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The map label for this gene is rimO

Identifier: 45658385

GI number: 45658385

Start: 3081501

End: 3082814

Strand: Reverse

Name: rimO

Synonym: LIC12543

Alternate gene names: 45658385

Gene position: 3082814-3081501 (Counterclockwise)

Preceding gene: 45658386

Following gene: 45658384

Centisome position: 72.08

GC content: 39.57

Gene sequence:

>1314_bases
TTGGATAAGAAGTTCTACATCACCACACTCGGTTGTCCTAAAAATATTGCGGACTCTATGAGTATGCATCATTCTCTTCT
GGAAGAAGGGTTTACTCCCGCTTCTTTGCCTGAAGAATCCGATTTCCATTTTATCAATACCTGTACGTTTATTCAATCAG
CTACGGAAGAAACGATTCAGACCATTTTGTCTGCGGCTCAGGTAAAAAAACAGAATCACCAAAAGTTAGTTGTAGTAGGT
TGTTTTGCAGAACGTTATCCGGACAATATTCATTCTGAAATTCCGGAAGTGGATTTGTTTTTTGGAACTGGCAAATACTC
ACAAGCAGGAAAAATTTTACGCGAAAAATTTCCGGAACTTTCTCCTTCTCAACTGGAATTTAACGATAGTTTATTAGAAA
GATGGAAATTATCTTCTAAAATCGAAAACTATTCTAAACCGTATGCTTATGTAAAAGTTTCTGACGGTTGTAATCGGGGT
TGTTCTTTTTGTATCATTCCTTCCTTTCGAGGAAAATTCGCAGAATCTCCGTTAGACGATATTTTAAGAGATACAAATAG
AGCGATACGCGCGGGTGCCAAAGAAATCTGTTTGGTTTCTCAAGATACGGTCTATTACGGAAGAGACTCCGAAATACTTT
TGGATATGGTTCGTAAAGTTGCAGAGATTGATTCTCTCGAAATTCTAAGATTATTGTATTTGTATCCGGATAAAAAAACC
GAAAAGTTGATTCGTTTGATGGGAGAAACTTCTAAGATTGCTCCGTATTTGGAATCCCCTCTACAACACGTATCTTCTAA
AATTCTAAAGGTGATGAACCGGACCGGAGAAAGTTCCTATTTTAAGGATCTATTCTCTCTTGCCAGAGAAGTCAAACCAG
GTTTAGAAATTCGTACTTCGTTTATCATCGGTTATCCGGGAGAAGAACCCGAAGACGTGGATCAGATTTTACGTTTTATC
GAAGATACCAGACCGGAAAAAGTAAATTTATTTTCTTATTCCCCTCAAGAAGGAACCAAAGGTGCCCAACTCAAACAAAC
CGTTTCCGAAAAGGAAAAATCGAAACGAATCAATCTGATCCGTGATTCTCATCTGGAGATCTTAGAAGAGATACACGAAT
CTAGAATTGGTCGGACCTACGATGCGATTGTGGACGGGATAGAAGACGGACAGGCGGTGGTTCGTAGATTTCAAGACGCT
CCGGAAATGGATGAAGTAGTCTACGTGGACGATGTTTCTCTCCTTCCGGGTAGGATCGGAAAAGTGCGGATCGATTCCTT
TTACGAATACGACATGAATGGAACTTGGGTATGA

Upstream 100 bases:

>100_bases
GACGCCCTGGTAAACTAGTGAAGAAAATTTTTGTGAAAACTCAAAATCCTTATGATAGCACACAGTCCATCATCAAGGAG
TTGGGAGAATAGGAATTTTT

Downstream 100 bases:

>100_bases
ACAAGGTTATTTTCAATATTCCTAATATTCTTACTATGTTAAGAGTTGCTGCGGTGCCTTTTTTCGTATGGTTTTTATTT
CAAAAGGAATTGGAATATCA

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MDKKFYITTLGCPKNIADSMSMHHSLLEEGFTPASLPEESDFHFINTCTFIQSATEETIQTILSAAQVKKQNHQKLVVVG
CFAERYPDNIHSEIPEVDLFFGTGKYSQAGKILREKFPELSPSQLEFNDSLLERWKLSSKIENYSKPYAYVKVSDGCNRG
CSFCIIPSFRGKFAESPLDDILRDTNRAIRAGAKEICLVSQDTVYYGRDSEILLDMVRKVAEIDSLEILRLLYLYPDKKT
EKLIRLMGETSKIAPYLESPLQHVSSKILKVMNRTGESSYFKDLFSLAREVKPGLEIRTSFIIGYPGEEPEDVDQILRFI
EDTRPEKVNLFSYSPQEGTKGAQLKQTVSEKEKSKRINLIRDSHLEILEEIHESRIGRTYDAIVDGIEDGQAVVRRFQDA
PEMDEVVYVDDVSLLPGRIGKVRIDSFYEYDMNGTWV

Sequences:

