Definition Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession NC_005823
Length 4,277,185

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The map label for this gene is pnpA

Identifier: 45658536

GI number: 45658536

Start: 3286398

End: 3288494

Strand: Reverse

Name: pnpA

Synonym: LIC12701

Alternate gene names: 45658536

Gene position: 3288494-3286398 (Counterclockwise)

Preceding gene: 45658537

Following gene: 45658535

Centisome position: 76.88

GC content: 39.58

Gene sequence:

>2097_bases
ATGACACACACAATTTCCGGCCAGTATGGCCGAGATACGATCGTTTTAGAAACCGGAAGCTGGGCAAAACAAGCACACGG
AGCCGTTGTTTATAAATCTGGAAATTTAGTTTTACTTGCTACCGTATGCGCTGCTGATGAAGCAAAAGAAGGGCAGGACT
TTTTTCCACTTACTTGTGAATATACCGAAAAACTTTATTCAGTTGGTCGTTTTCCTGGTGGTTATTTTAAAAGAGAAGCC
AAACCCCCGGAACATGAAATTTTAATTTCTAGAATTATAGACAGGCCAATTCGTCCCTTATTTCCAGAAGGTTATTTCTG
CGAAGTGCAACTTCAAGTTCAAGTACTTTCTGCAGATGGGGACGTTTCTGTTGCAGGACATGCTTTAAATGCTGCAAGCG
CTGCATTAGCAGTTTCTGATATTCCCTTTAATGGCCCAATTGCAGGCGCAAGAATTGGTAGAGTCAACGGAGAATTGATT
CTAAATCCTACTACTAAAGAAATCTTAAATTCCGATTTAGATTTGGTCGTTGCCGGAACTAAAACTCACATTGTAATGAT
TGAGGGAGAAGCAAAAGAACTTAGTAATGAAGAAATGATTGCTGCTCTTCGTTTTGCTCAAAAACATATTGCAGAATTTG
TAACTCTTCAAGAAGAATATGCGAAAAAAATCGGAGTCGTCAAACGCGAAGTTAAATTGAAAGTTCGGGATGAAGAACTT
CTTTCTAAGGTAAAAGAATATGCGTTTGCAAAACTAACTACGGCTAATCAAACTCCAGATAAAACTGCACGTAATAAAGA
AATTTCTAATGTAAATAAGGAAGTAGTAGAATTTTTCAAAGATACGATTGAAGACTCTGACAAGATTAAGGATATAAAAG
CATATCTTCACGAATTGGAATATGAAATTGTAAGAGAACAAGTTCTTACAAAAGGAACTCGTTTTGATGGTAGAAAGTTA
GACGAAATCCGTTCTATTTCCGTGGAAATCAATCCTCTTCCCGGTCCTCATGGTTCTGCAGTTTTTACGAGAGGGCAGAC
TCAGTCTTTGGGAGTTGTGACTTTAGGGACCGGTTCTGATAATCAAAGATACGAAACTTTAGAAGGTCAGAAAGAAAAAT
CTTTCATGCTACATTATAATTTTCCCGCGTTTTCTGTGGGGGAAGTTCGTAGATCTTCCGGTCCAGGTAGAAGGGAAATC
GGTCATGGAAATCTCGCAGAACGTGCGTTAAAACTTGTTCTTCCTAAACCGGATGAGTTTCCTTATGTAATCCGGGTTGT
ATCTGAAATTTTAGAATCCAACGGCTCCAGTTCTATGGCTTCTGTTTGTTCCGGTTCTTTGGCGCTTATGGCTGCGGGTG
TTCCGATTCAGGGAAGTGTTTCTGGAATTGCAATGGGGCTTTTTTCTGATTCTTCCGGTAAGTATGCAGTTTTATCCGAT
ATTGCGGGTCTGGAAGACCATTTTGGTGATATGGATTGTAAAATCGCCGGAACCAGAAAAGGAATTACCGCGTTTCAAAT
GGATTTGAAAGTGACTGGTGTCAGCTTCGATGTTTTGGAAAGCGTTTTCGAACAAGCACAGAGAGGTAGATTTCATATTT
TGGATATTATGGAGAAACATATATCCAAGGCTTCCTCGACGTTAGCCGGAACTGCGCCTCGTATCATCGTTAGAAATATT
CCTAAAGATAGAATCGGGGAATTGATCGGGCCTGGTGGCAAAAACGTTAGAGGGATTAGCGAACTTACCGGAGCTGAACT
TTATATAGAAGACGATGGAAAAGTGACTATCTCTGGTTCCAACCAAGAGTCCGCAGAAAAAGCGGCCAAAATGGTAGATG
GGTTTTTTGCAGAGGTAGAAGTAGGAAAAATTTACGAAGGAAAAGTAAAACGAATCGCCGATTTCGGTGCATTTGTGGAA
ATTCTTCCAGGTAAAGAAGGCCTTTGCCATATTTCCAAGATCGATTTTAAAAGAGTAAATTCGGTCAAAGATATAGTTAA
AGAAGGCGATATTATTCGAGTGAAAGTTTTAAACGTAGATAAAACCGGAAAAATTGATCTTTCCAGAAAAGACGCTCTCG
AAGAAGAACAAGTATAA

Upstream 100 bases:

>100_bases
GCAAAAGAAAAAAACTTTTAGATTATCTTAAAAGAACTGAATTAGAACGTTATAAAAAACTAATCGAAACTCTCGGACTT
CGTAAGTAAGAGAGTCGTTC

Downstream 100 bases:

>100_bases
GATTTTTTTAATATTCCATTGATGCAGGAACCTTCACAGATAGTTCATAGAAAAGTTCTACCTGGCGGAATAACGGTTCT
TTTTCAAAAAGCTCCTCATA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MTHTISGQYGRDTIVLETGSWAKQAHGAVVYKSGNLVLLATVCAADEAKEGQDFFPLTCEYTEKLYSVGRFPGGYFKREA
KPPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADGDVSVAGHALNAASAALAVSDIPFNGPIAGARIGRVNGELI
LNPTTKEILNSDLDLVVAGTKTHIVMIEGEAKELSNEEMIAALRFAQKHIAEFVTLQEEYAKKIGVVKREVKLKVRDEEL
LSKVKEYAFAKLTTANQTPDKTARNKEISNVNKEVVEFFKDTIEDSDKIKDIKAYLHELEYEIVREQVLTKGTRFDGRKL
DEIRSISVEINPLPGPHGSAVFTRGQTQSLGVVTLGTGSDNQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREI
GHGNLAERALKLVLPKPDEFPYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIQGSVSGIAMGLFSDSSGKYAVLSD
IAGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKHISKASSTLAGTAPRIIVRNI
PKDRIGELIGPGGKNVRGISELTGAELYIEDDGKVTISGSNQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVE
ILPGKEGLCHISKIDFKRVNSVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEEQV

Sequences:

>Translated_698_residues
MTHTISGQYGRDTIVLETGSWAKQAHGAVVYKSGNLVLLATVCAADEAKEGQDFFPLTCEYTEKLYSVGRFPGGYFKREA
KPPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADGDVSVAGHALNAASAALAVSDIPFNGPIAGARIGRVNGELI
LNPTTKEILNSDLDLVVAGTKTHIVMIEGEAKELSNEEMIAALRFAQKHIAEFVTLQEEYAKKIGVVKREVKLKVRDEEL
LSKVKEYAFAKLTTANQTPDKTARNKEISNVNKEVVEFFKDTIEDSDKIKDIKAYLHELEYEIVREQVLTKGTRFDGRKL
DEIRSISVEINPLPGPHGSAVFTRGQTQSLGVVTLGTGSDNQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREI
GHGNLAERALKLVLPKPDEFPYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIQGSVSGIAMGLFSDSSGKYAVLSD
IAGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKHISKASSTLAGTAPRIIVRNI
PKDRIGELIGPGGKNVRGISELTGAELYIEDDGKVTISGSNQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVE
ILPGKEGLCHISKIDFKRVNSVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEEQV
>Mature_697_residues
THTISGQYGRDTIVLETGSWAKQAHGAVVYKSGNLVLLATVCAADEAKEGQDFFPLTCEYTEKLYSVGRFPGGYFKREAK
PPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADGDVSVAGHALNAASAALAVSDIPFNGPIAGARIGRVNGELIL
NPTTKEILNSDLDLVVAGTKTHIVMIEGEAKELSNEEMIAALRFAQKHIAEFVTLQEEYAKKIGVVKREVKLKVRDEELL
SKVKEYAFAKLTTANQTPDKTARNKEISNVNKEVVEFFKDTIEDSDKIKDIKAYLHELEYEIVREQVLTKGTRFDGRKLD
EIRSISVEINPLPGPHGSAVFTRGQTQSLGVVTLGTGSDNQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREIG
HGNLAERALKLVLPKPDEFPYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIQGSVSGIAMGLFSDSSGKYAVLSDI
AGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKHISKASSTLAGTAPRIIVRNIP
KDRIGELIGPGGKNVRGISELTGAELYIEDDGKVTISGSNQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVEI
LPGKEGLCHISKIDFKRVNSVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEEQV

