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The map label for this gene is lepB [C]

Identifier: 46446130

GI number: 46446130

Start: 634712

End: 636676

Strand: Reverse

Name: lepB [C]

Synonym: pc0496

Alternate gene names: 46446130

Gene position: 636676-634712 (Counterclockwise)

Preceding gene: 46446131

Following gene: 46446126

Centisome position: 26.37

GC content: 35.27

Gene sequence:

>1965_bases
TTGTTAGCGAATCGGTTATCATCTTTGGGTTTTAAACTTCACAAATCGAATAATATGTTTAGTAAACCTCGCCCTTATTC
CTTAGCGAAATCTCACCAAATATTAAAAACTTCCTATACTTTTTATCAGAAAAAACGAAAGCAACTCTCTGCAGATCATT
TAATTCATTTTGAAACTCTCTTAGAATCCCTTGACAAAGTCATTCAAAAAGAAGATCGATTAAAAGCAGATGCTTTCGCT
AAAGAAGCTGAAAAATTTACTCAAATACATTTTAAAAAGAGCTTTCTAGATTATACTTGGGAAATTGGTTTAGCCATTTT
TATTGCATTATTAATTGCCGTTGTAGTTCGTCAAATGTGGTTCGAGCTTTATGAAATCCCCACCGGTTCTATGCGCCCAA
CTTTTAAAGAACAAGACCATCTCTCTGTCACAAAAACAGCCTTTGGTTTAAATATACCATTGGAGACTAATCATTTTTAT
TTTGATCCAAACCTTGTCCAAAGAACAAGTGTGGTAATTTGGTCTGGTGATGGAATTTCTCATCTCGATTCTGACTCTAC
CTTCATGACTATTTTTCCTTATACCAAACGTTATATCAAAAGGTGCATGGGTAAACCTGGAGATATTTTGTATTTTTACG
GAGGTAAAATTTATGGGATAGATCAAGATGGCAATGATTTAAAAGAATTACGAGATAGTCCCTATCTCTCAAAACTAGAT
CATATTCCTTTTACAAACTTTGAAGGCAAGCGAGCTTATACTCAAGATTCCCAACTGAAAATGATTAATCAAGTCGCATT
CGGCCATTTCTCTCTAAATGTTGGACGTTATCGTTTTATGCGTCAATCTATTGCAGGAGAGGTATTCAATGGTAGAGAAT
GGATTAAAGACAATCCTCTTGCTCAGAAAAAAGCACACCGCTCTATTGAAACTTATAGTGATTTATGGGGAATTCGCAAC
ATCGCCATCGCAAGATTATTGACAAAAGATCAGATAGAAAAGTTTACAACATTTTCTTTAAAAGATTTTGGAGAAGGAAT
TCTTTACTTAGAGTTAAGACATACTCCTAGCCTTAGTTATCCTTTACCCATTCTTTCAGATTTTTATGGACCATCAATTG
AAGGTTTTACCACATTAATTCCTCTTGAAGAAAAACATTTGAAAGCTCTTATGGATAATATGTACACCTGCCGTTTTCAT
GTTCAAAATGAAAAAGGAGTACCTTATCGAGTTGAAAATCAGAAATCTCCTTCTCAACACAGTCCTTCGTTTCCCAATGT
TCCTAACGGAACATATGAATTTTATTATGGAAAAGCTCAGCAGATTCATTGGGGTGGTATTTCCACAACACTGCCCTCTA
ATCATCCTCTTTATGATTTCACACCCAATAACGTCCAAAAGCTATTTAATATCGGTATTGAGATGAATAATCAAGTAGAG
CCTAATCAAGCTAAACAAGCTTTTTTTCCTAATAGATATGTGTATTTTCGAGAAGGCGATTTATATGCTATGGGTGGAAA
AGTTTTGGATAAAGAAGATTCAGTTTTACAAAATTTTCATCAAACAGAAAAAAATCGTGAGGAAAAATCTACTGAAGCAA
ACCCTTATATTGCTTTTAAAGACTATGGACCTCCTTTAACCTCTTCTGGTGAATTGGATAAAGAGTTTATTCGTACATTT
GGATTAAAAATCCCTCAAAATCATTATCTCGTTTTGGGTGATAACCACGCCATGAGTCAAGATAGCCGTTTTTTTGGCCC
GATTCCGCAAGCAAATTTACAAGGTGCTCCCTCTCTTATTCTTTGGCCTCCAGGCGATCGCTGGGGTTTCCCAAATCAAA
AACCTTATCCTCTTTTTACATTTCCAAGACTAATTGTTTGGGGTTTAGCGAGCTTGATCGGATTAGCCTGGTGGTTATTT
CGTCATCATAGCCGAACAAAATCTGTGTTCAAAAAATTAGGATGA

Upstream 100 bases:

>100_bases
CTTTTTGATAACCAAGCAATTAAAATCATAAAAAAGAGGCCAAATTAAACTTTGCGAAACGCAAACATATTTTTTAAACT
TCAATTTATAACATTGTCAT

Downstream 100 bases:

