTTATCCAGTGAATTAAAAAATAATACTGTTCTATTTATGCCGAAAGAGGTTTTAGAT
AAAGCTGAGTTGATTAAATATCTGGGTGAAAAGGGTATTCTTTACAACAAAGCTTGGGATAAAGTAAAGGCGAGTTAA

Upstream 100 bases:

>100_bases
TTGGATCGACACCTATAGTGAATGCGCTTTCTGTTCTGATTTTGGGCGTGACATTTGTATTTATATTTTTTACACAACGT
TTATCTAAAGAGGATTGAGC

Downstream 100 bases:

>100_bases
TGCTCGCTTTCAAAATCTTTATTGACAGCTGATTCTTACCTGTCGTCAAAAGGTTGTTGTTAGCTCAAGAAAGTTAACGT
TGCGATATAAGCTGGGCTCC

Product: putative spermidine/putrescine-binding protein precursor, component of ABC transporter system

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKKYFLLLLSLFASLSIFTACQNTLVPELHIMIWSDYIKPEIIEKFQESHHCRVIIDTFDSNESMYAKVKLGAGGYDIIF
PSNYFMQIMNQQNMLEPLNKELIPNLKNLDPAYTNKVDRSLLNLGVPYMVSSAGIVYRQDKVENLEVSWGVFGKSNYKGR
MTMLNDVRETLGAALKFLGYSANTTQLQQIDQAAQVLIDWKTNLAKFESEQYKAGISSAEYLIVNGYNGDALQVMKENKN
VNFFYPKEGISFSMDFMVIPKEAIQKELAHAFINFLLDGKVAAENIEHSLFLSPNLAAYEYLSSELKNNTVLFMPKEVLD
KAELIKYLGEKGILYNKAWDKVKAS

Sequences:

>Translated_345_residues
MKKYFLLLLSLFASLSIFTACQNTLVPELHIMIWSDYIKPEIIEKFQESHHCRVIIDTFDSNESMYAKVKLGAGGYDIIF
PSNYFMQIMNQQNMLEPLNKELIPNLKNLDPAYTNKVDRSLLNLGVPYMVSSAGIVYRQDKVENLEVSWGVFGKSNYKGR
MTMLNDVRETLGAALKFLGYSANTTQLQQIDQAAQVLIDWKTNLAKFESEQYKAGISSAEYLIVNGYNGDALQVMKENKN
VNFFYPKEGISFSMDFMVIPKEAIQKELAHAFINFLLDGKVAAENIEHSLFLSPNLAAYEYLSSELKNNTVLFMPKEVLD
KAELIKYLGEKGILYNKAWDKVKAS
>Mature_345_residues
MKKYFLLLLSLFASLSIFTACQNTLVPELHIMIWSDYIKPEIIEKFQESHHCRVIIDTFDSNESMYAKVKLGAGGYDIIF
PSNYFMQIMNQQNMLEPLNKELIPNLKNLDPAYTNKVDRSLLNLGVPYMVSSAGIVYRQDKVENLEVSWGVFGKSNYKGR
MTMLNDVRETLGAALKFLGYSANTTQLQQIDQAAQVLIDWKTNLAKFESEQYKAGISSAEYLIVNGYNGDALQVMKENKN
VNFFYPKEGISFSMDFMVIPKEAIQKELAHAFINFLLDGKVAAENIEHSLFLSPNLAAYEYLSSELKNNTVLFMPKEVLD
KAELIKYLGEKGILYNKAWDKVKAS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=319, Percent_Identity=33.2288401253918, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1787078, Length=353, Percent_Identity=30.3116147308782, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39300; Mature: 39300

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYFLLLLSLFASLSIFTACQNTLVPELHIMIWSDYIKPEIIEKFQESHHCRVIIDTFD
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEC
SNESMYAKVKLGAGGYDIIFPSNYFMQIMNQQNMLEPLNKELIPNLKNLDPAYTNKVDRS
CCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LLNLGVPYMVSSAGIVYRQDKVENLEVSWGVFGKSNYKGRMTMLNDVRETLGAALKFLGY
HHHCCCCHHHHCCCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
SANTTQLQQIDQAAQVLIDWKTNLAKFESEQYKAGISSAEYLIVNGYNGDALQVMKENKN
CCCCHHHHHHHHHHHEEHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCEEEEEECCCC
VNFFYPKEGISFSMDFMVIPKEAIQKELAHAFINFLLDGKVAAENIEHSLFLSPNLAAYE
EEEEECCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCHHHHH
YLSSELKNNTVLFMPKEVLDKAELIKYLGEKGILYNKAWDKVKAS
HHHHHHCCCEEEEECHHHHCHHHHHHHHCCCCCEECCCHHHHCCC
>Mature Secondary Structure
MKKYFLLLLSLFASLSIFTACQNTLVPELHIMIWSDYIKPEIIEKFQESHHCRVIIDTFD
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEEC
SNESMYAKVKLGAGGYDIIFPSNYFMQIMNQQNMLEPLNKELIPNLKNLDPAYTNKVDRS
CCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LLNLGVPYMVSSAGIVYRQDKVENLEVSWGVFGKSNYKGRMTMLNDVRETLGAALKFLGY
HHHCCCCHHHHCCCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
SANTTQLQQIDQAAQVLIDWKTNLAKFESEQYKAGISSAEYLIVNGYNGDALQVMKENKN
CCCCHHHHHHHHHHHEEHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCEEEEEECCCC
VNFFYPKEGISFSMDFMVIPKEAIQKELAHAFINFLLDGKVAAENIEHSLFLSPNLAAYE
EEEEECCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEECCCHHHHH
YLSSELKNNTVLFMPKEVLDKAELIKYLGEKGILYNKAWDKVKAS
HHHHHHCCCEEEEECHHHHCHHHHHHHHCCCCCEECCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]