Click here to switch to the map view.

The map label for this gene is potB [H]

Identifier: 46446506

GI number: 46446506

Start: 1054716

End: 1055630

Strand: Direct

Name: potB [H]

Synonym: pc0872

Alternate gene names: 46446506

Gene position: 1054716-1055630 (Clockwise)

Preceding gene: 46446505

Following gene: 46446507

Centisome position: 43.68

GC content: 34.97

Gene sequence:

>915_bases
ATGATGGATTTATCTTGGAGCGATGCCAAAAGCTCGAGAATAACAATGAATAGCAAAACTAAAAATGAGTGGGTTTTAAC
CCTACCTTCTTTTATTTGGCTCTTAGTATTTTTTTTGATACCAACCTGTATCATTTATGCTTATGCTTTAAAGCCAAATA
ATATTTATGGAGGAGTTGGCGAGGGGTGGTCTTTAGAAGCAATTAAAGATTTAATCAATCCTAATTATTACGTTTTACTA
TTCCGAACGATTTTTTTGGGAATAGCGACGACGCTATTTTGTTTGCTTTTAGCGTTGCCAGTTGGCTACCAGATGACATT
GATTTCAAAACGAGCTAGACGTTTGCTCATGCTCATGTTAGTTCTTCCTTTTTGGAGTAGTTTTCTCATTCGGATTTTTG
CTTGGAAAATGCTTTTACATCCCGAAGGGTATATTAAAAATTTTCTTGTTTCTATTCACCTGATAGATGCTGATACATCT
CTCCTTTATAATCTAGGTTCAGTCATTTTAGTTATGGTTTACACTTATTTACCTTTTGCCGTTTTTCCTATTTATGCAGC
AGCTTCCAAATTCAATTTTCAGCTATTTGAAGCTGCTATGGATTTAGGAGCCACAAAAAACCAAGCATTTTTTAAAGTGT
TTATTCCTGGAATAGCTAAAGGAATTTTAACAGGAACAATGATGGTTTTCATTTCTGCCGTTGGTGCATATGTAATTCCG
GATCTTGTAGGAGGATTTAGCAGTGAAATGATTGGGAATAAAATCGCTCAAAGAGTCTTTGTTGATCGTCATTTACCCCA
AGCAAGCGCTCTTTCTGCTCTTTTGTCTTTAGTTATCTTTTTACCACTTTTAGCCTATGTTTTTATTTCTACTCGGCAAA
AAAAAATTGAAGTAGAAGTGAGGAACAGAGAATGA

Upstream 100 bases:

>100_bases
GGAGATCGAAAAATTTCTGTCAATCAACAACACAGTCGTTTCCTTCTCGATGAAGCCCCCATTAAATGGAAAGATGTAGT
GTGGATTTGGTGGCATTCAG

Downstream 100 bases:

>100_bases
AAAAAAGTCCATTAAGTTTTTGGATCACAGTTTTTGTTCTCATTTTTTTATACGTTCCCATTCTCGTCTTAATCATTAAT
TCTTTTAATGATTCACGATT

Product: spermidine/putrescine ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MMDLSWSDAKSSRITMNSKTKNEWVLTLPSFIWLLVFFLIPTCIIYAYALKPNNIYGGVGEGWSLEAIKDLINPNYYVLL
FRTIFLGIATTLFCLLLALPVGYQMTLISKRARRLLMLMLVLPFWSSFLIRIFAWKMLLHPEGYIKNFLVSIHLIDADTS
LLYNLGSVILVMVYTYLPFAVFPIYAAASKFNFQLFEAAMDLGATKNQAFFKVFIPGIAKGILTGTMMVFISAVGAYVIP
DLVGGFSSEMIGNKIAQRVFVDRHLPQASALSALLSLVIFLPLLAYVFISTRQKKIEVEVRNRE

Sequences:

>Translated_304_residues
MMDLSWSDAKSSRITMNSKTKNEWVLTLPSFIWLLVFFLIPTCIIYAYALKPNNIYGGVGEGWSLEAIKDLINPNYYVLL
FRTIFLGIATTLFCLLLALPVGYQMTLISKRARRLLMLMLVLPFWSSFLIRIFAWKMLLHPEGYIKNFLVSIHLIDADTS
LLYNLGSVILVMVYTYLPFAVFPIYAAASKFNFQLFEAAMDLGATKNQAFFKVFIPGIAKGILTGTMMVFISAVGAYVIP
DLVGGFSSEMIGNKIAQRVFVDRHLPQASALSALLSLVIFLPLLAYVFISTRQKKIEVEVRNRE
>Mature_304_residues
MMDLSWSDAKSSRITMNSKTKNEWVLTLPSFIWLLVFFLIPTCIIYAYALKPNNIYGGVGEGWSLEAIKDLINPNYYVLL
FRTIFLGIATTLFCLLLALPVGYQMTLISKRARRLLMLMLVLPFWSSFLIRIFAWKMLLHPEGYIKNFLVSIHLIDADTS
LLYNLGSVILVMVYTYLPFAVFPIYAAASKFNFQLFEAAMDLGATKNQAFFKVFIPGIAKGILTGTMMVFISAVGAYVIP
DLVGGFSSEMIGNKIAQRVFVDRHLPQASALSALLSLVIFLPLLAYVFISTRQKKIEVEVRNRE

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=224, Percent_Identity=37.0535714285714, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787369, Length=269, Percent_Identity=33.0855018587361, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787713, Length=237, Percent_Identity=28.6919831223629, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34334; Mature: 34334

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDLSWSDAKSSRITMNSKTKNEWVLTLPSFIWLLVFFLIPTCIIYAYALKPNNIYGGVG
CCCCCCCCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGWSLEAIKDLINPNYYVLLFRTIFLGIATTLFCLLLALPVGYQMTLISKRARRLLMLML
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VLPFWSSFLIRIFAWKMLLHPEGYIKNFLVSIHLIDADTSLLYNLGSVILVMVYTYLPFA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHH
VFPIYAAASKFNFQLFEAAMDLGATKNQAFFKVFIPGIAKGILTGTMMVFISAVGAYVIP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVGGFSSEMIGNKIAQRVFVDRHLPQASALSALLSLVIFLPLLAYVFISTRQKKIEVEV
HHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
RNRE
CCCC
>Mature Secondary Structure
MMDLSWSDAKSSRITMNSKTKNEWVLTLPSFIWLLVFFLIPTCIIYAYALKPNNIYGGVG
CCCCCCCCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGWSLEAIKDLINPNYYVLLFRTIFLGIATTLFCLLLALPVGYQMTLISKRARRLLMLML
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VLPFWSSFLIRIFAWKMLLHPEGYIKNFLVSIHLIDADTSLLYNLGSVILVMVYTYLPFA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHH
VFPIYAAASKFNFQLFEAAMDLGATKNQAFFKVFIPGIAKGILTGTMMVFISAVGAYVIP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVGGFSSEMIGNKIAQRVFVDRHLPQASALSALLSLVIFLPLLAYVFISTRQKKIEVEV
HHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
RNRE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]