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=712, Percent_Identity=35.6741573033708, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=46.5317919075145, Blast_Score=616, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI115534063, Length=705, Percent_Identity=34.7517730496454, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI281362905, Length=709, Percent_Identity=36.8124118476728, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=709, Percent_Identity=36.8124118476728, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=709, Percent_Identity=36.8124118476728, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=653, Percent_Identity=37.5191424196018, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_LEPIC (Q72NX7)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002622.1
- HSSP:   P05055
- ProteinModelPortal:   Q72NX7
- SMR:   Q72NX7
- GeneID:   2772003
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12701
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   LINT267671:LIC_12701-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76297; Mature: 76166

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MTHTISGQYGRDTIVLETGSWAKQAHGAVVYKSGNLVLLATVCAADEAKEGQDFFPLTCE
CCCCCCCCCCCCEEEEECCCCHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEEHH
YTEKLYSVGRFPGGYFKREAKPPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCC
DVSVAGHALNAASAALAVSDIPFNGPIAGARIGRVNGELILNPTTKEILNSDLDLVVAGT
CEEEECCHHHHHHHEEEEECCCCCCCCCCCEEEECCCEEEECCCHHHHCCCCCCEEEECC
KTHIVMIEGEAKELSNEEMIAALRFAQKHIAEFVTLQEEYAKKIGVVKREVKLKVRDEEL
CEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECHHHH
LSKVKEYAFAKLTTANQTPDKTARNKEISNVNKEVVEFFKDTIEDSDKIKDIKAYLHELE
HHHHHHHHHHEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
YEIVREQVLTKGTRFDGRKLDEIRSISVEINPLPGPHGSAVFTRGQTQSLGVVTLGTGSD
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCC
NQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREIGHGNLAERALKLVLPKPDEF
CCCCHHCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
PYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIQGSVSGIAMGLFSDSSGKYAVLSD
HHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEECCCCCEEEHHH
IAGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKH
HCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHH
ISKASSTLAGTAPRIIVRNIPKDRIGELIGPGGKNVRGISELTGAELYIEDDGKVTISGS
HHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCHHHCCCCEEEEECCCEEEEECC
NQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVEILPGKEGLCHISKIDFKRVN
CHHHHHHHHHHHHHHHHEEEECCEECCHHHHHHHHCCCEEECCCCCCCEEEECCCHHHHH
SVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEEQV
HHHHHHHCCCEEEEEEEECCCCCCCCCCHHHCCHHCCC
>Mature Secondary Structure 
THTISGQYGRDTIVLETGSWAKQAHGAVVYKSGNLVLLATVCAADEAKEGQDFFPLTCE
CCCCCCCCCCCEEEEECCCCHHHCCCEEEEECCCEEEEEEEECCCCCCCCCCCCCEEHH
YTEKLYSVGRFPGGYFKREAKPPEHEILISRIIDRPIRPLFPEGYFCEVQLQVQVLSADG
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCC
DVSVAGHALNAASAALAVSDIPFNGPIAGARIGRVNGELILNPTTKEILNSDLDLVVAGT
CEEEECCHHHHHHHEEEEECCCCCCCCCCCEEEECCCEEEECCCHHHHCCCCCCEEEECC
KTHIVMIEGEAKELSNEEMIAALRFAQKHIAEFVTLQEEYAKKIGVVKREVKLKVRDEEL
CEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECHHHH
LSKVKEYAFAKLTTANQTPDKTARNKEISNVNKEVVEFFKDTIEDSDKIKDIKAYLHELE
HHHHHHHHHHEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
YEIVREQVLTKGTRFDGRKLDEIRSISVEINPLPGPHGSAVFTRGQTQSLGVVTLGTGSD
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCEEEEECCCCC
NQRYETLEGQKEKSFMLHYNFPAFSVGEVRRSSGPGRREIGHGNLAERALKLVLPKPDEF
CCCCHHCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
PYVIRVVSEILESNGSSSMASVCSGSLALMAAGVPIQGSVSGIAMGLFSDSSGKYAVLSD
HHHHHHHHHHHHCCCCCHHHHHHCCCEEEEEECCCCCCCCCEEEEEEEECCCCCEEEHHH
IAGLEDHFGDMDCKIAGTRKGITAFQMDLKVTGVSFDVLESVFEQAQRGRFHILDIMEKH
HCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHH
ISKASSTLAGTAPRIIVRNIPKDRIGELIGPGGKNVRGISELTGAELYIEDDGKVTISGS
HHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCHHHCCCCEEEEECCCEEEEECC
NQESAEKAAKMVDGFFAEVEVGKIYEGKVKRIADFGAFVEILPGKEGLCHISKIDFKRVN
CHHHHHHHHHHHHHHHHEEEECCEECCHHHHHHHHCCCEEECCCCCCCEEEECCCHHHHH
SVKDIVKEGDIIRVKVLNVDKTGKIDLSRKDALEEEQV
HHHHHHHCCCEEEEEEEECCCCCCCCCCHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA