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The map label for this gene is gcvH [H]

Identifier: 46445916

GI number: 46445916

Start: 393695

End: 394063

Strand: Direct

Name: gcvH [H]

Synonym: pc0282

Alternate gene names: 46445916

Gene position: 393695-394063 (Clockwise)

Preceding gene: 46445915

Following gene: 46445917

Centisome position: 16.31

GC content: 37.4

Gene sequence:

>369_bases
ATGAAATATACAGATTCTCATGAATGGGTTGTTTTAGAATCGACTGATGTAGCTCGTATTGGTGTGACGCAGTTTGCGCA
AAAAGAACTCGGTGATATTGTTTATGTTGAACTGCCAACTCTACATAAAAAAGTTACTGCTGGACAGGAGGTGGTGGTTT
TAGAGTCTACTAAAGCGGCCGCTGATGTTTATTCTCCTGTTAGTGGAGAAATCGTTGAAGTGAATCAAAGTTTATCTACT
CAGCCTGAACTCATTAATCAGTCTTCCGAAAAAGAAGGATGGTTATTTAAACTTCGTTTATCTAATTCCTCAGAATTAGA
CTTATTAATGGATGAAAAGGATTATCGTGCGATGTTAAACGGAGCATAA

Upstream 100 bases:

>100_bases
TAGATACACCACTCAAAATCAAACAAGATATTAAAATACAAATTCGGCAAACACAATGCCACGCTGAAGTGGTTCAATTG
CCTTTTATTAGGAAAAACAG

Downstream 100 bases:

>100_bases
ATGGATTTTATTTCAAATAAAACTCCTCAAATTGAAGCCATGTTAACTGAAATTGGGATTCAAAATGTTGAAGAATTATT
TAAATCAATTCCTTCTTCGT

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MKYTDSHEWVVLESTDVARIGVTQFAQKELGDIVYVELPTLHKKVTAGQEVVVLESTKAAADVYSPVSGEIVEVNQSLST
QPELINQSSEKEGWLFKLRLSNSSELDLLMDEKDYRAMLNGA

Sequences:

>Translated_122_residues
MKYTDSHEWVVLESTDVARIGVTQFAQKELGDIVYVELPTLHKKVTAGQEVVVLESTKAAADVYSPVSGEIVEVNQSLST
QPELINQSSEKEGWLFKLRLSNSSELDLLMDEKDYRAMLNGA
>Mature_122_residues
MKYTDSHEWVVLESTDVARIGVTQFAQKELGDIVYVELPTLHKKVTAGQEVVVLESTKAAADVYSPVSGEIVEVNQSLST
QPELINQSSEKEGWLFKLRLSNSSELDLLMDEKDYRAMLNGA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=114, Percent_Identity=47.3684210526316, Blast_Score=119, Evalue=6e-28,
Organism=Homo sapiens, GI49574537, Length=114, Percent_Identity=47.3684210526316, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=47.0588235294118, Blast_Score=122, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=40.7079646017699, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=40.7079646017699, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=32.7868852459016, Blast_Score=91, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=43.6974789915966, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13588; Mature: 13588

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTDSHEWVVLESTDVARIGVTQFAQKELGDIVYVELPTLHKKVTAGQEVVVLESTKAA
CCCCCCCCEEEEECCCCEEHHHHHHHHHHCCCEEEEECCCHHHHHCCCCEEEEEECCCCC
ADVYSPVSGEIVEVNQSLSTQPELINQSSEKEGWLFKLRLSNSSELDLLMDEKDYRAMLN
HHHCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHEEC
GA
CC
>Mature Secondary Structure
MKYTDSHEWVVLESTDVARIGVTQFAQKELGDIVYVELPTLHKKVTAGQEVVVLESTKAA
CCCCCCCCEEEEECCCCEEHHHHHHHHHHCCCEEEEECCCHHHHHCCCCEEEEEECCCCC
ADVYSPVSGEIVEVNQSLSTQPELINQSSEKEGWLFKLRLSNSSELDLLMDEKDYRAMLN
HHHCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHEEC
GA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]