Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 46445872

GI number: 46445872

Start: 334627

End: 335220

Strand: Direct

Name: hisH

Synonym: pc0238

Alternate gene names: 46445872

Gene position: 334627-335220 (Clockwise)

Preceding gene: 46445871

Following gene: 46445873

Centisome position: 13.86

GC content: 37.37

Gene sequence:

>594_bases
ATGCTGATAGGTATATTAGCATTACAGGGAGATTTCTTTAAACATCAAGAAATGCTTCATTCTCTTGGTATAGAAACGAT
CCAAGTTAAAACTCGAAATGAGTTAGATTTTTGTGATGCTCTTATTATTCCTGGTGGGGAATCTACTGTGATGATGCGAC
AACTTGAAACAACAAATCTTAAAGAGCTATTAGTTCATTTTGCGATCCATAAACCTGTTTTTGGAACTTGTGCTGGCCTT
ATTTTAATGTCTTCTCACGTTCAAAATTCTGCAATGATGCCGCTTGGACTGTTACATATTGCTGTCGAACGAAATGCGTT
TGGGCGGCAAGTCGATTCTTTTCAAGTGGATGTGTCTGTTTATTTAAAACCAGGAGACGAAATATGTTTTCCTGCTTTTT
TTATTCGAGCTCCACGTATTCGAACAAGTGAAACTCCCGTGCAAATTCTTGCTTCTTATGAAGGGGAGCCTATTTTGGTT
CGGCAAGGGCATCATTTAGGAGCATCGTTTCATCCGGAGTTAACAGTCAACCCTTCTATTCATCTTTATTTTCTTGAAAT
GGTCAAAGAAAACTTAGAAAATCATAAGAAATAG

Upstream 100 bases:

>100_bases
ATTAGCTCAATTTTCGATGGATCTTCTTGATGCGATGAAAGGGATTGATATTAGACAACTTAAACGAGAAGATTTGATGG
CTCAAAGAGGTTGGTAGAGC

Downstream 100 bases:

>100_bases
AACTTTCTTCATTAGTCTTTTGTCTGTTAATAAGAAACTTGACAGAACTTTTCGTTTAAGTTATTAACTCATTTCATAAA
TGAATTAAGGAGGGTGAGGG

Product: putative glutamine amidotransferase

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MLIGILALQGDFFKHQEMLHSLGIETIQVKTRNELDFCDALIIPGGESTVMMRQLETTNLKELLVHFAIHKPVFGTCAGL
ILMSSHVQNSAMMPLGLLHIAVERNAFGRQVDSFQVDVSVYLKPGDEICFPAFFIRAPRIRTSETPVQILASYEGEPILV
RQGHHLGASFHPELTVNPSIHLYFLEMVKENLENHKK

Sequences:

>Translated_197_residues
MLIGILALQGDFFKHQEMLHSLGIETIQVKTRNELDFCDALIIPGGESTVMMRQLETTNLKELLVHFAIHKPVFGTCAGL
ILMSSHVQNSAMMPLGLLHIAVERNAFGRQVDSFQVDVSVYLKPGDEICFPAFFIRAPRIRTSETPVQILASYEGEPILV
RQGHHLGASFHPELTVNPSIHLYFLEMVKENLENHKK
>Mature_197_residues
MLIGILALQGDFFKHQEMLHSLGIETIQVKTRNELDFCDALIIPGGESTVMMRQLETTNLKELLVHFAIHKPVFGTCAGL
ILMSSHVQNSAMMPLGLLHIAVERNAFGRQVDSFQVDVSVYLKPGDEICFPAFFIRAPRIRTSETPVQILASYEGEPILV
RQGHHLGASFHPELTVNPSIHLYFLEMVKENLENHKK

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=213, Percent_Identity=40.8450704225352, Blast_Score=124, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6321048, Length=220, Percent_Identity=38.6363636363636, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323995, Length=220, Percent_Identity=37.7272727272727, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_PARUW (Q6MEN7)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007237.1
- ProteinModelPortal:   Q6MEN7
- SMR:   Q6MEN7
- STRING:   Q6MEN7
- GeneID:   2780709
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0238
- NMPDR:   fig|264201.1.peg.238
- eggNOG:   COG0311
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- PhylomeDB:   Q6MEN7
- BioCyc:   CPRO264201:PC0238-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 22159; Mature: 22159

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 77-77 ACT_SITE 171-171 ACT_SITE 173-173 BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGILALQGDFFKHQEMLHSLGIETIQVKTRNELDFCDALIIPGGESTVMMRQLETTNL
CEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCEEEEEECCCCCH
KELLVHFAIHKPVFGTCAGLILMSSHVQNSAMMPLGLLHIAVERNAFGRQVDSFQVDVSV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEE
YLKPGDEICFPAFFIRAPRIRTSETPVQILASYEGEPILVRQGHHLGASFHPELTVNPSI
EECCCCCEEHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEEECCCE
HLYFLEMVKENLENHKK
EEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MLIGILALQGDFFKHQEMLHSLGIETIQVKTRNELDFCDALIIPGGESTVMMRQLETTNL
CEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCEEEEEECCCCCH
KELLVHFAIHKPVFGTCAGLILMSSHVQNSAMMPLGLLHIAVERNAFGRQVDSFQVDVSV
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEE
YLKPGDEICFPAFFIRAPRIRTSETPVQILASYEGEPILVRQGHHLGASFHPELTVNPSI
EECCCCCEEHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCEEECCCE
HLYFLEMVKENLENHKK
EEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA