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The map label for this gene is greA [H]

Identifier: 46445720

GI number: 46445720

Start: 145079

End: 147250

Strand: Reverse

Name: greA [H]

Synonym: pc0086

Alternate gene names: 46445720

Gene position: 147250-145079 (Counterclockwise)

Preceding gene: 46445725

Following gene: 46445719

Centisome position: 6.1

GC content: 34.58

Gene sequence:

>2172_bases
ATGGGGTATTTGAAGGAGTTTTTGACGCAAATCAATAATCGGGATTTTCATAAATTTCTCGTTTTATGGGAGGAATATTG
CACAAGTGATACGGTAGATTTCGAAGAATTTAGCCAACTTTTAAAAGCAATTAAATCATCCGATTTAAGCAAACACTTTG
GACAAATAATCGAAACAGCCCTCCCTCTTTGGAAAACAATAACTGACCCTGAGCAATCTTACGAAATTTTGCGATTACTC
ATTGACATACAAACTACCAACCATCCCACTTTAATAGATTTAACATTAGAAACCCTTCAAAAATATCATGGTAATCATCC
TAAATTTCAAGAGAGATTAAAACTTGCTGGATTAAGAGACAAAGATCAATTCCAGGGGGCCATTTCTAAATATGATTTAA
TTAGTCACTTAGCAAAAGGTAATATGGTTTTCCATAATGCTGGCTGGGGAACAGGAGAAATCATGGATGTCTCTTTTGTT
CGGGAGCATTTGGTCATTGAATTCGAAAATATTTCAGGACGTAAAGACCTCTCTTTTGCTAATGCACTTAAAACCCTAGT
TCCTTTATCCCCTAAACATTTTTTAGCAAGACGTTTTGCTGACCCCGACAGTTTAGAAAAAGAAGGACGTGATAATCCTG
TTAAACTTATTAAACTTCTTCTTCATGATATTGGGCCAAAGACGGCAGCAGAAATAAAAGATGAACTTTGCGTACTTGTT
ATCCCAGAAAAAGATTGGACAAAATGGTGGCAAGGAGCCCGTGCAAAAATTAAAAAAGACCCTATGATCGAAACCCCTGA
TCATTTAAGAGAACCTTTCTTGCTAAGAAAAGCTGAGTTATCCACGGAAGAACGTCTTAAAAATGCTATGCACAATAAAA
CAGAAGTAGATCAAATTATTCAAACTACCTATACTTTTGTGCGTGATACAGCCAATGCTTTAAAAAATACAGACACAAAA
AAATCTTTGCAAACAAAACTAACAGATCTTTTAGAGCTTCCCGATCTAAGTGAAGTACAATTGTTACAAATTCATTTATT
GCTTGAACAGTTTTTTGTTAATAAATCATCTACAGAAGAAATTTGTAAATTGATACAAAGCACTAAAAAAATTGAATCCC
TTATCCAAGATATTGACATTGTTGCCTTTAAAAAACGTGCATTAGTTTATGTTAAAGAACAAAGATCGGATTGGCCCTCA
CTTTTCTTAACTTTACTTTTCGAATTACCCCAAGCTCAATTACGAGATTATTTATTAAAAGAATTAAATCAAGGTCTCAC
TACTAAACCAGCCTTAGAATCTAAACTCCAAGAATTGATTGATAATCCGAGCCTATCGCCAGAATTGTTTGTTTGGTATT
TCCAGAAACTTCTAAGCAAAGAAGAAAATTTACCCTTTCAATCAAAGTTAGGGCTTGGCCAATTTTTTGAATCTTTCTTT
ATTCTGTTTAGTGCTCTAGAAAACCGACCTGAATATAGAGATCTTTCTAAAAAAATGTATTCCATGTTATCAGGGAAACG
ATACGCGCTTGTACGGGAACTTTTACAAGGTACAAGCTTAGAATATACGAAAGAGATTTTACTTTTAGCTTCTAAATGTC
AGTCTTTAACAGATCATGATATGACAATTTTACGCTCTTTAGCAGGTGTTGTTCATCCTTCCCTCGCTTCTTCCAAACCA
CGCAAAGGACAAGCAAAAACAGATGATGAAGAAATTTGGACTACAGAAGAGGGATACTTAAAAATTCAAGATAGAATTCG
TCAAATCGGAACAGTTGAAGTTGTAGAAAATGCGCGAGAAATTGAAGCCGCTCGAGCTCTGGGAGATTTACGCGAAAACT
CTGAATTTAAATTTGCTCAAGAACGCCGCGCTAGATTACAATCAGAGTTAAAAACACTATCGGAACAATTCGGACGTGCT
CGAATTATCACTCCTGATGATATTCTTACTGATGAAGTAGGAATTGGTAGTGTCATTGATTTAATTGATAGTCAAGGACA
AAAAATTAGATATTCAATTTTAGGGCCTTGGGACGCAGATCCGGAAAAAAATATACTTTCTCTCAATTCAAAATTTGCAC
AAGCAATGATAGGAAAAAAAATTGGGGATCAGTTTCAATTTAAAGATGAAACTTTCAAAGTTTTAAGCTTACATAGTTAT
TTAAGGTCTTAA

Upstream 100 bases:

>100_bases
GAATGAGAAAGCGTGTGTGTTTGATTAATATTCTCCACTCAACATCAACCATGACATCTGAAGATTCTCTTAAAATGGAG
ACATAACTTAAGGTTCGGTA

Downstream 100 bases:

>100_bases
TCTATAGTTTATCTTTTCAGTTCAGAAATTTGCAAAGATGTTATCCATTTTTGCAAATTCCTCGAATTTCTTGAATAAAT
CCGCCTTTGCTTAACCAAGA

Product: transcript cleavage factor/unknown domain fusion protein

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MGYLKEFLTQINNRDFHKFLVLWEEYCTSDTVDFEEFSQLLKAIKSSDLSKHFGQIIETALPLWKTITDPEQSYEILRLL
IDIQTTNHPTLIDLTLETLQKYHGNHPKFQERLKLAGLRDKDQFQGAISKYDLISHLAKGNMVFHNAGWGTGEIMDVSFV
REHLVIEFENISGRKDLSFANALKTLVPLSPKHFLARRFADPDSLEKEGRDNPVKLIKLLLHDIGPKTAAEIKDELCVLV
IPEKDWTKWWQGARAKIKKDPMIETPDHLREPFLLRKAELSTEERLKNAMHNKTEVDQIIQTTYTFVRDTANALKNTDTK
KSLQTKLTDLLELPDLSEVQLLQIHLLLEQFFVNKSSTEEICKLIQSTKKIESLIQDIDIVAFKKRALVYVKEQRSDWPS
LFLTLLFELPQAQLRDYLLKELNQGLTTKPALESKLQELIDNPSLSPELFVWYFQKLLSKEENLPFQSKLGLGQFFESFF
ILFSALENRPEYRDLSKKMYSMLSGKRYALVRELLQGTSLEYTKEILLLASKCQSLTDHDMTILRSLAGVVHPSLASSKP
RKGQAKTDDEEIWTTEEGYLKIQDRIRQIGTVEVVENAREIEAARALGDLRENSEFKFAQERRARLQSELKTLSEQFGRA
RIITPDDILTDEVGIGSVIDLIDSQGQKIRYSILGPWDADPEKNILSLNSKFAQAMIGKKIGDQFQFKDETFKVLSLHSY
LRS

Sequences:

>Translated_723_residues
MGYLKEFLTQINNRDFHKFLVLWEEYCTSDTVDFEEFSQLLKAIKSSDLSKHFGQIIETALPLWKTITDPEQSYEILRLL
IDIQTTNHPTLIDLTLETLQKYHGNHPKFQERLKLAGLRDKDQFQGAISKYDLISHLAKGNMVFHNAGWGTGEIMDVSFV
REHLVIEFENISGRKDLSFANALKTLVPLSPKHFLARRFADPDSLEKEGRDNPVKLIKLLLHDIGPKTAAEIKDELCVLV
IPEKDWTKWWQGARAKIKKDPMIETPDHLREPFLLRKAELSTEERLKNAMHNKTEVDQIIQTTYTFVRDTANALKNTDTK
KSLQTKLTDLLELPDLSEVQLLQIHLLLEQFFVNKSSTEEICKLIQSTKKIESLIQDIDIVAFKKRALVYVKEQRSDWPS
LFLTLLFELPQAQLRDYLLKELNQGLTTKPALESKLQELIDNPSLSPELFVWYFQKLLSKEENLPFQSKLGLGQFFESFF
ILFSALENRPEYRDLSKKMYSMLSGKRYALVRELLQGTSLEYTKEILLLASKCQSLTDHDMTILRSLAGVVHPSLASSKP
RKGQAKTDDEEIWTTEEGYLKIQDRIRQIGTVEVVENAREIEAARALGDLRENSEFKFAQERRARLQSELKTLSEQFGRA
RIITPDDILTDEVGIGSVIDLIDSQGQKIRYSILGPWDADPEKNILSLNSKFAQAMIGKKIGDQFQFKDETFKVLSLHSY
LRS
>Mature_722_residues
GYLKEFLTQINNRDFHKFLVLWEEYCTSDTVDFEEFSQLLKAIKSSDLSKHFGQIIETALPLWKTITDPEQSYEILRLLI
DIQTTNHPTLIDLTLETLQKYHGNHPKFQERLKLAGLRDKDQFQGAISKYDLISHLAKGNMVFHNAGWGTGEIMDVSFVR
EHLVIEFENISGRKDLSFANALKTLVPLSPKHFLARRFADPDSLEKEGRDNPVKLIKLLLHDIGPKTAAEIKDELCVLVI
PEKDWTKWWQGARAKIKKDPMIETPDHLREPFLLRKAELSTEERLKNAMHNKTEVDQIIQTTYTFVRDTANALKNTDTKK
SLQTKLTDLLELPDLSEVQLLQIHLLLEQFFVNKSSTEEICKLIQSTKKIESLIQDIDIVAFKKRALVYVKEQRSDWPSL
FLTLLFELPQAQLRDYLLKELNQGLTTKPALESKLQELIDNPSLSPELFVWYFQKLLSKEENLPFQSKLGLGQFFESFFI
LFSALENRPEYRDLSKKMYSMLSGKRYALVRELLQGTSLEYTKEILLLASKCQSLTDHDMTILRSLAGVVHPSLASSKPR
KGQAKTDDEEIWTTEEGYLKIQDRIRQIGTVEVVENAREIEAARALGDLRENSEFKFAQERRARLQSELKTLSEQFGRAR
IITPDDILTDEVGIGSVIDLIDSQGQKIRYSILGPWDADPEKNILSLNSKFAQAMIGKKIGDQFQFKDETFKVLSLHSYL
RS

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG1747

COG function: function code S; Uncharacterized N-terminal domain of the transcription elongation factor GreA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GRAD1 (greA associated 1) domain [H]

Homologues:

None

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 83313; Mature: 83181

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00830 GREAB_2 ; PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYLKEFLTQINNRDFHKFLVLWEEYCTSDTVDFEEFSQLLKAIKSSDLSKHFGQIIETA
CCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
LPLWKTITDPEQSYEILRLLIDIQTTNHPTLIDLTLETLQKYHGNHPKFQERLKLAGLRD
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHCCCCC
KDQFQGAISKYDLISHLAKGNMVFHNAGWGTGEIMDVSFVREHLVIEFENISGRKDLSFA
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHEEEECCCCCCCCHHHH
NALKTLVPLSPKHFLARRFADPDSLEKEGRDNPVKLIKLLLHDIGPKTAAEIKDELCVLV
HHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHCCCEEEEE
IPEKDWTKWWQGARAKIKKDPMIETPDHLREPFLLRKAELSTEERLKNAMHNKTEVDQII
ECCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHCCHHHHHHHHHCCHHHHHHHH
QTTYTFVRDTANALKNTDTKKSLQTKLTDLLELPDLSEVQLLQIHLLLEQFFVNKSSTEE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHH
ICKLIQSTKKIESLIQDIDIVAFKKRALVYVKEQRSDWPSLFLTLLFELPQAQLRDYLLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHH
ELNQGLTTKPALESKLQELIDNPSLSPELFVWYFQKLLSKEENLPFQSKLGLGQFFESFF
HHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHH
ILFSALENRPEYRDLSKKMYSMLSGKRYALVRELLQGTSLEYTKEILLLASKCQSLTDHD
HHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHH
MTILRSLAGVVHPSLASSKPRKGQAKTDDEEIWTTEEGYLKIQDRIRQIGTVEVVENARE
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IEAARALGDLRENSEFKFAQERRARLQSELKTLSEQFGRARIITPDDILTDEVGIGSVID
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHCCCCCCHHHHH
LIDSQGQKIRYSILGPWDADPEKNILSLNSKFAQAMIGKKIGDQFQFKDETFKVLSLHSY
HHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LRS
HCC
>Mature Secondary Structure 
GYLKEFLTQINNRDFHKFLVLWEEYCTSDTVDFEEFSQLLKAIKSSDLSKHFGQIIETA
CHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
LPLWKTITDPEQSYEILRLLIDIQTTNHPTLIDLTLETLQKYHGNHPKFQERLKLAGLRD
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHCCCCC
KDQFQGAISKYDLISHLAKGNMVFHNAGWGTGEIMDVSFVREHLVIEFENISGRKDLSFA
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHEEEECCCCCCCCHHHH
NALKTLVPLSPKHFLARRFADPDSLEKEGRDNPVKLIKLLLHDIGPKTAAEIKDELCVLV
HHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHCCCEEEEE
IPEKDWTKWWQGARAKIKKDPMIETPDHLREPFLLRKAELSTEERLKNAMHNKTEVDQII
ECCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHCCHHHHHHHHHCCHHHHHHHH
QTTYTFVRDTANALKNTDTKKSLQTKLTDLLELPDLSEVQLLQIHLLLEQFFVNKSSTEE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHH
ICKLIQSTKKIESLIQDIDIVAFKKRALVYVKEQRSDWPSLFLTLLFELPQAQLRDYLLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHH
ELNQGLTTKPALESKLQELIDNPSLSPELFVWYFQKLLSKEENLPFQSKLGLGQFFESFF
HHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHH
ILFSALENRPEYRDLSKKMYSMLSGKRYALVRELLQGTSLEYTKEILLLASKCQSLTDHD
HHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHH
MTILRSLAGVVHPSLASSKPRKGQAKTDDEEIWTTEEGYLKIQDRIRQIGTVEVVENARE
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IEAARALGDLRENSEFKFAQERRARLQSELKTLSEQFGRARIITPDDILTDEVGIGSVID
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHCCCCCCHHHHH
LIDSQGQKIRYSILGPWDADPEKNILSLNSKFAQAMIGKKIGDQFQFKDETFKVLSLHSY
HHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LRS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]