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The map label for this gene is argG

Identifier: 45659143

GI number: 45659143

Start: 4079853

End: 4081064

Strand: Direct

Name: argG

Synonym: LIC13324

Alternate gene names: 45659143

Gene position: 4079853-4081064 (Clockwise)

Preceding gene: 45659138

Following gene: 45659144

Centisome position: 95.39

GC content: 39.52

Gene sequence:

>1212_bases
ATGGCGCAAAACAAACCTGTTAAGAAAATCGTTCTCGCTTATTCCGGTGGATTGGATACGTCCGTAATTCTTACCTGGTT
GAAAGATACATACGGTTGCGAAGTAATCGCTTTTACTGCGGATATAGGTCAAAAGGAAGAACTTTCCGGACTAGAAGAAA
AAGGAATTAAAACAGGAGCTTCTAAGGTTTATATCCAAGATTTGCGTTTAGAGTTTGCTAGAGATTTTATTTTCCCAGCC
ATTCAAGGAAATGCAATTTATGAAATGAGGTATCTTCTTGGAACCTCTTTGGCTCGGCCTTTAATTGCCAAGGCAATGGT
AGAAGTGGCCGAAAAAGAAGGTGCAGACGCATTTGCACACGGGGCCACTGGCAAAGGAAATGACCAAGTTCGCTTTGAAT
TAGGAGTCAAATCCTTAGCTCCTGAAAAAACAATCATCGCTCCTTGGAGAATCTGGAGTTTTGGCGGTAGATCAGATCTA
ATTGAATACGCAAAATCCAAAGGAATTCCAGTTCCAGTAACTGCGGAAAAACCGTATTCGATGGACCGCAATCTAATGCA
TATATCTTATGAAGGTGGAATATTAGAAGATCCTTATAAAGAACCAGATGAGAAAATGTTTTTATTAACAACCTCTCCCG
AAAAAGCTCCCGATGCTCCTGAATATTTGGAACTCGATTTCGAAGAAGGAAATTGTGTAGCAATCAACGGTAAGAAAATG
AATCCTCTTGAAATCATGGAAACGCTCAATACGATTGCGGGCAAACATGGAGTGGGAAGAGTTGACATCGTTGAAAACCG
ACTCGTAGGAATCAAATCTAGAGGAGTTTACGAAACTCCCGGCGGGACTATATTATTTTTAGCTCATAGAGACTTAGAAT
CGATTACGATCGATCGAGACACACAACATCATAAAGATAAACTTTCCATCGAATTTGCAGAACTCATTTATAACGGACAT
TGGTTTTCTTCCAGAATGAAAGCAGTTCGAGCTTTTATTACAGAAACACAAAGATATGTTAGTGGAACCGTAAGAATAAA
ACTTTACAAAGGGATTTGTTCTGTTGTAGGAAGAAAATCCCAAGTATCTCTTTACAACCCAGAAATGGCGACTTTCGAAA
AAGAAGAATTATACAATCAGAAAGACGCCGAAGGTTTTATAAACATCTACGGCTTACCCGCTCAAGAAACGGCAAGGCTT
CGCAAAAAATGA

Upstream 100 bases:

>100_bases
TTCCGTAGCGGAGAGCCAAAACTAAACACTCCAAATCCCGTAGTAAAATCGGGAAAATCCGGTTGTCTAGAGACCCGTAC
CAATTTTCAGTGTTCTCAGA

Downstream 100 bases:

>100_bases
AACACCTGGCAATTTATCCAGGCTCCTTTGATCCTCTTACAAAAGGTCATTTGGATATTCTTCAGAGATCTTTGGGACTT
TTCGACAAAGTAATCATTGC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MAQNKPVKKIVLAYSGGLDTSVILTWLKDTYGCEVIAFTADIGQKEELSGLEEKGIKTGASKVYIQDLRLEFARDFIFPA
IQGNAIYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAHGATGKGNDQVRFELGVKSLAPEKTIIAPWRIWSFGGRSDL
IEYAKSKGIPVPVTAEKPYSMDRNLMHISYEGGILEDPYKEPDEKMFLLTTSPEKAPDAPEYLELDFEEGNCVAINGKKM
NPLEIMETLNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTILFLAHRDLESITIDRDTQHHKDKLSIEFAELIYNGH
WFSSRMKAVRAFITETQRYVSGTVRIKLYKGICSVVGRKSQVSLYNPEMATFEKEELYNQKDAEGFINIYGLPAQETARL
RKK

Sequences:

>Translated_403_residues
MAQNKPVKKIVLAYSGGLDTSVILTWLKDTYGCEVIAFTADIGQKEELSGLEEKGIKTGASKVYIQDLRLEFARDFIFPA
IQGNAIYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAHGATGKGNDQVRFELGVKSLAPEKTIIAPWRIWSFGGRSDL
IEYAKSKGIPVPVTAEKPYSMDRNLMHISYEGGILEDPYKEPDEKMFLLTTSPEKAPDAPEYLELDFEEGNCVAINGKKM
NPLEIMETLNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTILFLAHRDLESITIDRDTQHHKDKLSIEFAELIYNGH
WFSSRMKAVRAFITETQRYVSGTVRIKLYKGICSVVGRKSQVSLYNPEMATFEKEELYNQKDAEGFINIYGLPAQETARL
RKK
>Mature_402_residues
AQNKPVKKIVLAYSGGLDTSVILTWLKDTYGCEVIAFTADIGQKEELSGLEEKGIKTGASKVYIQDLRLEFARDFIFPAI
QGNAIYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAHGATGKGNDQVRFELGVKSLAPEKTIIAPWRIWSFGGRSDLI
EYAKSKGIPVPVTAEKPYSMDRNLMHISYEGGILEDPYKEPDEKMFLLTTSPEKAPDAPEYLELDFEEGNCVAINGKKMN
PLEIMETLNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTILFLAHRDLESITIDRDTQHHKDKLSIEFAELIYNGHW
FSSRMKAVRAFITETQRYVSGTVRIKLYKGICSVVGRKSQVSLYNPEMATFEKEELYNQKDAEGFINIYGLPAQETARLR
KK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=403, Percent_Identity=52.1091811414392, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI16950633, Length=403, Percent_Identity=52.1091811414392, Blast_Score=407, Evalue=1e-113,
Organism=Escherichia coli, GI1789563, Length=415, Percent_Identity=30.6024096385542, Blast_Score=159, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324514, Length=399, Percent_Identity=45.3634085213033, Blast_Score=347, Evalue=2e-96,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=46.4373464373464, Blast_Score=357, Evalue=1e-98,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 45075; Mature: 44944

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQNKPVKKIVLAYSGGLDTSVILTWLKDTYGCEVIAFTADIGQKEELSGLEEKGIKTGA
CCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCHHHCCCCCC
SKVYIQDLRLEFARDFIFPAIQGNAIYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAH
CEEEHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GATGKGNDQVRFELGVKSLAPEKTIIAPWRIWSFGGRSDLIEYAKSKGIPVPVTAEKPYS
CCCCCCCCEEEEEECCHHCCCCCEEEECEEEEECCCHHHHHHHHHHCCCCEEEECCCCCC
MDRNLMHISYEGGILEDPYKEPDEKMFLLTTSPEKAPDAPEYLELDFEEGNCVAINGKKM
CCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEECCCCC
NPLEIMETLNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTILFLAHRDLESITIDRD
CHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCEEECCCCEEEEEEECCCCEEEECCC
TQHHKDKLSIEFAELIYNGHWFSSRMKAVRAFITETQRYVSGTVRIKLYKGICSVVGRKS
CCCCCHHHEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHHCCCC
QVSLYNPEMATFEKEELYNQKDAEGFINIYGLPAQETARLRKK
EEEEECCCHHCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
AQNKPVKKIVLAYSGGLDTSVILTWLKDTYGCEVIAFTADIGQKEELSGLEEKGIKTGA
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCHHHCCCCCC
SKVYIQDLRLEFARDFIFPAIQGNAIYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAH
CEEEHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GATGKGNDQVRFELGVKSLAPEKTIIAPWRIWSFGGRSDLIEYAKSKGIPVPVTAEKPYS
CCCCCCCCEEEEEECCHHCCCCCEEEECEEEEECCCHHHHHHHHHHCCCCEEEECCCCCC
MDRNLMHISYEGGILEDPYKEPDEKMFLLTTSPEKAPDAPEYLELDFEEGNCVAINGKKM
CCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCEEEECCCCC
NPLEIMETLNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTILFLAHRDLESITIDRD
CHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCEEECCCCEEEEEEECCCCEEEECCC
TQHHKDKLSIEFAELIYNGHWFSSRMKAVRAFITETQRYVSGTVRIKLYKGICSVVGRKS
CCCCCHHHEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHHCCCC
QVSLYNPEMATFEKEELYNQKDAEGFINIYGLPAQETARLRKK
EEEEECCCHHCCCHHHHCCCCCCCCEEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA