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The map label for this gene is cysT

Identifier: 45658370

GI number: 45658370

Start: 3064823

End: 3065668

Strand: Reverse

Name: cysT

Synonym: LIC12528

Alternate gene names: 45658370

Gene position: 3065668-3064823 (Counterclockwise)

Preceding gene: 45658371

Following gene: 45658369

Centisome position: 71.67

GC content: 37.71

Gene sequence:

>846_bases
TTGAAAAGAGAAAACGTGGCCACTTTTAGGACAAAACCTTACGGAAAAACTGCCTTTGGGATTACATTAGGCGTAACAGT
AACTTATCTAAGTTTAATCGTTATCATTCCGTTAGGTGCTCTTTTTCTAAAAAGTACTGGAATTGGCTGGGAAGGTTTTT
GGAAGTTACTTACCCACGAAAGGATTTTAGCTGCTCTCTATTTAAGTTTTGGAGCTGGGGCGACTGCGGCAATTCTCAAT
TTGTTTATTGGTTTTTTATTTGCTTGGGTATTAGTACGTTATAATTTTCCTGGAAAGTCTTTACTTGATTCTCTAGTAGA
TCTTCCGTTTACTTTGCCGACTGCAGTTGCCGGAATTGCTCTTACTACAATTTATACTCCAAACGGAATCATTGGAAAAT
TTTTCGAACCATTTGGAATCAAAATTGCATATACTCCAATTGGAATCGTTATTGCTCTCGTGTTTATAGGTTTCCCGTTT
GTAGTCAGAACCGTTCAACCGGTTTTAGAAGAGTTTCCTAAAGAATTGGAAGAATCTGCCTATTGTTTAGGAGCAAACCG
CTGGCAGATATTTAGAAGAGTAATTTTTCCAGAACTTATCTCTCCTCTGGTTACTGGAGCCGCAATGGCATTTGCAAGAG
GAATTGGAGAATACGGTTCTGTAGTGTTTATATCTGGGAACCTTCCCGGTAAAACGGAAATTCTTCCGTTGTTGATCGTC
ACAAAATTGGAACAATACGAATACGAAGAAGCGACCGGAATTGCGTTTATTATGCTTGTAATTTCATTTAGTATATTATT
ATGTATTAATGTTATTCAGAACATCGCTGCTAGGAAAAGCGAATGA

Upstream 100 bases:

>100_bases
CGTAGCTGGTTCTTGGAAAAATGCTCAGGAAAAACATTTTTCTGACGGAGCCATTTTTGATCAGATTACTTCAAAACAAT
GAATAAAAAAATTCTTAGAT

Downstream 100 bases:

>100_bases
AACAAGAATCGTTAATCGTTAGAAGTTTTCTTATCGGTGTCGTTTTTTTTCTCACCGTTTTGATTTTGATTCTTCCTATT
TATACCGTCTTTCAAGAAGC

Product: sulfate ABC transport system permease protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKRENVATFRTKPYGKTAFGITLGVTVTYLSLIVIIPLGALFLKSTGIGWEGFWKLLTHERILAALYLSFGAGATAAILN
LFIGFLFAWVLVRYNFPGKSLLDSLVDLPFTLPTAVAGIALTTIYTPNGIIGKFFEPFGIKIAYTPIGIVIALVFIGFPF
VVRTVQPVLEEFPKELEESAYCLGANRWQIFRRVIFPELISPLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIV
TKLEQYEYEEATGIAFIMLVISFSILLCINVIQNIAARKSE

Sequences:

>Translated_281_residues
MKRENVATFRTKPYGKTAFGITLGVTVTYLSLIVIIPLGALFLKSTGIGWEGFWKLLTHERILAALYLSFGAGATAAILN
LFIGFLFAWVLVRYNFPGKSLLDSLVDLPFTLPTAVAGIALTTIYTPNGIIGKFFEPFGIKIAYTPIGIVIALVFIGFPF
VVRTVQPVLEEFPKELEESAYCLGANRWQIFRRVIFPELISPLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIV
TKLEQYEYEEATGIAFIMLVISFSILLCINVIQNIAARKSE
>Mature_281_residues
MKRENVATFRTKPYGKTAFGITLGVTVTYLSLIVIIPLGALFLKSTGIGWEGFWKLLTHERILAALYLSFGAGATAAILN
LFIGFLFAWVLVRYNFPGKSLLDSLVDLPFTLPTAVAGIALTTIYTPNGIIGKFFEPFGIKIAYTPIGIVIALVFIGFPF
VVRTVQPVLEEFPKELEESAYCLGANRWQIFRRVIFPELISPLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIV
TKLEQYEYEEATGIAFIMLVISFSILLCINVIQNIAARKSE

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=261, Percent_Identity=43.6781609195402, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI87082099, Length=238, Percent_Identity=34.0336134453782, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1786980, Length=218, Percent_Identity=34.4036697247706, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786254, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787368, Length=240, Percent_Identity=27.9166666666667, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787369, Length=171, Percent_Identity=27.4853801169591, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30858; Mature: 30858

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRENVATFRTKPYGKTAFGITLGVTVTYLSLIVIIPLGALFLKSTGIGWEGFWKLLTHE
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RILAALYLSFGAGATAAILNLFIGFLFAWVLVRYNFPGKSLLDSLVDLPFTLPTAVAGIA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHH
LTTIYTPNGIIGKFFEPFGIKIAYTPIGIVIALVFIGFPFVVRTVQPVLEEFPKELEESA
HHEEECCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
YCLGANRWQIFRRVIFPELISPLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
TKLEQYEYEEATGIAFIMLVISFSILLCINVIQNIAARKSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRENVATFRTKPYGKTAFGITLGVTVTYLSLIVIIPLGALFLKSTGIGWEGFWKLLTHE
CCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RILAALYLSFGAGATAAILNLFIGFLFAWVLVRYNFPGKSLLDSLVDLPFTLPTAVAGIA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHH
LTTIYTPNGIIGKFFEPFGIKIAYTPIGIVIALVFIGFPFVVRTVQPVLEEFPKELEESA
HHEEECCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
YCLGANRWQIFRRVIFPELISPLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHH
TKLEQYEYEEATGIAFIMLVISFSILLCINVIQNIAARKSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]