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The map label for this gene is rsmB [H]

Identifier: 46446031

GI number: 46446031

Start: 530661

End: 531794

Strand: Reverse

Name: rsmB [H]

Synonym: pc0397

Alternate gene names: 46446031

Gene position: 531794-530661 (Counterclockwise)

Preceding gene: 46446039

Following gene: 46446030

Centisome position: 22.03

GC content: 36.51

Gene sequence:

>1134_bases
ATGACACAAAAAATTCCTTTCCGTGATTACCATTTATTTTCTTTATTAGAAGCCTATGACAGGCAAACTTTACCTCTTGA
TCTTTTCATCAGTCATTATTTTAGAGAACATTCTTCTTTAGGGTCAAAAGATCGAGGGTTTTTAGCAGAAACCATTTATG
CATTAATACGCTGGAAAGGATTGCTAGAATATCTTGCCCCTTCTTCTTCCTGGCAGGAAAAATATATTGTTTACCAAACA
ACAGACTTATCTAAAATGCAGGCTCGTGAAGATATTCCACTTCATGCAAGAGTCAGTTTTCCTTCCCATTTATTTGAATT
ATTCGAAGAATGTTATGGGGTAGAAAAAGCTGTTAATTTATGTTTAATTAACAACACCACTGCCCCTACAACAATTAGAG
TCAATCGAATCAAGACAACGAGAGATTCTTTATTTAAAAGATGGGAAGGTCAATATAAAATCTCCCATACACGTTATTCT
TCAGAAGGAATTATCTTTCATAAGAAAATTAACTTTTTTAGCCTCCCCGAATTTAAAGAAGGCTTGTTCGAAGTACAGGA
CGAAGGAAGTCAACTATTGGCACAGCTTGTCCGGCCTAAACCTGGTGACCAAGTATTAGATTATTGCGCAGGTTCGGGTG
GTAAAACGTTAGCATTTGCTCCTCACATGGAACATCGAGGGCAAATTTATCTTCATGATATTCGCCCTTTTGCTCTTCAA
GAAGCAAAAAAAAGGTTGAAACGAGCAGGCATTCAAAATAGTCAAATTCTTTTACCAAATAGCCCTCAATTATCAAAATT
GAAAAAAAAGATGAATTGGATTTTAGTAGACGCTCCTTGCACAGGGACGGGTACATTACGCCGTAATCCTGACATGAAAT
GGAAATTTGATGAAACCGTTCTTCCTCGCTTGATTGGCCAACAAAGAACAATTTTTGAAAAAGCTTTAAGTTTTTTACAT
CCTGAAGGGCACATTGTATATGGGACCTGCAGCATGTTAAATGCTGAAAATCAGAACCAATTAGAACATTTCATTAAAAC
ATATTCTCTCGAAGTGGAAGGAGAAATATTCCAAACAATCCCAGCTCAGGGGGAAATGGACGGTTTTTTTGGAGTTGTTC
TTAAAAGAGCATAA

Upstream 100 bases:

>100_bases
TGGTTTTTCTCAATACCTAATTCGGTTCACCATTTATCAAACAACAGTTTATGAGATAATTCTATTTAGAATAATCTTTG
CCTTCCTCTATAATCATTTT

Downstream 100 bases:

>100_bases
CTCTATCATATAATGACAGTTTCCATAAATATATACCCTTTAACATATAAAATTTTATTTCTTGTTATGAAAAAAACTAT
CTTTATTGCTTCTCTTTCTT

Product: putative 16S rRNA m5C967 SAM-dependent methyltransferase

Products: NA

Alternate protein names: rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTQKIPFRDYHLFSLLEAYDRQTLPLDLFISHYFREHSSLGSKDRGFLAETIYALIRWKGLLEYLAPSSSWQEKYIVYQT
TDLSKMQAREDIPLHARVSFPSHLFELFEECYGVEKAVNLCLINNTTAPTTIRVNRIKTTRDSLFKRWEGQYKISHTRYS
SEGIIFHKKINFFSLPEFKEGLFEVQDEGSQLLAQLVRPKPGDQVLDYCAGSGGKTLAFAPHMEHRGQIYLHDIRPFALQ
EAKKRLKRAGIQNSQILLPNSPQLSKLKKKMNWILVDAPCTGTGTLRRNPDMKWKFDETVLPRLIGQQRTIFEKALSFLH
PEGHIVYGTCSMLNAENQNQLEHFIKTYSLEVEGEIFQTIPAQGEMDGFFGVVLKRA

Sequences:

