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The map label for this gene is dut

Identifier: 45658401

GI number: 45658401

Start: 3102519

End: 3102956

Strand: Reverse

Name: dut

Synonym: LIC12560

Alternate gene names: 45658401

Gene position: 3102956-3102519 (Counterclockwise)

Preceding gene: 45658403

Following gene: 45658400

Centisome position: 72.55

GC content: 37.9

Gene sequence:

>438_bases
ATGAAAATTTTTGTACAAAAACTGAGACCAAACGCCGAGCTTCCTTTATTACAGACAAAACAGGCGGCTGGTTATGATAT
TCATGCTTGTTTAGATTCCAAATTGGTTCTAGAACCAGGTAACGTTGGTTTAGTTCCTACGGGTCTTTCCTTTGCCATTC
CTCAAGAGTTTCATTTTGAAATTAGACCTAGATCTGGTTTCTCTACAAAAAATAGAATCTTAATTCCAAATTCACCCGGA
ACTATCGATAGTGATTACAGAGGTGAATTGATGATTCCTCTTTTGAATTTAGGAGATTCTTCTTTTATAATTGAACATGG
AATGAGAATCGCTCAATTACTGATCCGTAAAACTTGGTATGCGGATTGGGAGTTAGTCTCGGAATTTGCAGATCGGACGG
AAAGAGGTGCAAACGGTTTTGGTTCTACCGGACATTAA

Upstream 100 bases:

>100_bases
ATGAATTGGTTCATTTTATTTCTGATTTTTTCAAAACGAAAATAGTTTTTTAAAATATTGTCCTATTTTTTTATAAGGTA
AAAATGGGGTATCCTCATCT

Downstream 100 bases:

>100_bases
ACTGTATTCAAATTGATTTATTAATAGCTTATCCCAATAAACATCGCCCCCGTTTGCTGGTTACTTCAAACGCCTAAAAC
GTGACCTATCTCGTGACCCG

Product: deoxyuridine 5'triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKIFVQKLRPNAELPLLQTKQAAGYDIHACLDSKLVLEPGNVGLVPTGLSFAIPQEFHFEIRPRSGFSTKNRILIPNSPG
TIDSDYRGELMIPLLNLGDSSFIIEHGMRIAQLLIRKTWYADWELVSEFADRTERGANGFGSTGH

Sequences:

>Translated_145_residues
MKIFVQKLRPNAELPLLQTKQAAGYDIHACLDSKLVLEPGNVGLVPTGLSFAIPQEFHFEIRPRSGFSTKNRILIPNSPG
TIDSDYRGELMIPLLNLGDSSFIIEHGMRIAQLLIRKTWYADWELVSEFADRTERGANGFGSTGH
>Mature_145_residues
MKIFVQKLRPNAELPLLQTKQAAGYDIHACLDSKLVLEPGNVGLVPTGLSFAIPQEFHFEIRPRSGFSTKNRILIPNSPG
TIDSDYRGELMIPLLNLGDSSFIIEHGMRIAQLLIRKTWYADWELVSEFADRTERGANGFGSTGH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=144, Percent_Identity=36.1111111111111, Blast_Score=93, Evalue=7e-20,
Organism=Homo sapiens, GI4503423, Length=144, Percent_Identity=36.1111111111111, Blast_Score=93, Evalue=7e-20,
Organism=Homo sapiens, GI70906441, Length=144, Percent_Identity=36.1111111111111, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=42.5675675675676, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71988561, Length=139, Percent_Identity=37.410071942446, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319729, Length=145, Percent_Identity=36.551724137931, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24583610, Length=138, Percent_Identity=37.6811594202899, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921126, Length=138, Percent_Identity=37.6811594202899, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_LEPIC (P61909)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002487.1
- ProteinModelPortal:   P61909
- SMR:   P61909
- GeneID:   2771885
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12560
- HOGENOM:   HBG436079
- OMA:   GTIDEGY
- ProtClustDB:   CLSK573773
- BioCyc:   LINT-130-01:LINT-130-01-002487-MONOMER
- BioCyc:   LINT267671:LIC_12560-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16182; Mature: 16182

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: BINDING 77-77 BINDING 91-91

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFVQKLRPNAELPLLQTKQAAGYDIHACLDSKLVLEPGNVGLVPTGLSFAIPQEFHFE
CCEEEEECCCCCCCCEEECCCCCCCEEEEECCCEEEECCCCCEEEECCCEEECCCCEEEE
IRPRSGFSTKNRILIPNSPGTIDSDYRGELMIPLLNLGDSSFIIEHGMRIAQLLIRKTWY
ECCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
ADWELVSEFADRTERGANGFGSTGH
CCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIFVQKLRPNAELPLLQTKQAAGYDIHACLDSKLVLEPGNVGLVPTGLSFAIPQEFHFE
CCEEEEECCCCCCCCEEECCCCCCCEEEEECCCEEEECCCCCEEEECCCEEECCCCEEEE
IRPRSGFSTKNRILIPNSPGTIDSDYRGELMIPLLNLGDSSFIIEHGMRIAQLLIRKTWY
ECCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
ADWELVSEFADRTERGANGFGSTGH
CCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA