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The map label for this gene is sigA [H]

Identifier: 46445811

GI number: 46445811

Start: 266766

End: 268430

Strand: Reverse

Name: sigA [H]

Synonym: pc0177

Alternate gene names: 46445811

Gene position: 268430-266766 (Counterclockwise)

Preceding gene: 46445814

Following gene: 46445810

Centisome position: 11.12

GC content: 38.68

Gene sequence:

>1665_bases
ATGAACAAAACGAACTTCAAAAATCATTTTTCTCAACAACATCAGCAAAAAATTGATGAGCTTGTCGCTATTTCAAAAGA
CCAAGGTTTTATTACTTATGAGGAGATCAATGAAATTCTCCCTATGTCTTTTGATTCCGCTGATCAAATTGATCAGATCC
TGATTTTCCTTAGCGGAATGGACATTCAAGTTCTTAACCAATCTGAGGTAGATCGTCAAAAAGAGCGCAAGAAAGAAGCG
AAAGAATTAGAAAGCATGCCAAAAAGATCAGAAGGATCTCCTGATGACCCGGTGCGCATGTATCTTAAAGAAATGGGTTC
GGTCCCTCTTTTAAGCCGGGAAGAAGAAGTTGAAATTTCTAAACGCATTGAAAAAGCTCAAGTCCAAATTGAACGCATTA
TTTTACGCTTTCGGTACGCAACCTCTGAAGCCATTGCTATAGCTAACTATCTTCTTACTGGTAAAGAACGATTTGATAAA
TCTATTACCGAAAAAGAAGTCGTGAATAAACAAGAATTCATGAATCTGCTTCCTAGAATATGTCAGTTATTAAAAGAAGA
AGATCTCATTTTAGAAAGATTATTAGAGCAAATTAATGATCCTAATATCAAAAAGAATGACCGCTTAAAGCTAATTGAAG
ACATTGAAAAATGTCGTATCCGCATGCAAGCCTACCTACGCCGACTGCATTTAAGACACAATATCATCGACGATTTCGTC
GAAGTTATTCTACGTGCTTATGATCGCTTCCTTTTCCTAGAAAAGGAAATTCAGGAACTTACACCTCGTGCGGAAAGAAA
TAAGTTTGCTGGTGCAAAGCTTGCCGCTGCTCAACGAAAACTCAACAAACGAGAACTTGCAGCAGGGCGATCTTTAGATG
ATTTCAAAAAAGATGTGAAAATGCTTCAACGCTGGATGGATAAAAGCCAAGAAGCTAAACGGGAAATGGTAGAATCTAAC
TTAAGGCTCGTTATTTCTATTGCTAAGAAATATACAAATCGAGGCCTTTCTTTCTTGGATCTCATTCAAGAAGGAAATAT
GGGGCTCATGAAAGCCGTCGAAAAATTTGAATATCGTCGCGGATACAAGTTTTCTACTTATGCAACATGGTGGATCAGAC
AAGCTGTCACAAGGGCTATTGCCGATCAAGCCCGTACCATCCGTATTCCTGTTCATATGATTGAAACAATTAATAAAGTC
TTACGCGGCGCAAAGAAATTAATGATGGAAACTGGTCGCGAGCCAAGTCCTGAAGAATTAGCTAAAGAACTAGGAATTAC
TGCCGAAAGGGTGAGAGAAATCTATAAAATTGCTCAACATCCTATTTCACTTCAAGCCGAAGTGGGTGATGGAGGTGAAA
GTCAATTCGGAGACTTCTTAGAAGACACGGGAGCTGATTCTCCTGCAGAAGCAACGGGTTATTCAATTTTGAAAGATAAG
ATGGGAGAAGTCCTCTCAACACTTAGTGATCGAGAACGAAAAGTCCTTATTCAACGATTTGGTTTATTAGATGGTAAACC
TAAAACACTGGAAGAAGTAGGTGTAGAGTTTAATGTTACTCGTGAACGTATCCGCCAGATTGAAGCAAAAGCTCTACGCA
AAATGCGACACCCTATCCGTTCTAAACAACTCAAAGCCTTCCTTGATTTATTAGAAGCAGAATAA

Upstream 100 bases:

>100_bases
CCAAATAAGCAAATTTTATTGAGCTTAGCGAAAACTCGCTTTAAAAAGGCAAATTTGTTAAACTTACTCGTATTTCTTAC
TTCTTCTACTTTAGGAAAGC

Downstream 100 bases:

>100_bases
ATTAATATCCTCTCTTAATAAAAGAGGGGATATTTGTCTTGTAACCTTAGGCTCCATTTTTAATATGTCTCATTCTCAAT
TACAAAGCCGAGGTCTTCAA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A; Sigma-66; Sigma-70 [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MNKTNFKNHFSQQHQQKIDELVAISKDQGFITYEEINEILPMSFDSADQIDQILIFLSGMDIQVLNQSEVDRQKERKKEA
KELESMPKRSEGSPDDPVRMYLKEMGSVPLLSREEEVEISKRIEKAQVQIERIILRFRYATSEAIAIANYLLTGKERFDK
SITEKEVVNKQEFMNLLPRICQLLKEEDLILERLLEQINDPNIKKNDRLKLIEDIEKCRIRMQAYLRRLHLRHNIIDDFV
EVILRAYDRFLFLEKEIQELTPRAERNKFAGAKLAAAQRKLNKRELAAGRSLDDFKKDVKMLQRWMDKSQEAKREMVESN
LRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYRRGYKFSTYATWWIRQAVTRAIADQARTIRIPVHMIETINKV
LRGAKKLMMETGREPSPEELAKELGITAERVREIYKIAQHPISLQAEVGDGGESQFGDFLEDTGADSPAEATGYSILKDK
MGEVLSTLSDRERKVLIQRFGLLDGKPKTLEEVGVEFNVTRERIRQIEAKALRKMRHPIRSKQLKAFLDLLEAE