>100_bases
AATTGACAAGATATGGGAAATTTTTTTAAAGTTCACAGATTTATTCTATCTTTTTTATTCTATCTTTCTTGAGCAAATAA
ATTCAAAGATAATGGTGAAG

Product: putative signal peptidase I

Products: NA

Alternate protein names: Signal Peptidase

Number of amino acids: Translated: 654; Mature: 654

Protein sequence:

>654_residues
MLANRLSSLGFKLHKSNNMFSKPRPYSLAKSHQILKTSYTFYQKKRKQLSADHLIHFETLLESLDKVIQKEDRLKADAFA
KEAEKFTQIHFKKSFLDYTWEIGLAIFIALLIAVVVRQMWFELYEIPTGSMRPTFKEQDHLSVTKTAFGLNIPLETNHFY
FDPNLVQRTSVVIWSGDGISHLDSDSTFMTIFPYTKRYIKRCMGKPGDILYFYGGKIYGIDQDGNDLKELRDSPYLSKLD
HIPFTNFEGKRAYTQDSQLKMINQVAFGHFSLNVGRYRFMRQSIAGEVFNGREWIKDNPLAQKKAHRSIETYSDLWGIRN
IAIARLLTKDQIEKFTTFSLKDFGEGILYLELRHTPSLSYPLPILSDFYGPSIEGFTTLIPLEEKHLKALMDNMYTCRFH
VQNEKGVPYRVENQKSPSQHSPSFPNVPNGTYEFYYGKAQQIHWGGISTTLPSNHPLYDFTPNNVQKLFNIGIEMNNQVE
PNQAKQAFFPNRYVYFREGDLYAMGGKVLDKEDSVLQNFHQTEKNREEKSTEANPYIAFKDYGPPLTSSGELDKEFIRTF
GLKIPQNHYLVLGDNHAMSQDSRFFGPIPQANLQGAPSLILWPPGDRWGFPNQKPYPLFTFPRLIVWGLASLIGLAWWLF
RHHSRTKSVFKKLG

Sequences:

>Translated_654_residues
MLANRLSSLGFKLHKSNNMFSKPRPYSLAKSHQILKTSYTFYQKKRKQLSADHLIHFETLLESLDKVIQKEDRLKADAFA
KEAEKFTQIHFKKSFLDYTWEIGLAIFIALLIAVVVRQMWFELYEIPTGSMRPTFKEQDHLSVTKTAFGLNIPLETNHFY
FDPNLVQRTSVVIWSGDGISHLDSDSTFMTIFPYTKRYIKRCMGKPGDILYFYGGKIYGIDQDGNDLKELRDSPYLSKLD
HIPFTNFEGKRAYTQDSQLKMINQVAFGHFSLNVGRYRFMRQSIAGEVFNGREWIKDNPLAQKKAHRSIETYSDLWGIRN
IAIARLLTKDQIEKFTTFSLKDFGEGILYLELRHTPSLSYPLPILSDFYGPSIEGFTTLIPLEEKHLKALMDNMYTCRFH
VQNEKGVPYRVENQKSPSQHSPSFPNVPNGTYEFYYGKAQQIHWGGISTTLPSNHPLYDFTPNNVQKLFNIGIEMNNQVE
PNQAKQAFFPNRYVYFREGDLYAMGGKVLDKEDSVLQNFHQTEKNREEKSTEANPYIAFKDYGPPLTSSGELDKEFIRTF
GLKIPQNHYLVLGDNHAMSQDSRFFGPIPQANLQGAPSLILWPPGDRWGFPNQKPYPLFTFPRLIVWGLASLIGLAWWLF
RHHSRTKSVFKKLG
>Mature_654_residues
MLANRLSSLGFKLHKSNNMFSKPRPYSLAKSHQILKTSYTFYQKKRKQLSADHLIHFETLLESLDKVIQKEDRLKADAFA
KEAEKFTQIHFKKSFLDYTWEIGLAIFIALLIAVVVRQMWFELYEIPTGSMRPTFKEQDHLSVTKTAFGLNIPLETNHFY
FDPNLVQRTSVVIWSGDGISHLDSDSTFMTIFPYTKRYIKRCMGKPGDILYFYGGKIYGIDQDGNDLKELRDSPYLSKLD
HIPFTNFEGKRAYTQDSQLKMINQVAFGHFSLNVGRYRFMRQSIAGEVFNGREWIKDNPLAQKKAHRSIETYSDLWGIRN
IAIARLLTKDQIEKFTTFSLKDFGEGILYLELRHTPSLSYPLPILSDFYGPSIEGFTTLIPLEEKHLKALMDNMYTCRFH
VQNEKGVPYRVENQKSPSQHSPSFPNVPNGTYEFYYGKAQQIHWGGISTTLPSNHPLYDFTPNNVQKLFNIGIEMNNQVE
PNQAKQAFFPNRYVYFREGDLYAMGGKVLDKEDSVLQNFHQTEKNREEKSTEANPYIAFKDYGPPLTSSGELDKEFIRTF
GLKIPQNHYLVLGDNHAMSQDSRFFGPIPQANLQGAPSLILWPPGDRWGFPNQKPYPLFTFPRLIVWGLASLIGLAWWLF
RHHSRTKSVFKKLG