>Translated_377_residues
MTQKIPFRDYHLFSLLEAYDRQTLPLDLFISHYFREHSSLGSKDRGFLAETIYALIRWKGLLEYLAPSSSWQEKYIVYQT
TDLSKMQAREDIPLHARVSFPSHLFELFEECYGVEKAVNLCLINNTTAPTTIRVNRIKTTRDSLFKRWEGQYKISHTRYS
SEGIIFHKKINFFSLPEFKEGLFEVQDEGSQLLAQLVRPKPGDQVLDYCAGSGGKTLAFAPHMEHRGQIYLHDIRPFALQ
EAKKRLKRAGIQNSQILLPNSPQLSKLKKKMNWILVDAPCTGTGTLRRNPDMKWKFDETVLPRLIGQQRTIFEKALSFLH
PEGHIVYGTCSMLNAENQNQLEHFIKTYSLEVEGEIFQTIPAQGEMDGFFGVVLKRA
>Mature_376_residues
TQKIPFRDYHLFSLLEAYDRQTLPLDLFISHYFREHSSLGSKDRGFLAETIYALIRWKGLLEYLAPSSSWQEKYIVYQTT
DLSKMQAREDIPLHARVSFPSHLFELFEECYGVEKAVNLCLINNTTAPTTIRVNRIKTTRDSLFKRWEGQYKISHTRYSS
EGIIFHKKINFFSLPEFKEGLFEVQDEGSQLLAQLVRPKPGDQVLDYCAGSGGKTLAFAPHMEHRGQIYLHDIRPFALQE
AKKRLKRAGIQNSQILLPNSPQLSKLKKKMNWILVDAPCTGTGTLRRNPDMKWKFDETVLPRLIGQQRTIFEKALSFLHP
EGHIVYGTCSMLNAENQNQLEHFIKTYSLEVEGEIFQTIPAQGEMDGFFGVVLKRA

Specific function: Unknown

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=261, Percent_Identity=25.2873563218391, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI76150623, Length=261, Percent_Identity=25.2873563218391, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI32698918, Length=177, Percent_Identity=29.9435028248588, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI270288818, Length=257, Percent_Identity=26.0700389105058, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI8922322, Length=257, Percent_Identity=26.0700389105058, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI270288816, Length=260, Percent_Identity=25.3846153846154, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI23199998, Length=260, Percent_Identity=25.3846153846154, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI2367212, Length=288, Percent_Identity=30.9027777777778, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081985, Length=254, Percent_Identity=25.9842519685039, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536757, Length=223, Percent_Identity=29.5964125560538, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115534021, Length=191, Percent_Identity=29.3193717277487, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71998419, Length=191, Percent_Identity=29.3193717277487, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=252, Percent_Identity=24.6031746031746, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI22024126, Length=231, Percent_Identity=25.974025974026, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355201, Length=197, Percent_Identity=31.9796954314721, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 43542; Mature: 43410

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKIPFRDYHLFSLLEAYDRQTLPLDLFISHYFREHSSLGSKDRGFLAETIYALIRWKG
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LLEYLAPSSSWQEKYIVYQTTDLSKMQAREDIPLHARVSFPSHLFELFEECYGVEKAVNL
HHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHCHHHHCEE
CLINNTTAPTTIRVNRIKTTRDSLFKRWEGQYKISHTRYSSEGIIFHKKINFFSLPEFKE
EEEECCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHH
GLFEVQDEGSQLLAQLVRPKPGDQVLDYCAGSGGKTLAFAPHMEHRGQIYLHDIRPFALQ
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCHHHHH
EAKKRLKRAGIQNSQILLPNSPQLSKLKKKMNWILVDAPCTGTGTLRRNPDMKWKFDETV
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEECHHH
LPRLIGQQRTIFEKALSFLHPEGHIVYGTCSMLNAENQNQLEHFIKTYSLEVEGEIFQTI
HHHHHCCHHHHHHHHHHHHCCCCCEEEEEEHCCCCCCHHHHHHHHHHHCEEECCHHHHHC
PAQGEMDGFFGVVLKRA
CCCCCCCCHHHEEEECC
>Mature Secondary Structure 
TQKIPFRDYHLFSLLEAYDRQTLPLDLFISHYFREHSSLGSKDRGFLAETIYALIRWKG
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LLEYLAPSSSWQEKYIVYQTTDLSKMQAREDIPLHARVSFPSHLFELFEECYGVEKAVNL
HHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHCHHHHCEE
CLINNTTAPTTIRVNRIKTTRDSLFKRWEGQYKISHTRYSSEGIIFHKKINFFSLPEFKE
EEEECCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHH
GLFEVQDEGSQLLAQLVRPKPGDQVLDYCAGSGGKTLAFAPHMEHRGQIYLHDIRPFALQ
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCEEEEECCHHHHH
EAKKRLKRAGIQNSQILLPNSPQLSKLKKKMNWILVDAPCTGTGTLRRNPDMKWKFDETV
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEECHHH
LPRLIGQQRTIFEKALSFLHPEGHIVYGTCSMLNAENQNQLEHFIKTYSLEVEGEIFQTI
HHHHHCCHHHHHHHHHHHHCCCCCEEEEEEHCCCCCCHHHHHHHHHHHCEEECCHHHHHC
PAQGEMDGFFGVVLKRA
CCCCCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]