Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 45658624

GI number: 45658624

Start: 3399829

End: 3400593

Strand: Reverse

Name: birA [H]

Synonym: LIC12793

Alternate gene names: 45658624

Gene position: 3400593-3399829 (Counterclockwise)

Preceding gene: 45658625

Following gene: 45658623

Centisome position: 79.51

GC content: 36.08

Gene sequence:

>765_bases
TTGGCCATGCAGAATGTATTGCTTCAACCTGAAAAAGGAATCTTTTTAGATTCCGTAACTTCTACAAACACCGTTATCAA
AGGTAAGGAATTTCCTCATGGGACTTGGATTGTAGCGGAAGAACAAACCGCTGGAAGAGGAAGGAAGGATCGAACCTGGG
AAGTTTTTGGAGAAGAATCTTTGATCTTTTCTGGAAAAATTGGTTTGGAGAATTTTGACGCGGGACCAGGACTTTCTCTT
TTTATAGGAACTGCAGTTTGTAAAACGATTCTTTCGGTATATCCTGAACTAACTCGCGAAATTAAAATTAAATGGCCGAA
CGATTTATATAGAGGCAACAAAAAAGTTGCAGGAATTCTAATAGAATCTGAAATCGAAGGTTCTTTTATGACTTTAATCA
TTGGAGTTGGATTGAACCTATATGGGAAAAACGAATCGATTCCGAATCATCTTACTGATGCGGGTTTTTTAAATACGAAT
CCAAATCGTGCAGGTAAAAAAAATGAAATTTTGGAAAAGCTAGTTCCTTTTTTGAACGAATCGATTCTTCTTTGGAAAGA
TATAGAAGGAAGAAAAAAAGAACTGATTTTGTTAAACGAATTACTTCTCTGGAAAGGACAATCCGTTCGTTATAAAGAAA
ACGGACAATTACAGACTGGAATTTTGGAAGGACTGAGTGAAAACGGTTCTTTAATTTTAAAAACTCCTTCTGGAACCAGG
TTGGATTTTATTGACAGTCCGGAGGATTTCCATTCTCTAAACTAA

Upstream 100 bases:

>100_bases
TTCAAAGAATCAAAGTTTTCAACCTAATTATCATTTTGTAGATAACGATTTAAATAAGAGTGATTTTCTACTTGCGCAAT
CCAAAGCTACTACAAAGGTA

Downstream 100 bases:

>100_bases
AATGCTTTTAGTAGTAGATGTCGGTAATACGAACACCGTTTTCGGAATTTTTGAAAATGGAAACAAGATTCCTCTCTTTC
ACAAACGAACCGTAACTAGA

Product: hypothetical protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MAMQNVLLQPEKGIFLDSVTSTNTVIKGKEFPHGTWIVAEEQTAGRGRKDRTWEVFGEESLIFSGKIGLENFDAGPGLSL
FIGTAVCKTILSVYPELTREIKIKWPNDLYRGNKKVAGILIESEIEGSFMTLIIGVGLNLYGKNESIPNHLTDAGFLNTN
PNRAGKKNEILEKLVPFLNESILLWKDIEGRKKELILLNELLLWKGQSVRYKENGQLQTGILEGLSENGSLILKTPSGTR
LDFIDSPEDFHSLN

Sequences:

>Translated_254_residues
MAMQNVLLQPEKGIFLDSVTSTNTVIKGKEFPHGTWIVAEEQTAGRGRKDRTWEVFGEESLIFSGKIGLENFDAGPGLSL
FIGTAVCKTILSVYPELTREIKIKWPNDLYRGNKKVAGILIESEIEGSFMTLIIGVGLNLYGKNESIPNHLTDAGFLNTN
PNRAGKKNEILEKLVPFLNESILLWKDIEGRKKELILLNELLLWKGQSVRYKENGQLQTGILEGLSENGSLILKTPSGTR
LDFIDSPEDFHSLN
>Mature_253_residues
AMQNVLLQPEKGIFLDSVTSTNTVIKGKEFPHGTWIVAEEQTAGRGRKDRTWEVFGEESLIFSGKIGLENFDAGPGLSLF
IGTAVCKTILSVYPELTREIKIKWPNDLYRGNKKVAGILIESEIEGSFMTLIIGVGLNLYGKNESIPNHLTDAGFLNTNP
NRAGKKNEILEKLVPFLNESILLWKDIEGRKKELILLNELLLWKGQSVRYKENGQLQTGILEGLSENGSLILKTPSGTRL
DFIDSPEDFHSLN

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=142, Percent_Identity=35.2112676056338, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24644246, Length=238, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28302; Mature: 28171

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMQNVLLQPEKGIFLDSVTSTNTVIKGKEFPHGTWIVAEEQTAGRGRKDRTWEVFGEES
CCCCCCEECCCCCEEEECCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEECCCE
LIFSGKIGLENFDAGPGLSLFIGTAVCKTILSVYPELTREIKIKWPNDLYRGNKKVAGIL
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHCCCCEEEEEE
IESEIEGSFMTLIIGVGLNLYGKNESIPNHLTDAGFLNTNPNRAGKKNEILEKLVPFLNE
EECCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
SILLWKDIEGRKKELILLNELLLWKGQSVRYKENGQLQTGILEGLSENGSLILKTPSGTR
CEEEEECCCCCCCEEEEEEHHHHHCCCCEEECCCCCEEHHHHHCCCCCCCEEEECCCCCE
LDFIDSPEDFHSLN
EEECCCCHHHCCCC
>Mature Secondary Structure 
AMQNVLLQPEKGIFLDSVTSTNTVIKGKEFPHGTWIVAEEQTAGRGRKDRTWEVFGEES
CCCCCEECCCCCEEEECCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCEEEECCCE
LIFSGKIGLENFDAGPGLSLFIGTAVCKTILSVYPELTREIKIKWPNDLYRGNKKVAGIL
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHCCCCEEEEEE
IESEIEGSFMTLIIGVGLNLYGKNESIPNHLTDAGFLNTNPNRAGKKNEILEKLVPFLNE
EECCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
SILLWKDIEGRKKELILLNELLLWKGQSVRYKENGQLQTGILEGLSENGSLILKTPSGTR
CEEEEECCCCCCCEEEEEEHHHHHCCCCEEECCCCCEEHHHHHCCCCCCCEEEECCCCCE
LDFIDSPEDFHSLN
EEECCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]