>Translated_437_residues
MDKKFYITTLGCPKNIADSMSMHHSLLEEGFTPASLPEESDFHFINTCTFIQSATEETIQTILSAAQVKKQNHQKLVVVG
CFAERYPDNIHSEIPEVDLFFGTGKYSQAGKILREKFPELSPSQLEFNDSLLERWKLSSKIENYSKPYAYVKVSDGCNRG
CSFCIIPSFRGKFAESPLDDILRDTNRAIRAGAKEICLVSQDTVYYGRDSEILLDMVRKVAEIDSLEILRLLYLYPDKKT
EKLIRLMGETSKIAPYLESPLQHVSSKILKVMNRTGESSYFKDLFSLAREVKPGLEIRTSFIIGYPGEEPEDVDQILRFI
EDTRPEKVNLFSYSPQEGTKGAQLKQTVSEKEKSKRINLIRDSHLEILEEIHESRIGRTYDAIVDGIEDGQAVVRRFQDA
PEMDEVVYVDDVSLLPGRIGKVRIDSFYEYDMNGTWV
>Mature_437_residues
MDKKFYITTLGCPKNIADSMSMHHSLLEEGFTPASLPEESDFHFINTCTFIQSATEETIQTILSAAQVKKQNHQKLVVVG
CFAERYPDNIHSEIPEVDLFFGTGKYSQAGKILREKFPELSPSQLEFNDSLLERWKLSSKIENYSKPYAYVKVSDGCNRG
CSFCIIPSFRGKFAESPLDDILRDTNRAIRAGAKEICLVSQDTVYYGRDSEILLDMVRKVAEIDSLEILRLLYLYPDKKT
EKLIRLMGETSKIAPYLESPLQHVSSKILKVMNRTGESSYFKDLFSLAREVKPGLEIRTSFIIGYPGEEPEDVDQILRFI
EDTRPEKVNLFSYSPQEGTKGAQLKQTVSEKEKSKRINLIRDSHLEILEEIHESRIGRTYDAIVDGIEDGQAVVRRFQDA
PEMDEVVYVDDVSLLPGRIGKVRIDSFYEYDMNGTWV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=426, Percent_Identity=23.2394366197183, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI28872782, Length=426, Percent_Identity=23.2394366197183, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI93277076, Length=370, Percent_Identity=23.7837837837838, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=34.0807174887892, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=25.7777777777778, Blast_Score=165, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=380, Percent_Identity=21.5789473684211, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996771, Length=241, Percent_Identity=25.3112033195021, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922432, Length=393, Percent_Identity=23.4096692111959, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356207, Length=418, Percent_Identity=21.7703349282297, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_LEPIC (Q72PC8)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002471.1
- ProteinModelPortal:   Q72PC8
- SMR:   Q72PC8
- GeneID:   2772312
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12543
- NMPDR:   fig|267671.1.peg.2471
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK573783
- BioCyc:   LINT267671:LIC_12543-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49885; Mature: 49885

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKFYITTLGCPKNIADSMSMHHSLLEEGFTPASLPEESDFHFINTCTFIQSATEETIQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
TILSAAQVKKQNHQKLVVVGCFAERYPDNIHSEIPEVDLFFGTGKYSQAGKILREKFPEL
HHHHHHHHHHCCCCEEEEEEECHHHCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCC
SPSQLEFNDSLLERWKLSSKIENYSKPYAYVKVSDGCNRGCSFCIIPSFRGKFAESPLDD
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCHHH
ILRDTNRAIRAGAKEICLVSQDTVYYGRDSEILLDMVRKVAEIDSLEILRLLYLYPDKKT
HHHHHHHHHHCCCHHEEEEECCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
EKLIRLMGETSKIAPYLESPLQHVSSKILKVMNRTGESSYFKDLFSLAREVKPGLEIRTS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEE
FIIGYPGEEPEDVDQILRFIEDTRPEKVNLFSYSPQEGTKGAQLKQTVSEKEKSKRINLI
EEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RDSHLEILEEIHESRIGRTYDAIVDGIEDGQAVVRRFQDAPEMDEVVYVDDVSLLPGRIG
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHCCCCCC
KVRIDSFYEYDMNGTWV
EEEECCEEEECCCCCCC
>Mature Secondary Structure
MDKKFYITTLGCPKNIADSMSMHHSLLEEGFTPASLPEESDFHFINTCTFIQSATEETIQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
TILSAAQVKKQNHQKLVVVGCFAERYPDNIHSEIPEVDLFFGTGKYSQAGKILREKFPEL
HHHHHHHHHHCCCCEEEEEEECHHHCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCC
SPSQLEFNDSLLERWKLSSKIENYSKPYAYVKVSDGCNRGCSFCIIPSFRGKFAESPLDD
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCHHH
ILRDTNRAIRAGAKEICLVSQDTVYYGRDSEILLDMVRKVAEIDSLEILRLLYLYPDKKT
HHHHHHHHHHCCCHHEEEEECCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
EKLIRLMGETSKIAPYLESPLQHVSSKILKVMNRTGESSYFKDLFSLAREVKPGLEIRTS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEE
FIIGYPGEEPEDVDQILRFIEDTRPEKVNLFSYSPQEGTKGAQLKQTVSEKEKSKRINLI
EEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RDSHLEILEEIHESRIGRTYDAIVDGIEDGQAVVRRFQDAPEMDEVVYVDDVSLLPGRIG
HHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCHHCCCCCC
KVRIDSFYEYDMNGTWV
EEEECCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA