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The map label for this gene is alkB [H]

Identifier: 46445920

GI number: 46445920

Start: 397258

End: 397926

Strand: Direct

Name: alkB [H]

Synonym: pc0286

Alternate gene names: 46445920

Gene position: 397258-397926 (Clockwise)

Preceding gene: 46445918

Following gene: 46445925

Centisome position: 16.45

GC content: 37.37

Gene sequence:

>669_bases
TTGAATACCTATGTATTTTTCATGATTATGAAAGAAGATTTATTTGAAAATCAAAGAAAAGATATGATTTTAGGTCAAGG
TGCTATTTTGTTTGCGGGTTTGGCCAAAAAAATAGACAAATCTTTGTTATCTTCAGTACAGGAAATTACTCAATTAGCAC
CGTTTCGTCATATGAAAACATCTGGAGGATTTGATCTATCTGTCGCTATGACAAATTGTGGTTTGTTAGGATGGGTGACA
GATGAAGCGGGTTACCGCTATCAATCCTTTGATCCTTTAAGCGGGCTTGTATGGCCAAAGATTCCACCTCTTTTTTTAGA
ACTTGCTTTAGAAGCTGCTGAAAGGGCTGGTTATTCTTCTTTTGTTCCTAGCGCTTGTTTGATTAATCGCTATGTTCCCG
GAGCGAAAATGTCTCTTCATCAGGACAAAGATGAAGACGATTTGGATTCACCTATTGTTTCTGTTTCACTCGGCTTGCCT
GCTACTTTTCAATTTGGAGGATTCAATCGTACCGATCCTCTCCAAAAATTATTACTTATACATGGAGATGTAGTTGTTTG
GGGAGGTAAACTACGGTTGGCTTACCATGGTATTCTTCCTTTAAAAAGTGGGCATCATCATTTAACGGGATCGACGCGTA
TTAATTTAACATTTCGCAAAGTTTTTTAA

Upstream 100 bases:

>100_bases
TAATTAAGAAAACTAAATAGCGCATACTAGTATAACTCATCAATGCAATGACAATAAGCCCTAATAAAACGCCTATCCAT
TGACCCATATTTAAAGACAT

Downstream 100 bases:

>100_bases
TTTTTTAGTTTCCGTGTAAATTATTTCGACGATTTTTTAAGCATCCATCAATCTAACAAAGAATCTATTTTTAATGCCTG
TCTTGTTCGCTTTAGGCCCA

Product: alkylated DNA repair protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNTYVFFMIMKEDLFENQRKDMILGQGAILFAGLAKKIDKSLLSSVQEITQLAPFRHMKTSGGFDLSVAMTNCGLLGWVT
DEAGYRYQSFDPLSGLVWPKIPPLFLELALEAAERAGYSSFVPSACLINRYVPGAKMSLHQDKDEDDLDSPIVSVSLGLP
ATFQFGGFNRTDPLQKLLLIHGDVVVWGGKLRLAYHGILPLKSGHHHLTGSTRINLTFRKVF

Sequences:

>Translated_222_residues
MNTYVFFMIMKEDLFENQRKDMILGQGAILFAGLAKKIDKSLLSSVQEITQLAPFRHMKTSGGFDLSVAMTNCGLLGWVT
DEAGYRYQSFDPLSGLVWPKIPPLFLELALEAAERAGYSSFVPSACLINRYVPGAKMSLHQDKDEDDLDSPIVSVSLGLP
ATFQFGGFNRTDPLQKLLLIHGDVVVWGGKLRLAYHGILPLKSGHHHLTGSTRINLTFRKVF
>Mature_222_residues
MNTYVFFMIMKEDLFENQRKDMILGQGAILFAGLAKKIDKSLLSSVQEITQLAPFRHMKTSGGFDLSVAMTNCGLLGWVT
DEAGYRYQSFDPLSGLVWPKIPPLFLELALEAAERAGYSSFVPSACLINRYVPGAKMSLHQDKDEDDLDSPIVSVSLGLP
ATFQFGGFNRTDPLQKLLLIHGDVVVWGGKLRLAYHGILPLKSGHHHLTGSTRINLTFRKVF

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Escherichia coli, GI1788541, Length=198, Percent_Identity=57.0707070707071, Blast_Score=235, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24662; Mature: 24662

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTYVFFMIMKEDLFENQRKDMILGQGAILFAGLAKKIDKSLLSSVQEITQLAPFRHMKT
CCEEEEEEEEEHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
SGGFDLSVAMTNCGLLGWVTDEAGYRYQSFDPLSGLVWPKIPPLFLELALEAAERAGYSS
CCCCEEEEEECCCCEEEEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
FVPSACLINRYVPGAKMSLHQDKDEDDLDSPIVSVSLGLPATFQFGGFNRTDPLQKLLLI
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHH
HGDVVVWGGKLRLAYHGILPLKSGHHHLTGSTRINLTFRKVF
CCCEEEECCEEEEEEECEEECCCCCEEECCCEEEEEEEEECC
>Mature Secondary Structure
MNTYVFFMIMKEDLFENQRKDMILGQGAILFAGLAKKIDKSLLSSVQEITQLAPFRHMKT
CCEEEEEEEEEHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
SGGFDLSVAMTNCGLLGWVTDEAGYRYQSFDPLSGLVWPKIPPLFLELALEAAERAGYSS
CCCCEEEEEECCCCEEEEEECCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
FVPSACLINRYVPGAKMSLHQDKDEDDLDSPIVSVSLGLPATFQFGGFNRTDPLQKLLLI
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHH
HGDVVVWGGKLRLAYHGILPLKSGHHHLTGSTRINLTFRKVF
CCCEEEECCEEEEEEECEEECCCCCEEECCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1904855 [H]