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.21.89

Molecular weight: Translated: 75695; Mature: 75695

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLANRLSSLGFKLHKSNNMFSKPRPYSLAKSHQILKTSYTFYQKKRKQLSADHLIHFETL
CCHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LESLDKVIQKEDRLKADAFAKEAEKFTQIHFKKSFLDYTWEIGLAIFIALLIAVVVRQMW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FELYEIPTGSMRPTFKEQDHLSVTKTAFGLNIPLETNHFYFDPNLVQRTSVVIWSGDGIS
HHHHHCCCCCCCCCCCCCCCCEEEEHEEEEECCEECCCEEECCCCCCEEEEEEECCCCCC
HLDSDSTFMTIFPYTKRYIKRCMGKPGDILYFYGGKIYGIDQDGNDLKELRDSPYLSKLD
CCCCCCCEEEECHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCHHHHHHHCCCCHHHHC
HIPFTNFEGKRAYTQDSQLKMINQVAFGHFSLNVGRYRFMRQSIAGEVFNGREWIKDNPL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEECHHHHHHHHHHHHCHHCCCCHHCCCCCC
AQKKAHRSIETYSDLWGIRNIAIARLLTKDQIEKFTTFSLKDFGEGILYLELRHTPSLSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHCCCEEEEEEECCCCCCC
PLPILSDFYGPSIEGFTTLIPLEEKHLKALMDNMYTCRFHVQNEKGVPYRVENQKSPSQH
CCHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCEEECCCCCCCCC
SPSFPNVPNGTYEFYYGKAQQIHWGGISTTLPSNHPLYDFTPNNVQKLFNIGIEMNNQVE
CCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCC
PNQAKQAFFPNRYVYFREGDLYAMGGKVLDKEDSVLQNFHQTEKNREEKSTEANPYIAFK
CCHHHHHCCCCCEEEEECCCEEEECCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
DYGPPLTSSGELDKEFIRTFGLKIPQNHYLVLGDNHAMSQDSRFFGPIPQANLQGAPSLI
CCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEE
LWPPGDRWGFPNQKPYPLFTFPRLIVWGLASLIGLAWWLFRHHSRTKSVFKKLG
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLANRLSSLGFKLHKSNNMFSKPRPYSLAKSHQILKTSYTFYQKKRKQLSADHLIHFETL
CCHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LESLDKVIQKEDRLKADAFAKEAEKFTQIHFKKSFLDYTWEIGLAIFIALLIAVVVRQMW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FELYEIPTGSMRPTFKEQDHLSVTKTAFGLNIPLETNHFYFDPNLVQRTSVVIWSGDGIS
HHHHHCCCCCCCCCCCCCCCCEEEEHEEEEECCEECCCEEECCCCCCEEEEEEECCCCCC
HLDSDSTFMTIFPYTKRYIKRCMGKPGDILYFYGGKIYGIDQDGNDLKELRDSPYLSKLD
CCCCCCCEEEECHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCHHHHHHHCCCCHHHHC
HIPFTNFEGKRAYTQDSQLKMINQVAFGHFSLNVGRYRFMRQSIAGEVFNGREWIKDNPL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEECHHHHHHHHHHHHCHHCCCCHHCCCCCC
AQKKAHRSIETYSDLWGIRNIAIARLLTKDQIEKFTTFSLKDFGEGILYLELRHTPSLSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHCCCEEEEEEECCCCCCC
PLPILSDFYGPSIEGFTTLIPLEEKHLKALMDNMYTCRFHVQNEKGVPYRVENQKSPSQH
CCHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCEEECCCCCCCCC
SPSFPNVPNGTYEFYYGKAQQIHWGGISTTLPSNHPLYDFTPNNVQKLFNIGIEMNNQVE
CCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCC
PNQAKQAFFPNRYVYFREGDLYAMGGKVLDKEDSVLQNFHQTEKNREEKSTEANPYIAFK
CCHHHHHCCCCCEEEEECCCEEEECCEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
DYGPPLTSSGELDKEFIRTFGLKIPQNHYLVLGDNHAMSQDSRFFGPIPQANLQGAPSLI
CCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEE
LWPPGDRWGFPNQKPYPLFTFPRLIVWGLASLIGLAWWLFRHHSRTKSVFKKLG
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phospholipids; Triton X-100 [C]

Metal ions: NA

Kcat value (1/min): 523.8 [C]

Specific activity: NA

Km value (mM): 0.52 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.33 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile}} 0.032 {pro-OmpA-nuclease} 0.0165 {pro-OmpA-nuclease} 1 {Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-CONH2}} [C]

Substrates: NA

Specific reaction: NA

General reaction: Peptide bond hydrolysis [C]

Inhibitor: 1-Ethyl -3-(3-dimethyl aminopropyl) carbodiimide; beta-Lactams; Bromosuccinimide; Carboxyphenanthroline; Cholate; Cu2+; Deoxycholate; Diethyl dicarbonate; Dinitrophenol; Hg2+; Mg2+ most serine peptidases; NEM Cys; Phenyl glyoxal; SDS; Sodiumchloride [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA