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The map label for this gene is pgk [H]

Identifier: 46445873

GI number: 46445873

Start: 335314

End: 336522

Strand: Direct

Name: pgk [H]

Synonym: pc0239

Alternate gene names: 46445873

Gene position: 335314-336522 (Clockwise)

Preceding gene: 46445872

Following gene: 46445874

Centisome position: 13.89

GC content: 38.46

Gene sequence:

>1209_bases
GTGAGGGTGAAGGATAAATTATCGATTCGCGATTTAAATTTGAAAGACAAGAAAGCCTTAATTCGTGTCGATTTTAACGT
CCCTTTAGAAAAAGGTTTGATTACAGATGATAGTCGTATTCAAGCCTCTCTTCCCACAATTCAATATGTTTTAGATCAAG
AAGGAGCCGTTATTTTGATGAGCCATCTCGGTCGTCCTAAAGGTTTGTTTGATTCTAAATTTTCGTTGGCTCCTTGTGCT
AAACGTTTATCAGAATTATTAAATAAGCCTGTAAAAATGGCTGCAGATTGTTGTGGATTAGACGTTGAACAGATGGTTCG
TCAGCTAAAATCAGGAGAAGTTTTATTGCTTGAAAATTTACGTTTTCACAAAGGCGAAGAAAGGCCTGATGATGAACCTA
ATTTTACAAGTGCTTTGTCAGAGTTGGGAGATGTTTATATCAATGATGCTTTTGGAACTGCTCATCGTGCTCATGCTTCG
ACAACAAAAATTGCAAAATTTTTTCCCAATCGTGCAGCAGCGGGTTTGTTACTTGAAAAGGAAATTTCTTATCTAGGTTG
TGCTTTAACGAATCCTAAACGTCCTTTTTGTGCAGTGTTAGGAGGTGCAAAAATTTCCACAAAGTTTAAAGTCATCGAAG
CTTTACAGTCTAAAGCAGATGTTTTATTAATAGGGGGAGCGATGGCTTTCACTTTTTTCAAATCTGAAGGAATACCTATT
GGGGATTCGCTTTACGAAAGCGAATTCGTTGCTGTCGCTCGTGAATTGTTAGGTGTTGAGACACAGTCTCGCTGCCGCCT
TTTACTACCCATAGACTTGGTCATTGCTAAAAAAATGGATGAATCCTTCACTGAAACGAGAATTATTTCAGTTAAAGAAG
GAATTCCTGATGGGTATCAAGGGGTAGATATTGGGCCAGACACTATTAAACTTTATCAAGAAGAATTGAACAGAGCAGCT
ACGATTTTTTGGAACGGCCCTTTAGGAGTATTTGAAGTTCCTGCTTTTTCTAAAGGGACTCAAACTATTGCTCAAACTCT
TGCTGTTGTCAACGCGATTACTATTGTTGGGGGAGGGGATTCCTTAGCTGCTATAGAACAAGCTGGGGTTTCTAAACAAA
TAAACCATCTTTCAACTGGAGGGGGTGCCTCTTTAGAATATATAGAATTTGGGCAATTACCTGGGATTGAAGCTCTATCA
GATAAATAA

Upstream 100 bases:

>100_bases
GAAATAGAACTTTCTTCATTAGTCTTTTGTCTGTTAATAAGAAACTTGACAGAACTTTTCGTTTAAGTTATTAACTCATT
TCATAAATGAATTAAGGAGG

Downstream 100 bases:

>100_bases
ATCTTAGAGTTCGAATTTATTTGAACTACAGCTAGTCAAAAAAATATAACACTGCTAAGAAGTTTAATTTTTAACTCTCG
GTGTGATATAGCTATCCTTT

Product: 3-phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MRVKDKLSIRDLNLKDKKALIRVDFNVPLEKGLITDDSRIQASLPTIQYVLDQEGAVILMSHLGRPKGLFDSKFSLAPCA
KRLSELLNKPVKMAADCCGLDVEQMVRQLKSGEVLLLENLRFHKGEERPDDEPNFTSALSELGDVYINDAFGTAHRAHAS
TTKIAKFFPNRAAAGLLLEKEISYLGCALTNPKRPFCAVLGGAKISTKFKVIEALQSKADVLLIGGAMAFTFFKSEGIPI
GDSLYESEFVAVARELLGVETQSRCRLLLPIDLVIAKKMDESFTETRIISVKEGIPDGYQGVDIGPDTIKLYQEELNRAA
TIFWNGPLGVFEVPAFSKGTQTIAQTLAVVNAITIVGGGDSLAAIEQAGVSKQINHLSTGGGASLEYIEFGQLPGIEALS
DK

Sequences:

>Translated_402_residues
MRVKDKLSIRDLNLKDKKALIRVDFNVPLEKGLITDDSRIQASLPTIQYVLDQEGAVILMSHLGRPKGLFDSKFSLAPCA
KRLSELLNKPVKMAADCCGLDVEQMVRQLKSGEVLLLENLRFHKGEERPDDEPNFTSALSELGDVYINDAFGTAHRAHAS
TTKIAKFFPNRAAAGLLLEKEISYLGCALTNPKRPFCAVLGGAKISTKFKVIEALQSKADVLLIGGAMAFTFFKSEGIPI
GDSLYESEFVAVARELLGVETQSRCRLLLPIDLVIAKKMDESFTETRIISVKEGIPDGYQGVDIGPDTIKLYQEELNRAA
TIFWNGPLGVFEVPAFSKGTQTIAQTLAVVNAITIVGGGDSLAAIEQAGVSKQINHLSTGGGASLEYIEFGQLPGIEALS
DK
>Mature_402_residues
MRVKDKLSIRDLNLKDKKALIRVDFNVPLEKGLITDDSRIQASLPTIQYVLDQEGAVILMSHLGRPKGLFDSKFSLAPCA
KRLSELLNKPVKMAADCCGLDVEQMVRQLKSGEVLLLENLRFHKGEERPDDEPNFTSALSELGDVYINDAFGTAHRAHAS
TTKIAKFFPNRAAAGLLLEKEISYLGCALTNPKRPFCAVLGGAKISTKFKVIEALQSKADVLLIGGAMAFTFFKSEGIPI
GDSLYESEFVAVARELLGVETQSRCRLLLPIDLVIAKKMDESFTETRIISVKEGIPDGYQGVDIGPDTIKLYQEELNRAA
TIFWNGPLGVFEVPAFSKGTQTIAQTLAVVNAITIVGGGDSLAAIEQAGVSKQINHLSTGGGASLEYIEFGQLPGIEALS
DK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=420, Percent_Identity=42.1428571428571, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI31543397, Length=420, Percent_Identity=42.1428571428571, Blast_Score=332, Evalue=4e-91,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=42.4623115577889, Blast_Score=300, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI17508823, Length=418, Percent_Identity=44.2583732057416, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6319857, Length=420, Percent_Identity=46.1904761904762, Blast_Score=349, Evalue=4e-97,
Organism=Drosophila melanogaster, GI17136394, Length=414, Percent_Identity=42.512077294686, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=39.8550724637681, Blast_Score=301, Evalue=7e-82,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 43630; Mature: 43630

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKDKLSIRDLNLKDKKALIRVDFNVPLEKGLITDDSRIQASLPTIQYVLDQEGAVILM
CCCCCCCCEECCCCCCCEEEEEEECCCCHHCCCCCCCCCCEECCCCEEEEECCCCCEEEE
SHLGRPKGLFDSKFSLAPCAKRLSELLNKPVKMAADCCGLDVEQMVRQLKSGEVLLLENL
ECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECC
RFHKGEERPDDEPNFTSALSELGDVYINDAFGTAHRAHASTTKIAKFFPNRAAAGLLLEK
CCCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
EISYLGCALTNPKRPFCAVLGGAKISTKFKVIEALQSKADVLLIGGAMAFTFFKSEGIPI
HHHHHEEEECCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEECCHHEEEEECCCCCCC
GDSLYESEFVAVARELLGVETQSRCRLLLPIDLVIAKKMDESFTETRIISVKEGIPDGYQ
CCHHHHHHHHHHHHHHHCCCCHHCCEEEEEHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
GVDIGPDTIKLYQEELNRAATIFWNGPLGVFEVPAFSKGTQTIAQTLAVVNAITIVGGGD
CCCCCHHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHEEEEECCC
SLAAIEQAGVSKQINHLSTGGGASLEYIEFGQLPGIEALSDK
CHHHHHHHCHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCC
>Mature Secondary Structure
MRVKDKLSIRDLNLKDKKALIRVDFNVPLEKGLITDDSRIQASLPTIQYVLDQEGAVILM
CCCCCCCCEECCCCCCCEEEEEEECCCCHHCCCCCCCCCCEECCCCEEEEECCCCCEEEE
SHLGRPKGLFDSKFSLAPCAKRLSELLNKPVKMAADCCGLDVEQMVRQLKSGEVLLLENL
ECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEECC
RFHKGEERPDDEPNFTSALSELGDVYINDAFGTAHRAHASTTKIAKFFPNRAAAGLLLEK
CCCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
EISYLGCALTNPKRPFCAVLGGAKISTKFKVIEALQSKADVLLIGGAMAFTFFKSEGIPI
HHHHHEEEECCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEECCHHEEEEECCCCCCC
GDSLYESEFVAVARELLGVETQSRCRLLLPIDLVIAKKMDESFTETRIISVKEGIPDGYQ
CCHHHHHHHHHHHHHHHCCCCHHCCEEEEEHHHHHHHHHHHHHHHEEEEEECCCCCCCCC
GVDIGPDTIKLYQEELNRAATIFWNGPLGVFEVPAFSKGTQTIAQTLAVVNAITIVGGGD
CCCCCHHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHEEEEECCC
SLAAIEQAGVSKQINHLSTGGGASLEYIEFGQLPGIEALSDK
CHHHHHHHCHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]