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The map label for this gene is cutE [H]

Identifier: 46446034

GI number: 46446034

Start: 537373

End: 538962

Strand: Direct

Name: cutE [H]

Synonym: pc0400

Alternate gene names: 46446034

Gene position: 537373-538962 (Clockwise)

Preceding gene: 46446033

Following gene: 46446035

Centisome position: 22.26

GC content: 37.67

Gene sequence:

>1590_bases
TTGGCGTCGATTGGTGGGTTTGCCTGTTTTTGGCGCATTTTGCTGACAATTTCTTCTAAAAAAGAGCGCTTTTGTTTAGC
AATGGGGTGGTATGCGGCAGTGCAAATGGTGCAGCTTGGATGGTTTGTCTCTCATCCTTATTTTTACATCTATGGAGTGC
TATTTTTTTGTGCATGGTTGATGGGGGCACAATTTGGTCTGCTGGCTTTAGCTATTCAACCTTCTACTCTAAAAAGAGTT
TCTTATTTATTTGGTTTAGCAGGATTATGGGTTTGGTTAGAATGGTCACGTTTATTCATTTTATCAGGTTTATCGTTTAA
TCCAGTAGGCATTGCTTTATCAGGATTCATTTATCCTCTTCAATTTGCTTCGATCGGAGGAGTCTATTTTCTTTCTTTTT
GGGTAATGTTAACCAATTTATGGATGCTAAGAGCCTGGTTTTTTGGATGGAATAAAAGAGAAATAAGTTTAGCGATTGGA
TGTGCTTTGAGTCCTTATTTAATGGGGGGAAGCCATTTTTATTACCACTTAAATCAAATGAACCAAAACACAAGATCCAT
TTCTGCGGTACTTGTGCAACCTGCCCTTCCGATTGAAGAAAATTTGGGATTTCAAAGTGCGGAAGAGGCACGTAACTTTG
TTTTACAAGAATGGCATCAGATTTTATCGACGATGCAAAAACAAAAAGGTCGCCAAATTGATTTGATTGTTTTACCCGAA
TATGTAGTTCCTTATGGAACATATCACCCAGTTTTTCCTTTAGAAAATATCAAGAAGTTGTGGAAAGAGCTATTTGGATT
TGAAGCTTTAAAGTCCCTTGCTCCTTTAATATCTCCTTATGCTTCATTTTTATCGTCCGATCGAGGAAATCAATGGCTCG
TTTCTAACGCTTTTATCGTGAAATCTTTGGCTAATTTATTTCAGTCCTATGTTGTCGTGGGATTTGAAGATAGTGTCTAT
CTTGATGAAGAAAAAACATGTCATGCATCTTATAGTGCTGCCTTTCACTTTTCTCCTGATCAGCGTCCTGCTGAAAGGTA
TGAAAAACGTATACTCGTTCCGATGGGAGAGTATATTCCTTTCGAATTTTGCCGGGAATTAGCTGCCAGGTATGGTATTT
CAGGTTCTTTTACTTGCGGGAAAGGAGCCAAAATTTTTAATGGACCAGTTCCTTATGGAGCAACAATTTGTTATGAGGAA
ACTTACGGCGATTTAATCCGTGAAAATAGACAAAAAGGGGCCGAACTGCTAGTCAACTTGACAAACGATGGTTGGTATCC
TAATTCTAAGCTTCCTAAACAACATTTTGATCATGCGAGATTGCGTACAGTTGAAAATGGAATTCCCTTGATGAGAGCTT
GTAACACCGGAATCACTGGAGCAATCGATTCTTTAGGTCAAGTAATTAATGTATTAGGACCAGATATGATGAAAACACAA
GAAATAGCCGATTCTCTTTATGTACAAGTCCCTGTTTATCATTATAAAACCCTATATGCAAAATGGGGTGACCTGTTTAT
TTTATGCCTAAGTAGTTTGCTTATGATTTGGACAATTGCAGACAAATTTTTTCGATGTTTCTTTTTCTGA

Upstream 100 bases:

>100_bases
AAATACAAATTTAAGCCCTCTTCAGAAATGGGGAGGGTTTTTCTTCTCTTTCCTTATTGTGGCTTTTGGTCAACCTGCTT
GGAGTATATCTTTAGGGCTT

Downstream 100 bases:

>100_bases
ATAAGTCCTTGTATGGGTGAATTATTTGATTAGAATTAATAGAAAAATTTGATGAGTGCCATGATTACTTCCTTGCTTTA
AAACCGATTTATAATTAACA

Product: apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ALP N-acyltransferase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MASIGGFACFWRILLTISSKKERFCLAMGWYAAVQMVQLGWFVSHPYFYIYGVLFFCAWLMGAQFGLLALAIQPSTLKRV
SYLFGLAGLWVWLEWSRLFILSGLSFNPVGIALSGFIYPLQFASIGGVYFLSFWVMLTNLWMLRAWFFGWNKREISLAIG
CALSPYLMGGSHFYYHLNQMNQNTRSISAVLVQPALPIEENLGFQSAEEARNFVLQEWHQILSTMQKQKGRQIDLIVLPE
YVVPYGTYHPVFPLENIKKLWKELFGFEALKSLAPLISPYASFLSSDRGNQWLVSNAFIVKSLANLFQSYVVVGFEDSVY
LDEEKTCHASYSAAFHFSPDQRPAERYEKRILVPMGEYIPFEFCRELAARYGISGSFTCGKGAKIFNGPVPYGATICYEE
TYGDLIRENRQKGAELLVNLTNDGWYPNSKLPKQHFDHARLRTVENGIPLMRACNTGITGAIDSLGQVINVLGPDMMKTQ
EIADSLYVQVPVYHYKTLYAKWGDLFILCLSSLLMIWTIADKFFRCFFF

Sequences:

>Translated_529_residues
MASIGGFACFWRILLTISSKKERFCLAMGWYAAVQMVQLGWFVSHPYFYIYGVLFFCAWLMGAQFGLLALAIQPSTLKRV
SYLFGLAGLWVWLEWSRLFILSGLSFNPVGIALSGFIYPLQFASIGGVYFLSFWVMLTNLWMLRAWFFGWNKREISLAIG
CALSPYLMGGSHFYYHLNQMNQNTRSISAVLVQPALPIEENLGFQSAEEARNFVLQEWHQILSTMQKQKGRQIDLIVLPE
YVVPYGTYHPVFPLENIKKLWKELFGFEALKSLAPLISPYASFLSSDRGNQWLVSNAFIVKSLANLFQSYVVVGFEDSVY
LDEEKTCHASYSAAFHFSPDQRPAERYEKRILVPMGEYIPFEFCRELAARYGISGSFTCGKGAKIFNGPVPYGATICYEE
TYGDLIRENRQKGAELLVNLTNDGWYPNSKLPKQHFDHARLRTVENGIPLMRACNTGITGAIDSLGQVINVLGPDMMKTQ
EIADSLYVQVPVYHYKTLYAKWGDLFILCLSSLLMIWTIADKFFRCFFF
>Mature_528_residues
ASIGGFACFWRILLTISSKKERFCLAMGWYAAVQMVQLGWFVSHPYFYIYGVLFFCAWLMGAQFGLLALAIQPSTLKRVS
YLFGLAGLWVWLEWSRLFILSGLSFNPVGIALSGFIYPLQFASIGGVYFLSFWVMLTNLWMLRAWFFGWNKREISLAIGC
ALSPYLMGGSHFYYHLNQMNQNTRSISAVLVQPALPIEENLGFQSAEEARNFVLQEWHQILSTMQKQKGRQIDLIVLPEY
VVPYGTYHPVFPLENIKKLWKELFGFEALKSLAPLISPYASFLSSDRGNQWLVSNAFIVKSLANLFQSYVVVGFEDSVYL
DEEKTCHASYSAAFHFSPDQRPAERYEKRILVPMGEYIPFEFCRELAARYGISGSFTCGKGAKIFNGPVPYGATICYEET
YGDLIRENRQKGAELLVNLTNDGWYPNSKLPKQHFDHARLRTVENGIPLMRACNTGITGAIDSLGQVINVLGPDMMKTQE
IADSLYVQVPVYHYKTLYAKWGDLFILCLSSLLMIWTIADKFFRCFFF

