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The map label for this gene is rho [H]

Identifier: 46445855

GI number: 46445855

Start: 313572

End: 314957

Strand: Reverse

Name: rho [H]

Synonym: pc0221

Alternate gene names: 46445855

Gene position: 314957-313572 (Counterclockwise)

Preceding gene: 46445856

Following gene: 46445854

Centisome position: 13.04

GC content: 39.11

Gene sequence:

>1386_bases
ATGGACCCAGAGAACAAAGAGAACACTTCAGTTGATACCTCGTTGACATGTGAGGACAATTCAGTTGATAAGCAAAACCC
TGAACAATATCATCAACAAATCGTTCAGGAAAGATCTCAACAACCTGAGATAATTACTAAAATTGCCGATATCCAACGGA
TGAATATTGATCAGCTCAATCAATATGGCAAAAAAATTGGCCTTAAACACCTTGGCTCTTTAACTAAGTCTCAGATGGTA
TTTGAAATTGTCAAAGCAATCTCTGAAAATCCAAATGAAGTTTTATATGGAGAAGGTGTTTTAGAAATTCTTCCAGACGG
TTTCGGTTTCCTTCGTTCTCCTAACTATAATTATCTCCCTTCGGCTGAAGACATTTATGTTTCTCCTGCACAAATTCGGC
GTTTTGATTTAAAAAAGGGAGATACTCTTTGTGGAACAATTCGACCACCAAAGGATAAAGAGAAATATTTTGCATTACTC
AAAGTCGATAAAATCAATGGTCGACCACCAGAAAAAGCAAAAGAGCGAATCTTATTTGAAAATTTGACCCCGCTTTATCC
TAATGAACGAATGGTGATGGAAACATCCAAGGAAAAGTTATCCACACGAGTATTAGACCTAGCCGCTCCTGTTGGTAAAG
GTCAGAGAGGTTTAATCGTTGCTCCACCAAGATCAGGTAAAACCATTCTTTTACAAAATATTGCCAACGCGATCGCTTGT
AACAATCCTGAAATTGTACTAATCGTTTTGCTTATCGATGAACGCCCCGAAGAAGTAACAGACATGCAACGTATTGTGCG
CGGCGAAGTCATTTCTTCCACTTTTGATGAACCTCCTGAAAGACACGTTCAAGTCGCCGAAATGGCGATTGAAAAAGCCC
GTCGCTTAGTGGAACATGGAAATGATGTTGTCATTCTTTTAGACTCGATTACTCGTTTAGCGCGCGCTTATAATACTATC
CAACCACATTCTGGTAAAATCTTAACAGGTGGTATTGATGCAAATGCTTTACATAAACCTAAACGTTTCTTTGGAGCTGC
TCGTAATATTGAGCAAGGCGGTTCTCTAACAATCATTGCAACGGCTTTGATCGACACAGGTTCGCGCATGGATGAAGTCA
TTTTTGAAGAATTCAAAGGAACTGGTAATATGGAATTAGTGCTCGATCGCCGCTTAGCTGATCGTCGCATTTATCCAGCT
ATTGATTTGATCAAGAGTGGAACTCGTAAAGAAGAGTTACTTTATCATCCTAATGAATTAGAAAAAATTTATCTACTTCG
TCAAGCTGTTGCCGACTTAGCTCCGGGAGATGCAATGAACCTTTTATTAGGGCGCTTGAAAAAAACAGGTAGCAATGTGG
AATTTCTTCTCTCCATGAAAGATTAA

Upstream 100 bases:

>100_bases
TGGAACTTTATCAGAACTTCAACAATCCCTGAGAGAATTATACCAAGAATTAAAAATATATATTTAACTATTAACCATTT
ACATTGGAGTCTTATTTTTA

Downstream 100 bases:

>100_bases
TCTCTCAAAGGGTCTGAACTTTAACTTCAGGCCCTTTTCATAAAATTTCTCTCTACAAATAACGATTGTTTACATTCGAT
TTTGAAACAAAATTAATCGA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MDPENKENTSVDTSLTCEDNSVDKQNPEQYHQQIVQERSQQPEIITKIADIQRMNIDQLNQYGKKIGLKHLGSLTKSQMV
FEIVKAISENPNEVLYGEGVLEILPDGFGFLRSPNYNYLPSAEDIYVSPAQIRRFDLKKGDTLCGTIRPPKDKEKYFALL
KVDKINGRPPEKAKERILFENLTPLYPNERMVMETSKEKLSTRVLDLAAPVGKGQRGLIVAPPRSGKTILLQNIANAIAC
NNPEIVLIVLLIDERPEEVTDMQRIVRGEVISSTFDEPPERHVQVAEMAIEKARRLVEHGNDVVILLDSITRLARAYNTI
QPHSGKILTGGIDANALHKPKRFFGAARNIEQGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLADRRIYPA
IDLIKSGTRKEELLYHPNELEKIYLLRQAVADLAPGDAMNLLLGRLKKTGSNVEFLLSMKD

Sequences:

>Translated_461_residues
MDPENKENTSVDTSLTCEDNSVDKQNPEQYHQQIVQERSQQPEIITKIADIQRMNIDQLNQYGKKIGLKHLGSLTKSQMV
FEIVKAISENPNEVLYGEGVLEILPDGFGFLRSPNYNYLPSAEDIYVSPAQIRRFDLKKGDTLCGTIRPPKDKEKYFALL
KVDKINGRPPEKAKERILFENLTPLYPNERMVMETSKEKLSTRVLDLAAPVGKGQRGLIVAPPRSGKTILLQNIANAIAC
NNPEIVLIVLLIDERPEEVTDMQRIVRGEVISSTFDEPPERHVQVAEMAIEKARRLVEHGNDVVILLDSITRLARAYNTI
QPHSGKILTGGIDANALHKPKRFFGAARNIEQGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLADRRIYPA
IDLIKSGTRKEELLYHPNELEKIYLLRQAVADLAPGDAMNLLLGRLKKTGSNVEFLLSMKD
>Mature_461_residues
MDPENKENTSVDTSLTCEDNSVDKQNPEQYHQQIVQERSQQPEIITKIADIQRMNIDQLNQYGKKIGLKHLGSLTKSQMV
FEIVKAISENPNEVLYGEGVLEILPDGFGFLRSPNYNYLPSAEDIYVSPAQIRRFDLKKGDTLCGTIRPPKDKEKYFALL
KVDKINGRPPEKAKERILFENLTPLYPNERMVMETSKEKLSTRVLDLAAPVGKGQRGLIVAPPRSGKTILLQNIANAIAC
NNPEIVLIVLLIDERPEEVTDMQRIVRGEVISSTFDEPPERHVQVAEMAIEKARRLVEHGNDVVILLDSITRLARAYNTI
QPHSGKILTGGIDANALHKPKRFFGAARNIEQGGSLTIIATALIDTGSRMDEVIFEEFKGTGNMELVLDRRLADRRIYPA
IDLIKSGTRKEELLYHPNELEKIYLLRQAVADLAPGDAMNLLLGRLKKTGSNVEFLLSMKD

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=61.7788461538462, Blast_Score=533, Evalue=1e-152,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 51801; Mature: 51801

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPENKENTSVDTSLTCEDNSVDKQNPEQYHQQIVQERSQQPEIITKIADIQRMNIDQLN
CCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
QYGKKIGLKHLGSLTKSQMVFEIVKAISENPNEVLYGEGVLEILPDGFGFLRSPNYNYLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCHHHCCCCCCCCC
SAEDIYVSPAQIRRFDLKKGDTLCGTIRPPKDKEKYFALLKVDKINGRPPEKAKERILFE
CCCCEEECHHHHHHECCCCCCCEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHH
NLTPLYPNERMVMETSKEKLSTRVLDLAAPVGKGQRGLIVAPPRSGKTILLQNIANAIAC
CCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEHHHHHHHHHCC
NNPEIVLIVLLIDERPEEVTDMQRIVRGEVISSTFDEPPERHVQVAEMAIEKARRLVEHG
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
NDVVILLDSITRLARAYNTIQPHSGKILTGGIDANALHKPKRFFGAARNIEQGGSLTIIA
CCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHCCCHHHHHHHCCCCCCCCEEEEE
TALIDTGSRMDEVIFEEFKGTGNMELVLDRRLADRRIYPAIDLIKSGTRKEELLYHPNEL
EEHHCCCCHHHHHHHHHHCCCCCEEEEEECHHHCCCCHHHHHHHHCCCCCHHHEECCCHH
EKIYLLRQAVADLAPGDAMNLLLGRLKKTGSNVEFLLSMKD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MDPENKENTSVDTSLTCEDNSVDKQNPEQYHQQIVQERSQQPEIITKIADIQRMNIDQLN
CCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
QYGKKIGLKHLGSLTKSQMVFEIVKAISENPNEVLYGEGVLEILPDGFGFLRSPNYNYLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCHHHCCCCCCCCC
SAEDIYVSPAQIRRFDLKKGDTLCGTIRPPKDKEKYFALLKVDKINGRPPEKAKERILFE
CCCCEEECHHHHHHECCCCCCCEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHH
NLTPLYPNERMVMETSKEKLSTRVLDLAAPVGKGQRGLIVAPPRSGKTILLQNIANAIAC
CCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEHHHHHHHHHCC
NNPEIVLIVLLIDERPEEVTDMQRIVRGEVISSTFDEPPERHVQVAEMAIEKARRLVEHG
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
NDVVILLDSITRLARAYNTIQPHSGKILTGGIDANALHKPKRFFGAARNIEQGGSLTIIA
CCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHCCCHHHHHHHCCCCCCCCEEEEE
TALIDTGSRMDEVIFEEFKGTGNMELVLDRRLADRRIYPAIDLIKSGTRKEELLYHPNEL
EEHHCCCCHHHHHHHHHHCCCCCEEEEEECHHHCCCCHHHHHHHHCCCCCHHHEECCCHH
EKIYLLRQAVADLAPGDAMNLLLGRLKKTGSNVEFLLSMKD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]