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The map label for this gene is gltA2 [H]

Identifier: 45658753

GI number: 45658753

Start: 3552803

End: 3554083

Strand: Direct

Name: gltA2 [H]

Synonym: LIC12925

Alternate gene names: 45658753

Gene position: 3552803-3554083 (Clockwise)

Preceding gene: 45658752

Following gene: 45658754

Centisome position: 83.06

GC content: 38.02

Gene sequence:

>1281_bases
ATGAGTGAATTCATTGAAATCAAAGTTGGGGAAAAATCCTATCAGTTTCCATTGATCTCCGGAACGGACGGTAAAAAAGG
AATCGATATTAGAGAACTTCATCAAAAAACCGGACTAATTTCTTACGATCCGGGTTTTTTTAACACCGCCTACGCTGTTA
GTCGAATCTCCCGAAGAGACCCGAATTCAGGTGAACTCAACTATCGTGGTTACGACATCGCAGATTTAGTACAACATTCT
ACATTTGTGGAAACTAGTTACTTATTGATTTATGGCAAACTTCCAACGGAGCAGCAGCTTAAAGATTTTTCTCTGAAATT
ATCTAAACACTCATTGATTCACGAAGATATGATCAATCTATTTGATGGATTTCCGGGTAAAGGTCATCCTCTAGCGGTTC
TGTCCGTGATGGTAACTTCTCTTTCCAGTTATTATCCAGAAGAATACGAAGAATCCTTGGACAAAGGAATCGATCATTCC
GCAAGACTGCTCGCAAAGATTAGAACTATCGCCGCCTTTTCTTACAAAAAAATCGTAGGCCAACCCTTCGTATATCCTTT
GGACAAACATCCTTATTGTACAAATTTTCTTTATATGTTATTTTCAATTCCTTCCAAGGATTATATTCCCACCGAAGACA
TAGATCGAATTTTAAATCAATTATGGATACTTTACGCGGATCATGAACAAAACGTATCCAATACAACCGTTCAAGTAATT
GGCTCTACACAAGCCAACTTATTCGCTTCTATATCTTCTGCGATCAATGCGCTTTGGGGCTCTAGAGAAGGTGGTCGTCA
GGTCGCGGCGGTAGGATTGATAGAAGACATTCTCAAATCCAGAAAATCGGTGCCGGAATATTTTGAAAAATTCAAAGGAG
ACTCGGAAAAGTTATTTGGAAACGGTTTTGGGCATAAGGCATACGAGGCAAAAAGTAGAAGAGCAATCATTGCAAGTAAA
CTGTTTCACGATTTTTACAAAAAAAATCCTATTGGACCTATCGCAGAAGTAGCTCTTAAAATCGAAGAATATATGCAAAA
TGATGAATATTATATCAATCAAAATTTGTATCCGAATTTAGAGTTCTACAGCGCGGTAATTTTTAATTCACTTGGAATAC
CAAAAGAACTTTTTACGGCTATGCAGGTAATCGGAAAACTTCCAGGCTGGTTAGCGCACTGGAGAGAACAAAGAGTTTCC
GGAAATTACAGTAAGGCTCGTCCAAAACAAATTTATTCTGGAGAAATGGGTAGAAAATACATTCCACTTTCGGAAAGATA
A

Upstream 100 bases:

>100_bases
CTCAGTTTTCCGTGTTCAATTCTCAGAATACTCGCACAAAATCGATTTTACCAAAATTAGAAGAATTGACGCGAATCATT
CTATAAGGTTCCTTATCGGT

Downstream 100 bases:

>100_bases
ATATGCAAAACCTGAGATGGAAAGATTGGCTCTCCCAAGCAGAACGTGATCTGGAATGGGCAAAAAGCGTCTTTACACTC
TGGTTTTTTTGCCCAAACTT

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSEFIEIKVGEKSYQFPLISGTDGKKGIDIRELHQKTGLISYDPGFFNTAYAVSRISRRDPNSGELNYRGYDIADLVQHS
TFVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINLFDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHS
ARLLAKIRTIAAFSYKKIVGQPFVYPLDKHPYCTNFLYMLFSIPSKDYIPTEDIDRILNQLWILYADHEQNVSNTTVQVI
GSTQANLFASISSAINALWGSREGGRQVAAVGLIEDILKSRKSVPEYFEKFKGDSEKLFGNGFGHKAYEAKSRRAIIASK
LFHDFYKKNPIGPIAEVALKIEEYMQNDEYYINQNLYPNLEFYSAVIFNSLGIPKELFTAMQVIGKLPGWLAHWREQRVS
GNYSKARPKQIYSGEMGRKYIPLSER

Sequences:

>Translated_426_residues
MSEFIEIKVGEKSYQFPLISGTDGKKGIDIRELHQKTGLISYDPGFFNTAYAVSRISRRDPNSGELNYRGYDIADLVQHS
TFVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINLFDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHS
ARLLAKIRTIAAFSYKKIVGQPFVYPLDKHPYCTNFLYMLFSIPSKDYIPTEDIDRILNQLWILYADHEQNVSNTTVQVI
GSTQANLFASISSAINALWGSREGGRQVAAVGLIEDILKSRKSVPEYFEKFKGDSEKLFGNGFGHKAYEAKSRRAIIASK
LFHDFYKKNPIGPIAEVALKIEEYMQNDEYYINQNLYPNLEFYSAVIFNSLGIPKELFTAMQVIGKLPGWLAHWREQRVS
GNYSKARPKQIYSGEMGRKYIPLSER
>Mature_425_residues
SEFIEIKVGEKSYQFPLISGTDGKKGIDIRELHQKTGLISYDPGFFNTAYAVSRISRRDPNSGELNYRGYDIADLVQHST
FVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINLFDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHSA
RLLAKIRTIAAFSYKKIVGQPFVYPLDKHPYCTNFLYMLFSIPSKDYIPTEDIDRILNQLWILYADHEQNVSNTTVQVIG
STQANLFASISSAINALWGSREGGRQVAAVGLIEDILKSRKSVPEYFEKFKGDSEKLFGNGFGHKAYEAKSRRAIIASKL
FHDFYKKNPIGPIAEVALKIEEYMQNDEYYINQNLYPNLEFYSAVIFNSLGIPKELFTAMQVIGKLPGWLAHWREQRVSG
NYSKARPKQIYSGEMGRKYIPLSER

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=425, Percent_Identity=24.9411764705882, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1786939, Length=413, Percent_Identity=36.8038740920097, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=28.5326086956522, Blast_Score=152, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324328, Length=371, Percent_Identity=26.4150943396226, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319850, Length=395, Percent_Identity=26.0759493670886, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48526; Mature: 48395

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFIEIKVGEKSYQFPLISGTDGKKGIDIRELHQKTGLISYDPGFFNTAYAVSRISRRD
CCCEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHHHHHCCC
PNSGELNYRGYDIADLVQHSTFVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINL
CCCCCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHSARLLAKIRTIAAFSYKKIVG
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
QPFVYPLDKHPYCTNFLYMLFSIPSKDYIPTEDIDRILNQLWILYADHEQNVSNTTVQVI
CCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEEE
GSTQANLFASISSAINALWGSREGGRQVAAVGLIEDILKSRKSVPEYFEKFKGDSEKLFG
ECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHCC
NGFGHKAYEAKSRRAIIASKLFHDFYKKNPIGPIAEVALKIEEYMQNDEYYINQNLYPNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCH
EFYSAVIFNSLGIPKELFTAMQVIGKLPGWLAHWREQRVSGNYSKARPKQIYSGEMGRKY
HHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCC
IPLSER
CCCCCC
>Mature Secondary Structure 
SEFIEIKVGEKSYQFPLISGTDGKKGIDIRELHQKTGLISYDPGFFNTAYAVSRISRRD
CCEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHCCCEEECCCCCHHHHHHHHHHCCC
PNSGELNYRGYDIADLVQHSTFVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINL
CCCCCCCCCCCCHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHSARLLAKIRTIAAFSYKKIVG
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
QPFVYPLDKHPYCTNFLYMLFSIPSKDYIPTEDIDRILNQLWILYADHEQNVSNTTVQVI
CCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEEE
GSTQANLFASISSAINALWGSREGGRQVAAVGLIEDILKSRKSVPEYFEKFKGDSEKLFG
ECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHCC
NGFGHKAYEAKSRRAIIASKLFHDFYKKNPIGPIAEVALKIEEYMQNDEYYINQNLYPNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCH
EFYSAVIFNSLGIPKELFTAMQVIGKLPGWLAHWREQRVSGNYSKARPKQIYSGEMGRKY
HHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCC
IPLSER
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]