Sequences:

>Translated_554_residues
MNKTNFKNHFSQQHQQKIDELVAISKDQGFITYEEINEILPMSFDSADQIDQILIFLSGMDIQVLNQSEVDRQKERKKEA
KELESMPKRSEGSPDDPVRMYLKEMGSVPLLSREEEVEISKRIEKAQVQIERIILRFRYATSEAIAIANYLLTGKERFDK
SITEKEVVNKQEFMNLLPRICQLLKEEDLILERLLEQINDPNIKKNDRLKLIEDIEKCRIRMQAYLRRLHLRHNIIDDFV
EVILRAYDRFLFLEKEIQELTPRAERNKFAGAKLAAAQRKLNKRELAAGRSLDDFKKDVKMLQRWMDKSQEAKREMVESN
LRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYRRGYKFSTYATWWIRQAVTRAIADQARTIRIPVHMIETINKV
LRGAKKLMMETGREPSPEELAKELGITAERVREIYKIAQHPISLQAEVGDGGESQFGDFLEDTGADSPAEATGYSILKDK
MGEVLSTLSDRERKVLIQRFGLLDGKPKTLEEVGVEFNVTRERIRQIEAKALRKMRHPIRSKQLKAFLDLLEAE
>Mature_554_residues
MNKTNFKNHFSQQHQQKIDELVAISKDQGFITYEEINEILPMSFDSADQIDQILIFLSGMDIQVLNQSEVDRQKERKKEA
KELESMPKRSEGSPDDPVRMYLKEMGSVPLLSREEEVEISKRIEKAQVQIERIILRFRYATSEAIAIANYLLTGKERFDK
SITEKEVVNKQEFMNLLPRICQLLKEEDLILERLLEQINDPNIKKNDRLKLIEDIEKCRIRMQAYLRRLHLRHNIIDDFV
EVILRAYDRFLFLEKEIQELTPRAERNKFAGAKLAAAQRKLNKRELAAGRSLDDFKKDVKMLQRWMDKSQEAKREMVESN
LRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYRRGYKFSTYATWWIRQAVTRAIADQARTIRIPVHMIETINKV
LRGAKKLMMETGREPSPEELAKELGITAERVREIYKIAQHPISLQAEVGDGGESQFGDFLEDTGADSPAEATGYSILKDK
MGEVLSTLSDRERKVLIQRFGLLDGKPKTLEEVGVEFNVTRERIRQIEAKALRKMRHPIRSKQLKAFLDLLEAE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=288, Percent_Identity=56.25, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1789098, Length=302, Percent_Identity=39.4039735099338, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1789871, Length=249, Percent_Identity=27.3092369477912, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=27.2277227722772, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 64521; Mature: 64521

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTNFKNHFSQQHQQKIDELVAISKDQGFITYEEINEILPMSFDSADQIDQILIFLSGM
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHCCC
DIQVLNQSEVDRQKERKKEAKELESMPKRSEGSPDDPVRMYLKEMGSVPLLSREEEVEIS
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
KRIEKAQVQIERIILRFRYATSEAIAIANYLLTGKERFDKSITEKEVVNKQEFMNLLPRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
CQLLKEEDLILERLLEQINDPNIKKNDRLKLIEDIEKCRIRMQAYLRRLHLRHNIIDDFV
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVILRAYDRFLFLEKEIQELTPRAERNKFAGAKLAAAQRKLNKRELAAGRSLDDFKKDVK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MLQRWMDKSQEAKREMVESNLRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHC
GYKFSTYATWWIRQAVTRAIADQARTIRIPVHMIETINKVLRGAKKLMMETGREPSPEEL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AKELGITAERVREIYKIAQHPISLQAEVGDGGESQFGDFLEDTGADSPAEATGYSILKDK
HHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
MGEVLSTLSDRERKVLIQRFGLLDGKPKTLEEVGVEFNVTRERIRQIEAKALRKMRHPIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SKQLKAFLDLLEAE
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNKTNFKNHFSQQHQQKIDELVAISKDQGFITYEEINEILPMSFDSADQIDQILIFLSGM
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHCCC
DIQVLNQSEVDRQKERKKEAKELESMPKRSEGSPDDPVRMYLKEMGSVPLLSREEEVEIS
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHH
KRIEKAQVQIERIILRFRYATSEAIAIANYLLTGKERFDKSITEKEVVNKQEFMNLLPRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
CQLLKEEDLILERLLEQINDPNIKKNDRLKLIEDIEKCRIRMQAYLRRLHLRHNIIDDFV
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVILRAYDRFLFLEKEIQELTPRAERNKFAGAKLAAAQRKLNKRELAAGRSLDDFKKDVK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
MLQRWMDKSQEAKREMVESNLRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHC
GYKFSTYATWWIRQAVTRAIADQARTIRIPVHMIETINKVLRGAKKLMMETGREPSPEEL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AKELGITAERVREIYKIAQHPISLQAEVGDGGESQFGDFLEDTGADSPAEATGYSILKDK
HHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
MGEVLSTLSDRERKVLIQRFGLLDGKPKTLEEVGVEFNVTRERIRQIEAKALRKMRHPIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SKQLKAFLDLLEAE
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]