Specific function: Transfers the fatty acyl group on membrane lipoproteins [H]

COG id: COG0815

COG function: function code M; Apolipoprotein N-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786878, Length=175, Percent_Identity=33.7142857142857, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004563
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 60386; Mature: 60255

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIGGFACFWRILLTISSKKERFCLAMGWYAAVQMVQLGWFVSHPYFYIYGVLFFCAWL
CCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
MGAQFGLLALAIQPSTLKRVSYLFGLAGLWVWLEWSRLFILSGLSFNPVGIALSGFIYPL
HCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QFASIGGVYFLSFWVMLTNLWMLRAWFFGWNKREISLAIGCALSPYLMGGSHFYYHLNQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHCHHHCCCCEEEEEEHHH
NQNTRSISAVLVQPALPIEENLGFQSAEEARNFVLQEWHQILSTMQKQKGRQIDLIVLPE
CCCHHHHHHHEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECH
YVVPYGTYHPVFPLENIKKLWKELFGFEALKSLAPLISPYASFLSSDRGNQWLVSNAFIV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH
KSLANLFQSYVVVGFEDSVYLDEEKTCHASYSAAFHFSPDQRPAERYEKRILVPMGEYIP
HHHHHHHHHHEEEECCCCEEECCCCCCCCCCCEEEECCCCCCHHHHHHCEEECCCCCCCC
FEFCRELAARYGISGSFTCGKGAKIFNGPVPYGATICYEETYGDLIRENRQKGAELLVNL
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHEEEEE
TNDGWYPNSKLPKQHFDHARLRTVENGIPLMRACNTGITGAIDSLGQVINVLGPDMMKTQ
CCCCCCCCCCCCHHHCCHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
EIADSLYVQVPVYHYKTLYAKWGDLFILCLSSLLMIWTIADKFFRCFFF
HHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASIGGFACFWRILLTISSKKERFCLAMGWYAAVQMVQLGWFVSHPYFYIYGVLFFCAWL
CCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
MGAQFGLLALAIQPSTLKRVSYLFGLAGLWVWLEWSRLFILSGLSFNPVGIALSGFIYPL
HCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QFASIGGVYFLSFWVMLTNLWMLRAWFFGWNKREISLAIGCALSPYLMGGSHFYYHLNQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHCHHHCCCCEEEEEEHHH
NQNTRSISAVLVQPALPIEENLGFQSAEEARNFVLQEWHQILSTMQKQKGRQIDLIVLPE
CCCHHHHHHHEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECH
YVVPYGTYHPVFPLENIKKLWKELFGFEALKSLAPLISPYASFLSSDRGNQWLVSNAFIV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH
KSLANLFQSYVVVGFEDSVYLDEEKTCHASYSAAFHFSPDQRPAERYEKRILVPMGEYIP
HHHHHHHHHHEEEECCCCEEECCCCCCCCCCCEEEECCCCCCHHHHHHCEEECCCCCCCC
FEFCRELAARYGISGSFTCGKGAKIFNGPVPYGATICYEETYGDLIRENRQKGAELLVNL
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHEEEEE
TNDGWYPNSKLPKQHFDHARLRTVENGIPLMRACNTGITGAIDSLGQVINVLGPDMMKTQ
CCCCCCCCCCCCHHHCCHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
EIADSLYVQVPVYHYKTLYAKWGDLFILCLSSLLMIWTIADKFFRCFFF
HHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]