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The map label for this gene is eno

Identifier: 46445777

GI number: 46445777

Start: 217864

End: 219168

Strand: Direct

Name: eno

Synonym: pc0143

Alternate gene names: 46445777

Gene position: 217864-219168 (Clockwise)

Preceding gene: 46445776

Following gene: 46445778

Centisome position: 9.02

GC content: 37.7

Gene sequence:

>1305_bases
ATGTCATATATAAGATCTGTAAAAGGAAGAGAAATTTTAGATTCTAGAGGTAATCCAACTGTTGAGGTTGAACTAATTAC
AGATCAAAATGTCATGGTTAAAGCGTCTGTTCCTTCTGGAGCTTCTACAGGAGAAAATGAGGCATTAGAATTACGTGATG
GGGATCTCAATTATTACTTTGGTAAAGGAGTTTTAAAAGCAATTGCTAATATTAATGGTCCAATTGCACAAATTCTGGTT
GGAGAGCAGGTCTATGATCAAGAAAGATTAGATCGGATGTTAATTGAAGCTGATGGGACAGACAATAAAGGTCATTTCGG
AGCCAACGCGATTTTAGGTGTTTCGCTAGCTTTAGCACGGGCGGGAGCGTTGACAGCAAATCTCCCTTTATATCGCTACA
TTGGAGGGGTTCATGCCCATCTTTTACCATGTCCCATGATGAATATTATTAATGGAGGGGCTCATGCTGATAACTCTCTT
GATTTTCAAGAATTTATGATTCGACCTGTTGGAGCTTCTACTCTTCGTGAAGCGGTTCGGTGGGGAGCTGAAATTTTTCA
CACGTTAAAAGGAATACTTAAAAAAGCTGGGCATGTGACAGCTGTTGGAGATGAAGGGGGTTTTGCACCTAACCTTTCCT
CAAATGAAGAGGCTTTAGAATTGATTTTATTAGCGATTGAAAAAGCTGGTTATCAACCTGGTAAACAAGTGGTTCTAGCA
ATGGATTGTGCAGCTTCGGAATTTTATGATAAAACTACTAAGCAATATTTTGAAAAGAAAAAGAAACAAAAGAATCAAGT
ATTTGCGGAAAGAACCGCTGAAGAGCAAATTGCCTATCTTGAAACCCTTTGTCAAAATTATCCAATTGATTCAATTGAAG
ATGCGTTAGCTGAAGGAGATTGGTGTGGTTGGCAACGATTAACTGAAAGATTAGGACGAAAAATACAAATTGTAGGAGAT
GATATTTTTGTCACCAACCCCAAATTTCTGGAAAAAGGAATTGAACTTGGTGTGGGGAATGCAATTTTAGTGAAAATTAA
TCAAATCGGAACACTAACCGAAACGCTGCAAACAATTCGTTTGGCTCAAACTAACAATTATGCTTGTATTATTTCTCATC
GATCTGGCGAAACAGAAGATAGTATCATTGCCGATATTAGTGTAGCGGCTAATACAGGACAAATCAAAACAGGTTCTCTT
TCTAGATCTGACCGAATTGCTAAGTACAATCGGTTATTAGCTATTGAGGCAGAATTAGAATCGACAGCGCTTTATCAAGA
TAGTAATCGATTTCAGATTTTCTAA

Upstream 100 bases:

>100_bases
GTTGAAGAAGAGCATATTTCTTTTGTTCGTTATATGTCTTATCAAGTATTAATGGAAACTAGTAATGAGAAGCATATTAG
AAGATAACTGAAGAGGAATT

Downstream 100 bases:

>100_bases
GGTAAATTGATGGATACAATTTATACAATCGTTTTATTTGGAGAGGCTGAACGCGGAGAGTTTCGCACGGCTTATCTTTG
TCAAGGATTAGAAGATTTAG

Product: phosphopyruvate hydratase (enolase)

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSYIRSVKGREILDSRGNPTVEVELITDQNVMVKASVPSGASTGENEALELRDGDLNYYFGKGVLKAIANINGPIAQILV
GEQVYDQERLDRMLIEADGTDNKGHFGANAILGVSLALARAGALTANLPLYRYIGGVHAHLLPCPMMNIINGGAHADNSL
DFQEFMIRPVGASTLREAVRWGAEIFHTLKGILKKAGHVTAVGDEGGFAPNLSSNEEALELILLAIEKAGYQPGKQVVLA
MDCAASEFYDKTTKQYFEKKKKQKNQVFAERTAEEQIAYLETLCQNYPIDSIEDALAEGDWCGWQRLTERLGRKIQIVGD
DIFVTNPKFLEKGIELGVGNAILVKINQIGTLTETLQTIRLAQTNNYACIISHRSGETEDSIIADISVAANTGQIKTGSL
SRSDRIAKYNRLLAIEAELESTALYQDSNRFQIF

Sequences:

>Translated_434_residues
MSYIRSVKGREILDSRGNPTVEVELITDQNVMVKASVPSGASTGENEALELRDGDLNYYFGKGVLKAIANINGPIAQILV
GEQVYDQERLDRMLIEADGTDNKGHFGANAILGVSLALARAGALTANLPLYRYIGGVHAHLLPCPMMNIINGGAHADNSL
DFQEFMIRPVGASTLREAVRWGAEIFHTLKGILKKAGHVTAVGDEGGFAPNLSSNEEALELILLAIEKAGYQPGKQVVLA
MDCAASEFYDKTTKQYFEKKKKQKNQVFAERTAEEQIAYLETLCQNYPIDSIEDALAEGDWCGWQRLTERLGRKIQIVGD
DIFVTNPKFLEKGIELGVGNAILVKINQIGTLTETLQTIRLAQTNNYACIISHRSGETEDSIIADISVAANTGQIKTGSL
SRSDRIAKYNRLLAIEAELESTALYQDSNRFQIF
>Mature_433_residues
SYIRSVKGREILDSRGNPTVEVELITDQNVMVKASVPSGASTGENEALELRDGDLNYYFGKGVLKAIANINGPIAQILVG
EQVYDQERLDRMLIEADGTDNKGHFGANAILGVSLALARAGALTANLPLYRYIGGVHAHLLPCPMMNIINGGAHADNSLD
FQEFMIRPVGASTLREAVRWGAEIFHTLKGILKKAGHVTAVGDEGGFAPNLSSNEEALELILLAIEKAGYQPGKQVVLAM
DCAASEFYDKTTKQYFEKKKKQKNQVFAERTAEEQIAYLETLCQNYPIDSIEDALAEGDWCGWQRLTERLGRKIQIVGDD
IFVTNPKFLEKGIELGVGNAILVKINQIGTLTETLQTIRLAQTNNYACIISHRSGETEDSIIADISVAANTGQIKTGSLS
RSDRIAKYNRLLAIEAELESTALYQDSNRFQIF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=51.9630484988453, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=50.6912442396313, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=51.1627906976744, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=51.1627906976744, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=46.4953271028037, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI169201331, Length=359, Percent_Identity=25.6267409470752, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI169201757, Length=359, Percent_Identity=25.6267409470752, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239744207, Length=359, Percent_Identity=25.6267409470752, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=56.4102564102564, Blast_Score=467, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=49.3087557603687, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=49.3087557603687, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32563855, Length=302, Percent_Identity=37.4172185430464, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321693, Length=436, Percent_Identity=47.0183486238532, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324974, Length=439, Percent_Identity=44.874715261959, Blast_Score=343, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324969, Length=439, Percent_Identity=44.874715261959, Blast_Score=343, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6323985, Length=439, Percent_Identity=44.874715261959, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6321968, Length=436, Percent_Identity=46.3302752293578, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24580918, Length=424, Percent_Identity=49.5283018867925, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580916, Length=424, Percent_Identity=49.5283018867925, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580920, Length=424, Percent_Identity=49.5283018867925, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580914, Length=424, Percent_Identity=49.5283018867925, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281360527, Length=424, Percent_Identity=49.0566037735849, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17137654, Length=424, Percent_Identity=49.0566037735849, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PARUW (Q6MEY2)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007142.1
- ProteinModelPortal:   Q6MEY2
- SMR:   Q6MEY2
- STRING:   Q6MEY2
- GeneID:   2779981
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0143
- NMPDR:   fig|264201.1.peg.143
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   CLSK2762080
- BioCyc:   CPRO264201:PC0143-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47558; Mature: 47426

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 345-345 BINDING 155-155 BINDING 164-164 BINDING 293-293 BINDING 320-320 BINDING 345-345 BINDING 396-396

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYIRSVKGREILDSRGNPTVEVELITDQNVMVKASVPSGASTGENEALELRDGDLNYYF
CCHHHHCCCCHHHHCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCEEEE
GKGVLKAIANINGPIAQILVGEQVYDQERLDRMLIEADGTDNKGHFGANAILGVSLALAR
HHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHH
AGALTANLPLYRYIGGVHAHLLPCPMMNIINGGAHADNSLDFQEFMIRPVGASTLREAVR
CCCCCCCCCHHHHHCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
WGAEIFHTLKGILKKAGHVTAVGDEGGFAPNLSSNEEALELILLAIEKAGYQPGKQVVLA
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
MDCAASEFYDKTTKQYFEKKKKQKNQVFAERTAEEQIAYLETLCQNYPIDSIEDALAEGD
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
WCGWQRLTERLGRKIQIVGDDIFVTNPKFLEKGIELGVGNAILVKINQIGTLTETLQTIR
CCCHHHHHHHHCCEEEEEECCEEECCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
LAQTNNYACIISHRSGETEDSIIADISVAANTGQIKTGSLSRSDRIAKYNRLLAIEAELE
HEECCCEEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHHHHEEEEEEECCC
STALYQDSNRFQIF
HHHEEECCCCEEEC
>Mature Secondary Structure 
SYIRSVKGREILDSRGNPTVEVELITDQNVMVKASVPSGASTGENEALELRDGDLNYYF
CHHHHCCCCHHHHCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCEEEEECCCCEEEE
GKGVLKAIANINGPIAQILVGEQVYDQERLDRMLIEADGTDNKGHFGANAILGVSLALAR
HHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHH
AGALTANLPLYRYIGGVHAHLLPCPMMNIINGGAHADNSLDFQEFMIRPVGASTLREAVR
CCCCCCCCCHHHHHCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCHHHHHHHHH
WGAEIFHTLKGILKKAGHVTAVGDEGGFAPNLSSNEEALELILLAIEKAGYQPGKQVVLA
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
MDCAASEFYDKTTKQYFEKKKKQKNQVFAERTAEEQIAYLETLCQNYPIDSIEDALAEGD
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
WCGWQRLTERLGRKIQIVGDDIFVTNPKFLEKGIELGVGNAILVKINQIGTLTETLQTIR
CCCHHHHHHHHCCEEEEEECCEEECCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHH
LAQTNNYACIISHRSGETEDSIIADISVAANTGQIKTGSLSRSDRIAKYNRLLAIEAELE
HEECCCEEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHHHHEEEEEEECCC
STALYQDSNRFQIF
HHHEEECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA